JP2019170161A - Production method of ganoderma lucidum, antioxidant, medicine, food product and skin external preparation - Google Patents

Abstract

To provide production methods of Ganoderma lucidum, antioxidant, medicine, food product, and skin external preparation capable of increasing yield of Ganoderma lucidum and increasing efficacy or active principle.SOLUTION: A production method of Ganoderma lucidum uses a plasma generator 100. The plasma generator 100 comprises a first electrode body 110 and a second electrode body 120. The first electrode 110 comprises a first electrode 111 and a dielectric body 112. The second electrode body 120 has a second electrode 121. In a state of not bringing the first electrode 111 of the plasma generator 100 into direct contact with a culture raw wood or culture mushroom bed, the plasma is radiated to the culture raw wood or culture mushroom bed.SELECTED DRAWING: Figure 3

Description

  The technical field of the present specification relates to a method for producing garlic mushrooms, antioxidants, pharmaceuticals, foods, and skin external preparations.

  Mannentake is a basidiomycete used in the herbal medicine “Reishi”. Mannentake has a wide range of physiological activities and is widely used as a Chinese medicine. Mannentake contains active ingredients such as polysaccharides such as β-glucan and triterpenes such as ganoderic acid. β-glucan is known to have an anticancer effect, an immunostimulatory effect, and the like. In addition, ganoderic acid has been reported to have many effects such as an anticancer effect, a blood pressure lowering effect, and a cholesterol lowering effect. Moreover, as an extract of Mannentake, medicinal effects such as a moisturizing action, a cell growth promoting action, a whitening action, and a collagen synthesis promoting action are known.

  At present, most of the mannambotes that are distributed are artificially cultivated. As the cultivation method, mainly two methods of raw wood cultivation and fungus bed cultivation are used. Various cultivation methods have been developed for the purpose of increasing the yield and the active ingredient of garlic mushroom. For example, Patent Literature 1 discloses a method for cultivating garlic mushrooms that uses chlorella hot water extraction residue or dried coconut mix as a medium component (Patent Literature 1).

JP 2014-158441 A JP 2012-54 A

  By the way, in general cultivation of straw, a technique for applying electrical stimulation to a bed tree has been developed. For example, Patent Literature 2 discloses a technique in which a cultivator-side electrode 1a is inserted into a tree and a spark discharge is generated between the cultivator-side electrode 1a and the discharge electrode 20 (see, for example, FIG. 1 of Patent Literature 2). ). Thereby, it is disclosed that the yield of straw is increased (see paragraph [0024] of Patent Document 2).

  However, unlike edible salmon, the active ingredient extracted from Mannentake is used as the above-mentioned medicinal effect. Therefore, when producing garlic mushrooms, an increase in active ingredients is required as the yield increases. The active ingredient is, for example, ganoderic acid having an anticancer action, a blood pressure lowering action, and a cholesterol lowering action. Moreover, it is preferable that an antioxidant effect | action increases as an effect of a bamboo shoot.

  The technique of this specification has been made to solve the problems of the conventional techniques described above. The problem is to provide a method for producing ganentake, antioxidants, pharmaceuticals, foods, and external preparations for skin, which can increase the yield of garlic mushroom and increase the efficacy or active ingredient.

  In the method for producing mannentake in the first aspect, a plasma generator having a first electrode body including a first conductor is used. Plasma is irradiated to the culture log or the culture fungus in a state where the first conductor of the plasma generator is not in direct contact with the culture log or the culture fungus bed.

  In this production method, the yield of garlic mushrooms is larger than the conventional amount. The extract of Mannentake obtained by this production method exhibits a higher antioxidant effect than the extract of Mannentake. In addition, the content of ganoderic acid contained in the garlic mushroom obtained by this production method is higher than the content of ganoderic acid contained in the conventional garlic mushroom. As described above, this method for producing mushrooms increases the yield or the active ingredient at the same time.

  In the present specification, there is provided a method for producing garlic mushrooms, antioxidants, pharmaceuticals, foods, and external preparations for skin, which can increase the yield of garlic mushrooms and increase the efficacy or active ingredient.

It is a schematic block diagram which shows the structure of the 1st plasma generator of 1st Embodiment. It is a schematic block diagram which shows the structure of the 2nd plasma generator of 1st Embodiment. It is a figure which shows a mode that plasma is irradiated to a microbial bed using the 1st plasma generator in 1st this embodiment. It is FIG. (1) which shows a mode that plasma is irradiated to a microbial bed using the 2nd plasma generator in 1st Embodiment. It is a figure (the 2) which shows a mode that plasma is irradiated to a microbial bed using the 2nd plasma generator in 1st Embodiment. It is a graph which shows the HPLC chromatogram of a reishi extract.

  Hereinafter, specific embodiments will be described with reference to the drawings, taking as an example the production method of garlic mushrooms, antioxidants, pharmaceuticals, foods, and skin external preparations. An extract of the fruit body of the garlic bamboo contains one or more components. In the embodiments and examples, the components of the extract of the garlic mushroom may be simply referred to as components.

(First embodiment)
A first embodiment will be described. The first embodiment is a method for producing mannentake. The method for producing mannentake of this embodiment is a method for producing mannentake using a plasma generator.

1. Mannentake The strain of Mannentake of the present embodiment is a basidiomycete used in the herbal medicine “Ganoderma” and belongs to the family Ganodermaaceae and Ganoderma. For the mushrooms of the genus Mannenchu, there are old Chinese pharmacy books, “Honcho Tsuname” and “Shinnohonhonsyo”. (Aoshiba), Yellow Reishi (Koshiba) and White Reishi (White Shiba) are described as being present. Kazuno Ganoderma is also a kind of red ganoderma. The scientific names of red ganoderma and deer ganoderma are (Ganoderma lucidum). The scientific name of Black Ganoderma is (G.atrum, G.japonicum, G.sinense).

  As a strain of Mannentake, those distributed in China and the Japanese market can be used. Further, it may be produced by separating and culturing from a tissue of garlic mushroom.

2. Plasma generator 2-1. First plasma generator 2-1-1. Structure of First Plasma Generator FIG. 1 is a schematic configuration diagram showing a structure of a first plasma generator 100 of the present embodiment. The first plasma generator 100 generates atmospheric pressure plasma by dielectric barrier discharge. The first plasma generator 100 generates plasma between the object O1 disposed between the two electrode bodies. The object O1 is preferably a conductor. Or it is preferable to have a certain amount of conductivity. The object O1 may have a thin insulating layer such as a plastic bag outside.

  As shown in FIG. 1, the first plasma generation apparatus 100 includes a first electrode body 110, a second electrode body 120, and a voltage application unit 130. The first electrode body 110 includes a first electrode 111 and a dielectric 112. The first electrode 111 is a first conductor. The first electrode 111 is, for example, a metal. Examples of the material of the first electrode 111 include copper and aluminum. Of course, other metals or alloys may be used. The first electrode 111 is a flat plate electrode.

