JP2018513685A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2018513685A5 JP2018513685A5 JP2017553115A JP2017553115A JP2018513685A5 JP 2018513685 A5 JP2018513685 A5 JP 2018513685A5 JP 2017553115 A JP2017553115 A JP 2017553115A JP 2017553115 A JP2017553115 A JP 2017553115A JP 2018513685 A5 JP2018513685 A5 JP 2018513685A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- complementary region
- region
- mir
- strand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091070501 miRNA Proteins 0.000 claims description 17
- 239000002679 microRNA Substances 0.000 claims description 17
- 230000000295 complement effect Effects 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 13
- 108020004707 nucleic acids Proteins 0.000 claims description 12
- 150000007523 nucleic acids Chemical class 0.000 claims description 12
- 102000039446 nucleic acids Human genes 0.000 claims description 12
- 230000000903 blocking effect Effects 0.000 claims 14
- 108090000364 Ligases Proteins 0.000 claims 4
- 102000003960 Ligases Human genes 0.000 claims 4
- 239000000758 substrate Substances 0.000 claims 4
- 238000000137 annealing Methods 0.000 claims 3
- -1 mir-15a Proteins 0.000 claims 3
- 238000003757 reverse transcription PCR Methods 0.000 claims 3
- 108010061982 DNA Ligases Proteins 0.000 claims 1
- 101710086015 RNA ligase Proteins 0.000 claims 1
- 230000006154 adenylylation Effects 0.000 claims 1
- 108091031326 miR-15b stem-loop Proteins 0.000 claims 1
- 108091027943 miR-16 stem-loop Proteins 0.000 claims 1
- 108091046553 miR-26 stem-loop Proteins 0.000 claims 1
- 108091023821 miR-26-1 stem-loop Proteins 0.000 claims 1
- 108091045094 miR-26-2 stem-loop Proteins 0.000 claims 1
- 108091030938 miR-424 stem-loop Proteins 0.000 claims 1
- 108091052738 miR-486-1 stem-loop Proteins 0.000 claims 1
- 108091030654 miR-486-2 stem-loop Proteins 0.000 claims 1
- 108091063340 miR-497 stem-loop Proteins 0.000 claims 1
- 238000009396 hybridization Methods 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562146015P | 2015-04-10 | 2015-04-10 | |
| US62/146,015 | 2015-04-10 | ||
| PCT/US2016/026846 WO2016164866A1 (en) | 2015-04-10 | 2016-04-10 | METHOD FOR BLOCKING miRNA |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018513685A JP2018513685A (ja) | 2018-05-31 |
| JP2018513685A5 true JP2018513685A5 (https=) | 2020-07-02 |
| JP6791875B2 JP6791875B2 (ja) | 2020-11-25 |
Family
ID=57072747
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017553115A Active JP6791875B2 (ja) | 2015-04-10 | 2016-04-10 | ｍｉＲＮＡをブロックするための方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10480023B2 (https=) |
| EP (1) | EP3280805B1 (https=) |
| JP (1) | JP6791875B2 (https=) |
| AU (1) | AU2016246820B2 (https=) |
| CA (1) | CA2982369A1 (https=) |
| IL (1) | IL254972B (https=) |
| MX (1) | MX386064B (https=) |
| WO (1) | WO2016164866A1 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160319278A1 (en) | 2015-04-03 | 2016-11-03 | University Of Massachusetts | Fully stabilized asymmetric sirna |
| US11396650B2 (en) | 2015-06-02 | 2022-07-26 | Children's Medical Center Corporation | Nucleic acid complexes for screening barcoded compounds |
| US12077807B2 (en) | 2015-06-27 | 2024-09-03 | The Research Foundation For The State University Of New York | Compositions and methods for analyte detection using nanoswitches |
| WO2017030973A1 (en) | 2015-08-14 | 2017-02-23 | University Of Massachusetts | Bioactive conjugates for oligonucleotide delivery |
| US10478503B2 (en) | 2016-01-31 | 2019-11-19 | University Of Massachusetts | Branched oligonucleotides |
| US10287625B2 (en) * | 2016-03-18 | 2019-05-14 | Norgen Biotek Corp. | Methods and kits for separating nucleic acids by size |
| US12123869B2 (en) | 2016-03-23 | 2024-10-22 | Children's Medical Center Corporation | Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods |
| CA3033368A1 (en) | 2016-08-12 | 2018-02-15 | University Of Massachusetts | Conjugated oligonucleotides |
| US12252507B2 (en) | 2016-12-05 | 2025-03-18 | Children's Medical Center Corporation | Nucleic acid nanoswitch construction methods |
| WO2018157100A1 (en) * | 2017-02-27 | 2018-08-30 | Bioo Scientific Corporation | Methods and kits for depleting undesired nucleic acids |
| JP7406793B2 (ja) | 2017-06-23 | 2023-12-28 | ユニバーシティー オブ マサチューセッツ | ２テイル自己デリバリー型ｓｉＲＮＡおよび関連方法 |
| EP3681895A4 (en) * | 2017-09-13 | 2021-05-26 | Becton, Dickinson and Company | METHODS AND COMPOSITIONS FOR EXTRACTION OF NUCLEIC ACIDS USING FERROUS PARTICLES |
| US11591636B2 (en) | 2017-11-20 | 2023-02-28 | Children's Medical Center Corporation | Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids |
