JP2019521139A5 - - Google Patents
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- JP2019521139A5 JP2019521139A5 JP2019501575A JP2019501575A JP2019521139A5 JP 2019521139 A5 JP2019521139 A5 JP 2019521139A5 JP 2019501575 A JP2019501575 A JP 2019501575A JP 2019501575 A JP2019501575 A JP 2019501575A JP 2019521139 A5 JP2019521139 A5 JP 2019521139A5
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- alkyl
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- 125000000217 alkyl group Chemical group 0.000 claims description 216
- 150000001875 compounds Chemical class 0.000 claims description 109
- 238000010362 genome editing Methods 0.000 claims description 72
- 108090000623 proteins and genes Proteins 0.000 claims description 70
- 210000004027 cell Anatomy 0.000 claims description 64
- 239000013078 crystal Substances 0.000 claims description 58
- 125000001153 fluoro group Chemical group F* 0.000 claims description 54
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 54
- 229910052731 fluorine Inorganic materials 0.000 claims description 49
- WNLRTRBMVRJNCN-UHFFFAOYSA-N adipic acid Chemical compound OC(=O)CCCCC(O)=O WNLRTRBMVRJNCN-UHFFFAOYSA-N 0.000 claims description 48
- 102000004169 proteins and genes Human genes 0.000 claims description 42
- 150000007523 nucleic acids Chemical class 0.000 claims description 41
- 150000003839 salts Chemical class 0.000 claims description 41
- 229910052739 hydrogen Inorganic materials 0.000 claims description 40
- 239000001257 hydrogen Substances 0.000 claims description 40
- 239000002773 nucleotide Substances 0.000 claims description 38
- 125000003729 nucleotide group Chemical group 0.000 claims description 38
- 125000000623 heterocyclic group Chemical group 0.000 claims description 36
- 150000002431 hydrogen Chemical class 0.000 claims description 36
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 36
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 32
- 108020004707 nucleic acids Proteins 0.000 claims description 32
- 102000039446 nucleic acids Human genes 0.000 claims description 32
- 108091033409 CRISPR Proteins 0.000 claims description 28
- 239000013598 vector Substances 0.000 claims description 26
- 239000001361 adipic acid Substances 0.000 claims description 25
- 235000011037 adipic acid Nutrition 0.000 claims description 25
- 230000014509 gene expression Effects 0.000 claims description 24
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 claims description 22
- 229910052805 deuterium Inorganic materials 0.000 claims description 22
- 230000007018 DNA scission Effects 0.000 claims description 21
- 238000010354 CRISPR gene editing Methods 0.000 claims description 20
- 230000037361 pathway Effects 0.000 claims description 20
- 125000006555 (C3-C5) cycloalkyl group Chemical group 0.000 claims description 18
- 125000000753 cycloalkyl group Chemical group 0.000 claims description 18
- 125000002757 morpholinyl group Chemical group 0.000 claims description 18
- 125000003566 oxetanyl group Chemical group 0.000 claims description 18
- 125000003718 tetrahydrofuranyl group Chemical group 0.000 claims description 18
- 125000001412 tetrahydropyranyl group Chemical group 0.000 claims description 18
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 15
- 230000008439 repair process Effects 0.000 claims description 15
- 230000006780 non-homologous end joining Effects 0.000 claims description 14
- 108020004414 DNA Proteins 0.000 claims description 13
- 108020005004 Guide RNA Proteins 0.000 claims description 12
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 10
- 239000012190 activator Substances 0.000 claims description 10
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 10
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 10
- 239000013603 viral vector Substances 0.000 claims description 10
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims description 8
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims description 8
- 230000000295 complement effect Effects 0.000 claims description 6
- 230000002401 inhibitory effect Effects 0.000 claims description 6
- 239000005711 Benzoic acid Substances 0.000 claims description 5
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 5
- 235000010233 benzoic acid Nutrition 0.000 claims description 5
- 239000001530 fumaric acid Substances 0.000 claims description 5
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims description 5
- 239000011976 maleic acid Substances 0.000 claims description 5
- 101710172824 CRISPR-associated endonuclease Cas9 Proteins 0.000 claims description 4
- 102220605874 Cytosolic arginine sensor for mTORC1 subunit 2_D10A_mutation Human genes 0.000 claims description 4
- 102000053602 DNA Human genes 0.000 claims description 4
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 4
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 4
- 101710163270 Nuclease Proteins 0.000 claims description 4
