CN110249082B - 测定蛋白质的方法 - Google Patents

测定蛋白质的方法 Download PDF

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Publication number
CN110249082B
CN110249082B CN201780085386.2A CN201780085386A CN110249082B CN 110249082 B CN110249082 B CN 110249082B CN 201780085386 A CN201780085386 A CN 201780085386A CN 110249082 B CN110249082 B CN 110249082B
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proteins
protein
substrate
affinity
affinity reagents
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CN110249082A (zh
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帕拉格·马利克
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Nordilus Subsidiary
Nautilus Subsidiary Inc
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Nordilus Subsidiary
Ignite Biosciences Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN201780085386.2A 2016-12-01 2017-12-01 测定蛋白质的方法 Active CN110249082B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662429063P 2016-12-01 2016-12-01
US62/429,063 2016-12-01
US201762500455P 2017-05-02 2017-05-02
US62/500,455 2017-05-02
PCT/US2017/064322 WO2018102759A1 (en) 2016-12-01 2017-12-01 Methods of assaying proteins

Publications (2)

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CN110249082A CN110249082A (zh) 2019-09-17
CN110249082B true CN110249082B (zh) 2023-07-07

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US (14) US10473654B1 (enExample)
EP (3) EP3548652B1 (enExample)
JP (3) JP2020514746A (enExample)
CN (1) CN110249082B (enExample)
DK (1) DK3548652T3 (enExample)
WO (1) WO2018102759A1 (enExample)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
WO2016069124A1 (en) 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
GB2577626B (en) 2011-06-23 2020-09-23 Univ Texas Identifying peptides at the single molecule level
JP2020514746A (ja) 2016-12-01 2020-05-21 ノーティラス バイオテクノロジー インコーポレイテッド タンパク質をアッセイする方法
WO2019036055A2 (en) 2017-08-18 2019-02-21 Ignite Biosciences, Inc. METHODS OF SELECTING BINDING REAGENTS
US11721412B2 (en) 2017-10-23 2023-08-08 Nautilus Subsidiary, Inc. Methods for identifying a protein in a sample of unknown proteins
CN112154230A (zh) * 2017-10-23 2020-12-29 诺迪勒思生物科技公司 用于蛋白质鉴定的方法和系统
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
CA3086915A1 (en) 2017-12-29 2019-07-04 Sujal M. Patel Decoding approaches for protein identification
CN112236528A (zh) 2018-04-04 2021-01-15 诺迪勒思生物科技公司 产生纳米阵列和微阵列的方法
WO2019236749A2 (en) 2018-06-06 2019-12-12 Ignite Biosciences, Inc. Methods and applications of protein identification
AU2019301750A1 (en) 2018-07-12 2021-01-28 Board Of Regents, The University Of Texas System Molecular neighborhood detection by oligonucleotides
EP3841234B1 (en) 2018-08-23 2023-10-04 ProteinSimple Systems and methods for the detection and analysis of free thiol
WO2020072907A1 (en) 2018-10-05 2020-04-09 Board Of Regents, The University Of Texas System Solid-phase n-terminal peptide capture and release
AU2019374692B2 (en) 2018-11-07 2025-08-28 Seer, Inc. Compositions, methods and systems for protein corona analysis and uses thereof
JP2022513092A (ja) 2018-11-20 2022-02-07 ノーティラス・バイオテクノロジー・インコーポレイテッド 親和性試薬の設計および選択
JP7518852B2 (ja) 2019-03-26 2024-07-18 シアー， インコーポレイテッド 生物流体からのタンパク質コロナ分析のための組成物、方法およびシステム、ならびにそれらの使用
AU2020266136B2 (en) 2019-04-29 2026-04-02 Nautilus Subsidiary, Inc. Methods and systems for integrated on-chip single-molecule detection
WO2021087402A1 (en) 2019-10-30 2021-05-06 Nautilus Biotechnology, Inc. Flow cell systems and methods
EP4055160B1 (en) 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
CN115279919A (zh) 2020-01-13 2022-11-01 贝克顿迪金森公司 使用含有du的寡核苷酸的细胞捕获
US20210246492A1 (en) * 2020-02-12 2021-08-12 Becton, Dickinson And Company Intracellular abseq
CN111289425B (zh) * 2020-03-12 2021-03-30 中国计量科学研究院 基于荧光标记流式单分子计数的蛋白质含量计量基准方法
GB202004469D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Controls for proximity detection assays
CA3182266A1 (en) 2020-06-11 2021-12-16 Vadim Lobanov Methods and systems for computational decoding of biological, chemical, and physical entities
