JP2019516371A5 - - Google Patents
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- Publication number
- JP2019516371A5 JP2019516371A5 JP2018558148A JP2018558148A JP2019516371A5 JP 2019516371 A5 JP2019516371 A5 JP 2019516371A5 JP 2018558148 A JP2018558148 A JP 2018558148A JP 2018558148 A JP2018558148 A JP 2018558148A JP 2019516371 A5 JP2019516371 A5 JP 2019516371A5
- Authority
- JP
- Japan
- Prior art keywords
- recombinant
- sequence
- nucleic acid
- host cell
- recombinant protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims description 40
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims description 40
- 150000007523 nucleic acids Chemical class 0.000 claims description 29
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 25
- 210000004027 cell Anatomy 0.000 claims description 22
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 19
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 11
- 238000012217 deletion Methods 0.000 claims description 11
- 230000037430 deletion Effects 0.000 claims description 11
- 238000003780 insertion Methods 0.000 claims description 11
- 230000037431 insertion Effects 0.000 claims description 11
- 238000006467 substitution reaction Methods 0.000 claims description 11
- 108020004707 nucleic acids Proteins 0.000 claims description 10
- 102000039446 nucleic acids Human genes 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 6
- 239000013604 expression vector Substances 0.000 claims description 6
- 238000003259 recombinant expression Methods 0.000 claims description 6
- 210000004899 c-terminal region Anatomy 0.000 claims description 5
- 230000006698 induction Effects 0.000 claims description 5
- 108020004705 Codon Proteins 0.000 claims description 4
- 239000006143 cell culture medium Substances 0.000 claims description 4
- 210000001322 periplasm Anatomy 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 230000006978 adaptation Effects 0.000 claims description 2
- 102000025171 antigen binding proteins Human genes 0.000 claims description 2
- 108091000831 antigen binding proteins Proteins 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims description 2
- 230000000813 microbial effect Effects 0.000 claims description 2
- 238000005215 recombination Methods 0.000 claims 1
- 230000006798 recombination Effects 0.000 claims 1
- 238000000746 purification Methods 0.000 description 3
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 238000011210 chromatographic step Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022015944A JP2022070902A (ja) | 2016-05-06 | 2022-02-03 | 組み換えタンパク質の生成方法 |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662332636P | 2016-05-06 | 2016-05-06 | |
| US62/332,636 | 2016-05-06 | ||
| US201662338045P | 2016-05-18 | 2016-05-18 | |
| US62/338,045 | 2016-05-18 | ||
| PCT/EP2017/060654 WO2017191255A1 (en) | 2016-05-06 | 2017-05-04 | Method of producing a recombinant protein |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022015944A Division JP2022070902A (ja) | 2016-05-06 | 2022-02-03 | 組み換えタンパク質の生成方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2019516371A JP2019516371A (ja) | 2019-06-20 |
| JP2019516371A5 true JP2019516371A5 (cg-RX-API-DMAC10.html) | 2020-05-28 |
Family
ID=58765814
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018558148A Pending JP2019516371A (ja) | 2016-05-06 | 2017-05-04 | 組み換えタンパク質の生成方法 |
| JP2022015944A Pending JP2022070902A (ja) | 2016-05-06 | 2022-02-03 | 組み換えタンパク質の生成方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022015944A Pending JP2022070902A (ja) | 2016-05-06 | 2022-02-03 | 組み換えタンパク質の生成方法 |
Country Status (14)
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020165874A1 (en) * | 2019-02-15 | 2020-08-20 | Oncosimis Biotech Private Limited | A bacterial expression vector for enhanced protein secretion |
| KR102094480B1 (ko) | 2019-05-27 | 2020-04-01 | (주)엘에스케이화인텍스 | 재사용 가능한 미세 먼지 항균 마스크 |
| CN110551199B (zh) * | 2019-09-26 | 2022-01-25 | 杭州纽龙日尚生物制品有限公司 | 一种提高工程菌分泌表达重组人表皮生长因子的方法 |
| KR20240021234A (ko) | 2021-06-10 | 2024-02-16 | 펠리칸 테크놀로지 홀딩스, 인크. | 재조합 단백질 발현을 위한 박테리아 숙주 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1546342A2 (en) * | 2002-09-20 | 2005-06-29 | Cym1P A/S | Methods for increasing the production of a recombinant polypeptide from a host cell |
| GB0426160D0 (en) * | 2004-11-29 | 2004-12-29 | Era Plantech S L | Production of proteins |
| KR100981356B1 (ko) * | 2006-01-31 | 2010-09-14 | 대한민국 | 신호서열 및 변이된 신호서열로 디자인된 분비증강자에의한 원래 형태의 수용성 재조합 단백질 생산 방법 |
| GB0602173D0 (en) | 2006-02-03 | 2006-03-15 | Avecia Ltd | Expression system |
| WO2009021548A1 (en) * | 2007-08-10 | 2009-02-19 | Wacker Chemie Ag | Expression of full length igg and secretion into the culture medium of prokaryotic cells |
| RU2488635C1 (ru) * | 2012-03-22 | 2013-07-27 | Государственное бюджетное образовательное учреждение высшего профессионального образования Первый Московский государственный медицинский университет им. И.М. Сеченова Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО Первый МГМУ им. И.М. Сеченова Минздравсоцразвития | Способ получения рекомбинантного антиангиогенного полипептида |
| US20140244228A1 (en) * | 2012-09-19 | 2014-08-28 | Agency For Science, Technology And Research | Codon optimization of a synthetic gene(s) for protein expression |
-
2017
- 2017-05-04 CN CN201780028031.XA patent/CN109071611B/zh not_active Expired - Fee Related
- 2017-05-04 RU RU2018137305A patent/RU2742006C2/ru active
- 2017-05-04 SG SG11201808976WA patent/SG11201808976WA/en unknown
- 2017-05-04 MX MX2018013562A patent/MX2018013562A/es unknown
- 2017-05-04 WO PCT/EP2017/060654 patent/WO2017191255A1/en not_active Ceased
- 2017-05-04 CA CA3023025A patent/CA3023025A1/en not_active Abandoned
- 2017-05-04 BR BR112018072043-7A patent/BR112018072043A2/pt not_active Application Discontinuation
- 2017-05-04 TW TW106114729A patent/TWI794170B/zh active
- 2017-05-04 JP JP2018558148A patent/JP2019516371A/ja active Pending
- 2017-05-04 AU AU2017260277A patent/AU2017260277A1/en not_active Abandoned
- 2017-05-04 US US16/098,890 patent/US11098312B2/en active Active
- 2017-05-04 KR KR1020187034736A patent/KR102504317B1/ko active Active
- 2017-05-04 EP EP17725188.1A patent/EP3452496A1/en not_active Withdrawn
-
2018
- 2018-10-24 IL IL262544A patent/IL262544A/en unknown
-
2022
- 2022-02-03 JP JP2022015944A patent/JP2022070902A/ja active Pending
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