JP2020000259A5 - - Google Patents
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- Publication number
- JP2020000259A5 JP2020000259A5 JP2019187777A JP2019187777A JP2020000259A5 JP 2020000259 A5 JP2020000259 A5 JP 2020000259A5 JP 2019187777 A JP2019187777 A JP 2019187777A JP 2019187777 A JP2019187777 A JP 2019187777A JP 2020000259 A5 JP2020000259 A5 JP 2020000259A5
- Authority
- JP
- Japan
- Prior art keywords
- cbs
- protein
- chromatography
- column
- containing solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims 21
- 102000004169 proteins and genes Human genes 0.000 claims 16
- 108090000623 proteins and genes Proteins 0.000 claims 16
- 102100021935 C-C motif chemokine 26 Human genes 0.000 claims 5
- 101000897493 Homo sapiens C-C motif chemokine 26 Proteins 0.000 claims 5
- 210000004027 cell Anatomy 0.000 claims 5
- 239000011347 resin Substances 0.000 claims 5
- 229920005989 resin Polymers 0.000 claims 5
- 238000013375 chromatographic separation Methods 0.000 claims 4
- 238000004587 chromatography analysis Methods 0.000 claims 4
- 238000004255 ion exchange chromatography Methods 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 3
- 238000001042 affinity chromatography Methods 0.000 claims 3
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims 3
- 239000002184 metal Substances 0.000 claims 3
- 229910052751 metal Inorganic materials 0.000 claims 3
- 150000007523 nucleic acids Chemical group 0.000 claims 3
- 238000000926 separation method Methods 0.000 claims 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims 2
- 229920005654 Sephadex Polymers 0.000 claims 2
- 239000012507 Sephadex™ Substances 0.000 claims 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 150000001450 anions Chemical class 0.000 claims 2
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 2
- 229910052725 zinc Inorganic materials 0.000 claims 2
- 239000011701 zinc Substances 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 1
- 102100034976 Cystathionine beta-synthase Human genes 0.000 claims 1
- 108010073644 Cystathionine beta-synthase Proteins 0.000 claims 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 102000016943 Muramidase Human genes 0.000 claims 1
- 108010014251 Muramidase Proteins 0.000 claims 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 210000004899 c-terminal region Anatomy 0.000 claims 1
- 239000000919 ceramic Substances 0.000 claims 1
- 239000012504 chromatography matrix Substances 0.000 claims 1
- 229910017052 cobalt Inorganic materials 0.000 claims 1
- 239000010941 cobalt Substances 0.000 claims 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims 1
- 229910052802 copper Inorganic materials 0.000 claims 1
- 239000010949 copper Substances 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 239000003937 drug carrier Substances 0.000 claims 1
- 239000012149 elution buffer Substances 0.000 claims 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 229960000274 lysozyme Drugs 0.000 claims 1
- 235000010335 lysozyme Nutrition 0.000 claims 1
- 239000004325 lysozyme Substances 0.000 claims 1
- 229910021645 metal ion Inorganic materials 0.000 claims 1
- 229910052759 nickel Inorganic materials 0.000 claims 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims 1
- 230000003094 perturbing effect Effects 0.000 claims 1
- 239000008194 pharmaceutical composition Substances 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000000527 sonication Methods 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261615629P | 2012-03-26 | 2012-03-26 | |
| US61/615,629 | 2012-03-26 | ||
| US13/830,494 US9243239B2 (en) | 2012-03-26 | 2013-03-14 | Purification of cystathionine beta-synthase |
| US13/830,494 | 2013-03-14 | ||
| JP2017078808A JP6839595B2 (ja) | 2012-03-26 | 2017-04-12 | シスタチオンβ−シンターゼの精製 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017078808A Division JP6839595B2 (ja) | 2012-03-26 | 2017-04-12 | シスタチオンβ−シンターゼの精製 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020000259A JP2020000259A (ja) | 2020-01-09 |
| JP2020000259A5 true JP2020000259A5 (https=) | 2020-03-19 |
| JP6952361B2 JP6952361B2 (ja) | 2021-10-20 |
Family
ID=49212017
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015503433A Active JP6146934B2 (ja) | 2012-03-26 | 2013-03-25 | シスタチオンβ−シンターゼの精製 |
| JP2017078808A Active JP6839595B2 (ja) | 2012-03-26 | 2017-04-12 | シスタチオンβ−シンターゼの精製 |
| JP2019187777A Active JP6952361B2 (ja) | 2012-03-26 | 2019-10-11 | シスタチオンβ−シンターゼの精製 |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015503433A Active JP6146934B2 (ja) | 2012-03-26 | 2013-03-25 | シスタチオンβ−シンターゼの精製 |