KR102576033B1 (ko) * 2020-07-17 2023-09-11 주식회사 아론티어 3d-컨벌루션 뉴럴 네트워크의 앙상블을 이용한 단백질-리간드 결합 친화도 예측 방법 및 이를 위한 시스템
CN116685725A (zh) 2020-08-19 2023-09-01 Encodia 公司 顺序编码方法和相关试剂盒
DE112021004478T5 (de) 2020-08-25 2023-09-14 Seer, Inc. Zusammensetzungen und verfahren zur bestimmung von proteinen und nukleinsäuren
US11880345B2 (en) * 2020-09-14 2024-01-23 Tata Consultancy Services Limited Method and system for generating annotations and field-names for relational schema
EP4244382A1 (en) 2020-11-11 2023-09-20 Nautilus Subsidiary, Inc. Affinity reagents having enhanced binding and detection characteristics
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation
US20220227890A1 (en) 2021-01-21 2022-07-21 Nautilus Biotechnology, Inc. Systems and methods for biomolecule preparation
CN117413183A (zh) * 2021-03-11 2024-01-16 诺迪勒思附属公司 用于生物分子保留的系统和方法
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
CA3222270A1 (en) 2021-06-24 2022-12-29 Vadim Lobanov Methods and systems for assay refinement
EP4399528A1 (en) 2021-09-09 2024-07-17 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
EP4405683A1 (en) * 2021-09-22 2024-07-31 Nautilus Subsidiary, Inc. Methods and systems for determining polypeptide interactions
US20230114905A1 (en) 2021-10-11 2023-04-13 Nautilus Biotechnology, Inc. Highly multiplexable analysis of proteins and proteomes
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
US12474346B2 (en) 2021-11-05 2025-11-18 Encodia, Inc. Methods and kits for multicycle encoding assay
EP4441503A1 (en) 2021-11-30 2024-10-09 Nautilus Subsidiary, Inc. Particle-based isolation of proteins and other analytes
WO2023122589A2 (en) 2021-12-22 2023-06-29 Nautilus Subsidiary, Inc. Systems and methods for carrying out highly multiplexed bioanalyses
CN114388060B (zh) * 2022-01-13 2023-06-20 西湖大学 基于圆形控制器的蛋白球形截面生成方法
CN114388058A (zh) * 2022-01-13 2022-04-22 西湖大学 基于九轴imu的蛋白任意截面生成方法
CA3245990A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. INTEGRATED NETWORKS FOR SINGLE-ANALYTE PROCESSES
EP4515026A1 (en) 2022-04-25 2025-03-05 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
WO2023250364A1 (en) 2022-06-21 2023-12-28 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
US12633507B2 (en) 2022-07-29 2026-05-19 Thermo Finnigan Llc Bayesian decremental scheme for charge state deconvolution
NL2032916B1 (en) 2022-08-31 2024-03-15 Univ Delft Tech Single-molecule aptamer FRET for protein identification and structural analysis
WO2024058967A1 (en) 2022-09-13 2024-03-21 Nautilus Subsidiary, Inc. Systems and methods of validating new affinity reagents
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
EP4594519A1 (en) 2022-09-27 2025-08-06 Nautilus Subsidiary, Inc. Polypeptide capture, in situ fragmentation and identification
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
US20240183858A1 (en) 2022-11-15 2024-06-06 Nautilus Subsidiary, Inc. Standard polypeptides
US20240192202A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. Fluidic media for single-analtye arrays
EP4634285A1 (en) 2022-12-15 2025-10-22 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
US20240241110A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
US20240280568A1 (en) 2023-02-13 2024-08-22 Nautilus Subsidiary, Inc. Affinity reagents having artificial polymer scaffolds
US20240301469A1 (en) 2023-02-24 2024-09-12 Nautilus Subsidiary, Inc. Modifying, separating and detecting proteoforms
EP4689173A1 (en) 2023-03-24 2026-02-11 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
WO2024216233A1 (en) 2023-04-13 2024-10-17 Nautilus Subsidiary, Inc. Artificial proteins for displaying epitopes
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
US20240417720A1 (en) 2023-06-14 2024-12-19 Nautilus Subsidiary, Inc. Making and using structured nucleic acid particles
US20240426839A1 (en) 2023-06-21 2024-12-26 Nautilus Subsidiary, Inc. Compositions and methods for improving affinity reagent avidity
NL2035603B1 (en) 2023-08-14 2025-02-25 Univ Delft Tech Proximity labelling and FRET-based protein fingerprinting
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
