JP2018523826A5 - - Google Patents
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- Publication number
- JP2018523826A5 JP2018523826A5 JP2018506118A JP2018506118A JP2018523826A5 JP 2018523826 A5 JP2018523826 A5 JP 2018523826A5 JP 2018506118 A JP2018506118 A JP 2018506118A JP 2018506118 A JP2018506118 A JP 2018506118A JP 2018523826 A5 JP2018523826 A5 JP 2018523826A5
- Authority
- JP
- Japan
- Prior art keywords
- ligand
- antigen
- sample
- binding complex
- stranded oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000427 antigen Substances 0.000 claims 50
- 108091007433 antigens Proteins 0.000 claims 50
- 102000036639 antigens Human genes 0.000 claims 50
- 239000003446 ligand Substances 0.000 claims 50
- 108091034117 Oligonucleotide Proteins 0.000 claims 43
- 239000012530 fluid Substances 0.000 claims 32
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 20
- 238000000034 method Methods 0.000 claims 19
- 239000000243 solution Substances 0.000 claims 15
- 108090001008 Avidin Proteins 0.000 claims 10
- 108010090804 Streptavidin Proteins 0.000 claims 10
- 229960002685 biotin Drugs 0.000 claims 10
- 235000020958 biotin Nutrition 0.000 claims 10
- 239000011616 biotin Substances 0.000 claims 10
- 238000003384 imaging method Methods 0.000 claims 9
- 239000006059 cover glass Substances 0.000 claims 6
- 238000009396 hybridization Methods 0.000 claims 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims 6
- -1 2-nitrobenzyl group Chemical group 0.000 claims 4
- 238000004891 communication Methods 0.000 claims 4
- 230000021615 conjugation Effects 0.000 claims 4
- 108091023037 Aptamer Proteins 0.000 claims 3
- 239000007853 buffer solution Substances 0.000 claims 3
- 238000003776 cleavage reaction Methods 0.000 claims 3
- 230000007017 scission Effects 0.000 claims 3
- 239000003638 chemical reducing agent Substances 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- 238000010186 staining Methods 0.000 claims 2
- PZBFGYYEXUXCOF-UHFFFAOYSA-N TCEP Chemical group OC(=O)CCP(CCC(O)=O)CCC(O)=O PZBFGYYEXUXCOF-UHFFFAOYSA-N 0.000 claims 1
- ISAOCJYIOMOJEB-UHFFFAOYSA-N benzoin Chemical group C=1C=CC=CC=1C(O)C(=O)C1=CC=CC=C1 ISAOCJYIOMOJEB-UHFFFAOYSA-N 0.000 claims 1
- 238000001574 biopsy Methods 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 125000000332 coumarinyl group Chemical group O1C(=O)C(=CC2=CC=CC=C12)* 0.000 claims 1
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 239000000975 dye Substances 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 239000003531 protein hydrolysate Substances 0.000 claims 1
- 230000005855 radiation Effects 0.000 claims 1
- 230000002285 radioactive effect Effects 0.000 claims 1
- VUFNRPJNRFOTGK-UHFFFAOYSA-M sodium;1-[4-[(2,5-dioxopyrrol-1-yl)methyl]cyclohexanecarbonyl]oxy-2,5-dioxopyrrolidine-3-sulfonate Chemical group [Na+].O=C1C(S(=O)(=O)[O-])CC(=O)N1OC(=O)C1CCC(CN2C(C=CC2=O)=O)CC1 VUFNRPJNRFOTGK-UHFFFAOYSA-M 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562201978P | 2015-08-06 | 2015-08-06 | |
| US62/201,978 | 2015-08-06 | ||
| PCT/US2016/045981 WO2017024298A1 (en) | 2015-08-06 | 2016-08-08 | Antigen detection using photocleavable labels |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018523826A JP2018523826A (ja) | 2018-08-23 |
| JP2018523826A5 true JP2018523826A5 (enExample) | 2019-10-17 |
| JP6893914B2 JP6893914B2 (ja) | 2021-06-23 |
Family
ID=56686978
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018506118A Active JP6893914B2 (ja) | 2015-08-06 | 2016-08-08 | 光開裂可能標識を使用する抗原検出の方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US10942176B2 (enExample) |
| EP (1) | EP3332253A1 (enExample) |
| JP (1) | JP6893914B2 (enExample) |
| CN (1) | CN107949790B (enExample) |
| AU (2) | AU2016304599A1 (enExample) |
| BR (1) | BR112018002525A2 (enExample) |
| CA (2) | CA2993993C (enExample) |
| MX (1) | MX2018001519A (enExample) |
| WO (1) | WO2017024298A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110545847A (zh) * | 2017-04-25 | 2019-12-06 | 达纳-法伯癌症研究所有限公司 | 用于肿瘤的靶向免疫疗法的组合物和方法 |
