JP2019500324A5 - - Google Patents

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Publication number
JP2019500324A5
JP2019500324A5 JP2018522968A JP2018522968A JP2019500324A5 JP 2019500324 A5 JP2019500324 A5 JP 2019500324A5 JP 2018522968 A JP2018522968 A JP 2018522968A JP 2018522968 A JP2018522968 A JP 2018522968A JP 2019500324 A5 JP2019500324 A5 JP 2019500324A5
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JP
Japan
Prior art keywords
group
disulfide
deoxynucleoside triphosphate
labeled
deoxynucleoside
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JP2018522968A
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English (en)
Japanese (ja)
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JP2019500324A (ja
JP6775014B2 (ja
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Priority claimed from PCT/US2016/060435 external-priority patent/WO2017079498A2/en
Publication of JP2019500324A publication Critical patent/JP2019500324A/ja
Publication of JP2019500324A5 publication Critical patent/JP2019500324A5/ja
Application granted granted Critical
Publication of JP6775014B2 publication Critical patent/JP6775014B2/ja
Expired - Fee Related legal-status Critical Current
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JP2018522968A 2015-11-06 2016-11-04 ヌクレオチド類似体 Expired - Fee Related JP6775014B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562251884P 2015-11-06 2015-11-06
US62/251,884 2015-11-06
US201662327555P 2016-04-26 2016-04-26
US62/327,555 2016-04-26
PCT/US2016/060435 WO2017079498A2 (en) 2015-11-06 2016-11-04 Nucleotide analogues

Related Child Applications (1)

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JP2020168502A Division JP7119041B2 (ja) 2015-11-06 2020-10-05 Ｄｎａ配列において標識されたヌクレオチドを検出する方法

Publications (3)

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JP2019500324A JP2019500324A (ja) 2019-01-10
JP2019500324A5 true JP2019500324A5 (enExample) 2019-08-08
JP6775014B2 JP6775014B2 (ja) 2020-10-28

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JP2018522968A Expired - Fee Related JP6775014B2 (ja) 2015-11-06 2016-11-04 ヌクレオチド類似体
JP2020168502A Active JP7119041B2 (ja) 2015-11-06 2020-10-05 Ｄｎａ配列において標識されたヌクレオチドを検出する方法

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JP2020168502A Active JP7119041B2 (ja) 2015-11-06 2020-10-05 Ｄｎａ配列において標識されたヌクレオチドを検出する方法

Country Status (9)