| JP2017078808A Active JP6839595B2 (ja) | 2012-03-26 | 2017-04-12 | シスタチオンβ−シンターゼの精製 |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US9243239B2 (https=) |
| EP (3) | EP3263701B1 (https=) |
| JP (3) | JP6146934B2 (https=) |
| CN (1) | CN104540940B (https=) |
| AU (1) | AU2013240003B2 (https=) |
| BR (1) | BR112014023570B1 (https=) |
| CA (1) | CA2867719C (https=) |
| HK (1) | HK1246350B (https=) |
| IL (2) | IL234635B (https=) |
| IN (1) | IN2014DN07920A (https=) |
| WO (1) | WO2013148580A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9034318B2 (en) | 2011-08-30 | 2015-05-19 | The Regents Of The University Of Colorado, A Body Corporate | Chemically modified cystathionine beta-synthase enzyme for treatment of homocystinuria |
| US9243239B2 (en) * | 2012-03-26 | 2016-01-26 | The Regents Of The University Of Colorado, A Body Corporate | Purification of cystathionine beta-synthase |
| US9675678B2 (en) | 2013-01-29 | 2017-06-13 | The Regents Of The University Of Colorado, A Body Corporate | Compositions and methods for treatment of homocystinuria |
| FI3998067T3 (fi) * | 2015-11-09 | 2024-10-31 | Univ Colorado Regents | Koostumukset ja menetelmät homokystinurian hoitoon |
| IL269544B2 (en) | 2017-04-17 | 2025-01-01 | Univ Colorado Regents | Streamlining enzyme replacement therapy for the treatment of high homocysteine |
| US20220315965A1 (en) * | 2019-06-25 | 2022-10-06 | Zymergen Inc. | Engineered biosynthetic pathways for production of cystathionine by fermentation |
| ES3058596T3 (en) * | 2019-06-26 | 2026-03-11 | Travere Therapeutics Switzerland Gmbh | Pegylated cystathionine beta synthase for enzyme therapy for treatment of homocystinuria |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5576559A (en) | 1978-12-01 | 1980-06-09 | Chiyou Lsi Gijutsu Kenkyu Kumiai | Electron beam fine adjusting device |
| US5523225A (en) | 1993-09-13 | 1996-06-04 | Regents Of The University Of Colorado | DNA sequence encoding human cystathionine β-synthase |
| US5635375A (en) | 1995-01-09 | 1997-06-03 | Regents Of The University Of Colorado | Method of increasing the yield and heme saturation of cystathione β-synthase |
| AU2003251550A1 (en) | 2002-06-17 | 2003-12-31 | The Regents Of The University Of Colorado | HUMAN CYSTATHIONINE Beta-SYNTHASE VARIANTS AND METHODS OF PRODU CTION THEREOF |
| GB0216001D0 (en) * | 2002-07-10 | 2002-08-21 | Nat Blood Authority | Process and composition |
| EP1396537A1 (en) | 2002-09-04 | 2004-03-10 | Avidis SA | Treatment of elevated plasma homocysteine |
| CN1552905A (zh) * | 2003-12-18 | 2004-12-08 | 中国农业科学院茶叶研究所 | 一类茶树胱硫醚-γ-合成酶特异表达序列标签及其生物芯片 |
| EP1878739A1 (en) | 2006-07-14 | 2008-01-16 | LEK Pharmaceuticals D.D. | One step IMAC (MCAC) purification of proteins |
| CA2788689C (en) | 2010-02-04 | 2019-07-02 | The Board Of Regents Of The University Of Texas System | Engineered enzymes with methionine-gamma-lyase enzymes and pharmacological preparations thereof |
| US9243239B2 (en) | 2012-03-26 | 2016-01-26 | The Regents Of The University Of Colorado, A Body Corporate | Purification of cystathionine beta-synthase |
-
2013
- 2013-03-14 US US13/830,494 patent/US9243239B2/en active Active
- 2013-03-25 EP EP17165825.5A patent/EP3263701B1/en active Active
- 2013-03-25 EP EP19179948.5A patent/EP3569706A1/en not_active Withdrawn
- 2013-03-25 WO PCT/US2013/033716 patent/WO2013148580A1/en not_active Ceased
- 2013-03-25 JP JP2015503433A patent/JP6146934B2/ja active Active
- 2013-03-25 EP EP13716577.5A patent/EP2831229B8/en active Active
- 2013-03-25 AU AU2013240003A patent/AU2013240003B2/en active Active
- 2013-03-25 CA CA2867719A patent/CA2867719C/en active Active
- 2013-03-25 CN CN201380027463.0A patent/CN104540940B/zh active Active
- 2013-03-25 BR BR112014023570-8A patent/BR112014023570B1/pt active IP Right Grant
- 2013-03-25 IN IN7920DEN2014 patent/IN2014DN07920A/en unknown
-
2014
- 2014-09-14 IL IL234635A patent/IL234635B/en active IP Right Grant
-
2015
- 2015-12-16 US US14/970,814 patent/US10160962B2/en active Active
-
2017
- 2017-04-12 JP JP2017078808A patent/JP6839595B2/ja active Active
-
2018
- 2018-05-03 HK HK18105730.4A patent/HK1246350B/en unknown
- 2018-11-01 US US16/178,024 patent/US20190055537A1/en not_active Abandoned
- 2018-11-21 IL IL26316218A patent/IL263162B/en active IP Right Grant
-
2019
- 2019-10-11 JP JP2019187777A patent/JP6952361B2/ja active Active
-
2020
- 2020-01-10 US US16/739,169 patent/US10941392B2/en active Active
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