JP2018531013A5 - - Google Patents
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- Publication number
- JP2018531013A5 JP2018531013A5 JP2018518522A JP2018518522A JP2018531013A5 JP 2018531013 A5 JP2018531013 A5 JP 2018531013A5 JP 2018518522 A JP2018518522 A JP 2018518522A JP 2018518522 A JP2018518522 A JP 2018518522A JP 2018531013 A5 JP2018531013 A5 JP 2018531013A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- engineered nucleic
- acid construct
- acid encoding
- engineered
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000039446 nucleic acids Human genes 0.000 claims 29
- 108020004707 nucleic acids Proteins 0.000 claims 29
- 150000007523 nucleic acids Chemical class 0.000 claims 29
- 101710163270 Nuclease Proteins 0.000 claims 9
- 230000004568 DNA-binding Effects 0.000 claims 7
- 108091033409 CRISPR Proteins 0.000 claims 4
- 102000015694 estrogen receptors Human genes 0.000 claims 4
- 108010038795 estrogen receptors Proteins 0.000 claims 4
- 238000011144 upstream manufacturing Methods 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108020005004 Guide RNA Proteins 0.000 claims 3
- 230000001939 inductive effect Effects 0.000 claims 3
- 238000011830 transgenic mouse model Methods 0.000 claims 3
- 241000699666 Mus <mouse, genus> Species 0.000 claims 2
- 241000699660 Mus musculus Species 0.000 claims 2
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims 2
- NKANXQFJJICGDU-QPLCGJKRSA-N Tamoxifen Chemical compound C=1C=CC=CC=1C(/CC)=C(C=1C=CC(OCCN(C)C)=CC=1)/C1=CC=CC=C1 NKANXQFJJICGDU-QPLCGJKRSA-N 0.000 claims 2
- 102000034356 gene-regulatory proteins Human genes 0.000 claims 2
- 108091006104 gene-regulatory proteins Proteins 0.000 claims 2
- 239000012212 insulator Substances 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 101001000998 Homo sapiens Protein phosphatase 1 regulatory subunit 12C Proteins 0.000 claims 1
- 102100035620 Protein phosphatase 1 regulatory subunit 12C Human genes 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000004043 responsiveness Effects 0.000 claims 1
- 229960001603 tamoxifen Drugs 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562242884P | 2015-10-16 | 2015-10-16 | |
| US62/242,884 | 2015-10-16 | ||
| PCT/IB2016/001902 WO2017064566A2 (en) | 2015-10-16 | 2016-10-14 | Inducible modification of a cell genome |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2018531013A JP2018531013A (ja) | 2018-10-25 |
| JP2018531013A6 JP2018531013A6 (ja) | 2018-12-13 |
| JP2018531013A5 true JP2018531013A5 (enExample) | 2019-11-28 |
| JP6936952B2 JP6936952B2 (ja) | 2021-09-22 |
Family
ID=58054378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018518522A Active JP6936952B2 (ja) | 2015-10-16 | 2016-10-14 | 細胞ゲノムの誘導性改変 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10947559B2 (enExample) |
| EP (1) | EP3362561A2 (enExample) |
| JP (1) | JP6936952B2 (enExample) |
| CN (1) | CN108431225A (enExample) |
| AU (1) | AU2016337408B2 (enExample) |
| CA (1) | CA3001518A1 (enExample) |
| WO (1) | WO2017064566A2 (enExample) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| CN108431225A (zh) * | 2015-10-16 | 2018-08-21 | 阿斯特拉捷利康股份公司 | 细胞基因组的诱导型修饰 |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| WO2017078631A1 (en) | 2015-11-05 | 2017-05-11 | Agency For Science, Technology And Research | Chemical-inducible genome engineering technology |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| KR102647165B1 (ko) * | 2017-06-16 | 2024-03-14 | 론자 리미티드 | 생물 제제 생산을 위한 범용 자체 조절 포유동물 세포주 플랫폼 |
| KR101928659B1 (ko) * | 2017-06-19 | 2018-12-13 | 서울대학교병원 | Dna 변형 유도 단백질 복합체 및 이를 암호화하는 유전자를 포함하는 재조합 벡터 |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| WO2020028533A1 (en) * | 2018-08-01 | 2020-02-06 | Yale University | Compositions and methods for identification of membrane targets for enhancement of t cell activity against cancer |
| US20210381001A1 (en) * | 2018-10-11 | 2021-12-09 | Eidgenössische Technische Hochschule Zürich | A method to treat disease using a nucleic acid vector encoding a highly compact multi-input logic gate |
| US11851663B2 (en) * | 2018-10-14 | 2023-12-26 | Snipr Biome Aps | Single-vector type I vectors |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001306T5 (de) | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| EP4069855A1 (en) * | 2019-12-04 | 2022-10-12 | CRISPR Therapeutics AG | Regulatable expression systems |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
| CN111705080A (zh) * | 2020-05-14 | 2020-09-25 | 广州市第八人民医院 | Hbv非人动物模型的构建方法及其应用 |
| CN116262927B (zh) * | 2021-12-13 | 2024-04-26 | 中国科学院微生物研究所 | 基于CRISPR/Cas系统调控基因表达的方法及其应用 |
| CN121100182A (zh) | 2023-05-11 | 2025-12-09 | 阿斯利康(瑞典)有限公司 | 治疗慢性肾脏疾病的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5928906A (en) | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
| WO2005123916A2 (en) | 2004-06-21 | 2005-12-29 | Novo Nordisk Health Care Ag | Glycosylation-disrupted factor vii variants |
| JP4811765B2 (ja) * | 2004-06-22 | 2011-11-09 | 学校法人東海大学 | 外来遺伝子の誘導発現の制御が可能な発現ベクター |
| EP2877213B1 (en) * | 2012-07-25 | 2020-12-02 | The Broad Institute, Inc. | Inducible dna binding proteins and genome perturbation tools and applications thereof |
| EP4234696A3 (en) * | 2012-12-12 | 2023-09-06 | The Broad Institute Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
| CN108431225A (zh) * | 2015-10-16 | 2018-08-21 | 阿斯特拉捷利康股份公司 | 细胞基因组的诱导型修饰 |
-
2016
- 2016-10-14 CN CN201680074288.4A patent/CN108431225A/zh active Pending
- 2016-10-14 CA CA3001518A patent/CA3001518A1/en active Pending
- 2016-10-14 AU AU2016337408A patent/AU2016337408B2/en active Active
- 2016-10-14 EP EP16837979.0A patent/EP3362561A2/en active Pending
- 2016-10-14 JP JP2018518522A patent/JP6936952B2/ja active Active
- 2016-10-14 US US15/768,482 patent/US10947559B2/en active Active
- 2016-10-14 WO PCT/IB2016/001902 patent/WO2017064566A2/en not_active Ceased
-
2021
- 2021-02-03 US US17/166,823 patent/US20210230630A1/en active Pending
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