  The dielectric 112 has a flat plate shape. The dielectric 112 is in contact with the first electrode 111. The dielectric 112 is provided on the surface 111 a of the first electrode 111 on the side facing the second electrode 121. That is, the dielectric 112 covers the surface of the first electrode 111. The dielectric 112 has a surface 112a. Examples of the dielectric include quartz glass and ceramics. Of course, other dielectric materials may be used.

  The second electrode body 120 has a second electrode 121. The second electrode 121 is a second conductor. The second electrode 121 is, for example, a metal. Examples of the material of the second electrode 121 include copper and aluminum. Of course, other metals or alloys may be used. The second electrode 121 is a flat plate electrode. The second electrode 121 has a surface 121 a on the side facing the first electrode body 110.

  The area of the plate shape of the dielectric 112 is larger than the area of the plate shape of the first electrode 111. Therefore, when viewed from the second electrode 121, the surface 111 a of the first electrode 111 is hidden behind the dielectric 112.

  The first electrode body 110 and the second electrode body 120 are counter electrodes facing each other. The surface 112a of the dielectric 112 of the first electrode body 110 and the surface 121a of the second electrode 121 of the second electrode body 120 face each other. Further, the surface 111 a of the first electrode 111 of the first electrode body 110 and the surface 121 a of the second electrode 121 of the second electrode body 120 face each other.

2-1-2. Plasma Emission Region of First Plasma Generator As shown in FIG. 1, the voltage application unit 130 applies an alternating voltage between the first electrode 111 and the second electrode 121. Here, the AC voltage is, for example, 1 kV or more and 10 kV or less. The frequency is 5 kHz or more and 100 kHz or less. These are examples, and numerical values other than these may be used. Since the object O1 is a conductor, the second electrode 121 and the object O1 have almost the same potential. On the other hand, a dielectric 112 is disposed between the first electrode 111 and the object O1. That is, the first electrode 111 and the object O1 are insulated. A strong electric field is formed in the space between the first electrode 111 and the object O1. When the voltage between the first electrode 111 and the object O1 generated by the electric field exceeds the discharge start voltage, a discharge is generated between the dielectric 112 and the object O1. Then, plasma is generated in the plasma emission region P1. The plasma emission region P1 is a region that emits light by generating plasma.

  That is, as shown in FIG. 1, the first plasma generating apparatus 100 generates a plasma emission region P1 at a position between the dielectric 112 and the object O1. The first electrode 111 and the second electrode 121 are plate electrodes. Then, due to the voltage applied by the voltage application unit 130, discharge occurs in various places in the region where the dielectric 112 and the object O1 face each other. Therefore, the shape of the plasma light emitting region P <b> 1 has a planar shape corresponding to the flat plate shape of the dielectric 112. The plasma in the plasma emission region P1 is generated substantially uniformly on the upper surface O1a of the object O1. Further, the plasma light emitting region P <b> 1 is located in a space sandwiched between the first electrode body 110 and the second electrode body 120.

2-2. Second plasma generator 2-2-1. Structure of Second Plasma Generator FIG. 2 is a schematic configuration diagram showing the structure of the second plasma generator 200 of the present embodiment. The second plasma generator 200 generates atmospheric pressure plasma by dielectric barrier discharge. The second plasma generation apparatus 200 irradiates the object O1 with plasma generated by discharging between the two electrode bodies. When using the second plasma generator 200, the conductivity of the object O1 is not required.

  As shown in FIG. 2, the second plasma generator 200 includes a housing 201, a first electrode body 210, a second electrode body 220, a voltage application section 230, a gas supply section 240, and an irradiation port 201a. And having.

  The first electrode body 210 includes a first electrode 211 and a dielectric 212. The first electrode 211 is a conductor. The first electrode 211 is, for example, a metal. The first electrode 211 has a cylindrical shape. The dielectric 212 is also cylindrical. The dielectric 212 is disposed inside the first electrode 211.

  The second electrode body 220 has a second electrode 221. The second electrode 221 is a rod-shaped electrode. In addition, the dielectric 212 is disposed at a position between the first electrode 211 and the second electrode 221.

  The gas supply unit 240 supplies gas into the housing 201. Therefore, the gas supplied by the gas supply unit 240 is supplied between the first electrode body 210 and the second electrode body 220. The gas supplied by the gas supply unit 240 is a rare gas such as Ar or He. Or it may be the atmosphere. When the gas supply unit 240 supplies a rare gas such as Ar, the second plasma generator 200 easily generates plasma.

2-2-2. Plasma Emission Area of Second Plasma Generator As shown in FIG. 2, the voltage application unit 230 applies an AC voltage between the first electrode 211 and the second electrode 221. A strong electric field is formed in the space between the first electrode 211 and the second electrode 221. When the voltage between the first electrode 211 and the second electrode 221 generated by this electric field exceeds the discharge start voltage, a discharge is generated between the dielectric 212 and the second electrode 221. Then, plasma is generated in the discharge region.

  Since the gas supply unit 240 supplies gas to the inside of the casing 201 of the second plasma generator 200, the plasma is irradiated from the irradiation port 201a. Then, as shown in FIG. 2, a plasma emission region P2 is generated outside the irradiation port 201a. The plasma emission region P2 is a region that emits light by generating plasma.

3. 3. Method of plasma irradiation to log or fungus bed 3-1. First Plasma Generating Device FIG. 3 is a diagram showing a state in which plasma is applied to the fungus bed using the first plasma generating device 100 in the present embodiment. The fungus bed B1 has an upper surface B1a. The upper surface B1a has an uneven shape. In FIG. 3, the concavo-convex shape of the upper surface B1a is drawn larger for explanation. In FIG. 3, the fungus bed B1 is illustrated, but it can also be used for a log. Here, the log and the fungus bed contain water containing minerals. Therefore, it is considered that the log and the fungus bed have a certain degree of conductivity.

  As shown in FIG. 3, the second electrode 121 of the second electrode body 120 is in contact with the lower surface B1b of the fungus bed B1. The dielectric 112 of the first electrode body 110 is in contact with the upper surface B1a of the fungus bed B1. The first electrode 111 of the first electrode body 110 is not in contact with the upper surface B1a of the fungus bed B1.

  As shown in FIG. 3, there is a slight gap between the dielectric 112 of the first electrode body 110 and the upper surface B1a of the fungus bed B1. The gap becomes the plasma emission region P1m. The plasma emission region P1m is a region that emits light by generating plasma.

  As shown in FIG. 3, the plasma emission region P1m is in contact with the fungus bed B1.

  In FIG. 3, the size of the dielectric 112 of the first electrode body 110 is smaller than the size of the upper surface B1a of the fungus bed B1 when viewed from vertically above. Therefore, the plasma light emission region P1m is in contact with a part of the upper surface B1a of the fungus bed B1. The upper surface B1a of the fungus bed B1 includes a region irradiated with plasma and a region not irradiated with plasma. That is, atmospheric pressure plasma is irradiated to a part of the upper surface B1a of the fungus bed B1.

3-2. Second Plasma Generator FIG. 4 is a view (No. 1) showing a state in which the second bed of plasma generator 200 in the present embodiment is used to irradiate plasma on the microbial bed. As shown in FIG. 4, the plasma emission region P2 is in contact with the upper surface B1a of the fungus bed B1. Therefore, ions, radicals, and the like contained in the plasma emission region P2 are irradiated to the bacterial bed B1.

