JP2018523118A5 - - Google Patents
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- JP2018523118A5 JP2018523118A5 JP2017567746A JP2017567746A JP2018523118A5 JP 2018523118 A5 JP2018523118 A5 JP 2018523118A5 JP 2017567746 A JP2017567746 A JP 2017567746A JP 2017567746 A JP2017567746 A JP 2017567746A JP 2018523118 A5 JP2018523118 A5 JP 2018523118A5
- Authority
- JP
- Japan
- Prior art keywords
- flow channel
- sample
- blood cells
- spiral
- wall
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims 22
- 239000000523 sample Substances 0.000 claims 16
- 210000000440 neutrophil Anatomy 0.000 claims 15
- 210000000601 blood cell Anatomy 0.000 claims 11
- 210000003743 erythrocyte Anatomy 0.000 claims 5
- 239000003642 reactive oxygen metabolite Substances 0.000 claims 4
- 210000004369 blood Anatomy 0.000 claims 3
- 239000008280 blood Substances 0.000 claims 3
- 239000013068 control sample Substances 0.000 claims 3
- 206010012601 diabetes mellitus Diseases 0.000 claims 3
- 239000012530 fluid Substances 0.000 claims 3
- 208000027866 inflammatory disease Diseases 0.000 claims 3
- 239000011859 microparticle Substances 0.000 claims 3
- 238000005096 rolling process Methods 0.000 claims 3
- 101001046686 Homo sapiens Integrin alpha-M Proteins 0.000 claims 2
- 102100022338 Integrin alpha-M Human genes 0.000 claims 2
- 102100034925 P-selectin glycoprotein ligand 1 Human genes 0.000 claims 2
- 101710137390 P-selectin glycoprotein ligand 1 Proteins 0.000 claims 2
- 239000012470 diluted sample Substances 0.000 claims 2
- 238000007865 diluting Methods 0.000 claims 2
- 230000003834 intracellular effect Effects 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 210000004698 lymphocyte Anatomy 0.000 claims 2
- 210000001616 monocyte Anatomy 0.000 claims 2
- 102000003800 Selectins Human genes 0.000 claims 1
- 108090000184 Selectins Proteins 0.000 claims 1
- 210000001772 blood platelet Anatomy 0.000 claims 1
- 230000003247 decreasing effect Effects 0.000 claims 1
- -1 dimethylsiloxane Chemical class 0.000 claims 1
- 230000002934 lysing effect Effects 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SG10201505259T | 2015-07-02 | ||
| SG10201505259T | 2015-07-02 | ||
| SG10201510460P | 2015-12-18 | ||
| SG10201510460P | 2015-12-18 | ||
| PCT/SG2016/050306 WO2017003380A1 (en) | 2015-07-02 | 2016-06-30 | Leukocyte and microparticles fractionation using microfluidics |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018523118A JP2018523118A (ja) | 2018-08-16 |
| JP2018523118A5 true JP2018523118A5 (https=) | 2019-06-13 |
| JP6757981B2 JP6757981B2 (ja) | 2020-09-23 |
Family
ID=57608813
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017567746A Active JP6757981B2 (ja) | 2015-07-02 | 2016-06-30 | 微少流体を用いる白血球および微小粒子分画 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US10894255B2 (https=) |
| JP (1) | JP6757981B2 (https=) |
| WO (1) | WO2017003380A1 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019086340A (ja) * | 2017-11-02 | 2019-06-06 | シスメックス株式会社 | 細胞検出方法および細胞検出システム |
| WO2019117800A1 (en) * | 2017-12-11 | 2019-06-20 | A. Menarini Biomarkers Singapore Pte Ltd | Microfluidic device and method for cell separation of a blood sample |
| EP3735281B1 (en) | 2018-01-05 | 2024-11-27 | Path Ex, Inc. | Device for the capture and removal of disease material from fluids |
| JP2021522519A (ja) * | 2018-04-19 | 2021-08-30 | スティッキーセル ピーティーワイ リミテッドＳｔｉｃｋｙｃｅｌｌ Ｐｔｙ Ｌｔｄ | 感染症の技術分野における白血球補充 |
| US12239982B2 (en) | 2018-11-14 | 2025-03-04 | The Board Of Trustees Of The Leland Stanford Junior University | Microfluidic device and diagnostic methods for allergy testing based on detection of basophil activation |
