JP2019517778A5 - - Google Patents
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- Publication number
- JP2019517778A5 JP2019517778A5 JP2018552671A JP2018552671A JP2019517778A5 JP 2019517778 A5 JP2019517778 A5 JP 2019517778A5 JP 2018552671 A JP2018552671 A JP 2018552671A JP 2018552671 A JP2018552671 A JP 2018552671A JP 2019517778 A5 JP2019517778 A5 JP 2019517778A5
- Authority
- JP
- Japan
- Prior art keywords
- volume
- aqueous composition
- lysis buffer
- buffer
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012139 lysis buffer Substances 0.000 claims 13
- 239000000203 mixture Substances 0.000 claims 13
- 238000000034 method Methods 0.000 claims 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- 230000003321 amplification Effects 0.000 claims 5
- 239000003153 chemical reaction reagent Substances 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 239000000872 buffer Substances 0.000 claims 4
- 150000001768 cations Chemical class 0.000 claims 4
- 238000004090 dissolution Methods 0.000 claims 3
- 239000002198 insoluble material Substances 0.000 claims 3
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 3
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 claims 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims 2
- 239000008367 deionised water Substances 0.000 claims 2
- 229910021641 deionized water Inorganic materials 0.000 claims 2
- 230000001404 mediated effect Effects 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- PJWWRFATQTVXHA-UHFFFAOYSA-N Cyclohexylaminopropanesulfonic acid Chemical compound OS(=O)(=O)CCCNC1CCCCC1 PJWWRFATQTVXHA-UHFFFAOYSA-N 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 1
- 235000011130 ammonium sulphate Nutrition 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 239000002738 chelating agent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- -1 ferric iron ions Chemical class 0.000 claims 1
- 238000009472 formulation Methods 0.000 claims 1
- 229910052742 iron Inorganic materials 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 229910001425 magnesium ion Inorganic materials 0.000 claims 1
- 244000005700 microbiome Species 0.000 claims 1
- 238000012544 monitoring process Methods 0.000 claims 1
- 239000002736 nonionic surfactant Substances 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- RVTZCBVAJQQJTK-UHFFFAOYSA-N oxygen(2-);zirconium(4+) Chemical compound [O-2].[O-2].[Zr+4] RVTZCBVAJQQJTK-UHFFFAOYSA-N 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 239000008363 phosphate buffer Substances 0.000 claims 1
- 239000001103 potassium chloride Substances 0.000 claims 1
- 235000011164 potassium chloride Nutrition 0.000 claims 1
- 229910001928 zirconium oxide Inorganic materials 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662319876P | 2016-04-08 | 2016-04-08 | |
| US62/319,876 | 2016-04-08 | ||
| PCT/US2017/023951 WO2017176469A1 (en) | 2016-04-08 | 2017-03-24 | Process for cell lysis and nucleic acid amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019517778A JP2019517778A (ja) | 2019-06-27 |
| JP2019517778A5 true JP2019517778A5 (https=) | 2020-04-30 |
| JP6979409B2 JP6979409B2 (ja) | 2021-12-15 |
Family
ID=58633077
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018552671A Active JP6979409B2 (ja) | 2016-04-08 | 2017-03-24 | 細胞溶解及び核酸増幅のためのプロセス |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US11168352B2 (https=) |
| EP (1) | EP3440223B1 (https=) |
| JP (1) | JP6979409B2 (https=) |
| CN (1) | CN109072286B (https=) |
| WO (1) | WO2017176469A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3205110A1 (en) * | 2020-12-16 | 2022-06-23 | Biofire Diagnostics, Llc | Systems, methods, and apparatuses for concentration and identification of a microorganism from blood |
| WO2023031691A1 (en) | 2021-09-01 | 2023-03-09 | 3M Innovative Properties Company | Compositions and methods of detecting analytes |
| EP4163369A1 (en) * | 2021-10-08 | 2023-04-12 | AXAGARIUS GmbH & Co. KG | Use of mixtures of polyvinylpyrrolidone and ammonium sulfate in the isolation of nucleic acids |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH056607A (ja) | 1991-06-28 | 1993-01-14 | Sony Corp | デイスクプレーヤ |
| US5712095A (en) * | 1994-06-16 | 1998-01-27 | Becton Dickinson And Company | Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors |
| JPH08149974A (ja) * | 1994-11-30 | 1996-06-11 | Q P Corp | Ｐｃｒ用増菌培地 |
| US5620869A (en) | 1995-09-28 | 1997-04-15 | Becton, Dickinson And Company | Methods for reducing inhibition of nucleic acid amplification reactions |
| WO1997044488A2 (en) * | 1996-05-22 | 1997-11-27 | Gen-Probe Incorporated | Compositions and methods for the detection of mycobacterium kansasii |
| JP5006607B2 (ja) | 2006-09-19 | 2012-08-22 | 栄研化学株式会社 | 核酸増幅反応における阻害を回避する方法 |
| US20140038174A1 (en) * | 2011-04-26 | 2014-02-06 | Longhorn Vaccines And Diagnostics, Llc | Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples |
| KR101039521B1 (ko) * | 2008-02-01 | 2011-06-08 | 주식회사 인트론바이오테크놀로지 | 금속 이온에 의해 발생하는 ｐｃｒ 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 ｐｃｒ 반응용 조성물 |
| JP2011097828A (ja) * | 2008-07-09 | 2011-05-19 | Beckman Coulter Inc | 全血試料の前処理方法及び核酸増幅方法 |
| EP2536855A4 (en) * | 2010-02-19 | 2013-07-31 | Argylla Technologies | ISOLATION OF BIOMOLECULES FROM BIOLOGICAL SAMPLES |
| EP2634254A1 (en) * | 2012-02-29 | 2013-09-04 | QIAGEN GmbH | Method for isolating nucleic acids from a food sample |
| WO2013147043A1 (ja) * | 2012-03-29 | 2013-10-03 | 株式会社シノテスト | 核酸の検出又は定量方法及び検出又は定量用試薬キット |
| EP2888363B1 (en) | 2012-08-21 | 2018-06-06 | Qiagen GmbH | Method for isolating nucleic acids from a formaldehyde releaser stabilized sample |
| EP2730653A1 (en) * | 2012-11-07 | 2014-05-14 | QIAGEN GmbH | Method for lysing a fixed biological sample |
| EP3189162A1 (en) | 2014-09-02 | 2017-07-12 | Pharmozyme, Inc. | Genetic detection platform |
| CN105462957A (zh) * | 2014-09-30 | 2016-04-06 | 鸿慧(上海)生物科技有限公司 | 一种裂解组合物及其用途、试剂盒、利用该裂解组合物制备核酸的方法、核酸分析的方法 |
| EP3237637B1 (en) * | 2014-12-23 | 2019-09-04 | 3M Innovative Properties Company | Composition for reducing inhibition of nucleic acid amplification |
| CN107980065B (zh) * | 2015-05-11 | 2021-09-07 | 3M创新有限公司 | 用于减少核酸扩增抑制的组合物 |
-
2017
- 2017-03-24 CN CN201780022472.9A patent/CN109072286B/zh active Active
- 2017-03-24 WO PCT/US2017/023951 patent/WO2017176469A1/en not_active Ceased
- 2017-03-24 JP JP2018552671A patent/JP6979409B2/ja active Active
- 2017-03-24 US US16/087,750 patent/US11168352B2/en active Active
- 2017-03-24 EP EP17719745.6A patent/EP3440223B1/en active Active
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