  FIG. 5 is a view (No. 2) showing a state in which plasma is applied to the microbial bed using the second plasma generator 200 in the present embodiment. As shown in FIG. 5, the plasma emission region P2 is not in contact with the upper surface B1a of the fungus bed B1. For this reason, the bacteria bed B1 is irradiated with ions, radicals, and the like contained in the outer region of the plasma emission region P2.

  In this way, the plasma emission region P2 can be brought into contact with the fungus bed B1. Further, the plasma emission region P2 can be prevented from contacting the fungus bed B1. Further, the gas supplied by the gas supply unit 240 can be selected. The gas is preferably Ar. This is because plasma can be suitably generated.

3-3. Difference between the first plasma generation apparatus and the second plasma generation apparatus The first plasma generation apparatus 100 can generate a planar plasma. Therefore, it is possible to efficiently irradiate the raw wood or the fungus bed with plasma. The plasma generated by the first plasma generator 100 is non-equilibrium atmospheric pressure plasma. The plasma gas is the atmosphere around the first plasma generator 100. Therefore, relatively many molecules, ions, and radicals derived from nitrogen atoms and oxygen atoms are generated in the plasma.

  The second plasma generation apparatus 200 can irradiate plasma using a gas supplied from the gas supply unit 240. For example, if Ar gas is used, plasma is easily generated. In this case, it is considered that there are many excited Ar atoms in the plasma emission region P2. In the external region of the plasma emission region P2, it is considered that the surrounding atmosphere is entrained in the plasma, and relatively many molecules, ions, and radicals derived from nitrogen atoms or oxygen atoms originating from the surrounding atmosphere are generated.

3-4. Plasma Irradiation Conditions The time for continuously irradiating the log or fungus bed once is, for example, 30 seconds or more and 5 minutes or less. Preferably, it is 1 minute or more and 2 minutes or less. This is because if the plasma irradiation time per time is long, the surface temperature of the raw wood or fungus bed may increase. The surface temperature of the log or fungus bed is preferably 200 ° C. or less. For this purpose, an interval may be provided as appropriate. When the plasma irradiation time per time is 1 minute, the number of plasma irradiations is preferably 3 to 5 times. The above is an example, and conditions other than the above may be used.

  As described above, when the first plasma generator 100 is used, a part of the upper surface B1a of the fungus bed B1 is irradiated with atmospheric pressure plasma. The irradiation area is preferably as follows. The ratio of the area of the dielectric 112 to the total area of the upper surface B1a of the fungus bed B1 is, for example, 5% or more and 50% or less. Here, the total area of the upper surface B1a of the fungus bed B1 is not the surface area of the upper surface B1a but the area of the upper surface B1a when viewed from vertically above. The area ratio may be a numerical range other than the above. Preferably, it is 5% or more and 35% or less. More preferably, it is 5% or more and 20% or less.

4). Production method of Mannentake The production method of Mannentake of the present embodiment includes an inoculation step of inoculating a raw wood or fungus bed with a strain of Mannentake, a culture step of culturing the raw wood or fungus bed in a cultured log or culture fungus, A plasma irradiation step of irradiating the cultured fungus bed with plasma, and a cultivation step of cultivating the cultured log or the cultured fungus bed to obtain fruit bodies of the garlic mushroom.

4-1. Material Preparation Process The raw wood used for cultivation of mannentake is preferably hardwood or conifer. Examples of broad-leaved trees include kunugi, konara, and beech. Examples of coniferous trees include pine, fir, and tsuga. The above tree can be cut by slicing or the like, placed in a plastic bag or the like, and heat sterilized by a known method. The sterilization method may be appropriately selected.

  The fungus bed used for the cultivation of garlic mushroom is a medium appropriately containing a carbon source, a nitrogen source, an inorganic substance, and other necessary nutrients necessary for the strain to be assimilated. Examples of the fungus bed include those obtained by appropriately mixing sawdust (for example, hardwood such as cucumber, oak, beech, etc., or conifers such as pine, fir, and tsuga), rice bran, bran, corn cobmeal, and water. It is done. And these culture media can be put into the commercially available plastic bag etc. for fungus bed cultivation, and can be heat-sterilized by a well-known method. The sterilization method may be appropriately selected.

4-2. Inoculation process Inoculate the mannambose strain on the log or fungus bed. A known method may be used as the inoculation method.

4-3. Cultivation process A cultivated strain of Mannentake inoculated on the raw wood or fungus bed is allowed to propagate. This culturing step may be performed in a dark place. Of course, it is not limited to dark places. The culture temperature is, for example, 15 ° C. or more and 35 ° C. or less. Preferably, they are 20 degreeC or more and 30 degrees C or less. The culture humidity is, for example, 40% or more and 80% or less. Preferably, it is 55% or more and 65% or less. The culture period in the raw wood is, for example, 40 days or more and 90 days or less. The culture period in the fungus bed is preferably, for example, 30 days or more and 80 days or less. Of course, the culture conditions are not limited to the above.

  In many cases, the culturing process is completed at the stage where the whole wood or mycelium bed is uniformly covered with white mycelia. At this stage, the mycelium from the strain is prevalent in the log or mycelium. Thus, the raw wood in which the mycelium has spread after completion of the culture is called “cultured raw wood”. In addition, the mycelium in which the mycelium is prevalent after completion of the culture is referred to as “cultured mycelia”.

4-4. Plasma irradiation process A plasma irradiation process is implemented with respect to the culture log or culture | cultivation bed which the culture process complete | finished. For example, plasma is irradiated 3 to 5 times. For example, the irradiation time per time is 1 minute.

4-5. Cultivation process Cultivated raw wood or cultured fungus bed that has been irradiated with plasma is grown to the fruit body of Mannentake. The fruit body of the garlic mushroom grows mainly from the upper surface of the cultured log or the cultured fungus bed. This upper surface includes a region irradiated with plasma and a region not irradiated with plasma. The effect of irradiating plasma extends to an unirradiated region that is not irradiated with plasma. Cultivation temperature is 20 degreeC or more and 35 degrees C or less, for example. Preferably, it is 25 degreeC or more and 30 degrees C or less. The cultivation humidity is, for example, 70% or more. Preferably it is 90% or more. By going through the cultivation in this way, mannentake is obtained.

5. Difference from the prior art In the past, a large current was passed through the wood. As a result, it is believed that some action occurs and fungi grow actively.

  In this embodiment, a non-equilibrium atmospheric pressure plasma is irradiated to a cultured log or a cultured fungus bed. Therefore, there is a possibility that charged particles reach the cultured log or the cultured fungus bed. Therefore, a very weak current may flow through the culture log or the culture fungus bed. However, it is not intended to cause a high current to flow through the bedwood as in the prior art.

  Moreover, by irradiating with plasma, ions and radicals derived from nitrogen atoms and oxygen atoms are irradiated to the cultured log or the cultured fungus bed. It is expected that the components of Mannentake will change due to such stimulation by ions and radicals. For example, it is expected that the content of the antioxidant substance in Mannentake increases due to stimulation with radicals having strong oxidizing action.

