JPH09187168A - Bag for fungus incubation and the incubation of the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a bag for fungus incubation and incubating method able to gather fruiting body in high yield at early state by feeding a proper air for the growth of hypha from the stage of incubation, formation of primordium and fruiting body and keeping the high growth vitality (breathing vitality) of mycelium. SOLUTION: This bag for incubation 5 is made of plastic film having a gas permeable porous sheet 3 thermo compression bonded on the venting hole and the bond strength of the thermo compression bonding is 0.1-1.5kgf/15mm according to tensile tester. The bag is filled with culturing raw material 6 and planted with a bacterial strain and the gas permeable sheet 3 is peeled off from the bag when the hypha spreads to the 50% length from culturing medium to top edge of the bag and continued the incubation.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a mushroom bag for industrially mass-producing mushrooms and a method for cultivating mushrooms using the same.

[0002]

2. Description of the Related Art In recent years, as a method for industrially producing edible mushrooms, a plastic bag is sterilized by filling mushroom medium prepared by hydrolyzing sawdust, rice bran, etc., and inoculated with inoculum. After that, a part or all of the bag is torn to expose the fungus bed and to generate mushrooms.

In the above-mentioned process, in the culturing process, mushroom hyphae elongated from the inoculum are spread on the medium, and after the spread, additional culture is carried out in the bag to induce the primordium of the fruiting body, and then the culture bag is partially Alternatively, the whole bag is crushed and subjected to a cultivation process to form fruit bodies. Therefore, the mushroom mycelia will be cultured not only in the process of infesting the medium but also in the environment in the culture bag for a long time after the infestation is completed. The in-bag culture period (including additional culture) varies depending on the type of mushroom and the size of the medium, but ranges from 20 to 120 days.

Therefore, as a bag for cultivating the mushrooms, a filter is attached to the side surface of a plastic bag, and air is circulated in the bag for a long period of time while preventing invasion of various bacteria from the filter. It is being appreciated. For example, the mushroom culture bag of Japanese Patent Publication No. 57-42287 is a olefin plastic bag provided with a porous plastic film having a specific hole diameter and porosity in a part thereof. In addition, the mushroom fungus culture growth container disclosed in Japanese Utility Model Publication No. 4-23393 has a container body provided with a vent hole and a water-repellent sterilized paper having a specific air permeability attached thereto.

[0005] However, since the amount of bacterial cells increases with the spread of mycelium and the respiratory volume increases, a single filter cannot supply a sufficient amount of air (oxygen). Therefore, it takes a long time for culturing, the formation of primordia is delayed, and the yield of fruiting bodies is decreased.

That is, in the conventional culture bag with a filter, air is sufficient in the medium at the time of inoculation of the inoculum and the amount of mycelia is small. Therefore, the air flow rate is larger than the respiration rate of the mycelia and the growth of mycelia is good. However, as the hyphae grow, the amount of air supplied from the filter becomes insufficient, and the amount of oxygen in the bag decreases, reaching a peak. After that, it is considered that the vitality of the mycelium is reduced in the so-called oxygen deficient state and the total respiration volume is also reduced. Therefore, in the conventional culture method using a filter, the air flow rate of the filter can be supplied to a certain required amount from the early stage of culture to the middle stage, but becomes insufficient from the late stage of culture to fruit body formation.

[0007] In order to improve this point, taking out the culture medium from the culture bag in the initial stage of the culture has a drawback that various bacteria are contaminated and the culture medium is significantly dried.

[0008]

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and its purpose is from culture to primordium formation or fruit body formation.
Mushroom culture bag and bag that can supply the proper air for mycelial growth, maintain high mycelial growth vitality (respiratory activity), and collect high-yield fruiting bodies at an early stage A method for cultivating mushrooms is provided.

[0009]

[Means for Solving the Problems] The above-mentioned object is a culture bag in which a breathable porous sheet is thermocompression-bonded to an opening for ventilation of a culture bag made of a plastic film. Saga tensile tester 0.1-1.5kgf /
A mushroom bag for cultivating mushrooms, characterized by having a length of 15 mm, and a method for cultivating mushrooms, which comprises culturing mushroom bags after inoculating mushroom seeds with a medium raw material filled in the culture bag. A method for cultivating mushrooms characterized in that (A) is used, and the air-permeable porous sheet of the culture bag (A) is peeled off and further cultured when the mushroom hyphae have spread 50% or more from the top surface of the medium. To be achieved. (A) A culture bag in which a breathable porous sheet is thermocompression bonded to an opening for ventilation of a culture bag made of a plastic film, the thermocompression strength being 0.1 to 10 in a tensile tester.
A culture bag for mushrooms that is 1.5 kgf / 15 mm.

