CN110382713A - 改进的表观遗传免疫细胞计数方法 - Google Patents

改进的表观遗传免疫细胞计数方法 Download PDF

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Publication number
CN110382713A
CN110382713A CN201880010392.6A CN201880010392A CN110382713A CN 110382713 A CN110382713 A CN 110382713A CN 201880010392 A CN201880010392 A CN 201880010392A CN 110382713 A CN110382713 A CN 110382713A
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China
Prior art keywords
blood
cells
nucleic acid
demethylation
cell
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CN201880010392.6A
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English (en)
Chinese (zh)
Inventor
斯文·欧莱克
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Precision for Medicine GmbH
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Epiontis GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201880010392.6A 2017-02-09 2018-02-08 改进的表观遗传免疫细胞计数方法 Withdrawn CN110382713A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP17155496.7 2017-02-09
EP17155496.7A EP3360971A1 (en) 2017-02-09 2017-02-09 Improved method for epigenetic immune cell counting
PCT/EP2018/053206 WO2018146209A1 (en) 2017-02-09 2018-02-08 Improved method for epigenetic immune cell counting

Publications (1)

Publication Number Publication Date
CN110382713A true CN110382713A (zh) 2019-10-25

Family

ID=58016595

Family Applications (1)

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CN201880010392.6A Withdrawn CN110382713A (zh) 2017-02-09 2018-02-08 改进的表观遗传免疫细胞计数方法

Country Status (7)

Country Link
US (1) US11319581B2 (https=)
EP (2) EP3360971A1 (https=)
JP (1) JP2020505938A (https=)
CN (1) CN110382713A (https=)
AU (1) AU2018217352A1 (https=)
CA (1) CA3051314A1 (https=)
WO (1) WO2018146209A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102018116353B4 (de) * 2018-07-05 2020-06-10 Epiontis Gmbh Verfahren zum Nachweis und zur Zählung epigenetischer Immunzellen in humanen Blutproben für Immundiagnostik und Neugeborenen-Screening
WO2024073508A2 (en) * 2022-09-27 2024-04-04 Guardant Health, Inc. Methods and compositions for quantifying immune cell dna
CN119372296B (zh) * 2024-12-24 2025-06-13 北京致谱医学检验实验室有限公司 基于基因甲基化检测实现人b淋巴细胞计数的试剂盒
CN119372341B (zh) * 2024-12-30 2025-04-11 北京致谱医学检验实验室有限公司 基于基因甲基化检测实现人nk淋巴细胞计数的试剂盒

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2199411A1 (en) * 2008-12-16 2010-06-23 Epiontis GmbH Epigenetic markers for the identification of CD3 positive T-lymphocytes
CN103031372A (zh) * 2012-08-10 2013-04-10 深圳先进技术研究院 Znf545基因甲基化定量检测方法
CN104046686A (zh) * 2005-04-15 2014-09-17 Epi基因组股份公司 分析细胞增殖性病症的方法和核酸
WO2014170497A2 (en) * 2013-04-19 2014-10-23 Epiontis Gmbh Method for identifying the quantitative cellular composition in a biological sample

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013135454A1 (en) * 2012-03-12 2013-09-19 Epiontis Gmbh Epigenetic marker for the identification of cd3cd4 positive t lymphocytes
GB201221133D0 (en) * 2012-11-23 2013-01-09 Epiontis Gmbh Epigenetic method for the identification of subpopulations of CD8 and T lympocytes, in particular CD8 alpha and beta T lymphocytes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104046686A (zh) * 2005-04-15 2014-09-17 Epi基因组股份公司 分析细胞增殖性病症的方法和核酸
EP2199411A1 (en) * 2008-12-16 2010-06-23 Epiontis GmbH Epigenetic markers for the identification of CD3 positive T-lymphocytes
CN103031372A (zh) * 2012-08-10 2013-04-10 深圳先进技术研究院 Znf545基因甲基化定量检测方法
WO2014170497A2 (en) * 2013-04-19 2014-10-23 Epiontis Gmbh Method for identifying the quantitative cellular composition in a biological sample

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JALID SEHOULI等: "Epigenetic quantification of tumor-infiltrating T-lymphocytes", 《EPIGENETICS》 *
李一君: "自身免疫糖尿病外周血CD4+T细胞DNA甲基化异常的研究", 《中国博士学位论文全文数据库 医药卫生科技辑》 *

Also Published As

Publication number Publication date
WO2018146209A1 (en) 2018-08-16
CA3051314A1 (en) 2018-08-16
US20190390263A1 (en) 2019-12-26
US11319581B2 (en) 2022-05-03
EP3360971A1 (en) 2018-08-15
AU2018217352A1 (en) 2019-08-08
EP3580358A1 (en) 2019-12-18
JP2020505938A (ja) 2020-02-27

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Application publication date: 20191025

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