6). Effects of the present embodiment 6-1. Yield amount The method for producing garlic mushrooms of the present embodiment increases the yield of garlic mushrooms. By irradiating with plasma, it is considered that the strains of Mannentake are activated. A dielectric material insulates the first electrode from the second electrode. The object O1 is mainly put in a plastic bag or the like. Therefore, the log or the fungus bed and the second electrode are insulated. Therefore, it is considered that the current does not flow through the log or the fungus bed, or even if the current flows through the log or the fungus bed, the current is very small.

6-2. Antioxidant Effect The method for producing mannentake of this embodiment enhances the antioxidant effect of mannentake. For example, it is conceivable that the concentration of the antioxidant substance in Mannentake is high.

6-3. Ganoderic acid The method for producing garlic mushrooms of the present embodiment increases ganoderic acid in the garlic mushrooms. Ganoderic acid has an anticancer effect, a blood pressure lowering effect, a cholesterol lowering effect, and the like.

6-4. Plasma In this embodiment, non-equilibrium atmospheric pressure plasma is irradiated to a cultured log or a cultured fungus bed. Thereby, it is considered that a small amount of active oxygen species is irradiated on the mycelia and the like, and the bacteria are activated. Actually, once the mycelium is broken, it is observed that the mycelium spreads in a mesh pattern when the mycelium is regenerated.

7. Modification 7-1. First Electrode of First Plasma Generator The first electrode 111 may have a curved surface on the side facing the second electrode 121. That is, the first electrode 111 may be concave or convex with respect to the second electrode 121. The first electrode 111 may have a bent surface. In addition to the above, the first electrode 111 may have an uneven shape.

  The dielectric 112 of the first electrode body 110 may also have a curved surface or a bent surface corresponding to the shape of the first electrode 111. The first plasma generator may include a flat plate-shaped first electrode 111 and a concavo-convex dielectric 112. As described above, the dielectric 112 may have a shape other than the shape corresponding to the first electrode 111.

7-2. Second electrode of first plasma generating device Instead of disposing the flat plate-like second electrode 121 under the object O1, another second electrode may be used. For example, you may make it insert a rod-shaped 2nd electrode in a log or a fungus bed. In this case, the second electrode is in conduction with the log or the fungus bed. Further, the belt-like second electrode may be wound around the log or the fungus bed together with the plastic bag.

7-3. Plasma Irradiation Conditions The number of plasma irradiations and the continuous plasma irradiation time may of course be changed as appropriate.

7-4. Combination The above modification examples may be freely combined.

8). Summary of the present embodiment In the method for producing mannentake of the present embodiment, plasma is irradiated to a culture log or cultivated fungus bed of mannentake. Therefore, it is possible to increase the yield of garlic mushrooms. In addition, the content of ganoderic acid in Mannentake can be increased.

(Second Embodiment)
A second embodiment will be described. The second embodiment is a method for extracting a component from a bamboo shoot.

1. Mannentake and its extract The fruit body of Mannentake or its extract is used for, for example, pharmaceuticals, quasi-drugs, cosmetics (external preparations for skin), foods and the like. Moreover, the fruit body of Mannentake or its extract also has a function as an antioxidant.

  Mannentake fruit bodies themselves can be chopped or crushed and used for tea or sprinkling.

  The extract of Mannentake can be used for any of pharmaceuticals, quasi drugs, cosmetics, and foods. As the dosage form, for example, lotion, cream, massage cream, emulsion, gel, aerosol, essence, pack, cleaning agent, bath preparation, foundation, powder, lipstick, ointment, poultice, paste, plaster , Powders, pills, tablets, injections, suppositories, emulsions, capsules, granules, solutions (including tinctures, fluid extracts, spirits, suspensions, limonades, etc.), tablets, syrups And beverages.

  In the case of external use, the content of the extract of Mannentake is preferably 0.0001% by weight or more based on the total amount in terms of solid matter. Preferably, it is 0.001 weight% or more and 10 weight% or less. More preferably, it is 0.01 weight% or more and 5 weight% or less. When the amount of the extract of Mannentake is less than 0.0001% by weight, a sufficient effect cannot be expected. When the amount of the extract of Mannentake exceeds 10% by weight, the effect is not so much enhanced.

  In the case of internal use, the intake varies depending on age, weight, symptoms, therapeutic effect, administration method, treatment time and the like. The daily intake per adult is preferably 5 mg or more. Preferably, it is 10 mg or more and 5 g or less. More preferably, it is 100 mg or more and 1 g or less.

2. Extraction method of salmon bamboo The extraction method of salmon bamboo of this embodiment is mainly heating extraction or normal temperature extraction. Alternatively, other known extraction methods may be used.

2-1. Extraction by heating A solvent (extraction solvent) is added to a dried product of Mannentake to obtain a mixture, and components of Mannentake are extracted while heating the mixture to obtain an extract.

2-2. Extraction at room temperature A solvent (extraction solvent) is added to the dried product of Mannen bamboo to make a mixture, and the mixture can be extracted at room temperature to extract the components of Mannen bamboo.

  As an extraction solvent, for example, water, lower alcohol (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol), liquid polyhydric alcohol (1,3-butylene glycol, propylene glycol, glycerin, etc.) ), Ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether, etc.) Can be mentioned. Preference is given to polar solvents such as water, lower alcohols and liquid polyhydric alcohols. Particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.

3. Modification 3-1. Treatment The extract of Mannentake may be used as it is in the extracted solution. Moreover, you may process the extracted solution as needed, such as concentration, dilution, filtration, decolorization, deodorization, and drying. Furthermore, the active ingredient may be concentrated or isolated by performing column purification such as HPLC on ganoderic acid or the like.

3-2. Additives Additives may be added to the extract of Mannentake as long as the effect of the extract is not impaired. Examples of additives include fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, powders, UV absorbers, Examples include a thickener, a dye, an antioxidant, a whitening agent, a chelating agent, a diluent such as lactose and microcrystalline cellulose, a lubricant, and a disintegrant.

3-3. Combination The above modification examples may be freely combined.

  In the examples, “%” indicates wt%. “Parts” refers to parts by weight.

1. Cultivation method 1-1. Raw wood cultivation The kind of raw wood is hardwood (Quercus genus). Logs cut to a size of 5 to 25 cm in diameter and about 13 cm in height were used. Water absorbent paper was laid on the bottom of a polypropylene bag, and 3 kg of cut wood and 120 mL of moisture retaining water were placed in the bag. The bag was lightly closed, and the raw wood in the bag was sterilized by steam and allowed to cool to room temperature in a clean space.

  Next, the log was inoculated with 65 mL of a commercially available reishi inoculum (product name: Mannentake, Akiyama Inoculum Research Institute). After inoculation, the bag mouth was closed. Thereafter, the cells were cultured in a dark place at a temperature of 24 to 30 ° C. and a humidity of 40 to 75%. The whole raw wood was cultured until it was covered with white mycelia to obtain a cultured raw wood. The culture period was 40-50 days.