That is, the inventors of the present invention set the bonding (thermocompression) strength between the mushroom culture bag and the air-permeable porous sheet within a specific range to facilitate peeling (hereinafter referred to as "easy peelability"). Then, the culture bag and the air-permeable porous sheet can be easily and arbitrarily opened. Then, the air-permeable porous sheet was peeled off without damaging the culture substrate or the culture bag to adjust the amount of air to promote mycelial growth and shorten the culture period. Furthermore, by removing the breathable porous sheet after a specific culture state, it was possible to supply a sufficient amount of air while preventing contamination of various bacteria.

[0011]

Next, the present invention will be described in detail.
FIG. 1 is an explanatory view showing an example of the mushroom culture bag of the present invention. In FIG. 1, reference numeral 1 denotes a bag made of a plastic film formed by folding a gusset into a bag shape. An opening 2 for ventilation is formed in the bag 1, and the air-permeable porous sheet 3 is thermocompression-bonded to the opening 2 for ventilation to form a joint 4.

Bonding strength of this bonded portion 4 (strength of thermocompression bonding)
Is required to be set to 0.1 to 1.5 kgf / 15 mm, more preferably 0.2 to 1.0 kgf / 15 mm by a method using a tensile tester specified in JIS-Z0238.

When the bonding strength is less than 0.1 kgf / 15 mm, the air-permeable porous sheet is easily peeled off when the culture bag is filled with the medium or the inoculation work is performed, which is not preferable. On the other hand, if the bonding strength exceeds 1.5 kgf / 15 mm, the breathable porous sheet is difficult to peel off, and depending on the material, the breathable porous sheet or the culture bag body is likely to tear, which is not preferable.

The material of the air-permeable porous sheet is not particularly limited as long as the thermocompression bonding to the culture bag can be set within the above-mentioned bonding strength range. It is possible to obtain a desired peel strength by using a mixture of synthetic pulp typified by polyethylene and the like and wood pulp, and adjusting the mixing ratio of the heat-melting synthetic pulp and the wood pulp having no heat-sealing property. . In addition, olefin plastics such as polyethylene and polypropylene, polyester, nylon,
It may be formed of vinyl, polyvinyl chloride or the like, and of these, a sheet obtained by laminating sheets having different physical properties such as melting temperature and density is used as a breathable porous sheet, and each sheet is peeled off when peeled. A predetermined bonding strength may be obtained by separating the layers of the material.
When the main raw material is a natural product such as wood pulp that does not have the meltability by thermocompression bonding, if a hot-melt type paste is used, easy peeling can be imparted by interfacial peeling.

Further, the air-permeable porous sheet has a hole diameter of 0.1 to 100 μm and an air permeability of 2 according to JIS-P8117 so as to have the air flowability necessary for the growth of mycelia of mushrooms. It is preferable to set it in the range of 200 sec / 100 cc from the viewpoint of air flowability and control of various bacteria, and more preferably, hole diameter 1-100 μm, air permeability 60-150.
Seconds / 100 cc. The area and thickness of the air-permeable porous sheet depends on the performance of the air-permeable porous sheet, for example, when the air flowability is poor, the area of the sheet is wide,
If the thickness is thin, conversely the air circulation is too good, the environment in the culture room is bad (there are many floating microorganisms), and the contamination ratio of various bacteria is a concern, reduce the area of the sheet and increase the thickness. It may be set appropriately.

The shape of the ventilation openings provided in the air-permeable porous sheet and the culture bag is not particularly limited, and may be appropriately set such as a circle, an ellipse, a polygon such as a triangle or a quadrangle, and a linear shape. Good.

The size of the ventilation opening is not particularly limited, and may be appropriately set in accordance with the volume ratio of the culture medium and the culture bag so that the required ventilation amount can be obtained. For example, almost the entire surface of the culture bag may be set as the ventilation opening. However, in this case, it is preferable that the air-permeable porous sheet is peeled off only in the culture bag space portion, that is, the portion that is not in contact with the culture raw material, from the viewpoint of preventing contamination of various bacteria.