| US20220395831A1 (en) * | 2019-10-21 | 2022-12-15 | Nanyang Technological University | Direct and scalable isolation of circulating extracellular vesicles from whole blood using centrifugal forces |
| CN111060364A (zh) * | 2019-11-20 | 2020-04-24 | 天津大学 | 一种肿瘤细胞染色和筛分的集成方法及配套微流控芯片 |
| CN112973811B (zh) * | 2019-12-17 | 2022-10-18 | 香港城市大学深圳研究院 | 一种基于层流扩散的血液中外泌体富集微流控芯片 |
| CN111040928B (zh) * | 2019-12-27 | 2023-12-26 | 天津大学 | 一种用于寇氏隐甲藻处理及收集的高通量微流控芯片 |
| CN111088146A (zh) * | 2020-01-09 | 2020-05-01 | 天津大学 | 一种从胸腔积液中筛分肿瘤细胞的微流控芯片 |
| CN111774108B (zh) * | 2020-06-19 | 2021-11-12 | 东南大学 | 一种壁面分离式螺旋微流控芯片 |
| CN111778138B (zh) * | 2020-07-06 | 2022-01-11 | 中南大学 | 一种分选血浆中外泌体的微流控器件及其使用方法 |
| AU2021355552A1 (en) * | 2020-10-01 | 2023-06-01 | Carina Biotech Pty Ltd | Methods for purifying t cells |
| CN113105990A (zh) * | 2021-03-08 | 2021-07-13 | 山东师范大学 | 一种用于痰液中脱落肿瘤细胞确认及肺癌诊断的微流控装置及其应用 |
| WO2022197253A1 (en) * | 2021-03-18 | 2022-09-22 | Nanyang Technological University | Method for label-free cell activation profiling using microfluidic impedance cytometry |
| US20240033735A1 (en) * | 2022-07-29 | 2024-02-01 | Hong Kong Centre for Cerebro-Cardiovascular Health Engineering Limited | Method Of Diagnosing Cardiovascular Diseases, And A Device For Separating Components Of A Fluid Sample For Diagnosing Cardiovascular Diseases |
| WO2024148321A1 (en) * | 2023-01-06 | 2024-07-11 | University Of Kentucky Research Foundation | 3d microfluidic device and method of separating particles by size from a suspension |
| WO2025221803A1 (en) * | 2024-04-17 | 2025-10-23 | Carroll Richard J | Method of preparation of mononuclear-platelet matrix (mpm) and related apparatus |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008130977A2 (en) * | 2007-04-16 | 2008-10-30 | The General Hospital Corporation D/B/A Massachusetts General Hospital | Systems and methods for particle focusing in microchannels |
| US20110070581A1 (en) * | 2009-04-27 | 2011-03-24 | Amit Gupta | Separation of Leukocytes |
| SG178064A1 (en) * | 2009-07-21 | 2012-03-29 | Abt Holding Co | Use of stem cells to reduce leukocyte extravasation |
| US8208138B2 (en) * | 2009-09-24 | 2012-06-26 | University Of Cincinnati | Spiral microchannel particle separators, straight microchannel particle separators, and continuous particle separator and detector systems |
| US9458489B2 (en) * | 2010-03-04 | 2016-10-04 | Massachusetts Institute Of Technology | Microfluidics sorter for cell detection and isolation |
| WO2013085797A1 (en) * | 2011-12-06 | 2013-06-13 | Focus Biomedical, Llc | Method of washing cells using passive separation of a lysed blood sample with clean buffer using inertial microfluidic separation and focusing in spiral microchannels |
| WO2013181615A1 (en) | 2012-05-31 | 2013-12-05 | Massachusetts Institute Of Technology | Separation of binding molecules |
| WO2014046621A1 (en) * | 2012-09-21 | 2014-03-27 | Massachusetts Institute Of Technology | Micro-fluidic device and uses thereof |
| WO2014152643A2 (en) * | 2013-03-15 | 2014-09-25 | The Broad Institute, Inc. | Microfluidic microbe detection and isolation |
| JP6237031B2 (ja) * | 2013-09-18 | 2017-11-29 | 凸版印刷株式会社 | 成分分離方法、成分分析方法及び成分分離装置 |
| RU2016118626A (ru) * | 2013-10-16 | 2017-11-21 | Клирбридж Байомедикс Пте Лтд | Микрогидродинамический сортер для выявления и изоляции клеток |
| CN103923825B (zh) * | 2014-04-17 | 2016-01-13 | 东南大学 | 一种集成细胞分选及检测的微流控芯片系统 |
-
2016
- 2016-06-30 US US15/741,149 patent/US10894255B2/en active Active
- 2016-06-30 JP JP2017567746A patent/JP6757981B2/ja active Active
- 2016-06-30 WO PCT/SG2016/050306 patent/WO2017003380A1/en not_active Ceased
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