  The cultured log was irradiated with atmospheric pressure plasma. The plasma irradiation conditions (A-G) will be described later. After the plasma irradiation, the plant was cultivated at a temperature of 25-30 ° C., a humidity of 90-100%, and an illuminance of 3000 lux or less. Mannotake fruit bodies were obtained after cultivation for 50-120 days.

1-2. The type of raw wood used in the fungal bed culture medium is hardwood (Quercus genus). The raw wood was pulverized to a size of 1 to 15 mm with a pulverizer. 0.9 kg of ground wood, 0.1 kg of bran, and 1.5 kg of water were mixed to prepare a culture medium, which was put in a polypropylene bag and used as a fungus bed. The bag was lightly closed, the medium was heat sterilized with steam and allowed to cool to room temperature in a clean space.

  Next, 54 mL of a commercially available reishi inoculum (product name: Mannentake, manufactured by Akiyama Inoculum Research Institute) was inoculated into the medium. After inoculation, the bag mouth was closed. Thereafter, the cells were cultured in a dark place at a temperature of 15 to 30 ° C. and a humidity of 45 to 75%. The whole culture medium was cultured until it was covered with white by the mycelium to obtain a cultured bacterial bed. The culture period was 60 to 120 days.

  The culture cell bed was irradiated with atmospheric pressure plasma. The plasma irradiation conditions (A-F) will be described later. After the plasma irradiation, the plant was cultivated at a temperature of 24-28 ° C., a humidity of 75-85%, and an illuminance of 3000 lux or less. After 30 days of cultivation, Mannentake fruiting bodies were obtained.

2. Plasma Irradiation Conditions Tables 1 to 7 show the atmospheric pressure plasma irradiation conditions for irradiating the cultured log or the cultured fungus bed. In the irradiation condition A-C, the pen-type second plasma generator 200 was used, and in the irradiation condition D-G, the planar first plasma generator 100 was used. In the case of using a flat type plasma generator (first plasma generator 100), a surface obtained by projecting the plasma irradiation region onto the upper surface of the cultured bacterial bed, which occupies the entire area of the upper surface of the cultured log or the cultured bacterial bed. The area ratio was about 7% to 11%. In addition, the cultured log and the cultured fungus bed are cultured in a state of being put in a polypropylene bag. Therefore, the plasma was irradiated in a state where the cultured log and the cultured fungus bed were put in a bag. Therefore, for irradiation conditions E and G, plasma was irradiated in a state where polypropylene was present between the second electrode and the cultured log or cultured fungus bed. Even in this case, a plasma emission region was generated between the first electrode and the cultured log or the cultured fungus bed.

[Table 1]
Irradiation condition A
Plasma device Pen type (second plasma generator)
Gas type Ar
Gas flow rate 3L / min
Applied voltage 5.2 kV
Number of times of irradiation 3 times (interval 1 minute)
Irradiation time 1 minute (total irradiation time 3 minutes)
Irradiation distance 20mm (from irradiation port 201a to fungus bed upper surface B1a)
Luminescent area Non-contact with target

[Table 2]
Irradiation condition B
Plasma device Pen type (second plasma generator)
Gas type Ar
Gas flow rate 3L / min
Applied voltage 6.5kV
Number of times of irradiation 3 times (interval 1 minute)
Irradiation time 2 minutes (total irradiation time 6 minutes)
Irradiation distance 10mm (from irradiation port 201a to fungus bed upper surface B1a)
Light emitting area Contact with target

[Table 3]
Irradiation condition C
Plasma device Pen type (second plasma generator)
Gas type Ar
Gas flow rate 3L / min
Applied voltage 5.2 kV
Number of irradiations 3 times (1 minute interval)
Irradiation time 1 minute (total irradiation time 3 minutes)
Irradiation distance 10mm (from irradiation port 201a to fungus bed upper surface B1a)
Light emitting area Contact with target

[Table 4]
Irradiation condition D
Plasma device Planar type (first plasma generator)
1st electrode 50 × 50mm
Position of the first electrode Near the edge of the upper surface of the cultured log or the cultured fungus bed Second electrode φ1 110 mm (insert the tungsten rod into the object)
Position of the second electrode Among the side surfaces with a height of 10 mm from the lower surface (B1b)
Position closest to the first electrode body (100mm insertion)
Applied voltage 5.2 kV
Number of times of irradiation 3 times (interval 1 minute)
Irradiation time 1 minute (total irradiation time 3 minutes)
Irradiation distance 0mm
Light emitting area Contact with target

[Table 5]
Irradiation condition E
Plasma device Planar type (first plasma generator)
1st electrode 50 × 50mm
Position of the first electrode Near the edge of the upper surface of the cultured log or the cultured bacterial bed Second electrode 250 × 200 mm (wider than the bottom area of the object)
Position of the second electrode Arranged just below the cultured log or cultured fungus bed Applied voltage 5.2 kV
Number of times of irradiation 3 times (interval 1 minute)
Irradiation time 1 minute (total irradiation time 3 minutes)
Irradiation distance 0mm
Light emitting area Contact with target

[Table 6]
Irradiation condition F
Plasma device Planar type (first plasma generator)
1st electrode 50 × 50mm
Position of the first electrode Center of the upper surface of the cultured log or cultured fungus bed Second electrode φ1 110 mm (insert a tungsten rod into the object)
Position of the second electrode Among the side surfaces with a height of 10 mm from the lower surface (B1b)
Position closest to the first electrode body (100mm insertion)
Applied voltage 5.2 kV
Number of times of irradiation 3 times (interval 1 minute)
Irradiation time 1 minute (total irradiation time 3 minutes)
Irradiation distance 0mm
Light emitting area Contact with target

[Table 7]
Irradiation condition G
Plasma device Planar type (first plasma generator)
1st electrode 50 × 50mm
Position of the first electrode Near the edge of the upper surface of the cultured log or the cultured bacterial bed Second electrode 50 × 750mm
Position of the second electrode Wrapped below the side of the culture log or culture fungus Applied voltage 5.2 kV
Number of times of irradiation 3 times (interval 1 minute)
Irradiation time 1 minute (total irradiation time 3 minutes)
Irradiation distance 0mm
Light emitting area Contact with target

3. Table 8 shows the yield of Mannen bamboo (adjusted to a water content of 10% by weight) obtained by log cultivation and fungus bed cultivation. In the cultivation examples 1A to 1G, the harvesting amount when the harvesting amount of the raw wood cultivation not irradiated with plasma is set to 100 is shown. In cultivation example 2A-2F, the harvest amount when the harvest amount of the fungus bed cultivation not irradiated with plasma is set to 100 is shown.