Country Link
US (7) US10273539B2 (enExample)
EP (1) EP3370731A4 (enExample)
JP (2) JP6775014B2 (enExample)
KR (2) KR102298737B1 (enExample)
CN (1) CN109476694B (enExample)
AU (1) AU2016349457B2 (enExample)
CA (2) CA3158679A1 (enExample)
TW (3) TWI639614B (enExample)
WO (1) WO2017079498A2 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3356381A4 (en) 2015-09-28 2019-06-12 The Trustees of Columbia University in the City of New York DESIGN AND SYNTHESIS OF NUCLEOTIDES BASED ON NOVEL DISULFIDE COMPOUNDS AS REVERSIBLE TERMINATORS FOR DNA SEQUENCING BY SYNTHESIS
TWI639614B (zh) * 2015-11-06 2018-11-01 美商齊亞雋科學公司 核苷酸類似物
US11266673B2 (en) 2016-05-23 2022-03-08 The Trustees Of Columbia University In The City Of New York Nucleotide derivatives and methods of use thereof
WO2018129644A1 (zh) * 2017-01-10 2018-07-19 深圳华大智造科技有限公司 一种提高核酸聚合测序质量的方法、反应体系和试剂盒
EP3580351A1 (en) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymerase enzyme from 9°n
US11591647B2 (en) 2017-03-06 2023-02-28 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (SBS) methods that combine SBS cycle steps
CN119639879A (zh) 2017-03-28 2025-03-18 哥伦比亚大学董事会 用于连接荧光标记与用于dna合成测序的碱基的具有不同可裂解连接子的3'-o-改性的核苷酸类似物
WO2019105421A1 (zh) 2017-11-30 2019-06-06 深圳市瀚海基因生物科技有限公司 核苷类似物、制备方法及应用
US12215124B2 (en) 2018-02-21 2025-02-04 Singular Genomics Systems, Inc. Fluorinated nucleotides and uses thereof
WO2019178393A1 (en) 2018-03-15 2019-09-19 The Trustees Of Columbia University In The City Of New York Nucleotide analogues and use thereof for nucleic acid sequencing and analysis
WO2020056044A1 (en) 2018-09-11 2020-03-19 Singular Genomics Systems, Inc. Modified archaeal family b polymerases
WO2020069424A1 (en) 2018-09-28 2020-04-02 Centrillion Technologies, Inc. Disulfide-linked reversible terminators
EP3870593A4 (en) 2018-10-25 2022-11-16 Singular Genomics Systems, Inc. NUCLEOTIDE ANALOGUES
CN112888699B (zh) * 2018-11-20 2024-05-17 深圳华大生命科学研究院 测序用核苷酸的制备方法
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
JP2022516684A (ja) * 2019-01-08 2022-03-01 シンギュラー・ゲノミクス・システムズ・インコーポレイテッド ヌクレオチドの開裂可能なリンカーおよびその使用
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
WO2021067970A1 (en) * 2019-10-04 2021-04-08 Centrillion Technologies, Inc. Reversible terminators for dna sequencing and methods of using the same
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
US11174281B1 (en) 2020-04-24 2021-11-16 Singular Genomics Systems, Inc. Modified nucleotides and uses thereof
WO2021226327A1 (en) * 2020-05-08 2021-11-11 Singular Genomics Systems, Inc. Nucleotide cleavable linkers with rigid spacers and uses thereof
CN115867560B (zh) * 2020-06-22 2025-03-28 伊鲁米纳剑桥有限公司 具有3’缩醛封端基团的核苷与核苷酸
US12031179B2 (en) 2020-10-30 2024-07-09 Singular Genomics Systems, Inc. Methods and compositions for reducing nucleotide impurities
EP4036098A1 (en) 2021-02-02 2022-08-03 Miltenyi Biotec B.V. & Co. KG Synthesis of reversible nucleotide terminators by in-situ thio-alkyl group transfer for use in dna sequencing by synthesis
EP4317168A4 (en) * 2021-04-01 2025-08-27 Bgi Shenzhen NUCLEOTIDE ANALOGUE FOR SEQUENCING
EP4089098A1 (en) 2021-05-10 2022-11-16 Miltenyi Biotec B.V. & Co. KG Fret dye labeled reversible nucleotide terminators and their use in sequencing of dna
CN113341058A (zh) * 2021-06-09 2021-09-03 南京溯远基因科技有限公司 一种三色高通量测序试剂及测序方法
WO2023107673A2 (en) * 2021-12-10 2023-06-15 Singular Genomics Systems, Inc. Cleavable disulfide linkers and uses thereof
JP2025505253A (ja) 2022-02-11 2025-02-21 ミルテニー バイオテック ベー．フェー． ウント コー． カー・ゲー 酵素的ｄｎａおよびｒｎａ合成のための３’－オキシメチレンアルキルジスルフィド保護ヌクレオチドの使用
JP2025510104A (ja) * 2022-03-22 2025-04-14 デクソーム カンパニー， リミテッド Ｌ－ポリヌクレオチドの配列決定方法
WO2023192815A2 (en) * 2022-03-28 2023-10-05 Baylor College Of Medicine Taxonomic signatures and methods of determining the same
CN115417907A (zh) * 2022-08-15 2022-12-02 上海交通大学医学院 一种连接体及其制备方法
EP4342998A1 (en) 2022-09-23 2024-03-27 Miltenyi Biotec B.V. & Co. KG Labeled probes with differentially cleavable linkers and their use in de-coding dna and rna molecules
CN115960152A (zh) * 2022-10-11 2023-04-14 河南师范大学 一种炔丙醚基取代tpt3型非天然碱基三磷酸及其合成方法和应用
CN115785170A (zh) * 2022-12-02 2023-03-14 重庆铭毅智造科技有限公司 一种可逆终止子核苷酸3’-oh保护基团的引入方法
WO2025072834A2 (en) * 2023-09-27 2025-04-03 Dxome Co., Ltd. Method of sequencing and synthesizing l-polynucleotide

