KR102298737B1 - 뉴클레오티드 유사체 - Google Patents
뉴클레오티드 유사체 Download PDFInfo
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- KR102298737B1 KR102298737B1 KR1020187015618A KR20187015618A KR102298737B1 KR 102298737 B1 KR102298737 B1 KR 102298737B1 KR 1020187015618 A KR1020187015618 A KR 1020187015618A KR 20187015618 A KR20187015618 A KR 20187015618A KR 102298737 B1 KR102298737 B1 KR 102298737B1
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- deoxynucleoside triphosphate
- compound
- labeled
- disulfide
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- 0 CCOC(C1)[C@@](*OP(O)(OP(O)(OP(O)(O)=O)=O)=O)O*1N(C=C(C(N)=N1)C#CNC(OCCCCOCS[S+]C(C)(C)CCOC(NCCOCCOCCNC(c(cc2)cc(C(c(c(O3)c4S(O)(=O)=O)ccc4N)=C(C=CC4=[N+])C3=C4S(O)(=O)=O)c2C(O)=O)=O)=O)=O)C1=O Chemical compound CCOC(C1)[C@@](*OP(O)(OP(O)(OP(O)(O)=O)=O)=O)O*1N(C=C(C(N)=N1)C#CNC(OCCCCOCS[S+]C(C)(C)CCOC(NCCOCCOCCNC(c(cc2)cc(C(c(c(O3)c4S(O)(=O)=O)ccc4N)=C(C=CC4=[N+])C3=C4S(O)(=O)=O)c2C(O)=O)=O)=O)=O)C1=O 0.000 description 6
- JORBPJNOFFEITQ-UHFFFAOYSA-N CC(C)(CCOC(NCCOCCOCCNC(OCC1c2ccccc2-c2c1cccc2)=O)=O)SSCOCCCCOC(ON(C(CC1)=O)C1=O)=O Chemical compound CC(C)(CCOC(NCCOCCOCCNC(OCC1c2ccccc2-c2c1cccc2)=O)=O)SSCOCCCCOC(ON(C(CC1)=O)C1=O)=O JORBPJNOFFEITQ-UHFFFAOYSA-N 0.000 description 1
- FHGKFVXTGWEHJZ-UHFFFAOYSA-N CCOCSSc1ccccc1 Chemical compound CCOCSSc1ccccc1 FHGKFVXTGWEHJZ-UHFFFAOYSA-N 0.000 description 1
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- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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- C07C323/10—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton
- C07C323/11—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
- C07C323/12—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being acyclic and saturated
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- C07C323/50—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton
- C07C323/51—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
- C07C323/60—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton with the carbon atom of at least one of the carboxyl groups bound to nitrogen atoms
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- C07D207/02—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D207/30—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members
- C07D207/34—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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- C07F7/00—Compounds containing elements of Groups 4 or 14 of the Periodic Table
- C07F7/02—Silicon compounds
- C07F7/08—Compounds having one or more C—Si linkages
- C07F7/18—Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
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- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/14—Pyrrolo-pyrimidine radicals
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- C09B11/00—Diaryl- or thriarylmethane dyes
- C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
- C09B11/10—Amino derivatives of triarylmethanes
- C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
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- C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
- C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
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- C09B23/16—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing hetero atoms
- C09B23/162—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing hetero atoms only nitrogen atoms
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- C09B5/00—Dyes with an anthracene nucleus condensed with one or more heterocyclic rings with or without carbocyclic rings
- C09B5/24—Dyes with an anthracene nucleus condensed with one or more heterocyclic rings with or without carbocyclic rings the heterocyclic rings being only condensed with an anthraquinone nucleus in 1-2 or 2-3 position
- C09B5/2409—Dyes with an anthracene nucleus condensed with one or more heterocyclic rings with or without carbocyclic rings the heterocyclic rings being only condensed with an anthraquinone nucleus in 1-2 or 2-3 position not provided for in one of the sub groups C09B5/26 - C09B5/62
- C09B5/2436—Dyes with an anthracene nucleus condensed with one or more heterocyclic rings with or without carbocyclic rings the heterocyclic rings being only condensed with an anthraquinone nucleus in 1-2 or 2-3 position not provided for in one of the sub groups C09B5/26 - C09B5/62 only nitrogen-containing hetero rings
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
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- C07C2603/04—Ortho- or ortho- and peri-condensed systems containing three rings
- C07C2603/06—Ortho- or ortho- and peri-condensed systems containing three rings containing at least one ring with less than six ring members
- C07C2603/10—Ortho- or ortho- and peri-condensed systems containing three rings containing at least one ring with less than six ring members containing five-membered rings