[Table 8]
Sample Cultivation method Irradiation condition Harvest amount Cultivation example 1A Log cultivation Irradiation condition A 109
Cultivation example 1B Log cultivation Irradiation condition B 106
Cultivation example 1C Log cultivation Irradiation condition C 97
Cultivation example 1D Log cultivation Irradiation condition D 122
Cultivation example 1E Log cultivation Irradiation condition E154
Cultivation example 1F Log cultivation Irradiation condition F 130
Cultivation example 1G Log cultivation Irradiation condition G130
Cultivation example 2A Fungus bed cultivation Irradiation condition A 128
Cultivation example 2B Fungus bed cultivation Irradiation condition B 121
Cultivation example 2C Fungus bed cultivation Irradiation condition C 106
Cultivation example 2D Fungus bed cultivation Irradiation condition D 106
Cultivation example 2E Fungus bed cultivation Irradiation condition E 112
Cultivation example 2F Fungus bed cultivation Irradiation condition F 101

  As shown in Table 8, in the cultivation examples 1D-1G and the cultivation examples 2A-2B, the yield increased by 20% or more compared to the case where the conventional plasma was not used. For log cultivation, it is preferable to use the flat first plasma generator 100. For fungus bed cultivation, it is preferable to use the pen-type second plasma generator 200.

  When the pen-type second plasma generating apparatus 200 is used, it is preferable to irradiate the cultured log or the cultured fungus bed with atmospheric pressure plasma in a state where the light emitting region P2 is not in contact with the cultured log or the cultured fungus bed (cultivation). Examples 1A-1C, 2A-2C).

  When the flat-type first plasma generator 100 is used, it is preferable to lay a second electrode having a flat shape under a culture log or a culture fungus bed (cultivation examples 1E and 2E). In particular, the increase in yield was significant in the cultivation of raw wood (Cultivation Example 1E).

4). Extract of Mannentake Hereinafter, a method for extracting components from Mannentake will be described.

4-1. Extraction method a (hot water extract)
Purified water (200 mL) was added to 10 g of dried garlic mushroom bamboo cultivated with raw wood or fungus bed, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and freeze-dried to obtain a hot water extract. .

4-2. Extraction method b (50% ethanol extract)
200 g of 50% ethanol was added to 10 g of dried garlic mushrooms grown on raw wood or fungus bed, extracted for 7 days at room temperature, filtered, and the filtrate was concentrated to dryness to obtain a 50% ethanol extract.

4-3. Extraction method c (ethanol extract)
Ethanol (200 mL) was added to 10 g of dried garlic mushroom bamboo or fungus bed cultivated, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain an ethanol extract.

  Table 9 shows the weight of the extract of Mannentake in each production example. In Table 9, “hot water” indicates that the hot water extraction method was used. “Water + ethanol” indicates that the extraction method using 50% ethanol was used. “Ethanol” indicates that an extraction method using ethanol was used.

  The cultivation examples 1A-2F and the extraction methods a-c were combined and summarized as Production Example 1Aa-2Fc. For example, “Production Example 1Aa” means that it has been cultivated by “Cultivation Example 1A” and extracted by “Extraction Method a”.

5. Antioxidant action of extract of Mannentake 5-1 Active oxygen species α, α-diphenyl-β-picrylhydrazyl (hereinafter referred to as “DPPH”), which is a kind of free radical, was used as the active oxygen species.

5-2. Experimental Method An extract corresponding to Production Example 1Aa-2Fc described above was added to 0.4 mL of 0.1 M acetate buffer (pH 5.5) to which a final concentration of 100 μg / mL was added, and 0.4 mL of absolute ethanol and 0. A reaction solution was prepared by adding 0.2 mL of 5 mM DPPH absolute ethanol solution. In addition, a sample was added to 0.4 mL of absolute ethanol to the oil-soluble extract of Production Example 1Aa-2Fc to prepare a reaction solution. Then, it was made to react for 30 minutes at 37 degreeC, and the light absorbency (J1) of wavelength 517nm was measured by using water as a control. Moreover, the light absorbency (J2) was measured using purified water instead of the sample as a blank. The DPPH radical scavenging rate was calculated from the following formula.
DPPH radical scavenging rate (%) = (1−J1 / J2) × 100

5-3. DPPH radical scavenging rate Table 11 shows the DPPH radical scavenging rate for each production example. As shown in Table 11, all the extracts of Mannentake have a DPPH radical scavenging action. The extract of banyan mushroom, which was obtained by irradiating a cultured log or a cultivated fungus bed with plasma, exhibited a higher antioxidant effect than the unirradiated mannentake extract. In particular, Production Examples 1Aa and 2Aa, in which the pen-shaped second plasma generator 200 was used to irradiate the plasma without bringing the light emitting region into contact with the cultured log or the cultured fungus bed, showed a strong antioxidant effect. In addition, Production Examples 2Da and 2Ea using the flat-type first plasma generator 100 also showed a strong antioxidant effect.

[Table 11]
DPPH radical scavenging rate Production Example 1Aa (Rawwood / Irradiation condition A / Hot water) 46
Production Example 1Ca (Rawwood, Irradiation Condition C, Hot Water) 41
Comparative Production Example 1a (Raw Wood / Unirradiated / Hot Water) 37
Production Example 2Aa (bacteria bed / irradiation condition A / hot water) 45
Production Example 2Ba (bacteria bed / irradiation condition B / hot water) 35
Production Example 2Da (Bacteria Bed / Irradiation Condition D / Hot Water) 41
Production Example 2Ea (bacteria bed / irradiation condition E / hot water) 44
Production Example 2 Fa (bacteria bed / irradiation condition F / hot water) 35
Comparative Production Example 2a (bacteria bed / unirradiated / hot water) 33

6). Content of ganoderic acid Ganoderic acid has effects such as anticancer action, blood pressure lowering action, and cholesterol lowering action.

6-1. Measuring method About the extract of manufacture example 1Aa-2Fc, content of ganoderic acid was measured using HPLC. Table 12 shows the HPLC conditions. The mobile phase K2 was linearly changed from 10% to 94% in 60 minutes. The flow rate was adjusted so that the retention time of pyrene was about 52 minutes. For example, 1.0 mL / min.

[Table 12]
Column: Develosil ODS-HG-5 (manufactured by Nomura Chemical)
Column size: inner diameter 4.6mmφ x length 250mm
Column temperature: 40 ° C
Sample concentration: 1.0 mg / mL
Injection volume: 10 μL
Detection: PDA 254nm
Eluent (gradient):
Mobile phase K1 10 mM sodium phosphate buffer (pH 6.9)
Mobile phase K2 Acetonitrile Internal standard solution: Ethylene solution of pyrene (1 mg / mL)

6-2. FIG. 6 is a graph showing an HPLC chromatogram of Ganoderma extract. As shown in FIG. 6, a large number of peaks derived from ganoderic acids having a maximum absorption around 254 nm were confirmed within a retention time of 15 to 25 minutes.

  Table 13 shows the peak area values derived from ganoderic acids. Here, the area value in the case of non-irradiation was set to 1. That is, for Production Example 1Ac-1Fc, Comparative Production Example 1c was used as a reference. Production Example 2Ac-2Fc was based on Comparative Production Example 2c. As is clear from Table 13, the content of ganoderic acid in the garlic mushrooms is increased by irradiating the culture log or the culture fungus bed with atmospheric pressure plasma. The manufacture example which irradiated the atmospheric pressure plasma using the pen-type 2nd plasma generator 200 contains comparatively much ganoderic acid (manufacture example 1Ac, 2Ac).