US20250205706A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
US20250306015A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Single molecule-resolved characterization of affinity reagent kinetics and thermodynamics
US20250305034A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
US20250354988A1 (en) 2024-05-14 2025-11-20 Nautilus Subsidiary, Inc. Time-dependent profiling of binding interactions
WO2025255117A1 (en) 2024-06-06 2025-12-11 Nautilus Subsidiary, Inc. Analyte characterization by differential binding of binding reagents
WO2026006467A1 (en) 2024-06-27 2026-01-02 Nautilus Subsidiary, Inc. Enrichment of particle reagents for array-based assays
WO2026024964A2 (en) 2024-07-26 2026-01-29 Nautilus Subsidiary, Inc. Methods and systems for characterizing proteoform markers of parkinson's disease
US20260092914A1 (en) 2024-09-27 2026-04-02 Nautilus Subsidiary, Inc. Single analyte characterization under equilibrium binding conditions
WO2026084863A1 (en) 2024-10-17 2026-04-23 Nautilus Subsidiary, Inc. Analyte characterization via iterative analysis
US20260193640A1 (en) 2024-12-11 2026-07-09 Nautilus Subsidiary, Inc. Screening and characterization of binding reagents under equilibrium conditions

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1938430A (zh) * 2003-12-30 2007-03-28 英特尔公司 使用拉曼活性探针构建物来分析生物学样品的方法和装置

Family Cites Families (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1017524A (en) 1911-05-01 1912-02-13 Robert George Ferguson Flush-tank.
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5635602A (en) 1993-08-13 1997-06-03 The Regents Of The University Of California Design and synthesis of bispecific DNA-antibody conjugates
US6682886B1 (en) 1994-04-28 2004-01-27 Gilead Sciences, Inc. Bivalent binding molecules of 7 transmembrane G protein-coupled receptors
SE9601676D0 (sv) 1996-04-30 1996-04-30 Ulf Landegren Improved probing of specific mucleic acids
US6762056B1 (en) 1997-11-12 2004-07-13 Protiveris, Inc. Rapid method for determining potential binding sites of a protein
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US20060057573A1 (en) * 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
CA2303761C (en) 1999-04-06 2005-12-20 Micromass Limited A method of determining peptide sequences by mass spectrometry
WO2000073787A1 (en) 1999-05-27 2000-12-07 Rockefeller University An expert system for protein identification using mass spectrometric information combined with database searching
CA2393658A1 (en) 1999-12-23 2001-06-28 Illumina, Inc. Decoding of array sensors with microspheres
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
GB0022978D0 (en) * 2000-09-19 2000-11-01 Oxford Glycosciences Uk Ltd Detection of peptides
JP2005504955A (ja) * 2001-03-07 2005-02-17 バイオ−ラッド ラボラトリーズ，インコーポレイティド 多重に修飾された細胞タンパク質の同時検出及び測定の評価システム（ａｓｓａｙｓｙｓｔｅｍ）
WO2002072613A1 (en) 2001-03-10 2002-09-19 Kent Ridge Digital Labs System and method for systematic prediction of ligand/receptor activity
US20030054408A1 (en) * 2001-04-20 2003-03-20 Ramamoorthi Ravi Methods and systems for identifying proteins
US6720595B2 (en) 2001-08-06 2004-04-13 International Business Machines Corporation Three-dimensional island pixel photo-sensor
US8013134B2 (en) 2001-11-23 2011-09-06 Olink Ab Kit for proximity probing with multivalent proximity probes
AU2002351164A1 (en) 2001-11-26 2003-06-10 Molecular Reflections, Inc. Microscale immobilization of molecules using a hydrogel and methods of use thereof
US20040067599A1 (en) * 2001-12-14 2004-04-08 Katz Joseph L. Separation identification and quantitation of protein mixtures
WO2003083441A2 (en) 2002-03-29 2003-10-09 Buck Institute Identification of apoptotic peptides and methods of use thereof
US6998241B2 (en) 2002-09-11 2006-02-14 Kimberly-Clark Worldwide, Inc. Antibody pair screening methods
JP4584828B2 (ja) * 2002-10-15 2010-11-24 アブメトリックス， インコーポレイテッド 短いエピトープに対して向けられる複数組のディジタル抗体、およびその使用
TWI375796B (en) 2003-04-18 2012-11-01 Becton Dickinson Co Immuno-amplification
US20040265938A1 (en) 2003-06-25 2004-12-30 Jose Remacle Method for the determination of cell activation