| EP3431173A1 (en) * | 2017-07-19 | 2019-01-23 | Bayer Pharma Aktiengesellschaft | Continuous manufacture of guidance molecule drug conjugates |
| WO2019152391A1 (en) * | 2018-01-30 | 2019-08-08 | Rush University Medical Center | Sequential staining for multiplex analyses of tissues and cells |
| US20210228699A1 (en) * | 2018-06-04 | 2021-07-29 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for targeted immunotherapy |
| CN113272443B (zh) | 2019-01-07 | 2025-01-10 | 安捷伦科技有限公司 | 用于单细胞中的基因组dna和基因表达分析的组合物和方法 |
| JP2020143015A (ja) * | 2019-03-06 | 2020-09-10 | ソニー株式会社 | 粒子標識用試薬、粒子染色方法、及び顕微鏡システム |
| WO2020252488A2 (en) * | 2019-06-14 | 2020-12-17 | Akoya Biosciences, Inc. | Multiplexed tissue imaging |
| CN111381034A (zh) * | 2020-03-03 | 2020-07-07 | 武汉生之源生物科技股份有限公司 | 一种gpc-3抗体包被磁珠及其制备方法、试剂盒 |
| WO2022076413A1 (en) * | 2020-10-05 | 2022-04-14 | Clara Biotech, Inc. | Apparatus, system, and method for analyte release |
| CN112595845B (zh) * | 2020-12-09 | 2022-10-21 | 深圳普门科技股份有限公司 | 透明质酸检测试剂盒及检测系统 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US41115A (en) * | 1864-01-05 | Edward b | ||
| US6358689B1 (en) | 1994-05-11 | 2002-03-19 | Boston University | Detection of markers in nascent proteins |
| US6589736B1 (en) | 1994-11-22 | 2003-07-08 | The Trustees Of Boston University | Photocleavable agents and conjugates for the detection and isolation of biomolecules |
| CN2444230Y (zh) | 2000-05-16 | 2001-08-22 | 陈智 | 免疫组织化学仪 |
| US7154598B2 (en) | 2002-07-12 | 2006-12-26 | Decision Biomarkers, Inc. | Excitation and imaging of fluorescent arrays |
| US7838283B2 (en) | 2005-04-21 | 2010-11-23 | Celerus Diagnostics, Inc. | Wicking cassette method and apparatus for automated rapid immunohistochemistry |
| US8492129B2 (en) | 2005-12-12 | 2013-07-23 | Innova Biosciences, Ltd. | Production of conjugates |
| HRP20140172T1 (hr) | 2006-05-30 | 2014-03-28 | Genentech, Inc. | Protutijela i imunokonjugati kao i njihove uporabe |
| US20090203023A1 (en) * | 2008-02-11 | 2009-08-13 | University Of Wyoming | Concentrating Microorganisms in Aqueous Solution Prior to Selective Staining and Detection |
| US20090253163A1 (en) | 2008-04-02 | 2009-10-08 | General Electric Company | Iterative staining of biological samples |
| AU2009257369B2 (en) | 2008-06-11 | 2015-03-26 | Agilent Technologies, Inc. | Nucleotides and nucleosides and methods for their use in DNA sequencing |
| DE102008062372B3 (de) * | 2008-12-17 | 2010-06-17 | Medizinische Hochschule Hannover | Nachweiskonjugat und Verfahren zur Analyse |
| KR101066247B1 (ko) * | 2009-08-04 | 2011-09-20 | 서울대학교산학협력단 | 멀티 루프 코일을 구비하는 판재용 비접촉식 트랜스듀서 |
| CN102230895B (zh) | 2011-03-31 | 2014-10-29 | 中国科学院合肥物质科学研究院 | 一种利用荧光蛋白作为荧光探针测量电离辐射下细胞氧化胁迫损伤的方法 |
| CN104024269B (zh) | 2011-09-13 | 2017-05-24 | 激光基因公司 | 5‑甲氧基，3’‑oh未阻断，可快速光切割的终止核苷酸以及用于核酸测序的方法 |
| KR101969847B1 (ko) * | 2012-10-30 | 2019-04-17 | 삼성전자주식회사 | 동일 검체에서 여러 항원을 검출하기 위한 순차적 다중 면역염색법 |
| CA2940764C (en) | 2014-02-26 | 2019-10-29 | Nelson Alexander | Photo-selective method for biological sample analysis field |
| US10640816B2 (en) | 2015-07-17 | 2020-05-05 | Nanostring Technologies, Inc. | Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue |
-
2016
- 2016-08-08 WO PCT/US2016/045981 patent/WO2017024298A1/en not_active Ceased
- 2016-08-08 CN CN201680051653.XA patent/CN107949790B/zh active Active
- 2016-08-08 BR BR112018002525A patent/BR112018002525A2/pt not_active Application Discontinuation
- 2016-08-08 US US15/230,876 patent/US10942176B2/en active Active
- 2016-08-08 EP EP16751795.2A patent/EP3332253A1/en active Pending
- 2016-08-08 MX MX2018001519A patent/MX2018001519A/es unknown
- 2016-08-08 CA CA2993993A patent/CA2993993C/en active Active
- 2016-08-08 AU AU2016304599A patent/AU2016304599A1/en not_active Abandoned
- 2016-08-08 JP JP2018506118A patent/JP6893914B2/ja active Active
- 2016-08-08 CA CA3138794A patent/CA3138794C/en active Active
-
2021
- 2021-02-12 US US17/174,860 patent/US20210181191A1/en not_active Abandoned
-
2022
- 2022-08-01 AU AU2022210222A patent/AU2022210222B2/en active Active
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