  In addition, Production Example 2Ec contains a large amount of ganoderic acid. The content of ganoderic acid in Production Example 2Ec is about 40% higher than that of the sample not irradiated with plasma. Further, as described above, Production Example 2Ec shows a high antioxidant effect.

[Table 13]
Ganoderic acid content Production Example 1Ac (Raw wood / Irradiation condition A / Ethanol) 1.09
Production Example 1 Ec (Rawwood, Irradiation Condition E, Ethanol) 1.01
Production Example 1 Gc (Rawwood / Irradiation Condition G / Ethanol) 1.06
Comparative production example 1c (raw wood, unirradiated, ethanol) 1.00
Production Example 2Ac (bacteria bed / irradiation conditions A / ethanol) 1.07
Production Example 2Dc (bacteria bed / irradiation conditions D / ethanol) 1.05
Production Example 2Ec (bacteria bed / irradiation conditions E / ethanol) 1.39
Production Example 2 Fc (bacteria bed / irradiation conditions F / ethanol) 1.05
Comparative production example 2c (bacteria bed, unirradiated, ethanol) 1.00

7. Formulation Example A formulation example in which an extract of Mannentake was prepared according to Production Example 1Aa to Production Example 2Fc is shown. Here, the mixed extract is a mixture of equal amounts of the extract in each production example.

7-1. Formulation Example 1 Skin Lotion Components 1 to 6 and 11 and Tables 7 to 10 in Table 14 are uniformly dissolved, and both are mixed and filtered. Thereby, the skin lotion as a product is obtained.

[Table 14]
Lotion Prescription Content (parts)
1. Production Example 1 Aa, 1 Da mixed extract 1.0
2.1,3-Butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5. Citric acid 0.01
6). Sodium citrate 0.1
7. Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Perfume proper amount11. Make the total volume 100 with purified water

7-2. Formulation Example 2 Cream Components 2 to 9 in Table 15 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 11-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase and emulsified. Then, component 10 is added at 45 ° C. while cooling the emulsified solution while stirring, and further cooled to 30 ° C. Thereby, the cream as a product is obtained.

[Table 15]
Cream Formulation Content (parts)
1. Production Example 1Ab, 1Bb, 1Cb, 1Db, 1Eb mixed extract 0.5
2. Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5. Beeswax 2.0
6). Octyldodecyl myristate 3.5
7. Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11. Methyl paraoxybenzoate 0.25
12.1,3-Butylene glycol 8.5
13. Make the total volume 100 with purified water

7-3. Formulation Example 3 Cream The extract of Production Example 1Ab in Table 15 is replaced with the extract of Production Example 2Ab.

7-4. Formulation Example 4 Cream The extract of Production Example 1Ab in Table 15 is replaced with the extract of Production Example 2Bb.

7-5. Formulation Example 5 Cream The extract of Production Example 1Ab in Table 15 is replaced with the extract of Production Example 2Cb.

7-6. Formulation Example 6 Emulsion Ingredients 2 to 8 in Table 16 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase and emulsified. Thereafter, while cooling the emulsified solution while stirring, component 9 is added at 45 ° C., and further cooled to 30 ° C. Thereby, the emulsion as a product is obtained.

[Table 16]
Emulsion Formulation Content (parts)
1. Production Example 1Ba, 1Ca, 1Ea, 1Fa, 1Ga mixed extract 1.0
2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7. Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. Make the total volume 100 with purified water

7-7. Formulation example 7 gel agent The components 1-5 of Table 17 and the components 6-11 are each melt | dissolved uniformly, and both are mixed. Thereby, the gel agent as a product is obtained.

[Table 17]
Gel formulation Prescription Content (parts)
1. Production Example 1Ac, 1Dc, 1Ec, 2Ac, 2Ec mixed extract 0.01
2. Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4). Polyoxyethylene hydrogenated castor oil (60 EO) 0.1
5. Perfume appropriate amount 6.1,3-butylene glycol 5.0
7. Glycerin 5.0
8). Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. Make the total volume 100 with purified water

7-8. Formulation example 8 pack The components 1-11 of Table 18 are melt | dissolved uniformly. Thereby, a pack as a product is obtained.

[Table 18]
Pack Prescription Content (parts)
1. Production Example 2 Aa, 2Fa mixed extract 0.1
2. Production Example 2 Da, 2Ea mixed extract 0.1
3. Polyvinyl alcohol 12.0
4). Ethanol 5.0
5.1,3-butylene glycol 8.0
6). Methyl paraoxybenzoate 0.2
7. Polyoxyethylene hydrogenated castor oil (20 EO) 0.5
8). Citric acid 0.1
9. Sodium citrate 0.3
10. Perfume proper amount11. Make the total volume 100 with purified water

7-9. Formulation example 9 foundation The components 2-8 of Table 19 are heat-dissolved, and it maintains at 80 degreeC and makes it an oil phase. Swell component 9 well in component 19, then add components 1 and 10-13 and mix uniformly. To this, components 14 to 17 pulverized and mixed with a pulverizer are added, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to this oil phase while stirring and emulsified. Then it is cooled, component 18 is added at 45 ° C. and cooled to 30 ° C. with stirring. Thereby, the foundation as a product is obtained.

[Table 19]
Foundation Prescription Content (parts)
1. Production Example 1 Fb, 1Gb, 2Db, 2Eb, 2Fb mixed extract 1.0
2. Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate (20E.O.) 1.0
4). Polyoxyethylene cetyl ether (20E.O.) 2.0
5. Cetanol 1.0
6). Liquid lanolin 2.0
7. Liquid paraffin 3.0
8). Isopropyl myristate 6.5
9. Sodium carboxymethylcellulose 0.1
10. Bentonite 0.5
11. Propylene glycol 4.0
12 Triethanolamine 1.1
13. Methyl paraoxybenzoate 0.2
14 Titanium dioxide 8.0
15. Talc 4.0
16. Bengala 1.0
17. Yellow iron oxide 2.0
18. Perfume appropriate amount 19. Make the total volume 100 with purified water

7-10. Formulation Example 10 Bath Agent Components 1 to 6 in Table 20 are mixed uniformly. Thereby, the bath agent as a product is obtained.

[Table 20]
Bath preparation Prescription Content (parts)
1. Production Example 1 Extract of Ea 5.0
2. Extract of Production Example 2Aa 1.0
3. Sodium bicarbonate 50.0
4). Yellow No. 202 (1) Appropriate amount 5. Perfume appropriate amount 6. Bring the total amount to 100 with sodium sulfate

7-11. Formulation Example 11 Ointment Components 2 to 5 in Table 21 are dissolved by heating and mixed, and the mixture is kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 6 to 8 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled to 30 ° C. while stirring. Thereby, an ointment as a product is obtained.