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO873956L (no) 1986-09-25 1988-03-28 Bp Chem Int Ltd Rensemidler inneholdende peroksyder for fremstilling av flyktige sulfider.
US5756334A (en) 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
US5633360A (en) * 1992-04-14 1997-05-27 Gilead Sciences, Inc. Oligonucleotide analogs capable of passive cell membrane permeation
US5990300A (en) 1994-09-02 1999-11-23 Andrew C. Hiatt Enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US6046039A (en) 1998-08-19 2000-04-04 Battelle Memorial Institute Methods for producing partially digested restriction DNA fragments and for producing a partially modified PCR product
US6465628B1 (en) 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
AU2002301870A1 (en) * 1999-03-12 2003-03-13 President And Fellows Of Harvard College Nuclotide Compounds Having a Cleavable Linker
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
US6498189B1 (en) 1999-11-10 2002-12-24 University Of New Mexico Method for induction of L-selectin shedding
WO2002029003A2 (en) 2000-10-06 2002-04-11 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
GB0129012D0 (en) * 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) * 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US20040235027A1 (en) * 2003-03-31 2004-11-25 Regent Of The University Of California Preparation and application of ligand-biopolymer conjugates
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US20070117104A1 (en) 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
GB2446083B (en) 2005-10-31 2011-03-02 Univ Columbia Chemically cleavable 3'-0-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
WO2008069973A2 (en) 2006-12-01 2008-06-12 The Trustees Of Columbia University In The City Of New York Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US8481259B2 (en) * 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
EP3431615A3 (en) * 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna sequencing with non-fluorescent nucleotide reversible terminators cleavable label modified nucleotide terminators
US8017338B2 (en) 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
US8101353B2 (en) 2007-12-18 2012-01-24 Advanced Analytical Technologies, Inc. System and method for nucleotide sequence profiling for sample identification
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US20100035253A1 (en) * 2008-03-19 2010-02-11 Intelligent Bio-Systems, Inc. Methods And Compositions For Incorporating Nucleotides
US20100330569A1 (en) 2009-04-23 2010-12-30 Intelligent Bio-Systems, Inc. Hydroxymethyl Linkers For Labeling Nucleotides
EP3670523B1 (en) * 2011-09-13 2021-08-11 Agilent Technologies, Inc. 3'-oh unblocked, fast photocleavable terminating nucleotides and their use in methods for nucleic acid sequencing
CN103484106B (zh) * 2013-09-05 2015-08-19 上海交通大学 四色荧光标记可逆终端及其在dna测序中的用途
CA2929651A1 (en) * 2013-11-06 2015-05-14 Solstice Biologics, Ltd. Polynucleotide constructs having disulfide groups
EP2876166B1 (en) * 2013-11-20 2016-12-14 Roche Diagnostics GmbH New compound for sequencing by synthesis
EP3356381A4 (en) * 2015-09-28 2019-06-12 The Trustees of Columbia University in the City of New York DESIGN AND SYNTHESIS OF NUCLEOTIDES BASED ON NOVEL DISULFIDE COMPOUNDS AS REVERSIBLE TERMINATORS FOR DNA SEQUENCING BY SYNTHESIS
TWI639614B (zh) * 2015-11-06 2018-11-01 美商齊亞雋科學公司 核苷酸類似物

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