| CN108676846B (zh) * | 2018-05-25 | 2022-04-01 | 艾吉泰康生物科技(北京)有限公司 | 一种桥式寡核苷酸在文库目标区域捕获中的应用 |
| WO2020033899A1 (en) | 2018-08-10 | 2020-02-13 | University Of Massachusetts | Modified oligonucleotides targeting snps |
| US11279930B2 (en) | 2018-08-23 | 2022-03-22 | University Of Massachusetts | O-methyl rich fully stabilized oligonucleotides |
| CN113614232A (zh) * | 2019-01-18 | 2021-11-05 | 马萨诸塞大学 | 动态药代动力学修饰锚 |
| KR20220047989A (ko) | 2019-08-09 | 2022-04-19 | 유니버시티 오브 매사추세츠 | Snp를 표적화하는 화학적으로 변형된 올리고뉴클레오타이드 |
| US12365894B2 (en) | 2019-09-16 | 2025-07-22 | University Of Massachusetts | Branched lipid conjugates of siRNA for specific tissue delivery |
| WO2021174071A1 (en) | 2020-02-29 | 2021-09-02 | President And Fellows Of Harvard College | Nanoswitch caliper trains for high-throughput, high-resolution structural analysis of biomolecules |
| WO2021242883A1 (en) | 2020-05-26 | 2021-12-02 | University Of Massachusetts | Synthetic oligonucleotides having regions of block and cluster modifications |
| CN117677699A (zh) | 2021-06-23 | 2024-03-08 | 马萨诸塞大学 | 用于治疗先兆子痫和其他血管生成病症的优化抗flt1寡核苷酸化合物 |
| CN118139978A (zh) * | 2021-10-22 | 2024-06-04 | 蜂鸟诊断有限责任公司 | 使用反义寡核苷酸靶向抑制逆转录 |
| US20230323341A1 (en) * | 2022-03-25 | 2023-10-12 | Bioo Scientific Corporation | Methods, compositions, and kits for inhibiting formation of adapter dimers |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1253205A1 (en) | 2001-04-24 | 2002-10-30 | LION Bioscience AG | Method of blocking amplification of selected sequences |
| US20030211483A1 (en) * | 2002-05-09 | 2003-11-13 | Schroeder Benjamin G. | Methods for the enrichment of low-abundance polynucleotides |
| EP1924713B1 (en) | 2005-08-24 | 2011-11-09 | Life Technologies Corporation | A method to quantify sirnas, mirnas and polymorphic mirnas |
| WO2010126913A1 (en) | 2009-04-27 | 2010-11-04 | Gen-Probe Incorporated | Methods and kits for use in the selective amplification of target sequences |
| CA2779689A1 (en) * | 2009-11-06 | 2011-05-12 | Enzymatics, Inc. | Composition and method for synthesizing a deoxyribonucleotide chain using a double stranded nucleic acid complex with a thermostable polymerase |
| EP2542696B1 (en) * | 2010-03-01 | 2016-09-28 | Caris Life Sciences Switzerland Holdings GmbH | Biomarkers for theranostics |
| EP2591106A1 (en) | 2010-07-06 | 2013-05-15 | InteRNA Technologies B.V. | Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway |
| EP3620533B1 (en) * | 2011-09-06 | 2023-01-18 | Gen-Probe Incorporated | Closed nucleic acid structures |
| DK2914741T3 (da) * | 2012-11-02 | 2017-11-20 | Life Technologies Corp | Hidtil ukendte sammensætninger og fremgangsmåder til forbedring af PCR-specificitet |
-
2016
- 2016-04-10 WO PCT/US2016/026846 patent/WO2016164866A1/en not_active Ceased
- 2016-04-10 EP EP16777458.7A patent/EP3280805B1/en active Active
- 2016-04-10 US US15/565,652 patent/US10480023B2/en active Active
- 2016-04-10 MX MX2017013022A patent/MX386064B/es unknown
- 2016-04-10 JP JP2017553115A patent/JP6791875B2/ja active Active
- 2016-04-10 CA CA2982369A patent/CA2982369A1/en active Pending
- 2016-04-10 AU AU2016246820A patent/AU2016246820B2/en active Active
-
2017
- 2017-10-10 IL IL254972A patent/IL254972B/en active IP Right Grant
-
2019
- 2019-09-24 US US16/581,129 patent/US11781176B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2018513685A5 (https=) | ||
| US20240229105A1 (en) | Attenuators | |
| US20180216166A1 (en) | Attenuators | |
| EP2546361B1 (en) | Method of amplifying target nucleic acid with reduced amplification bias and method for determining relative amount of target nucleic acid in sample | |
| JP2016533187A5 (https=) | ||
| IL274464B2 (en) | Preparations and methods for the detection of rare sequence variants | |
| JP2012520080A5 (https=) | ||
| JP2015533296A5 (https=) | ||
| DE602005020691D1 (de) | Rna-amplifikation auf ligationsbasis | |
| JP2017510244A5 (https=) | ||
| JP2017515484A5 (https=) | ||
| WO2012003374A3 (en) | Targeted sequencing library preparation by genomic dna circularization | |
| JP2016537990A5 (https=) | ||
| WO2006102309A3 (en) | Methods, compositions, and kits for detection of micro rna | |
| JP2005503802A5 (https=) | ||
| CN105463066B (zh) | 一种dna扩增方法 | |
| JP2017532044A5 (https=) | ||
| JP2017521999A (ja) | プライマーダイマーの増幅を低減する方法 | |
| JP2024521142A5 (https=) | ||
| US10801056B2 (en) | Kit and method for detection of microRNA | |
| WO2015167972A4 (en) | Methods for nucleic acid base determination | |
| JP7611897B2 (ja) | 核酸配列の多重等温増幅のための方法 | |
| CN106811461A (zh) | 一种构建植物中pre‑miRNA5’RACE‑seq文库的方法 | |
| JP2018534942A5 (https=) | ||
| JP2012050393A (ja) | 核酸等温増幅方法 |