The size and shape of the breathable porous sheet are
From the viewpoint of facilitating peeling from the culture bag, it is desirable to form a non-bonded margin on the side of the air-permeable porous sheet by making it larger than the opening for ventilation or changing the shape. For example, the ventilation openings are circular, and the length of one side of the ventilation porous sheet is set to a quadrangle slightly larger than the diameter of the ventilation openings to facilitate peeling from the four corners of the ventilation porous sheet. You may do it.

Alternatively, as shown in FIG. 3, a standing pouch-type plastic film bag 11 may be used as a culture bag in which a linear breathable porous sheet 12 is thermocompression-bonded and fixed. In this case, as shown in FIG.
By peeling the air-permeable porous sheet 12, the culture bag 1
Since the upper part of 3 can be divided, it is suitable for cultivating mushrooms such as oyster mushrooms, beech slugs and nameko that produce fruiting bodies from the top surface of the culture medium 14.

Cultivation of mushrooms using the above culture bag is
For example, it is performed as follows. First, a mushroom bag obtained by mixing water with sawdust, rice bran, or the like and adjusted with water is filled in the culture bag obtained as described above. Then, heat sterilization is performed, and after the sterilization is completed, the medium is cooled, and then a desired mushroom inoculum such as shiitake, nameko, maitake, etc. is inoculated. Then, the upper opening of the culture bag is thermocompression-bonded and sealed, and the culture is performed as shown in FIG.

When inoculated with mushroom inoculum and cultivated, the mycelium of mushroom grows respiration by the circulation of air through the air-permeable porous sheet and extends to about 30% of the whole medium. When the culture is further continued, as shown in FIG. 5 (A), the mushroom hyphae-growing portion 22 moves from the culture medium top surface 21 toward the culture medium bottom surface 24,
It grows up to 50% to 65% of the total height 19 of the medium,
The oxygen consumption due to the respiration of mushroom hyphae in the culture bag 18 exceeds the peak, and the air supply through the air-permeable porous sheet 15 cannot be made in time, so-called oxygen deficiency state begins and the hyphae vigor is reduced. Therefore, it is desirable to peel off a part or all of the air-permeable porous sheet 15 after this time to promote the circulation of air.

In the state of FIG. 2 (B), even if the amount of fungal mycelium of the mushroom mycelium on the top surface of the medium and its secreted product are removed by removing the air-permeable porous sheet to some extent,
Miscellaneous bacteria cannot reproduce and are in a state of being less susceptible to contamination.

The timing of peeling the air-permeable porous sheet is at the peak of the oxygen consumption in the culture bag described above in order to maintain the vitality of the mushroom hyphae at a high level, obtain early fruiting bodies, and obtain a high yield. Or, it is desirable that it is immediately after the peak, but it may be appropriately changed depending on the mycelial growth rate of the mushrooms to be cultured, the resistance to various bacteria, or the environment of the culture room. For example, it may be performed after the completion of the mycelial spread.

Regarding the area for peeling off the air-permeable porous sheet, it is desirable to peel off the entire area from the viewpoint of air circulation, but depending on the hyphae growth rate and resistance to various bacteria of the mushrooms to be cultured, or the environment of the culture room. Therefore, if the environment in the culture room is easily dried, for example, in FIG.
You may make it peel off only one part like (B).

By culturing as described above,
The air circulation to the culture bag is promoted, and the vitality of mushroom mycelia is dramatically improved. Moreover, since the breathable porous sheet having a specific bonding strength is used, it can be easily and surely peeled off even by manual work. Therefore, unlike the case where the same work is carried out using a cutter or scissors, the breakage is not uneven, the culture medium is not damaged, and the culture medium can be prevented from being dried.

Next, when culturing is continued in this state, as shown in FIG.
As shown in (B), after the hyphae spread throughout the medium,
As shown in FIG. 2C, the fruiting body primordium 9 is derived.
Then, the upper part of the culture bag is broken during the growth of the fruiting body, and the desired mushroom fruiting body 10 is obtained as shown in FIG. 2 (D). When the fruiting bodies are generated from the entire surface of the mushroom culture medium, the entire culture bag may be broken.

