JP2018538385A - 流体装置のための充填剤流体 - Google Patents
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Abstract
Description
マイクロ流体デバイスは、通常、サブミリリットルの範囲の小さな流体容量を扱う小型の流体デバイスである。マイクロ流体デバイスは、マイクロメカニカル構造(マイクロチャネル、マイクロトラック、マイクロパス、マイクロバルブおよびその他)を有し、および種々の流体移動機構、例えば、機械部品(例えば、マイクロポンプ)流体圧空気デバイス/方法および電気ベースの効果(電気泳動、誘電泳動、電気浸透、エレクトロウェッティング、オプトエレクトロウェッティング、およびこれらの効果の変形、ならびに他の効果)などのようなものを採用する。
ここに開示するいくつかの実施形態は、マイクロ流体装置のための充填剤流体を提供し、それには、シリコーン油およびシリコーン油において可溶化されるシロキサンブロックコポリマーが含まれ、そこでは、シロキサンブロックコポリマーは水性液体と実質不混和性である。いくつかの実施形態では、シロキサンブロックコポリマーの容量分率の約0.1%未満が充填剤流体において水性液体と混和性である。いくつかの実施形態では、シロキサンブロックコポリマーはシロキサン主鎖および官能化側鎖を含む。いくつかの実施形態では、官能化側鎖は親水性頭部基を含む。いくつかの実施形態では、シロキサンブロックコポリマーは、式I:
前述の概念および以下により一層詳細に論じられる追加の概念(そのような概念が相互に矛盾しないことを条件とする)のすべての組合せが、ここに開示される創意に富む主題の一部であると考えられることを認めるべきである。特に、この開示の最後に現れる請求される主題のすべての組合せは、ここに開示される創意に富む主題の一部であると考えられる。
本開示は、マイクロ流体装置において液滴操作を改善するための組成物および方法を記載する。バイオメディカルの適用では、いくつかのマイクロ流体装置は、濃縮、ろ過、洗浄、分注、混合、輸送、サンプル分割、サンプル溶解、その他のサンプル取扱い機能を含め、サンプル処理を行うように設計される。
定義
充填剤流体
シロキサンベースのブロックコポリマー
によって代表される構造を含み、式中、n≧0、m≧0、およびRには、制限されないが、ポリアクリルアミド、ポリサッカライド(多糖類とも言う)、ポリグリコール、カルボキシラート(カルボン酸塩とも言う)/カルボン酸、スルホナート(スルホン酸塩とも言う)/サルファート(硫酸塩とも言う)、エチレングリコール/PEG、アミン/アンモニウム、カーボハイドラート(炭水化物とも言う)、カルボナート(炭酸塩とも言う)、シリカート(ケイ酸塩とも言う)、等が含まれ得る。
であり、式中、n≧10である。
流体装置
液滴操作を行う方法
キット
において代表され、式中、n≧0、m≧0、およびRは、ポリアクリルアミド、ポリサッカライド、ポリグリコール、カルボキシラート、カルボン酸、スルホナート、サルファート、エチレングリコール、アミン、アンモニウム、カーボハイドラート、カルボナート、およびシリカートからなる群より選ばれる。いくつかの実施形態では、シロキサンブロックコポリマーは、CMS-222、CMS-221、FMS 736、FMS-141、APT-263およびMCR-C12からなる群より選ばれる。いくつかの実施形態において、充填剤流体においてシロキサンブロックコポリマーの濃度は、約0.02%w/wないし約0.1%w/wである。いくつかの実施形態では、充填剤流体においてシロキサンブロックコポリマーの濃度は、約0.05%w/wである。いくつかの実施形態において、充填剤流体はポリジメチルシロキサン(PDMS)を含む。
充填剤流体を負荷する方法
例1
例2
例3
例4
例5
Claims (22)
- 流体装置のための充填剤流体であって、次の:
シリコーン油;および
シリコーン油において可溶化されるシロキサンブロックコポリマー
を含み、シロキサンブロックコポリマーは水性液体と実質不混和性である、充填剤流体。 - 充填剤流体においてシロキサンブロックコポリマーの約0.1%未満の容量分率は水性液体と混和性である、請求項1の充填剤流体。
- シロキサンブロックコポリマーはシロキサン主鎖および官能化側鎖を含む、請求項1の充填剤流体。
- 官能化側鎖には、親水性頭部基が含まれる、請求項3の充填剤流体。
- シロキサンブロックコポリマーの親水性頭部基は、ポリアクリルアミド、ポリサッカライド、ポリグリコール、カルボキシラート、カルボン酸、スルホナート、サルファート、エチレングリコール アミン、アンモニウム、カーボハイドラート、カルボナート、およびシリカートからなる群より選ばれる、請求項4の充填剤流体。
- シロキサンブロックコポリマーは、式I:
において代表され、
式中、n≧0、m≧0、およびRは、ポリアクリルアミド、ポリサッカライド、ポリグリコール、カルボキシラート、カルボン酸、スルホナート、サルファート、エチレングリコール、アミン、アンモニウム、カーボハイドラート、カルボナート、およびシリカートからなる群より選ばれる、請求項5の充填剤流体。 - シロキサンブロックコポリマーの濃度は充填剤流体において約0.02%w/wないし約0.1%w/wである、請求項1の充填剤流体。
- シロキサンブロックコポリマーの濃度は充填剤流体において約0.05%w/wである、請求項1の充填剤流体。
- シリコーン油には、ポリジメチルシロキサン(PDMS)が含まれる、請求項1の充填剤流体。
- シロキサンブロックコポリマーは(ヒドロキシプロピレンオキシプロピル)メチルシロキサン-ジメチルシロキサンコポリマーである、請求項9の充填剤流体。
- 充填剤流体および水性液体の液滴の表面張力は約3および約12ダイン/cmの間である、請求項1の充填剤流体。
- 次の:
流体工学装置;および