JP3788513B2 (ja) * 2003-09-11 2006-06-21 セイコーエプソン株式会社 固相基板上への分子の固定化方法およびそれを用いるバイオセンサの製造方法
US7593817B2 (en) 2003-12-16 2009-09-22 Thermo Finnigan Llc Calculating confidence levels for peptide and protein identification
US20060160234A1 (en) * 2005-01-18 2006-07-20 Viorica Lopez-Avila Methods for assessing polypeptide array quality
AU2006227380B2 (en) 2005-03-17 2012-08-16 Vel Partners Holdings Llc Immunogens for vaccines against antigenically variable pathogens and diseases
EP1880206B1 (en) 2005-05-09 2012-11-14 Affymetrix, Inc. Multiplex capture of nucleic acids
EP1907410B1 (en) 2005-06-10 2012-07-04 Saint Louis University Methods for the selection of aptamers
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
DK1907571T3 (en) * 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
JP4724816B2 (ja) * 2005-09-06 2011-07-13 シャープ株式会社 タンパク質の測定方法
EP1941276A1 (de) 2005-10-29 2008-07-09 Bayer Technology Services GmbH Verfahren zur bestimmung eines oder mehrerer analyten in komplex zusammengesetzten proben biologischen ursprungs und dessen verwendung
WO2007120208A2 (en) 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
US20070218503A1 (en) 2006-02-13 2007-09-20 Mitra Robi D Methods of polypeptide identification, and compositions therefor
US7692783B2 (en) 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US8492098B2 (en) 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
WO2007117444A2 (en) 2006-03-31 2007-10-18 Yinghe Hu Protein detection by aptamers
CN100500865C (zh) 2006-04-21 2009-06-17 华东师范大学 一种通过同时识别多个表位来检测CatD蛋白的方法
US7855054B2 (en) 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US7964356B2 (en) 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
US20110136099A1 (en) 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
EP2155899A2 (en) 2007-04-10 2010-02-24 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
US8404830B2 (en) 2007-07-17 2013-03-26 Somalogic, Inc. Method for generating aptamers with improved off-rates
EP2279291B1 (en) 2008-04-09 2019-07-03 Cornell University Coferons and methods of making and using them
US9102520B2 (en) 2008-08-13 2015-08-11 California Institute Of Technology Nanocomposite structures and related methods and systems
US8222047B2 (en) 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
WO2010065531A1 (en) * 2008-12-01 2010-06-10 Robi David Mitra Single molecule protein screening
WO2010111674A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US9532956B2 (en) 2009-04-18 2017-01-03 Massachusetts Institute Of Technology PH sensitive biodegradable polymeric particles for drug delivery
US20110177107A1 (en) 2010-01-14 2011-07-21 Haplomics, Inc. Predicting and reducing alloimmunogenicity of protein therapeutics
US9678068B2 (en) 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
US8415171B2 (en) 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
NZ602618A (en) 2010-04-12 2013-12-20 Somalogic Inc Aptamers to beta-ngf and their use in treating beta-ngf mediated diseases and disorders
US8774494B2 (en) * 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
US9523680B2 (en) 2010-06-30 2016-12-20 Ambergen, Inc. Global Proteomic screening of random bead arrays using mass spectrometry imaging
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
CN102618940A (zh) 2011-01-31 2012-08-01 艾比玛特生物医药（上海）有限公司 抗体制备方法及所得抗体和抗体库
US20130053541A1 (en) 2011-03-11 2013-02-28 Lynntech, Inc. Methods for discovering molecules that bind to proteins
WO2016069124A1 (en) 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
GB2577626B (en) 2011-06-23 2020-09-23 Univ Texas Identifying peptides at the single molecule level
CA2868096C (en) 2012-03-28 2019-12-31 Somalogic, Inc. Aptamers to pdgf and vegf and their use in treating pdgf and vegf mediated conditions
NL2009191C2 (en) 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
JP6395718B2 (ja) 2012-11-19 2018-09-26 アプトン バイオシステムズ インコーポレイテッド 単一分子検出を用いた分子分析物のデジタル分析の方法
MX2015015687A (es) * 2013-05-16 2016-07-20 Basetra Medical Technology Co Ltd Diagnostico fetales que usa captura de celulas fetales de sangre materna.
