JP2018518181A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2018518181A5 JP2018518181A5 JP2017565262A JP2017565262A JP2018518181A5 JP 2018518181 A5 JP2018518181 A5 JP 2018518181A5 JP 2017565262 A JP2017565262 A JP 2017565262A JP 2017565262 A JP2017565262 A JP 2017565262A JP 2018518181 A5 JP2018518181 A5 JP 2018518181A5
- Authority
- JP
- Japan
- Prior art keywords
- complex
- cell precursor
- nucleotide sequence
- supercharged protein
- specific nuclease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims description 47
- 210000000173 T-lymphoid precursor cell Anatomy 0.000 claims description 36
- 101710163270 Nuclease Proteins 0.000 claims description 34
- 102000004169 proteins and genes Human genes 0.000 claims description 33
- 108090000623 proteins and genes Proteins 0.000 claims description 33
- 239000002773 nucleotide Substances 0.000 claims description 28
- 125000003729 nucleotide group Chemical group 0.000 claims description 28
- 108020004414 DNA Proteins 0.000 claims description 18
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 claims description 17
- 108020005004 Guide RNA Proteins 0.000 claims description 14
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims description 12
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims description 12
- 239000005090 green fluorescent protein Substances 0.000 claims description 12
- 230000006798 recombination Effects 0.000 claims description 10
- 238000005215 recombination Methods 0.000 claims description 10
- 210000004027 cell Anatomy 0.000 claims description 9
- 206010028980 Neoplasm Diseases 0.000 claims description 8
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 5
- 230000004570 RNA-binding Effects 0.000 claims description 5
- 230000005782 double-strand break Effects 0.000 claims description 5
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 claims description 5
- 150000007523 nucleic acids Chemical group 0.000 claims description 5
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 5
- 108091033409 CRISPR Proteins 0.000 claims description 4
- 239000012190 activator Substances 0.000 claims description 4
- 230000027455 binding Effects 0.000 claims description 4
- 210000004899 c-terminal region Anatomy 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 4
- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 claims description 4
- 230000035897 transcription Effects 0.000 claims description 4
- 238000013518 transcription Methods 0.000 claims description 4
- 230000010354 integration Effects 0.000 claims description 3
- 238000002054 transplantation Methods 0.000 claims description 3
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 2
- 201000011510 cancer Diseases 0.000 claims description 2
- 230000004069 differentiation Effects 0.000 claims description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims description 2
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 2
- 230000006780 non-homologous end joining Effects 0.000 claims description 2
- 101800004910 Nuclease A Proteins 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562181145P | 2015-06-17 | 2015-06-17 | |
| US62/181,145 | 2015-06-17 | ||
| PCT/US2016/038125 WO2016205680A1 (en) | 2015-06-17 | 2016-06-17 | Crispr/cas9 complex for introducing a functional polypeptide into cells of blood cell lineage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018518181A JP2018518181A (ja) | 2018-07-12 |
| JP2018518181A5 true JP2018518181A5 (enExample) | 2019-07-18 |
Family
ID=57546413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017565262A Pending JP2018518181A (ja) | 2015-06-17 | 2016-06-17 | 血液細胞系列の細胞に機能的ポリペプチドを導入するためのＣＲＩＳＰＲ／Ｃａｓ９複合体 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US11555207B2 (enExample) |
| EP (1) | EP3310931B1 (enExample) |
| JP (1) | JP2018518181A (enExample) |
| CN (1) | CN108026545A (enExample) |
| AU (1) | AU2016278959A1 (enExample) |
| CA (1) | CA2989858A1 (enExample) |
| WO (1) | WO2016205680A1 (enExample) |
Families Citing this family (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| EP4079847A1 (en) | 2014-07-30 | 2022-10-26 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| JP7030522B2 (ja) | 2015-05-11 | 2022-03-07 | エディタス・メディシン、インコーポレイテッド | 幹細胞における遺伝子編集のための最適化ｃｒｉｓｐｒ／ｃａｓ９システムおよび方法 |
| JP7396783B2 (ja) | 2015-06-09 | 2023-12-12 | エディタス・メディシン、インコーポレイテッド | 移植を改善するためのｃｒｉｓｐｒ／ｃａｓ関連方法および組成物 |
| CA2989831A1 (en) | 2015-06-17 | 2016-12-22 | The Uab Research Foundation | Crispr/cas9 complex for genomic editing |
| IL294014B2 (en) | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and uses thereof |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| MY202222A (en) * | 2017-02-07 | 2024-04-17 | Agency Science Tech & Res | Methods and kits for generating mimetic innate immune cells from pluripotent stem cells |
| KR102666158B1 (ko) * | 2017-02-13 | 2024-05-16 | 아시스땅스 퍼블리끄-오삐또 드 빠리 | T 세포 전구체(progenitor)의 생성 방법 |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| CN110914310A (zh) | 2017-03-10 | 2020-03-24 | 哈佛大学的校长及成员们 | 胞嘧啶至鸟嘌呤碱基编辑器 |
| GB2575930A (en) | 2017-03-23 | 2020-01-29 | Harvard College | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| AU2018283405B2 (en) | 2017-06-15 | 2025-06-26 | The Regents Of The University Of California | Targeted non-viral DNA insertions |
| WO2019014564A1 (en) | 2017-07-14 | 2019-01-17 | Editas Medicine, Inc. | SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES |
| WO2019018410A1 (en) * | 2017-07-17 | 2019-01-24 | The General Hospital Corporation | COMPOSITIONS AND METHODS FOR TREATING INFLAMMATORY BOWEL DISEASES |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| US11905513B2 (en) * | 2017-10-13 | 2024-02-20 | Mbp Titan, Llc | Advanced genome editing |
| WO2019079347A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | USES OF BASIC EDITORS ADENOSINE |
| EP3700924A4 (en) | 2017-10-27 | 2021-11-03 | The Regents of the University of California | TARGETED REPLACEMENT OF ENDOGENOUS T-CELL RECEPTORS |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US20210254049A1 (en) * | 2018-04-20 | 2021-08-19 | Cellino Biotech, Inc. | Directed cell fate specification and targeted maturation |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| EP4585692A3 (en) | 2018-10-16 | 2025-09-17 | BlueAllele Corporation | Methods for targeted insertion of dna in genes |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| WO2020097083A1 (en) | 2018-11-06 | 2020-05-14 | Cellino Biotech, Inc. | Systems for cell control |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001342T5 (de) | 2019-03-19 | 2022-01-13 | President and Fellows of Harvard College | Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| WO2021072328A1 (en) | 2019-10-10 | 2021-04-15 | The Broad Institute, Inc. | Methods and compositions for prime editing rna |
| GB2614813B (en) | 2020-05-08 | 2025-05-07 | Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| CA3205479A1 (en) * | 2020-12-28 | 2022-07-07 | The Regents Of The University Of California | Engineered gamma delta t cells and methods of making and using thereof |
| CR20240195A (es) | 2021-10-14 | 2024-06-20 | Arsenal Biosciences Inc | Células inmunitarias que tienen arnhc coexpresados y sistemas de compuerta lógica |
| CN117801056A (zh) * | 2023-06-29 | 2024-04-02 | 北京百普赛斯生物科技股份有限公司 | 一种从溶液中纯化目标蛋白的方法 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009097140A1 (en) * | 2008-01-30 | 2009-08-06 | Memorial Sloan-Kettering Cancer Center | Methods for off -the -shelf tumor immunotherapy using allogeneic t-cell precursors |
| JP2012525146A (ja) * | 2009-04-28 | 2012-10-22 | プレジデント アンド フェロウズ オブ ハーバード カレッジ | 細胞透過のための過剰に荷電されたタンパク質 |
| RU2018109507A (ru) | 2012-02-24 | 2019-02-28 | Фред Хатчинсон Кэнсер Рисерч Сентер | Композиции и способы лечения гемоглобинопатии |
| WO2013176772A1 (en) | 2012-05-25 | 2013-11-28 | The Regents Of The University Of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| WO2014059173A2 (en) * | 2012-10-10 | 2014-04-17 | Sangamo Biosciences, Inc. | T cell modifying compounds and uses thereof |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| US20140310830A1 (en) | 2012-12-12 | 2014-10-16 | Feng Zhang | CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes |
| CN105209621B (zh) | 2012-12-12 | 2021-05-25 | 布罗德研究所有限公司 | 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 |
| IL307735A (en) | 2012-12-12 | 2023-12-01 | Broad Inst Inc | Systems engineering, methods and optimal guiding components for sequence manipulation |
| EP4234696A3 (en) | 2012-12-12 | 2023-09-06 | The Broad Institute Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
| PL2931898T3 (pl) | 2012-12-12 | 2016-09-30 | Le Cong | Projektowanie i optymalizacja systemów, sposoby i kompozycje do manipulacji sekwencją z domenami funkcjonalnymi |
| WO2014165707A2 (en) * | 2013-04-03 | 2014-10-09 | Memorial Sloan-Kettering Cancer Center | Effective generation of tumor-targeted t-cells derived from pluripotent stem cells |
| WO2014165825A2 (en) * | 2013-04-04 | 2014-10-09 | President And Fellows Of Harvard College | Therapeutic uses of genome editing with crispr/cas systems |
| WO2014191128A1 (en) | 2013-05-29 | 2014-12-04 | Cellectis | Methods for engineering t cells for immunotherapy by using rna-guided cas nuclease system |
| WO2015006498A2 (en) | 2013-07-09 | 2015-01-15 | President And Fellows Of Harvard College | Therapeutic uses of genome editing with crispr/cas systems |
| US9526784B2 (en) * | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US12180263B2 (en) * | 2014-11-06 | 2024-12-31 | President And Fellows Of Harvard College | Cells lacking B2M surface expression and methods for allogeneic administration of such cells |
| WO2016094880A1 (en) * | 2014-12-12 | 2016-06-16 | The Broad Institute Inc. | Delivery, use and therapeutic applications of crispr systems and compositions for genome editing as to hematopoietic stem cells (hscs) |
| WO2016123578A1 (en) | 2015-01-30 | 2016-08-04 | The Regents Of The University Of California | Protein delivery in primary hematopoietic cells |
| CN107847524A (zh) | 2015-03-27 | 2018-03-27 | 哈佛学院校长同事会 | 经过修饰的t细胞及其制备和使用方法 |
| CN104894068A (zh) * | 2015-05-04 | 2015-09-09 | 南京凯地生物科技有限公司 | 一种利用CRISPR/Cas9制备CAR-T细胞的方法 |
| EP3443075B1 (en) * | 2016-04-15 | 2022-09-28 | Memorial Sloan Kettering Cancer Center | Transgenic t cell and chimeric antigen receptor t cell compositions and related methods |
-
2016
- 2016-06-17 JP JP2017565262A patent/JP2018518181A/ja active Pending
- 2016-06-17 AU AU2016278959A patent/AU2016278959A1/en not_active Abandoned
- 2016-06-17 EP EP16812542.5A patent/EP3310931B1/en active Active
- 2016-06-17 WO PCT/US2016/038125 patent/WO2016205680A1/en not_active Ceased
- 2016-06-17 CA CA2989858A patent/CA2989858A1/en not_active Abandoned
- 2016-06-17 CN CN201680044599.6A patent/CN108026545A/zh active Pending
- 2016-06-17 US US15/737,134 patent/US11555207B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2018518181A5 (enExample) | ||
| JP2018518182A5 (enExample) | ||
| JP2022097517A (ja) | リンパ球における高レベル且つ安定な遺伝子移入のための方法 | |
| US20240175046A1 (en) | Vectors | |
| CN106916820B (zh) | 能有效编辑猪ROSA26基因的sgRNA及其应用 | |
| CN113302292B (zh) | 遗传修饰细胞的减少和最少的操作制造 | |
| Waddington et al. | A broad overview and review of CRISPR-Cas technology and stem cells | |
| JP2017513477A5 (enExample) | ||
| JP2016518142A5 (enExample) | ||
| CN106701830A (zh) | 一种敲除猪胚胎p66shc基因的方法 | |
| KR20200108927A (ko) | 변형된 줄기세포 기억 t 세포, 이의 제조 방법 및 사용 방법 | |
| US20250075233A1 (en) | Multiplex editing with cas enzymes | |
| CN104212836A (zh) | 一种在哺乳动物细胞系中敲除mir-505的方法 | |
| WO2019086007A1 (zh) | 靶向并引导Cas9蛋白高效切割TCR及B2M基因座的sgRNA | |
| CN103725712A (zh) | 一种无物种限制的条件性基因敲除用中间载体及其制备方法和用途 | |
| WO2022093846A1 (en) | Safe harbor loci | |
| CN109929865B (zh) | 基于gal4-uas系统的crispr辅助反式增强子激活基因表达的方法及其应用 | |
| CN104388456A (zh) | 一种同时表达两条sgRNA的载体的构建方法 | |
| JP2025518552A (ja) | 細胞を操作するための組成物及び方法 | |
| EP4370676A2 (en) | Compositions and methods for targeting, editing or modifying human genes | |
| US20250115903A1 (en) | Compositions and methods for editing genomes | |
| US12091669B2 (en) | Method for the generation of dumbbell-shaped DNA vectors | |
| EP4323514A1 (en) | Non-viral homology mediated end joining | |
| JP2021170976A (ja) | 単細胞性真核紅藻への遺伝子導入方法 | |
| WO2025049877A1 (en) | Chemo-sensitive dominant clone for adaptive therapy |