[0027]

INDUSTRIAL APPLICABILITY As described above, the mushroom bag for cultivating mushrooms of the present invention is provided with a vent hole in a part of the plastic film bag,
Since a breathable porous sheet is thermocompression-bonded to that part so that it has a specific bonding strength, by removing the breathable porous sheet of the mushroom bag for culture after a specific culture condition, contamination of various bacteria is possible. It is possible to supply a sufficient amount of air while preventing the above, and it is possible to dramatically shorten the culture period as compared with the conventional culture bag, and it is possible to mass-produce the mushroom culture medium with high yield.

Next, the present invention will be specifically described based on examples. <Preparation of culture bag> Cylindrical extruded diameter 12
A transparent polypropylene film having a thickness of 50 cm and a thickness of 50 cm was cut into a length of 35 cm. Then, this cylindrical film was gusset-folded, and the bottom portion was heat-sealed and hermetically sealed to form a bag. Next, a vent hole having a diameter of 2 cm was opened around the position 80 cm from the bag opening. On the other hand, a breathable porous sheet (60% by weight high-density polyethylene and 40% by weight wood pulp mixed 0.12 mm thick, 3 mm average hole diameter, 100 seconds air permeability (JIS-P8117) sterilized paper) is prepared. ,
Thermocompression bonding was performed from the outside of the culture bag to the ventilation holes of the culture bag. The bonding strength at this time is 0.4 kgf according to JIS-Z0238.
/ 15 mm.

<Preparation of Shiitake Culture Medium> Into the mushroom culture bag described above, 1 part of rice bran having 4 parts by weight of sawdust (hereinafter referred to as “part”) was added and 1 kg of a medium having a water content adjusted to 70% was filled.
Next, this is put in a heat sterilization kettle, and 90 at 120 ° C
Hold for minutes to sterilize. Then, take this out into a clean room, cool it until the medium temperature reaches 25 ° C, inoculate 5 g of Shiitake inoculum, close the upper opening of the culture bag in one letter as shown in Fig. 2 (A), and seal with heat seal. Then, the cells were cultured. The environment of the culture room was controlled at 20 to 25 ° C, and the floor surface was kept clean.

<Examples 1 to 5> Using the Shiitake mushroom culture medium in a culture bag prepared as described above, 10 days after inoculation, 2
On days 0, 30, 40, and 60, the air-permeable porous sheet of the culture bag was peeled off (Examples 1, 2, 3, 4,
5) Further, the culture was continued until the 80th day after the inoculation, the culture bag was broken, and then the culture bag was cultivated in the cultivation house.
Then, after the fruit body collection is completed, it is flooded on the 110th day,
Prompted the second fruiting body development. The temperature conditions in the cultivation house are natural (maximum 25 ° C, minimum 10 ° C, average 15 ° C)
I went in. Each test was performed using 300 culture media, and fruit body collection was terminated on the 140th day.

Examples 6 to 8 Example 2 was repeated except that the bonding strength of the air-permeable porous sheet with the culture bag was set to the value shown in Table 1.
Same as.

<Example 9> The same procedure as in Examples 1 to 5 was repeated, except that the air-permeable porous sheet of the culture bag was not removed and the culture was continued until 80 days after the inoculation.

<Comparative Example 1> In the preparation of the culture bag, the strength of the culture bag ventilation hole and the air-permeable porous sheet at the time of thermocompression bonding was set to 0.05 kgf / 15 mm in a tensile tester, and the same manner as in Example 2. I did the same.

Comparative Example 2 In the preparation of the culture bag, the air-permeable porous sheet was made of 100% polypropylene, and the strength of the culture bag ventilation hole and the air-permeable porous sheet during thermocompression bonding was measured by a tensile tester. The same procedure was performed as in Example 2 except that the pressure was 0 kgf / 15 mm. The above results are shown in Tables 1 and 2.

[0035]

[Table 1]

[0036]

[Table 2]

As is clear from Table 1 and Table 2, Example 1
Since the air-permeable porous sheet was peeled from the mushroom culture bag when the hyphae spread 20% from the top surface of the culture medium, contamination of miscellaneous bacteria was observed in the portion where the hyphae did not grow, and Although not shown in the figure, some variation in the fruit body yield among the culture media was observed. However, the fruiting body yield was high, and the maximum object of the present invention to obtain a high yield in a short period of time was sufficiently achieved.