US9368187B2 (en) * 2013-07-11 2016-06-14 Qualcomm Incorporated Insertion-override counter to support multiple memory refresh rates
MX361451B (es) 2013-09-24 2018-12-06 Somalogic Inc Detección objetivo de múltiples aptámeros.
CA2928207C (en) 2013-11-21 2020-09-15 Somalogic, Inc. Cytidine-5-carboxamide modified nucleotide compositions and methods related thereto
DK3087083T3 (da) 2013-12-27 2019-12-16 Hoffmann La Roche Systemisk opdagelse, modning og forlængelse af peptidbindere til proteiner
EP3009520B1 (en) 2014-10-14 2018-12-12 Karlsruher Institut für Technologie Site-specific immobilization of DNA origami structures on solid substrates
EP3274473B1 (en) 2015-03-24 2020-10-28 Pacific Biosciences of California, Inc. Methods and compositions for single molecule composition loading
US9881786B2 (en) 2015-04-02 2018-01-30 Micron Technology, Inc. Methods of forming nanostructures using self-assembled nucleic acids, and nanostructures thereof
WO2016174525A1 (en) 2015-04-29 2016-11-03 Biosims Technologies Enhanced sensitivity in ligand binding assays performed with secondary ion mass spectrometry
US11670399B2 (en) 2015-05-18 2023-06-06 The Regents Of The University Of California Systems and methods for predicting glycosylation on proteins
WO2017127762A1 (en) 2016-01-20 2017-07-27 Vitrisa Therapeutics, Inc. Methods for improved aptamer selection
KR102516168B1 (ko) 2016-05-02 2023-03-30 엔코디아, 인코포레이티드 암호화 핵산을 사용한 거대분자 분석
JP2020514746A (ja) 2016-12-01 2020-05-21 ノーティラス バイオテクノロジー インコーポレイテッド タンパク質をアッセイする方法
WO2019036055A2 (en) 2017-08-18 2019-02-21 Ignite Biosciences, Inc. METHODS OF SELECTING BINDING REAGENTS
US11125748B2 (en) 2017-09-01 2021-09-21 University Of British Columbia Method for organizing individual molecules on a patterned substrate and structures assembled thereby
US11391734B2 (en) 2017-09-25 2022-07-19 California Institute Of Technology Surface-immobilized bistable polynucleotide devices for the sensing and quantification of molecular events
CN112154230A (zh) 2017-10-23 2020-12-29 诺迪勒思生物科技公司 用于蛋白质鉴定的方法和系统
US11721412B2 (en) 2017-10-23 2023-08-08 Nautilus Subsidiary, Inc. Methods for identifying a protein in a sample of unknown proteins
WO2019089846A1 (en) 2017-10-31 2019-05-09 Encodia, Inc. Methods and compositions for polypeptide analysis
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
US20210355483A1 (en) 2017-10-31 2021-11-18 Encodia, Inc. Methods and kits using nucleic acid encoding and/or label
CA3086915A1 (en) 2017-12-29 2019-07-04 Sujal M. Patel Decoding approaches for protein identification
CN112236528A (zh) 2018-04-04 2021-01-15 诺迪勒思生物科技公司 产生纳米阵列和微阵列的方法
WO2019236749A2 (en) 2018-06-06 2019-12-12 Ignite Biosciences, Inc. Methods and applications of protein identification
JP2022513092A (ja) 2018-11-20 2022-02-07 ノーティラス・バイオテクノロジー・インコーポレイテッド 親和性試薬の設計および選択
AU2020266136B2 (en) 2019-04-29 2026-04-02 Nautilus Subsidiary, Inc. Methods and systems for integrated on-chip single-molecule detection
WO2021003470A1 (en) 2019-07-03 2021-01-07 Nautilus Biotechnology, Inc. Decoding approaches for protein and peptide identification
WO2021087402A1 (en) 2019-10-30 2021-05-06 Nautilus Biotechnology, Inc. Flow cell systems and methods
CA3182266A1 (en) 2020-06-11 2021-12-16 Vadim Lobanov Methods and systems for computational decoding of biological, chemical, and physical entities
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1938430A (zh) * 2003-12-30 2007-03-28 英特尔公司 使用拉曼活性探针构建物来分析生物学样品的方法和装置

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