[Table 21]
Ointment Formulation Content (parts)
1. Production Example 1Ab, 1Eb, 2Ab, 2Eb mixed extract 0.5
2. Polyoxyethylene cetyl ether (30E.O.) 2.0
3. Glycerol monostearate 10.0
4). Liquid paraffin 5.0
5. Cetanol 6.0
6). Methyl paraoxybenzoate 0.1
7. Propylene glycol 10.0
8). Make the total volume 100 with purified water

7-12. Formulation example 12 powder The components 1-3 of Table 22 are mixed. Thereby, the powder as a product is obtained.

[Table 22]
Powder Formulation Content (parts)
1. Production Example 1Aa, 1Ea, 2Aa, 2Ea mixed extract 20.0
2. Dried corn starch 30.0
3. Microcrystalline cellulose 50.0

7-13. Formulation Example 13 Tablets Components 1 to 4 in Table 23 are mixed, and then an aqueous solution of component 5 is added as a binder and granulated. Ingredient 6 is added to the formed granules and compressed. One tablet is 0.52 g. Thereby, the tablet as a product is obtained.

[Table 23]
Tablet Formulation Content (parts)
1. Production Example 1 Aa, 1Ea, 2Aa, 2Ea mixed extract 3.0
2. Dried cornstarch 27.0
3. Carboxymethylcellulose calcium 20.0
4). Microcrystalline cellulose 40.0
5. Polyvinylpyrrolidone 7.0
6). Talc 3.0

7-14. Formulation Example 14 Tablet Confectionery Components 1 to 4 and 7 in Table 24 are mixed and granulated. Ingredients 5 and 6 are added to the formed granules and compressed. One tablet is 1.0 g. Thereby, the tablet confection as a product is obtained.

[Table 24]
Tablets Prescription Content (parts)
1. Production Example 1 Aa, 1Ea, 2Aa, 2Ea mixed extract 0.5
2. Dried corn starch 50.0
3. Erythritol 40.0
4). Citric acid 5.0
5. Sucrose fatty acid ester 3.0
6). Perfume appropriate amount 7. Make the total volume 100 with purified water

7-15. Formulation Example 15 Beverage Components 1 to 5 in Table 25 are mixed. Thereby, the drink as a product is obtained.

[Table 25]
Beverage Prescription Content (parts)
1. Production Example 1 Aa, 1Ea, 2Aa, 2Ea mixed extract 2.0
2. Fructose dextrose liquid sugar 12.5
3. Citric acid 0.1
4). Fragrance 0.05
5. Make the total volume 100 with purified water

7-16. Formulation example 16 powdered drink The components 1-8 of Table 26 are mixed. Thereby, the powdered drink as a product is obtained.

[Table 26]
Formulation Example 16 Powdered drink Formulation Content (parts)
1. Production Example 1Aa, 1Ea, 2Aa, 2Ea mixed extract 10.0
2. Powdered sugar 65.0
3. Powdered peach juice 15.0
4). L-ascorbic acid 8.0
5. Crystalline citric acid 1.2
6). Sodium citrate 0.75
7. Aspartame 0.02
8). Powdered peach flavor 0.03

A. Supplementary Note In the method for producing mannentake in the first aspect, a plasma generator having a first electrode body including a first conductor is used. Plasma is irradiated to the culture log or the culture fungus in a state where the first conductor of the plasma generator is not in direct contact with the culture log or the culture fungus bed.

  In the method for producing mannentake in the second aspect, the first electrode body has a dielectric. In a state where the dielectric is located between the first conductor and the cultured log or the cultured fungus bed, the plasma is irradiated to the cultured log or the cultured fungus bed.

  In the method for producing mannentake in the third aspect, the plasma generator includes a second electrode body having a second conductor, a voltage applying unit that applies a voltage between the first conductor and the second conductor, Have

  In the method for producing garlic mushrooms according to the fourth aspect, the voltage application unit is configured such that the first conductor and the second conductor are in a state where the cultured log or the cultured fungus bed is disposed on the second conductor of the second electrode body. A voltage is applied between the body and the body.

  In the method for producing mannentake in the fifth aspect, the plasma generator generates a light emitting region. Plasma is irradiated to the cultured log or the cultured fungus bed in a state where the luminescent region is not in contact with the cultured log or the cultured fungus bed.

  The method for producing an antioxidant in the sixth aspect includes the steps of the above-mentioned method for producing garlic mushrooms and an extraction step for extracting components from the garlic mushrooms.

  According to a seventh aspect of the present invention, there is provided a method for producing a pharmaceutical product comprising the steps of the above-described method for producing garlic mushroom and an extraction step for extracting components from the garlic mushroom.

  The food production method according to the eighth aspect includes the steps of the above-mentioned Mannentake production method and the extraction step of extracting components from the Mannentake.

  The method for producing an external preparation for skin according to the ninth aspect includes the steps of the above-mentioned method for producing garlic mushroom and the extraction step for extracting components from the garlic mushroom.

DESCRIPTION OF SYMBOLS 100 ... 1st plasma generator 200 ... 2nd plasma generator 110, 210 ... 1st electrode body 111, 211 ... 1st electrode 112, 212 ... Dielectric 120, 220 ... 2nd electrode body 121, 221 ... 1st Two electrodes P1, P2, P1m ... Plasma emission region B1 ... Bacteria bed B1a ... Upper surface B1b ... Lower surface

Claims (9)

Using a plasma generator having a first electrode body with a first conductor,
A method for producing garlic mushroom, wherein the plasma is irradiated to the culture log or the culture fungus in a state where the first conductor of the plasma generator is not in direct contact with the culture log or the culture fungus bed. In the manufacturing method of the mushroom of Claim 1,
The first electrode body includes:
Having a dielectric,
Production of garlic mushroom characterized by irradiating the culture log or the culture fungus with plasma in a state where the dielectric is located between the first conductor and the culture log or the culture fungus bed. Method. In the manufacturing method of the mannambose according to claim 1 or claim 2,
The plasma generator comprises:
A second electrode body having a second conductor;
A voltage applying unit that applies a voltage between the first conductor and the second conductor;
A method for producing garlic mushroom, characterized by comprising: In the manufacturing method of the mushrooms of Claim 3,
The voltage application unit includes:
Applying a voltage between the first conductor and the second conductor in a state where the culture log or the culture fungus bed is disposed on the second conductor of the second electrode body. A method for producing garlic mushrooms. In the manufacturing method of the mushroom of Claim 1,
The plasma generator comprises:
Create a luminous area,
A method for producing garlic mushroom, characterized in that plasma is irradiated to the cultured log or the cultured fungus bed in a state where the luminescent region is not brought into contact with the cultured log or the cultured fungus bed. The process of the production method of the garlic mushroom according to any one of claims 1 to 5,
An extraction step of extracting components from the mannambo,
A method for producing an antioxidant, comprising: The process of the production method of the garlic mushroom according to any one of claims 1 to 5,
An extraction step of extracting components from the mannambo,
A method for producing a pharmaceutical product characterized by comprising: The process of the production method of the garlic mushroom according to any one of claims 1 to 5,
An extraction step of extracting components from the mannambo,
A method for producing a food, characterized by comprising: The process of the production method of the garlic mushroom according to any one of claims 1 to 5,
An extraction step of extracting components from the mannambo,
A method for producing an external preparation for skin, characterized by comprising: