JP6757981B2 - 微少流体を用いる白血球および微小粒子分画 - Google Patents

微少流体を用いる白血球および微小粒子分画 Download PDF

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JP6757981B2
JP6757981B2 JP2017567746A JP2017567746A JP6757981B2 JP 6757981 B2 JP6757981 B2 JP 6757981B2 JP 2017567746 A JP2017567746 A JP 2017567746A JP 2017567746 A JP2017567746 A JP 2017567746A JP 6757981 B2 JP6757981 B2 JP 6757981B2
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JP2018523118A (ja
JP2018523118A5 (https=
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ウェイ ホウ、ハン
ウェイ ホウ、ハン
オット ベーム、ベルンハルト
オット ベーム、ベルンハルト
ミン テイ、フイ
ミン テイ、フイ
チャイ ヨアヒム ロー、セイ
チャイ ヨアヒム ロー、セイ
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Nanyang Technological University
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
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    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
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    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/042—Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism
    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/70—Mechanisms involved in disease identification
    • G01N2800/7095—Inflammation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
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  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Fluid Mechanics (AREA)
  • Zoology (AREA)
  • Ecology (AREA)
  • Physiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
JP2017567746A 2015-07-02 2016-06-30 微少流体を用いる白血球および微小粒子分画 Active JP6757981B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
SG10201505259T 2015-07-02
SG10201505259T 2015-07-02
SG10201510460P 2015-12-18
SG10201510460P 2015-12-18
PCT/SG2016/050306 WO2017003380A1 (en) 2015-07-02 2016-06-30 Leukocyte and microparticles fractionation using microfluidics

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JP2018523118A JP2018523118A (ja) 2018-08-16
JP2018523118A5 JP2018523118A5 (https=) 2019-06-13
JP6757981B2 true JP6757981B2 (ja) 2020-09-23

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JP2019086340A (ja) * 2017-11-02 2019-06-06 シスメックス株式会社 細胞検出方法および細胞検出システム
WO2019117800A1 (en) * 2017-12-11 2019-06-20 A. Menarini Biomarkers Singapore Pte Ltd Microfluidic device and method for cell separation of a blood sample
EP3735281B1 (en) 2018-01-05 2024-11-27 Path Ex, Inc. Device for the capture and removal of disease material from fluids
JP2021522519A (ja) * 2018-04-19 2021-08-30 スティッキーセル ピーティーワイ リミテッドＳｔｉｃｋｙｃｅｌｌ Ｐｔｙ Ｌｔｄ 感染症の技術分野における白血球補充
US12239982B2 (en) 2018-11-14 2025-03-04 The Board Of Trustees Of The Leland Stanford Junior University Microfluidic device and diagnostic methods for allergy testing based on detection of basophil activation
US20220395831A1 (en) * 2019-10-21 2022-12-15 Nanyang Technological University Direct and scalable isolation of circulating extracellular vesicles from whole blood using centrifugal forces
CN111060364A (zh) * 2019-11-20 2020-04-24 天津大学 一种肿瘤细胞染色和筛分的集成方法及配套微流控芯片
CN112973811B (zh) * 2019-12-17 2022-10-18 香港城市大学深圳研究院 一种基于层流扩散的血液中外泌体富集微流控芯片
CN111040928B (zh) * 2019-12-27 2023-12-26 天津大学 一种用于寇氏隐甲藻处理及收集的高通量微流控芯片
CN111088146A (zh) * 2020-01-09 2020-05-01 天津大学 一种从胸腔积液中筛分肿瘤细胞的微流控芯片
CN111774108B (zh) * 2020-06-19 2021-11-12 东南大学 一种壁面分离式螺旋微流控芯片
CN111778138B (zh) * 2020-07-06 2022-01-11 中南大学 一种分选血浆中外泌体的微流控器件及其使用方法
AU2021355552A1 (en) * 2020-10-01 2023-06-01 Carina Biotech Pty Ltd Methods for purifying t cells
CN113105990A (zh) * 2021-03-08 2021-07-13 山东师范大学 一种用于痰液中脱落肿瘤细胞确认及肺癌诊断的微流控装置及其应用
WO2022197253A1 (en) * 2021-03-18 2022-09-22 Nanyang Technological University Method for label-free cell activation profiling using microfluidic impedance cytometry
US20240033735A1 (en) * 2022-07-29 2024-02-01 Hong Kong Centre for Cerebro-Cardiovascular Health Engineering Limited Method Of Diagnosing Cardiovascular Diseases, And A Device For Separating Components Of A Fluid Sample For Diagnosing Cardiovascular Diseases
WO2024148321A1 (en) * 2023-01-06 2024-07-11 University Of Kentucky Research Foundation 3d microfluidic device and method of separating particles by size from a suspension
WO2025221803A1 (en) * 2024-04-17 2025-10-23 Carroll Richard J Method of preparation of mononuclear-platelet matrix (mpm) and related apparatus

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US20180185846A1 (en) 2018-07-05
WO2017003380A1 (en) 2017-01-05
US10894255B2 (en) 2021-01-19

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