JP2020000037A - Mushroom cultivation method by extension film - Google Patents

Abstract

To provide a mushroom cultivation method capable of efficiently utilizing a cultivation chamber room, and securing generation of mushroom preferably.SOLUTION: A mushroom cultivation method uses a cultivation container 1 comprising: a container body 2 in which a medium 3 can be filled; a combined film body C formed by sticking a film A comprising extension property and light transmissivity and a film B comprising vent holes, and pores which transmit oxygen and do not transmit bacterial cells; and fastening means 4. The method comprises: a mycelium cultivation preceding period step for spreading inoculated mycelium to the medium 3 under the combined film body C comprising extension property, oxygen transmissivity and light transmissivity; and a mycelium cultivation latter period step for, when an anlage formation is started by mycelium spreading, and the medium 3 is protruded due to formation of a mycelial pellet, promoting the anlage formation so that the combined film C comprising extension property follows protrusion, and increase of an amount of mycelium is not interfered.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a method for cultivating a mushroom utilizing a film body C formed by combining a film A having spreadability and translucency with a film B having pores that allow oxygen to pass but do not allow bacteria to pass.

Mushroom cultivation methods are roughly classified into a method in which the medium is packed in a closed bottle and cultivation (bottle cultivation) and a method in which the medium is cultivated in a bag made of polyethylene or the like (bag cultivation). I have.
In the bottle cultivation, the bottles can be stacked on the shelves in the cultivation room, or the height of the shelves can be shortened, and the density can be increased and stored efficiently in the room. On the other hand, since it is closed with a hard cap, the bulge of the culture medium is crushed and the formation of the mushroom primordium is inhibited, and when the cap is removed, the cap material and the mushroom adhere to each other, damaging the mushroom, etc. Has the disadvantage of the occurrence of
On the other hand, in the case of bag cultivation, a space for promoting primordium formation and growth of fruiting bodies can be provided in the upper part, and good generation conditions are provided. On the other hand, a space for generation arranged in the upper part of the bag. However, they have a drawback that they hinder storage in a room and lack storage density.

  In addition, in both bottle cultivation and bag cultivation, a gap is formed between the bottle body and the lid, or a non-woven fabric is used in order to take in the air required for primordium formation and growth of fruiting bodies. However, in both cases, the amount of air supplied to the generating surface tends to be non-uniform, which is one of the causes of producing a bias in the mushroom generation site.

  Further, light irradiation is required for the formation of the mushroom primordia, but the illuminance after passing through the material is generally reduced to about 83% since a translucent material is used. And delays the culture period.

  In addition, patent document 1 exists as a technique with respect to the production | generation of the mycelium mass with respect to a mushroom culture medium. However, this relates to a method of inoculating a cultivation medium in which a mycelial mass is unlikely to be generated during production or storage, and has a different purpose from the present invention which does not inhibit the growth of a mycelial mass.

JP 2006-280371 A

  The present invention has been made in view of the above circumstances, and is a film body formed by combining a film A having extensibility and translucency with a film B having pores that allow oxygen to pass but do not allow bacteria to pass. C provides a high storage density that efficiently utilizes the cultivation room space and a compatibility that ensures good mushroom generation conditions, and a uniform, evenly distributed site due to the oxygen permeability and translucency of the film. It is intended to shorten the cultivation period in addition to promoting primordium formation, and to provide an economical method for cultivating mushrooms by using a relatively inexpensive film.

  In order to solve the above-mentioned problems, a method for growing a mushroom using a stretchable film according to claim 1 comprises a container main body having shape-retaining properties, an opening provided on an upper surface, which can be filled with a certain amount of medium, and oxygen. Ventilation holes a are formed in a film A having extensibility and translucency without requiring transparency, and a film B having pores that allow oxygen to pass through but does not allow bacteria to pass through the ventilation holes a. Using a cultivation container provided with a combined film body C made to adhere and a fixing means for fixing the film body to the container body, a) Filling the container body with a sterilized medium, or filling the medium. A sterilization / filling step of a culture medium in which the container body is sterilized later; and b) a seed culture inoculation step in which the seed medium is sprayed on the surface of the culture medium, and the upper surface of the container body is covered with the above-mentioned combined film body C and tightly fixed by fixing means. And c) in an oxygen permeable, translucent environment, A mycelium cultivation pre-process for spreading the seeded mycelia in the medium, and d) a combination having extensibility in the protuberance when the formation of the primordium is initiated by the spread of the mycelia and the medium is raised by the formation of a hyphal mass. A late stage of mycelial culture in which the film body C follows and promotes primordium formation without hindering the increase in the amount of mycelium, and e) after primordium formation is completed, the fruit body is removed with the film body removed from the container body. A method for cultivating a mushroom using an extensible film, comprising: a fruiting body growing step for promoting growth; and f) a collecting step for collecting a mature fruiting body.

  In the method for cultivating a mushroom using the stretchable film according to claim 2, the step of filling the culture medium is performed such that the culture medium is flattened on the upper surface of the container or is filled at least to a height within 20 mm from the top of the container. It is characterized by having done.

  The cultivation method of mushrooms using the extensible film according to the third aspect is characterized in that the combination film body C has a smooth surface that does not have adhesion to the mushrooms.

  According to a fourth aspect of the present invention, there is provided a method for cultivating a mushroom using an extensible film, wherein a lighting device is disposed on an upper portion of a container body.

In the cultivation method according to the first aspect of the present invention, the supply of oxygen is required during the first stage of culture in which the inoculated mycelium spreads in the medium and the second stage of culture in which primordium formation proceeds. In the combination film body C of the present invention, since the film A having the pores through which oxygen can pass but the bacteria do not pass through is attached to the film A in which the vent holes a are formed, the vent holes a and Sufficient oxygen is supplied from the film B adhered thereto, and the oxygen is supplied uniformly and evenly to the entire medium.
Further, since the film B also has pores that do not allow passage of bacterial cells, the film B does not allow bacterial cells such as bacterial cells to enter, and thus acts as a defense against bacterial cells.

Further, in the formation of the primordium, light irradiation is required. In the combination film C of the present invention, the film A having extensibility and translucency allows oxygen to pass therethrough. The film B having pores that do not allow the passage of bacterial cells gives a light stimulus to the culture medium, progresses from vegetative growth to reproductive growth, and can promote proper primordium formation.
At the same time, by the combination film C having the same light-transmitting property, sufficient light is administered to the medium at the time of formation of the primordium, proper primordium formation is promoted, and the cultivation period can be shortened. .

  In the case where the primordium formation is continued and the medium rises due to the mycelial mass generated on the surface of the medium, the combined film C of the present invention has a film A having spreadability. The film body can be extended following the bulge of the medium, and the mycelial mass can be suppressed and the adverse effect of preventing an increase in the amount of mycelium can be avoided.

  When the mycelium has been sufficiently increased and the primordium formation has been completed, such as the appearance of browning on the surface of the culture medium, the film body is removed from the container body and placed in a generation chamber at about 15 ° C. Can promote growth.

  The formation of primordia is promoted under a sufficient amount of mycelium, and the fruiting bodies grown under the appropriate environment can secure the appropriate size and number. Can be obtained.

At this time, according to the cultivation container used in the present invention, the height of the shelf can be shortened or stacked on the shelf of the cultivation room, and the storage density can be increased by installing a large number in the cultivation room. As described above, at the same time, as described above, when the culture medium rises, the film A having extensibility in the combined film body C can be extended following the rise of the culture medium, and the mycelial mass is suppressed. Can be overcome.
Therefore, it is possible to satisfy both requirements of a high storage density and ensuring good conditions for generating mushrooms.

  As described above, the combined film body C has both the properties of extensibility and translucency on the one hand and oxygen permeability on the other hand by combining the two films A and B, and It can be realized economically with relatively inexpensive films.

  In the cultivation method of claim 2, the step of filling the medium is performed by filling the medium with a flat top of the container or by filling at least a height within 20 mm from the top of the container. The space between the film and the film can be reduced to zero or minimal, and it is possible to prevent the respiration of mushrooms by carbon dioxide and prevent the water content of the culture medium from decreasing.

  According to the cultivation method of the fourth aspect, by making the combination film body a smooth surface having no adhesion to the mushroom, the possibility of damaging the mushroom at the time of growth of the fruit body or the like is eliminated.

  According to the cultivation method of the fifth aspect, by arranging the lighting device on the upper portion of the container body, the formation of primordia can be promoted under sufficient light irradiation.

It is a perspective view showing the cultivation container of the present invention. In the plan view showing the combination film body C of the present invention, (a) shows a case where relatively large air holes are formed, and (b) shows a case where small air holes are scattered. XX sectional view of FIG. It is a chart figure showing the flow of each process of the present invention. It is a typical sectional view of each process of the present invention. It is sectional drawing which shows the state cultivated using the cultivation container of this invention, (A) shows the case where it piles up, and (B) shows the case where it mounts on a shelf. It is a photograph of the shiitake mushroom obtained in the Example. It is a schematic perspective view which shows the conventional cultivation container, (a) shows the case of bottle cultivation, (b) shows the case of bag cultivation.

The mushrooms applicable to the present invention include mushrooms that can be cultivated in containers such as shiitake mushroom, nameko, bunashimeji, enokitake mushroom, and eryngii.
As the medium 3, hardwood sawdust, corncob, cottonseed scum, conifers, and the like can be used.

As shown in FIG. 1, a cultivation container 1 of the present invention uses a container main body 2 having shape retention and capable of being filled with a certain amount of culture medium 3.
The shape-retaining property is intended to exclude a bag body that may be deformed, and as shown in FIG. 6, refers to a material having rigidity that can be stacked when the legs 2a are attached. For example, plastic such as polypropylene is used. No.
This container functions as a cultivation container, and is capable of being filled with the above-mentioned constant volume of medium, and has an opening at an upper portion as necessary for inoculation, spread of hyphae, formation of primordia, etc. to be described later. .

Next, according to the present invention, a vent hole a is formed in the film A having extensibility and light transmissivity without requiring oxygen permeability, and a fine hole which allows oxygen to pass through but does not pass bacteria. A combined film body C obtained by attaching a film B having holes is used.
Here, the extensibility refers to the ability to stretch following the bulge of the culture medium due to the formation of a mycelium mass described below.
Oxygen permeability refers to the ability to pass oxygen required for the spread of mycelia and the formation of primordia.
The light-transmitting property refers to a performance that enables light necessary for the spread of mycelia and the formation of the primordia to be collected from natural light or the lighting device 5 as described above.
The sticking means that the film A and the film B are bonded to each other so that the film A and the film B are brought into close contact with each other and gas is not allowed to pass through, and the means may be adhesion, adhesion, welding or the like.

In the above-mentioned film A, to have extensibility and translucency without requiring oxygen permeability, it is essential to have extensibility and translucency, but it is not necessary to have oxygen permeability. It means that it is not a necessary requirement.
Examples of such a film include a film made of polyvinyl chloride and a film made of polyethylene. Specifically, Hitachi Wrap (trade name of Hitachi Chemical Co., Ltd.) is suitable and has a thickness of 8 μm, a transparency of 98% or more, an MD of about 200%, and a TD of about 300%.

In addition, the film B having pores that allow oxygen to pass through but does not allow cells to pass through is provided with a kind of filter effect that allows oxygen to pass but does not allow cells of a larger diameter to pass through due to the diameter of the pores. The film is capable of supplying oxygen but has a function of preventing invasion of various bacteria and the like.
As a film satisfying this function, for example, TRUSCO's breathable pressure-sensitive adhesive tape TBAT-252 (trade name of Trusco Nakayama Co., Ltd.) can be cited, and has a thickness of 0.15 mm and a breathability of 170 cc / cm ² · sec. Is shown.

The combined film body C is provided with fixing means 4 for covering the upper surface of the container main body 2 and fixing the same to the upper surface. Specifically, first, when the upper outer edge of the container body is formed into a smooth surface and the film body C is brought into close contact with the outer edge, the film body C is fixed to the container body by a vacuum adhesion action. There are means. Secondly, there is a means for covering the upper edge portion of the container body with the film body C and tying the film body C with a rubber string, a tape, a thread string or the like. A third example is a device that can tighten the vicinity of the upper edge portion via a spring body.
In any case, it is sufficient that the combined film body C does not come off from the container main body 2 when the above-mentioned combined film body C is subjected to a bulging action by a mycelium mass, which will be described later.

  In the present invention, using such a cultivation container 1, as shown in FIG. 6, a sterilization / filling step of a culture medium, an inoculum inoculation step, a first step of mycelium culture, a late step of mycelium culture, a fruiting body growing step, and a collecting step Shall be applied.

First, in the sterilization / filling step, there is a method of filling the container body 2 with the sterilized culture medium 3, and more specifically, the entire culture medium 3 is steam-sterilized.
Alternatively, there is a method in which the culture medium 3 is filled in the container main body 2, the film body C is once attached to the edge, and then sterilization is performed. Specifically, the edge of the container body 2 filled with the culture medium 3 is covered with the combination film C, and steam sterilization is performed at 120 ° C. for 60 minutes.
Which is better is determined individually, but when the scale is relatively large, the former is suitable, and when the scale is small, the latter is suitable.

In this filling step, the most desirable form is to fill the culture medium 3 up to the upper edge of the container body 2 and make the surface thereof smooth and flat as shown in FIG.
The reason is that when the combination film C is put on the culture medium in a later step, the combination film C and the culture medium are brought into close contact with each other, and the gap space can be minimized. If there is a large interstitial space, the generated carbon dioxide gas stays in the space due to its specific gravity and hinders the respiration of mushrooms, and the space where water can evaporate from the surface of the culture medium becomes large, and the surface of the culture medium becomes large. This is because the water content of the water decreases.

If it does not reach a flat shape, it is desirable that the space be within at least 20 mm from the top end of the container body.
This is because, similarly to the above, by suppressing the pool space to a certain limit, it is possible to minimize the hindrance of respiration to mushrooms and the reduction of surface moisture.

After the container body is filled with the culture medium, an inoculation step of inoculating the surface of the culture medium with a seed fungus of a mushroom to be cultivated is performed.
When the combination film body C is covered in the filling step, the combination film body C is removed and inoculated.
The inoculation is performed in the same manner as inoculation of a normal inoculum, for example, by making the inoculum of fine particles into a suspension with water, and spraying the suspension down (see FIG. 5B).
Then, the container body 2 is covered with the combination film body C, and is tightly fixed by the fixing means 4 (see FIG. 5C).
As described above, the fixing means 4 forms the upper outer edge of the container body 2 on a smooth surface, or binds the upper outer edge with a rubber cord or tape, a thread cord or the like to the combined film body C by a mycelium mass described later. When a bulging action is applied, the combined film body C is kept from being adhered without coming off the container body.
The fixation of the combination film body C is for preventing contamination due to invasion of various bacteria and for normal hyphal growth.

When the inoculation is completed and a certain culturing period has elapsed, the inoculum spreads in the medium, and the first culturing step is performed (see FIG. 5 (d)).
Oxygen supply is essentially required for the spread of mycelia and the formation of the primordium described below.
However, as shown in FIG. 7, in the conventional intake of oxygen, a gap is formed between the bottle body and the lid body, but the supply amount of oxygen to the generation surface becomes non-uniform, and the mushroom generation site is biased. Is one of the causes of
On the other hand, the combined film body C of the present invention is formed by adhering a film B having pores that allow oxygen to pass through but does not allow bacteria to pass through the ventilation holes a formed in the film A. A sufficient oxygen supply is made from a and the film B stuck thereon.
That is, first, since there is a possibility that the supply of necessary oxygen may not be satisfied in the film A which does not require oxygen permeability, a vent hole a is formed there, and a pore through which oxygen is passed is formed in the opened hole. The provided film B is adhered.
For example, as shown in FIG. 2 (a), this vent hole a has a case where several relatively large holes having a diameter of about 5 to 10 mm are formed in a film body of 10 cm × 10 cm, and FIG. As shown in ()), relatively small holes having a diameter of about 0.5 to 2 mm may be dispersed in the film body.
Which one is to be selected depends on the type of mushroom and the like.
The shape is a round shape as a standard, but may be a square shape, a rhombus, or the like.
On the other hand, since the film B is also provided with pores that do not allow passage of bacterial cells, it does not allow bacterial cells such as various bacteria to enter, and can perform the function of protecting various bacterial cells.
The sticking state is as shown in the cross-sectional view of FIG. 3, and the air holes a formed in the film A are closed with the film B to be in a sealed state. In this sense, the means for sticking may be any one that maintains a sealed state regardless of adhesion, adhesion, fusion, or the like.
For this film B, for example, a permeable adhesive tape TBAT-252 of TRUSCO can be adopted, which has excellent air permeability of 170 cc / cm ² · sec and can prevent invasion of various bacteria.

And, as described above, the film A and the film B constituting the combination film body C can be made relatively inexpensive because, for example, the film A can be composed of a polyvinyl chloride film or a polyethylene film, It will be economical.

By the way, when hyphae spread in the above-mentioned culturing step, primordium formation is started.
In the formation of the primordium, light irradiation is required in addition to the above-mentioned oxygen supply.
The reason for this is that in primordium formation, mushroom growth switches from vegetative growth to reproductive growth, and the presence of this light stimulates and triggers the switching of growth stages. .
However, conventionally, since a translucent material is generally used, the illuminance after passing through the material is reduced to about 83%, which causes a delay in primordium formation and hinders shortening of the culture period.
On the other hand, the film A as a component of the combined film body C of the present invention has translucency as well as extensibility, so that it exhibits excellent translucency and can meet the above-mentioned requirement of light irradiation. Become.

In response to the light irradiation requirement, natural light may be used as the light source, but artificial lighting is performed as necessary.
In the case of using natural light, a space where natural light can be emitted is secured at the upper part of the container body 2, and in the case of artificial lighting, a lighting device 5 such as an LED is provided at the upper part.

Now, as the primordium formation proceeds, a phenomenon in which the culture medium 3 rises 3a may be seen on the surface of the container, as shown in FIG. 5 (e).
The reason is presumed as follows.
When the mycelium spreads throughout the medium, the mycelium forms a coating for primordium formation, increases the amount of mycelium inside the medium, and forms a mycelial mass from the top of the bacterial bed to enhance nutrient accumulation. There is. In other words, it is considered that the space required for securing the amount of mycelium forming the primordium cannot be secured only in the medium, and the formed mycelial mass expands the medium and raises the surface of the medium 3.
Therefore, suppressing the formation of the mycelial mass inhibits an increase in the amount of mycelium, which results in the prevention of proper primordium formation.
On the other hand, as described above, the film body C of the present invention is combined with the film A exhibiting an excellent elongation rate, so that good extensibility can be exhibited.
Therefore, when the culture medium 3 rises 3a due to the formation of a mycelial mass, the combination film body C having the extensible film A follows the rise, and an appropriate base material is not hindered from increasing the mycelium mass. It promotes group formation.

When the formation of the primordium is completed, for example, the entire surface of the culture medium starts to turn brown, the process proceeds to the growth step of promoting the growth of fruiting bodies with the combination film C removed from the container body 2 (FIG. 5 (f)). reference).
As the primordium formation nears the end, a change was observed in which almost the entire surface of the medium turned brown, which is also evidence that primordium formation was almost completed. The primordium formation is almost completed, and it is the time when young fruit bodies can be seen in the early stage of fruit body development.
Therefore, the time when the formation of the primordium is completed or the time when the occurrence of the juvenile body is observed is regarded as the completion of the formation of the primordia, and at this time, the fixing means 4 is released and the combined film body C is moved to the container body 2. , And moved to a development room at about 15 ° C. to promote the growth of fruiting bodies.
Here, removing the combined film body C from the container body 2 means removing the state where the film body seals the container, and also includes cutting the film body to expose the medium.
When the film body and the mushroom adhere to each other when the combined film body C is removed, mushroom generation is damaged. However, if the surface of the combined film body C of the present invention is made flat, there is no adhesion with the mushroom. Become.
This step is basically an environment that is the same as the growth of normal fruiting bodies, but the growth of fruiting bodies after sufficient primordium formation is not hindered by the increase in the amount of mycelium and proper growth Growth of many fruiting bodies has been confirmed.

  When the fruiting bodies mature, they are collected and used as mushroom products (see FIG. 5 (g)).

As described above, according to the present invention, depending on the cultivation container used, for example, as shown in FIG. 6 (A), when the self-supporting leg 2a is arranged at an appropriate length at the bottom of the container, the container body can be piled up. In addition, as shown in FIG. 6 (B), the height of the shelf can be reduced even when the shelf is stacked. Therefore, a larger number of cultivation containers can be installed in the cultivation room, and the cultivation containers can be efficiently stored in the room and the storage density can be increased.
At the same time, as described above, when the culture medium rises, the combination film body C having the film A with high malleability can be elongated following the growth of the culture medium, and the mycelium is suppressed by suppressing the mycelial mass. The developmental drawback of inhibiting the increase in volume can be overcome.
Therefore, it is possible to satisfy both requirements of a high storage density and ensuring good conditions for generating mushrooms.

