CA3138794C - Antigen detection using photocleavable labels - Google Patents

Antigen detection using photocleavable labels Download PDF

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Publication number
CA3138794C
CA3138794C CA3138794A CA3138794A CA3138794C CA 3138794 C CA3138794 C CA 3138794C CA 3138794 A CA3138794 A CA 3138794A CA 3138794 A CA3138794 A CA 3138794A CA 3138794 C CA3138794 C CA 3138794C
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CA
Canada
Prior art keywords
antigen
sample
fluid
photocleavable
ligand
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Active
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CA3138794A
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English (en)
French (fr)
Other versions
CA3138794A1 (en
Inventor
Mimi Healy
Xiyi CHEN
Jinchun Wang
Weidong Wu
Sophia PETRICHENKO
Su Zhang
Stacie FRYE
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Agilent Technologies Inc
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Agilent Technologies Inc
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Publication of CA3138794A1 publication Critical patent/CA3138794A1/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/082—Active control of flow resistance, e.g. flow controllers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/302—Stain compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00138—Slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3138794A 2015-08-06 2016-08-08 Antigen detection using photocleavable labels Active CA3138794C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562201978P 2015-08-06 2015-08-06
US62/201,978 2015-08-06
CA2993993A CA2993993C (en) 2015-08-06 2016-08-08 Antigen detection using photocleavable labels

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2993993A Division CA2993993C (en) 2015-08-06 2016-08-08 Antigen detection using photocleavable labels

Publications (2)

Publication Number Publication Date
CA3138794A1 CA3138794A1 (en) 2017-02-09
CA3138794C true CA3138794C (en) 2024-01-23

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CA3138794A Active CA3138794C (en) 2015-08-06 2016-08-08 Antigen detection using photocleavable labels
CA2993993A Active CA2993993C (en) 2015-08-06 2016-08-08 Antigen detection using photocleavable labels

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2993993A Active CA2993993C (en) 2015-08-06 2016-08-08 Antigen detection using photocleavable labels

Country Status (9)

Country Link
US (2) US10942176B2 (enExample)
EP (1) EP3332253A1 (enExample)
JP (1) JP6893914B2 (enExample)
CN (1) CN107949790B (enExample)
AU (2) AU2016304599A1 (enExample)
BR (1) BR112018002525A2 (enExample)
CA (2) CA3138794C (enExample)
MX (1) MX2018001519A (enExample)
WO (1) WO2017024298A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
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US20200306376A1 (en) * 2017-04-25 2020-10-01 Dana-Farber Cancer Institute, Inc. Compositions and methods for targeted tumor immunotherapy
EP3431173A1 (en) * 2017-07-19 2019-01-23 Bayer Pharma Aktiengesellschaft Continuous manufacture of guidance molecule drug conjugates
WO2019152391A1 (en) 2018-01-30 2019-08-08 Rush University Medical Center Sequential staining for multiplex analyses of tissues and cells
US20210228699A1 (en) * 2018-06-04 2021-07-29 Dana-Farber Cancer Institute, Inc. Compositions and methods for targeted immunotherapy
US11739321B2 (en) 2019-01-07 2023-08-29 Agilent Technologies, Inc. Compositions and methods for genomic DNA and gene expression analysis in single cells
JP2020143015A (ja) * 2019-03-06 2020-09-10 ソニー株式会社 粒子標識用試薬、粒子染色方法、及び顕微鏡システム
CN114556074A (zh) * 2019-06-14 2022-05-27 阿科亚生物科学股份有限公司 多重组织成像
CN111381034A (zh) * 2020-03-03 2020-07-07 武汉生之源生物科技股份有限公司 一种gpc-3抗体包被磁珠及其制备方法、试剂盒
WO2022076413A1 (en) * 2020-10-05 2022-04-14 Clara Biotech, Inc. Apparatus, system, and method for analyte release
CN112595845B (zh) * 2020-12-09 2022-10-21 深圳普门科技股份有限公司 透明质酸检测试剂盒及检测系统

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US41115A (en) * 1864-01-05 Edward b
US6358689B1 (en) 1994-05-11 2002-03-19 Boston University Detection of markers in nascent proteins
US6589736B1 (en) 1994-11-22 2003-07-08 The Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
CN2444230Y (zh) * 2000-05-16 2001-08-22 陈智 免疫组织化学仪
US7154598B2 (en) * 2002-07-12 2006-12-26 Decision Biomarkers, Inc. Excitation and imaging of fluorescent arrays
WO2006116037A2 (en) 2005-04-21 2006-11-02 Celerus Diagnostics, Inc. Enhanced fluidic method and apparatus for automated rapid immunohistochemistry
EP1968641B1 (en) 2005-12-12 2014-06-11 Innova Biosciences Limited Production of conjugates
NZ597251A (en) 2006-05-30 2013-08-30 Genentech Inc Antibodies and immunoconjugates and uses therefor
US20090203023A1 (en) * 2008-02-11 2009-08-13 University Of Wyoming Concentrating Microorganisms in Aqueous Solution Prior to Selective Staining and Detection
US20090253163A1 (en) 2008-04-02 2009-10-08 General Electric Company Iterative staining of biological samples
NZ590265A (en) 2008-06-11 2012-11-30 Lasergen Inc Nucleotides and nucleosides and methods for their use in dna sequencing
DE102008062372B3 (de) * 2008-12-17 2010-06-17 Medizinische Hochschule Hannover Nachweiskonjugat und Verfahren zur Analyse
KR101066247B1 (ko) * 2009-08-04 2011-09-20 서울대학교산학협력단 멀티 루프 코일을 구비하는 판재용 비접촉식 트랜스듀서
CN102230895B (zh) * 2011-03-31 2014-10-29 中国科学院合肥物质科学研究院 一种利用荧光蛋白作为荧光探针测量电离辐射下细胞氧化胁迫损伤的方法
EP3670523B1 (en) 2011-09-13 2021-08-11 Agilent Technologies, Inc. 3'-oh unblocked, fast photocleavable terminating nucleotides and their use in methods for nucleic acid sequencing
KR101969847B1 (ko) * 2012-10-30 2019-04-17 삼성전자주식회사 동일 검체에서 여러 항원을 검출하기 위한 순차적 다중 면역염색법
JP6608381B2 (ja) * 2014-02-26 2019-11-20 ヴェンタナ メディカル システムズ， インク． 生物学的試料分析のための光選択的方法

Also Published As

Publication number Publication date
US20210181191A1 (en) 2021-06-17
AU2022210222A1 (en) 2022-08-25
CN107949790A (zh) 2018-04-20
CA2993993C (en) 2022-01-04
JP6893914B2 (ja) 2021-06-23
EP3332253A1 (en) 2018-06-13
MX2018001519A (es) 2018-08-01
US20170052175A1 (en) 2017-02-23
BR112018002525A2 (pt) 2018-09-25
AU2022210222B2 (en) 2025-09-18
CN107949790B (zh) 2024-07-26
US10942176B2 (en) 2021-03-09
CA2993993A1 (en) 2017-02-09
JP2018523826A (ja) 2018-08-23
CA3138794A1 (en) 2017-02-09
AU2016304599A1 (en) 2018-02-15
WO2017024298A1 (en) 2017-02-09

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