CA3051314A1 - Improved method for epigenetic immune cell counting - Google Patents

Improved method for epigenetic immune cell counting Download PDF

Info

Publication number
CA3051314A1
CA3051314A1 CA3051314A CA3051314A CA3051314A1 CA 3051314 A1 CA3051314 A1 CA 3051314A1 CA 3051314 A CA3051314 A CA 3051314A CA 3051314 A CA3051314 A CA 3051314A CA 3051314 A1 CA3051314 A1 CA 3051314A1
Authority
CA
Canada
Prior art keywords
blood
cells
cell
nucleic acid
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3051314A
Other languages
English (en)
French (fr)
Inventor
Sven Olek
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precision for Medicine GmbH
Original Assignee
Epiontis GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epiontis GmbH filed Critical Epiontis GmbH
Publication of CA3051314A1 publication Critical patent/CA3051314A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3051314A 2017-02-09 2018-02-08 Improved method for epigenetic immune cell counting Pending CA3051314A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP17155496.7 2017-02-09
EP17155496.7A EP3360971A1 (en) 2017-02-09 2017-02-09 Improved method for epigenetic immune cell counting
PCT/EP2018/053206 WO2018146209A1 (en) 2017-02-09 2018-02-08 Improved method for epigenetic immune cell counting

Publications (1)

Publication Number Publication Date
CA3051314A1 true CA3051314A1 (en) 2018-08-16

Family

ID=58016595

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3051314A Pending CA3051314A1 (en) 2017-02-09 2018-02-08 Improved method for epigenetic immune cell counting

Country Status (7)

Country Link
US (1) US11319581B2 (https=)
EP (2) EP3360971A1 (https=)
JP (1) JP2020505938A (https=)
CN (1) CN110382713A (https=)
AU (1) AU2018217352A1 (https=)
CA (1) CA3051314A1 (https=)
WO (1) WO2018146209A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102018116353B4 (de) * 2018-07-05 2020-06-10 Epiontis Gmbh Verfahren zum Nachweis und zur Zählung epigenetischer Immunzellen in humanen Blutproben für Immundiagnostik und Neugeborenen-Screening
WO2024073508A2 (en) * 2022-09-27 2024-04-04 Guardant Health, Inc. Methods and compositions for quantifying immune cell dna
CN119372296B (zh) * 2024-12-24 2025-06-13 北京致谱医学检验实验室有限公司 基于基因甲基化检测实现人b淋巴细胞计数的试剂盒
CN119372341B (zh) * 2024-12-30 2025-04-11 北京致谱医学检验实验室有限公司 基于基因甲基化检测实现人nk淋巴细胞计数的试剂盒

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130021400A (ko) * 2005-04-15 2013-03-05 에피제노믹스 아게 세포 증식 질환을 분석하기 위한 방법 및 핵산
DK2199411T3 (en) * 2008-12-16 2015-08-03 Epiontis Gmbh Epigenetic markers for the identification of CD3-positive T lymphocytes
WO2013135454A1 (en) * 2012-03-12 2013-09-19 Epiontis Gmbh Epigenetic marker for the identification of cd3cd4 positive t lymphocytes
CN103031372A (zh) * 2012-08-10 2013-04-10 深圳先进技术研究院 Znf545基因甲基化定量检测方法
GB201221133D0 (en) * 2012-11-23 2013-01-09 Epiontis Gmbh Epigenetic method for the identification of subpopulations of CD8 and T lympocytes, in particular CD8 alpha and beta T lymphocytes
JP6590790B2 (ja) * 2013-04-19 2019-10-16 エピオンティス ゲーエムベーハー 生体サンプルにおける定量的細胞組成を特定する方法

Also Published As

Publication number Publication date
WO2018146209A1 (en) 2018-08-16
CN110382713A (zh) 2019-10-25
US20190390263A1 (en) 2019-12-26
US11319581B2 (en) 2022-05-03
EP3360971A1 (en) 2018-08-15
AU2018217352A1 (en) 2019-08-08
EP3580358A1 (en) 2019-12-18
JP2020505938A (ja) 2020-02-27

Similar Documents

Publication Publication Date Title
Baron et al. Epigenetic immune cell counting in human blood samples for immunodiagnostics
Angulo et al. A comparison of EGFR mutation testing methods in lung carcinoma: direct sequencing, real-time PCR and immunohistochemistry
Rasmussen et al. Quantitation of minimal residual disease in multiple myeloma using an allele-specific real-time PCR assay
US11319581B2 (en) Method for epigenetic immune cell counting
CA2850912C (en) Fetal chromosomal aneuploidy diagnosis
JP2019162102A (ja) 末梢血中で、がんによって変化したｒｎａを検出するシステムおよび方法
Herreros-Villanueva et al. KRAS mutations: analytical considerations
Guo et al. Detection of plasma EGFR mutations in NSCLC patients with a validated ddPCR lung cfDNA assay
WO2014170497A2 (en) Method for identifying the quantitative cellular composition in a biological sample
CN107002131B (zh) 作为确认骨髓增生异常综合征诊断的可靠测试的外周血血浆dna深度测序
Liu et al. Laboratory-developed droplet digital PCR assay for quantification of the JAK2V617F mutation
US10273545B2 (en) Epigenetic method for the identification of subpopulations of CD8+ T lymphocytes, in particular CD8 alpha and beta T lymphocytes
US12006546B2 (en) Method for epigenetic immune cell detection and counting in human blood samples
McCafferty et al. A novel next-generation sequencing capture-based strategy to report somatic hypermutation status using genomic regions downstream to immunoglobulin rearrangements
HK1259669A1 (en) Improved method for epigenetic immune cell counting
EP3818179B1 (en) Epigenetic method to detect and distinguish ipex and ipex-like syndromes, in particular in newborns
EP4269614A1 (en) Method for detecting copy number of specific nucleic acid per single cell
Li et al. The 1357 bp deletion in β-thalassemia: molecular profiling and hematological characterization in a Guangxi cohort
CN119242823B (zh) 基于基因甲基化检测实现人t淋巴细胞计数的试剂盒
CN119372341B (zh) 基于基因甲基化检测实现人nk淋巴细胞计数的试剂盒
Garza et al. Development of a high-resolution melting curve analysis screening test for SRSF2 splicing factor gene mutations in myelodysplastic syndromes
Cai et al. P731: TERT gene promoter region mutation analysis in glioma using multiplex SNaPshot method
MAWLOOD et al. Methylation status and human age at three autosomal loci: a new forensic profiling tool
Douzandegan et al. Development of a LAMP-based diagnostic method for HTLV-1 using Iranian clinical samples
Alves et al. Genomic diversity and BCL9L mutational status in CTC pools predict overall survival in metastatic colorectal cancer

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20220301

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION

Effective date: 20241001

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20250115

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 7TH ANNIV.) - STANDARD

Year of fee payment: 7

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250122

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20250122

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250122

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT

Effective date: 20250518

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED

Effective date: 20250518

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20250710

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION

Effective date: 20251105

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT

Effective date: 20251231

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED

Effective date: 20251231

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20251231

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 8TH ANNIV.) - STANDARD

Year of fee payment: 8

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20260126

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20260128