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020217027925A KR102454782B1 (ko) | 2015-11-06 | 2016-11-04 | 뉴클레오티드 유사체 |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562251884P | 2015-11-06 | 2015-11-06 | |
| US62/251,884 | 2015-11-06 | ||
| US201662327555P | 2016-04-26 | 2016-04-26 | |
| US62/327,555 | 2016-04-26 | ||
| PCT/US2016/060435 WO2017079498A2 (en) | 2015-11-06 | 2016-11-04 | Nucleotide analogues |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020217027925A Division KR102454782B1 (ko) | 2015-11-06 | 2016-11-04 | 뉴클레오티드 유사체 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20180080280A KR20180080280A (ko) | 2018-07-11 |
| KR102298737B1 true KR102298737B1 (ko) | 2021-09-08 |
Family
ID=58663032
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020217027925A Active KR102454782B1 (ko) | 2015-11-06 | 2016-11-04 | 뉴클레오티드 유사체 |
| KR1020187015618A Expired - Fee Related KR102298737B1 (ko) | 2015-11-06 | 2016-11-04 | 뉴클레오티드 유사체 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020217027925A Active KR102454782B1 (ko) | 2015-11-06 | 2016-11-04 | 뉴클레오티드 유사체 |
Country Status (9)
| Country | Link |
|---|---|
| US (7) | US10336785B2 (enExample) |
| EP (1) | EP3370731A4 (enExample) |
| JP (2) | JP6775014B2 (enExample) |
| KR (2) | KR102454782B1 (enExample) |
| CN (1) | CN109476694B (enExample) |
| AU (1) | AU2016349457B2 (enExample) |
| CA (2) | CA3004060C (enExample) |
| TW (3) | TWI639614B (enExample) |
| WO (1) | WO2017079498A2 (enExample) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3356381A4 (en) * | 2015-09-28 | 2019-06-12 | The Trustees of Columbia University in the City of New York | DESIGN AND SYNTHESIS OF NUCLEOTIDES BASED ON NOVEL DISULFIDE COMPOUNDS AS REVERSIBLE TERMINATORS FOR DNA SEQUENCING BY SYNTHESIS |
| WO2017079498A2 (en) * | 2015-11-06 | 2017-05-11 | Intelligent Biosystems, Inc. | Nucleotide analogues |
| EP3463381A4 (en) * | 2016-05-23 | 2020-01-01 | The Trustees of Columbia University in the City of New York | NUCLEOTIDE DERIVATIVES AND THEIR METHODS OF USE |
| WO2018129644A1 (zh) * | 2017-01-10 | 2018-07-19 | 深圳华大智造科技有限公司 | 一种提高核酸聚合测序质量的方法、反应体系和试剂盒 |
| EP3580351A1 (en) | 2017-02-13 | 2019-12-18 | Qiagen Sciences, LLC | Polymerase enzyme from 9°n |
| EP3596099B1 (en) | 2017-03-06 | 2024-07-24 | Singular Genomics Systems, Inc. | Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps |
| CN110709089B (zh) | 2017-03-28 | 2024-12-06 | 哥伦比亚大学董事会 | 用于连接荧光标记与用于dna合成测序的碱基的具有不同可裂解连接子的3’-o-改性的核苷酸类似物 |
| CN111741967A (zh) | 2017-11-30 | 2020-10-02 | 深圳市真迈生物科技有限公司 | 核苷类似物、制备方法及应用 |
| WO2019164977A1 (en) | 2018-02-21 | 2019-08-29 | Singular Genomics Systems, Inc. | Fluorinated nucleotides and uses thereof |
| EP3765028A4 (en) | 2018-03-15 | 2021-12-15 | The Trustees of Columbia University in the City of New York | NUCLEOTID ANALOGUES AND THEIR USE FOR NUCLEIC ACID SEQUENCING AND ANALYSIS |
| US20230011240A1 (en) | 2018-09-11 | 2023-01-12 | Singular Genomics Systems, Inc. | Modified archaeal family b polymerases |
| EP3856753A4 (en) * | 2018-09-28 | 2022-11-16 | Centrillion Technology Holdings Corporation | DISULFIDE CROSSLINKED REVERSIBLE TERMINATORS |
| WO2020086834A1 (en) | 2018-10-25 | 2020-04-30 | Singular Genomics Systems, Inc. | Nucleotide analogues |
| CN112888699B (zh) * | 2018-11-20 | 2024-05-17 | 深圳华大生命科学研究院 | 测序用核苷酸的制备方法 |
| US11293061B2 (en) | 2018-12-26 | 2022-04-05 | Illumina Cambridge Limited | Sequencing methods using nucleotides with 3′ AOM blocking group |
| CN113453692A (zh) * | 2019-01-08 | 2021-09-28 | 奇异基因组学系统公司 | 核苷酸可裂解接头和其用途 |
| US11512295B2 (en) | 2019-09-12 | 2022-11-29 | Singular Genomics Systems, Inc. | Modified thermoccocus polymerases |
| WO2021067970A1 (en) * | 2019-10-04 | 2021-04-08 | Centrillion Technologies, Inc. | Reversible terminators for dna sequencing and methods of using the same |
| US11034942B1 (en) | 2020-02-27 | 2021-06-15 | Singular Genomics Systems, Inc. | Modified pyrococcus polymerases and uses thereof |
| US11174281B1 (en) | 2020-04-24 | 2021-11-16 | Singular Genomics Systems, Inc. | Modified nucleotides and uses thereof |
| WO2021226327A1 (en) * | 2020-05-08 | 2021-11-11 | Singular Genomics Systems, Inc. | Nucleotide cleavable linkers with rigid spacers and uses thereof |
| MX2022016492A (es) * | 2020-06-22 | 2023-03-06 | Illumina Cambridge Ltd | Nucleosidos y nucleotidos con grupo de bloqueo de acetal 3'. |
| US12031179B2 (en) | 2020-10-30 | 2024-07-09 | Singular Genomics Systems, Inc. | Methods and compositions for reducing nucleotide impurities |
| EP4036098A1 (en) * | 2021-02-02 | 2022-08-03 | Miltenyi Biotec B.V. & Co. KG | Synthesis of reversible nucleotide terminators by in-situ thio-alkyl group transfer for use in dna sequencing by synthesis |
| CN115197289B (zh) * | 2021-04-01 | 2025-03-07 | 深圳华大生命科学研究院 | 用于测序的核苷酸类似物 |
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