充填剤流体が含まれる容器を含み、そこでは、充填剤流体には、シロキサンブロックコポリマーおよびシリコーン油が含まれる、キット。 - シロキサンブロックコポリマーと実質不混和性である水性緩衝剤が含まれる容器をさらに含む、請求項12のキット。
- 充填剤流体においてシリコンブロックコポリマーの容量分率の約0.1%未満は水性緩衝剤と混和性である、請求項13のキット。
- シリコーン油には、ポリジメチルシロキサン(PDMS)が含まれる、請求項0のキット。
- シロキサンブロックコポリマーは、(ヒドロキシプロピレンオキシプロピル)メチルシロキサン-ジメチルシロキサンコポリマーである、請求項15のキット。
- 流体工学装置は、エレクトロウェッティング、オプトエレクトロウェッティング、静電、電気泳動、誘電泳動、または電気浸透装置である、請求項0のキット。
- 流体装置において液滴操作を行うにあたり、
流体装置内の充填剤流体を通して複数の水性液滴を移動させることを含み、そこで、充填剤流体には、シリコーン油において可溶化されるシロキサンブロックコポリマーが含まれ、および複数の水性液滴は充填剤流体と実質不混和性である、方法。 - 複数の水性液滴を移動させることには、複数の水性液滴を移動させるために、エレクトロウェッティング、オプトエレクトロウェッティング、静電、電気泳動、誘電泳動、電気浸透、またはそれらの組合せの使用が含まれる、請求項18の方法。
- 複数の水性液滴および充填剤流体の間の表面張力は、約3および約12ダイン/cmの間である、請求項18の方法。
- 複数の液滴を移動させることには、ポリメラーゼ連鎖反応を実行することが含まれる、請求項18の方法。
- 複数の液滴を移動させることには、ポリヌクレオチド配列決定反応のためにサンプルを用意することが含まれる、請求項18の方法。
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US201562245147P | 2015-10-22 | 2015-10-22 | |
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PCT/US2016/057941 WO2017070363A1 (en) | 2015-10-22 | 2016-10-20 | Filler fluid for fluidic devices |
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WO2023117863A1 (en) | 2021-12-20 | 2023-06-29 | Stilla Technologies | Surfactant for analyzing biological material |
WO2023117864A1 (en) | 2021-12-20 | 2023-06-29 | Stilla Technologies | Micro-droplet generating apparatus |
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- 2016-10-20 CN CN201680068197.XA patent/CN108290157B/zh active Active
- 2016-10-20 EP EP16858226.0A patent/EP3365108A4/en active Pending
- 2016-10-20 WO PCT/US2016/057941 patent/WO2017070363A1/en active Application Filing
- 2016-10-20 CA CA3002983A patent/CA3002983A1/en active Pending
- 2016-10-20 AU AU2016341948A patent/AU2016341948A1/en not_active Abandoned
- 2016-10-20 JP JP2018520514A patent/JP6936222B2/ja active Active
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- 2016-10-20 RU RU2018114925A patent/RU2719991C2/ru active
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- 2018-10-30 HK HK18113839.8A patent/HK1254546A1/zh unknown
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HK1254546A1 (zh) | 2019-07-19 |
EP3365108A4 (en) | 2018-10-17 |
EP3365108A1 (en) | 2018-08-29 |
CA3002983A1 (en) | 2017-04-27 |
CN108290157B (zh) | 2021-08-17 |
RU2018114925A3 (ja) | 2020-02-20 |
JP6936222B2 (ja) | 2021-09-15 |
RU2018114925A (ru) | 2019-11-22 |
WO2017070363A1 (en) | 2017-04-27 |
AU2016341948A1 (en) | 2018-05-10 |
SG11201803293SA (en) | 2018-05-30 |
US20190217300A1 (en) | 2019-07-18 |
CN108290157A (zh) | 2018-07-17 |
US20200179935A1 (en) | 2020-06-11 |
RU2719991C2 (ru) | 2020-04-23 |
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