Each of the culture media obtained in Examples 2 to 4 had a high fruiting body yield, and there was almost no contamination by various bacteria as compared with Example 1. From this, in the process of culturing, peeling the air-permeable porous sheet when the hyphae have spread 50% or more from the top surface of the medium means that the air flow into the culture bag is sufficient and the hyphae are activated. It can be seen that it is preferable because it promotes the resistance to contamination by various bacteria, the fruiting body is generated early, and the yield is improved.

In Example 5, the air-permeable porous sheet was peeled from the mushroom culture bag after culturing for up to 60 days after inoculation even after the mycelium was infested. It can be seen that the fruiting body yield is not as high as in Examples 1 to 4, because the vigor of the hyphae is once reduced and the viability of the hyphae is once reduced.

In Examples 6 to 8, the bonding strength of the air-permeable porous sheet was changed in Example 2, but if the bonding strength is within the range of 0.1 to 1.5 kgf / 15 mm, It can be seen that the same results as in Example 2 are obtained.
However, in Example 8, the bonding strength was 1.5 kgf / 15 m.
Since it has a strong m, it has an easy peeling property, but when peeling the breathable porous sheet from the culture bag, it was necessary to open it carefully so as not to damage the culture bag.

In Example 9, since the permeable porous sheet was cultured without being peeled off and bonded, the fruiting body yield was not as high as that of the other Examples.

On the other hand, in Comparative Example 1, since the bonding strength between the air-permeable porous sheet and the culture bag is low, the air-permeable porous sheet is used for filling the culture bag with the medium, the steam sterilization and cooling process, and the inoculation work. A part or all of the sheet was peeled off, and a large amount of contaminants was generated in the early stage of culture.

Further, in Comparative Example 2, since the bonding strength was strong, it took not only time to peel off the air-permeable porous sheet, but also the air-permeable porous sheet and the culture bag were torn, resulting in contamination of various bacteria. Furthermore, regarding the culture medium that was grown up without contamination with various bacteria, the top surface of the culture medium was dried during the culture process, and the yield of fruiting bodies was drastically reduced.

[Brief description of the drawings]

FIG. 1 is an explanatory view showing an example of a culture bag for mushrooms of the present invention.

FIG. 2 is an explanatory view showing a process of culturing to cultivating using the mushroom bag of the present invention.

FIG. 3 is an explanatory view showing another example of the mushroom culture bag of the present invention.

FIG. 4 is an explanatory view showing a state in which the air-permeable porous sheet of the mushroom bag of FIG. 3 is peeled off.

FIG. 5 is an explanatory view showing a state in which mycelium grows by culturing using the mushroom bag of the present invention.

[Explanation of symbols]

1 Plastic bag made by folding a gusset into a bag 2 Opening part for ventilation 3 Breathable porous sheet 4 Joining part 5 Culture bag 6 Medium 7 Mushroom mycelium 8 Heat seal part 9 Fruit body primordium 10 Mushroom Fruiting body 11 Standing pouch type plastic film bag 12 Linear breathable porous sheet 13 Culture bag 14 Culture medium 15 Breathable porous sheet 16 Venting opening 17 Sealing portion 18 Culture bag 19 Total medium height 20 Inoculum 21 medium top surface 22 mycelial growth part 23 medium 24 medium bottom 25 mushroom culture medium in which mycelium has spread

Claims (2)

[Claims] 1. A culture bag in which an air-permeable porous sheet is thermocompression bonded to an aeration opening of a culture bag made of a plastic film, the thermocompression strength being 0.1 in a tensile tester. A culture bag for mushrooms, which is ~ 1.5 kgf / 15 mm. 2. A method of cultivating mushrooms, which comprises culturing a mushroom bag in which a culture medium material is filled, inoculated with a mushroom inoculum, and then cultivated, wherein the following culture bag (A) is used as the culture bag. ) A method for cultivating mushrooms, characterized in that the breathable porous sheet (1) is peeled off and further cultured when the mushroom hyphae have spread from the top surface of the medium by 50% or more. (A) A culture bag in which a breathable porous sheet is thermocompression bonded to an opening for ventilation of a culture bag made of a plastic film, the thermocompression strength being 0.1 to 10 in a tensile tester.
A culture bag for mushrooms that is 1.5 kgf / 15 mm.