- 101000910035 Streptococcus pyogenes serotype M1 CRISPR-associated endonuclease Cas9/Csn1 Proteins 0.000 claims description 4
- 230000022131 cell cycle Effects 0.000 claims description 4
- 229940125904 compound 1 Drugs 0.000 claims description 4
- 229940125782 compound 2 Drugs 0.000 claims description 4
- 230000005782 double-strand break Effects 0.000 claims description 4
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 claims description 4
- 239000012636 effector Substances 0.000 claims description 4
- 230000001404 mediated effect Effects 0.000 claims description 4
- 238000002703 mutagenesis Methods 0.000 claims description 4
- 231100000350 mutagenesis Toxicity 0.000 claims description 4
- 239000013612 plasmid Substances 0.000 claims description 4
- 241000702421 Dependoparvovirus Species 0.000 claims description 3
- 241000713666 Lentivirus Species 0.000 claims description 3
- 241000700584 Simplexvirus Species 0.000 claims description 3
- 239000001384 succinic acid Substances 0.000 claims description 3
- 230000002103 transcriptional effect Effects 0.000 claims description 3
- 241000701161 unidentified adenovirus Species 0.000 claims description 3
- 241001430294 unidentified retrovirus Species 0.000 claims description 3
- 108020004635 Complementary DNA Proteins 0.000 claims description 2
- 241000124008 Mammalia Species 0.000 claims description 2
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 claims description 2
- 229940126214 compound 3 Drugs 0.000 claims description 2
- 210000004748 cultured cell Anatomy 0.000 claims description 2
- 230000003247 decreasing effect Effects 0.000 claims description 2
- 230000037430 deletion Effects 0.000 claims description 2
- 238000012217 deletion Methods 0.000 claims description 2
- 238000001727 in vivo Methods 0.000 claims description 2
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 230000010354 integration Effects 0.000 claims description 2
- 230000035772 mutation Effects 0.000 claims description 2
- 108091033319 polynucleotide Proteins 0.000 claims description 2
- 102000040430 polynucleotide Human genes 0.000 claims description 2
- 239000002157 polynucleotide Substances 0.000 claims description 2
- 230000001360 synchronised effect Effects 0.000 claims description 2
- -1 1 Chemical class 0.000 claims 10
- 238000005245 sintering Methods 0.000 claims 4
- GHYOCDFICYLMRF-UTIIJYGPSA-N (2S,3R)-N-[(2S)-3-(cyclopenten-1-yl)-1-[(2R)-2-methyloxiran-2-yl]-1-oxopropan-2-yl]-3-hydroxy-3-(4-methoxyphenyl)-2-[[(2S)-2-[(2-morpholin-4-ylacetyl)amino]propanoyl]amino]propanamide Chemical compound C1(=CCCC1)C[C@@H](C(=O)[C@@]1(OC1)C)NC([C@H]([C@@H](C1=CC=C(C=C1)OC)O)NC([C@H](C)NC(CN1CCOCC1)=O)=O)=O GHYOCDFICYLMRF-UTIIJYGPSA-N 0.000 claims 1
- 208000009889 Herpes Simplex Diseases 0.000 claims 1
- 229940125797 compound 12 Drugs 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 230000001177 retroviral effect Effects 0.000 claims 1
- 230000001629 suppression Effects 0.000 claims 1
- 108091006106 transcriptional activators Proteins 0.000 claims 1
- 230000035899 viability Effects 0.000 claims 1
- 238000000034 method Methods 0.000 description 54
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662361961P | 2016-07-13 | 2016-07-13 | |
| US201662361781P | 2016-07-13 | 2016-07-13 | |
| US62/361,781 | 2016-07-13 | ||
| US62/361,961 | 2016-07-13 | ||
| PCT/US2017/041979 WO2018013840A1 (en) | 2016-07-13 | 2017-07-13 | Methods, compositions and kits for increasing genome editing efficiency |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019521139A JP2019521139A (ja) | 2019-07-25 |
| JP2019521139A5 true JP2019521139A5 (enExample) | 2020-08-20 |
| JP7033583B2 JP7033583B2 (ja) | 2022-03-10 |
Family
ID=59388213
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019501575A Active JP7033583B2 (ja) | 2016-07-13 | 2017-07-13 | ゲノム編集効率を高めるための方法、組成物及びキット |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US11124805B2 (enExample) |
| EP (2) | EP3484870B1 (enExample) |
| JP (1) | JP7033583B2 (enExample) |
| CN (1) | CN109863143B (enExample) |
| AU (2) | AU2017295720B2 (enExample) |
| CA (1) | CA3030783A1 (enExample) |
| ES (1) | ES2938210T3 (enExample) |
| MA (1) | MA45670A (enExample) |
| WO (1) | WO2018013840A1 (enExample) |
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2017
- 2017-07-13 AU AU2017295720A patent/AU2017295720B2/en active Active
- 2017-07-13 CN CN201780052987.3A patent/CN109863143B/zh active Active
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- 2017-07-13 EP EP17743188.9A patent/EP3484870B1/en active Active
- 2017-07-13 WO PCT/US2017/041979 patent/WO2018013840A1/en not_active Ceased
- 2017-07-13 EP EP22207527.7A patent/EP4219462A1/en not_active Withdrawn
- 2017-07-13 MA MA045670A patent/MA45670A/fr unknown
- 2017-07-13 JP JP2019501575A patent/JP7033583B2/ja active Active
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