JP2020505938A5 - - Google Patents
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- Publication number
- JP2020505938A5 JP2020505938A5 JP2019542675A JP2019542675A JP2020505938A5 JP 2020505938 A5 JP2020505938 A5 JP 2020505938A5 JP 2019542675 A JP2019542675 A JP 2019542675A JP 2019542675 A JP2019542675 A JP 2019542675A JP 2020505938 A5 JP2020505938 A5 JP 2020505938A5
- Authority
- JP
- Japan
- Prior art keywords
- blood
- nucleic acid
- recombinant nucleic
- gene
- demethylation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 19
- 108090000623 proteins and genes Proteins 0.000 claims 14
- 108020004707 nucleic acids Proteins 0.000 claims 11
- 150000007523 nucleic acids Chemical class 0.000 claims 11
- 102000039446 nucleic acids Human genes 0.000 claims 11
- 210000004369 blood Anatomy 0.000 claims 10
- 239000008280 blood Substances 0.000 claims 10
- 230000017858 demethylation Effects 0.000 claims 9
- 238000010520 demethylation reaction Methods 0.000 claims 9
- 210000000601 blood cell Anatomy 0.000 claims 6
- 210000002865 immune cell Anatomy 0.000 claims 5
- 108091093088 Amplicon Proteins 0.000 claims 4
- 108020004414 DNA Proteins 0.000 claims 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 4
- 210000004027 cell Anatomy 0.000 claims 4
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 2
- 238000009007 Diagnostic Kit Methods 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 2
- 210000004436 artificial bacterial chromosome Anatomy 0.000 claims 2
- 210000001106 artificial yeast chromosome Anatomy 0.000 claims 2
- 238000003556 assay Methods 0.000 claims 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 2
- 210000000688 human artificial chromosome Anatomy 0.000 claims 2
- 230000011987 methylation Effects 0.000 claims 2
- 238000007069 methylation reaction Methods 0.000 claims 2
- 210000001616 monocyte Anatomy 0.000 claims 2
- 210000005259 peripheral blood Anatomy 0.000 claims 2
- 239000011886 peripheral blood Substances 0.000 claims 2
- 229940035893 uracil Drugs 0.000 claims 2
- 108700039887 Essential Genes Proteins 0.000 claims 1
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- 210000001744 T-lymphocyte Anatomy 0.000 claims 1
- 208000007536 Thrombosis Diseases 0.000 claims 1
- 210000004507 artificial chromosome Anatomy 0.000 claims 1
- 210000003719 b-lymphocyte Anatomy 0.000 claims 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims 1
- 210000003714 granulocyte Anatomy 0.000 claims 1
- 238000000126 in silico method Methods 0.000 claims 1
- 210000000265 leukocyte Anatomy 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 210000000822 natural killer cell Anatomy 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 238000011002 quantification Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17155496.7A EP3360971A1 (en) | 2017-02-09 | 2017-02-09 | Improved method for epigenetic immune cell counting |
| EP17155496.7 | 2017-02-09 | ||
| PCT/EP2018/053206 WO2018146209A1 (en) | 2017-02-09 | 2018-02-08 | Improved method for epigenetic immune cell counting |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020505938A JP2020505938A (ja) | 2020-02-27 |
| JP2020505938A5 true JP2020505938A5 (https=) | 2021-03-18 |
Family
ID=58016595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019542675A Pending JP2020505938A (ja) | 2017-02-09 | 2018-02-08 | エピジェネティックな免疫細胞計数のための改善された方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US11319581B2 (https=) |
| EP (2) | EP3360971A1 (https=) |
| JP (1) | JP2020505938A (https=) |
| CN (1) | CN110382713A (https=) |
| AU (1) | AU2018217352A1 (https=) |
| CA (1) | CA3051314A1 (https=) |
| WO (1) | WO2018146209A1 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102018116353B4 (de) * | 2018-07-05 | 2020-06-10 | Epiontis Gmbh | Verfahren zum Nachweis und zur Zählung epigenetischer Immunzellen in humanen Blutproben für Immundiagnostik und Neugeborenen-Screening |
| EP4594522A2 (en) * | 2022-09-27 | 2025-08-06 | Guardant Health, Inc. | Methods for quantifying immune cell dna |
| CN119372296B (zh) * | 2024-12-24 | 2025-06-13 | 北京致谱医学检验实验室有限公司 | 基于基因甲基化检测实现人b淋巴细胞计数的试剂盒 |
| CN119372341B (zh) * | 2024-12-30 | 2025-04-11 | 北京致谱医学检验实验室有限公司 | 基于基因甲基化检测实现人nk淋巴细胞计数的试剂盒 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EA013634B1 (ru) * | 2005-04-15 | 2010-06-30 | Эпиджиномикс Аг | Способы и нуклеиновые кислоты для анализов клеточных пролиферативных нарушений |
| DK2199411T3 (en) * | 2008-12-16 | 2015-08-03 | Epiontis Gmbh | Epigenetic markers for the identification of CD3-positive T lymphocytes |
| US9926599B2 (en) * | 2012-03-12 | 2018-03-27 | Epiontis Gmbh | Epigenetic marker for the identification of CD3CD4 positive T lymphocytes |
| CN103031372A (zh) * | 2012-08-10 | 2013-04-10 | 深圳先进技术研究院 | Znf545基因甲基化定量检测方法 |
| GB201221133D0 (en) * | 2012-11-23 | 2013-01-09 | Epiontis Gmbh | Epigenetic method for the identification of subpopulations of CD8 and T lympocytes, in particular CD8 alpha and beta T lymphocytes |
| US10208346B2 (en) * | 2013-04-19 | 2019-02-19 | Epiontis Gmbh | Method for identifying the quantitative cellular composition in a biological sample |
-
2017
- 2017-02-09 EP EP17155496.7A patent/EP3360971A1/en active Pending
-
2018
- 2018-02-08 JP JP2019542675A patent/JP2020505938A/ja active Pending
- 2018-02-08 CN CN201880010392.6A patent/CN110382713A/zh not_active Withdrawn
- 2018-02-08 CA CA3051314A patent/CA3051314A1/en active Pending
- 2018-02-08 US US16/484,246 patent/US11319581B2/en active Active
- 2018-02-08 EP EP18702740.4A patent/EP3580358A1/en active Pending
- 2018-02-08 AU AU2018217352A patent/AU2018217352A1/en not_active Abandoned
- 2018-02-08 WO PCT/EP2018/053206 patent/WO2018146209A1/en not_active Ceased
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