  In addition, from the securing of sufficient oxygen supply and the uniformity of the entire culture medium, the mushroom generation site can be homogenized, and sufficient light is administered by translucency, so that proper primordium formation Is promoted and the cultivation period can be shortened as described above.

<Example>
The target was shiitake mushroom, and as a medium suitable for this, 10 wt% of the weight of the medium as a nutrient was added to and mixed with hardwood sawfish and water was added to adjust the water content to 62 wt%.
A 150 × 100 × 35 mm (146 × 102 × 32 mm inside) box-shaped polypropylene container body was filled with the above-mentioned medium in the form of a flat top-cut surface, which was covered with a combination film C, Tied up. This was subjected to steam sterilization at 120 ° C. for 60 minutes.
The combined film body C was composed of a film A made of Hitachi Wrap and a film B made of TRUSCO's breathable adhesive tape TBAT-252.
After cooling, the film was once removed, inoculated with Shiitake mushrooms, and ligated again.
This was cultured in a room maintained at 20 ° C. and RH 60 to 80% for 60 days. Lighting was performed during the operation by turning on an indoor lighting lamp, and irradiation was performed at 200 to 300 lux near the surface of the culture medium for 0.5 to 4 hours a day.

On the 30th day, the growth of the culture medium started to reach a height of about 10 mm, but the combined film C covered with it expanded, followed by the browning of the medium surface on the 45th day, and furthermore, Group formation proceeded.
The bacterial bed after the completion of the cultivation was transferred to a development room controlled at 15 ° C. and RH 80 to 90%, and the film was removed and immersed for 24 hours. On the 10th day thereafter, mushrooms (shiitake) were obtained (FIG. 7).

At this time, the mushroom fresh weight of only the first generation was 20% to 25% per unit medium weight, and the cultivation period including the cultivation period and the development period was 70 days.
In the case of a commonly used 3 Kg bacterial bed, the mushroom fresh weight that can be harvested during a cultivation period of 220 days, which is a combination of a culture period of 100 days and a development period of 120 days, is 700 to 1000 g, and 23% to 30%.
When comparing the wrap cultivation this time with the existing 3 kg bag cultivation, the mushrooms with the same fresh weight when compared per unit medium weight, and the cultivation period less than 30% in the wrap cultivation compared with the existing bag cultivation This is what was obtained.

  At the time of completion of the culture, there was no harmful bacterial contamination, and the bacterial barrier property as a filter of the film was confirmed.

1. Cultivation container 2 Container main body 2a Leg 3 Medium 3a Rising 4 Fixing means 5 Lighting device A Extensible film a Vent B Oxygen permeable film C Combined film

Claims (4)

A container body that has shape retention and can be filled with a certain amount of medium by arranging an opening on the top surface, and a vent hole a in a film A that is extensible and translucent without requiring oxygen permeability. A combination film C formed by attaching a film B having pores that allow oxygen to pass through but do not allow bacteria to pass through the ventilation holes a, and fixing means for fixing the film to the container body. Using a cultivation container,
a) Filling the container body with the sterilized medium, or sterilizing and filling the medium to sterilize the container body after filling the medium;
b) a seed inoculation step of spraying the seed on the surface of the culture medium, covering the upper surface of the container body with the above-mentioned combination film C, and tightly fixing the mixture with fixing means;
c) an initial stage of hyphal culture in which the inoculated mycelium is spread on the medium in an oxygen-permeable and translucent environment;
d) When primordium formation is initiated by the spread of mycelia and the culture medium rises due to the formation of a hyphal mass, the combination film body C having spreadability follows the rise and prevents an increase in the amount of mycelium. A late stage of mycelium culture that promotes primordium formation without
e) When the primordium formation is completed, a fruiting body growing step of promoting the growth of fruiting bodies with the film body removed from the container body;
f) a harvesting process for harvesting mature fruiting bodies;
A method for cultivating a mushroom using an extensible film, comprising:   2. The step of filling a culture medium according to claim 1, wherein the culture medium is flattened into a top surface of a container or filled at least to a height of 20 mm or less from a top end of the container. Cultivation method.   3. A method for cultivating a mushroom using an extensible film, wherein the combination film body according to claim 1 has a smooth surface having no adhesion to the mushroom. A method for cultivating a mushroom using an extensible film, wherein a lighting device is provided on an upper portion of the container body according to claim 1.