JP2018500905A - ナノチューブを使用した核酸ポリメラーゼ立体構造変化の検出 - Google Patents

ナノチューブを使用した核酸ポリメラーゼ立体構造変化の検出 Download PDF

Info

Publication number
JP2018500905A
JP2018500905A JP2017532800A JP2017532800A JP2018500905A JP 2018500905 A JP2018500905 A JP 2018500905A JP 2017532800 A JP2017532800 A JP 2017532800A JP 2017532800 A JP2017532800 A JP 2017532800A JP 2018500905 A JP2018500905 A JP 2018500905A
Authority
JP
Japan
Prior art keywords
nucleic acid
thio
nucleotide
molecule
acid polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017532800A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018500905A5 (cg-RX-API-DMAC7.html
Inventor
コリンズ，フィリップ・ジー
ワス，グレゴリー・エイ
チョイ，ヨンキ
オルセン，チボリ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California, University of California San Diego UCSD filed Critical University of California
Publication of JP2018500905A publication Critical patent/JP2018500905A/ja
Publication of JP2018500905A5 publication Critical patent/JP2018500905A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)
    • G01N2333/9126—DNA-directed DNA polymerase (2.7.7.7)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Nanotechnology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2017532800A 2014-12-18 2015-12-17 ナノチューブを使用した核酸ポリメラーゼ立体構造変化の検出 Pending JP2018500905A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462093671P 2014-12-18 2014-12-18
US62/093,671 2014-12-18
PCT/US2015/066321 WO2016100635A1 (en) 2014-12-18 2015-12-17 Detection of nucleic acid polymerase conformational changes using a nanotube

Publications (2)

Publication Number Publication Date
JP2018500905A true JP2018500905A (ja) 2018-01-18
JP2018500905A5 JP2018500905A5 (cg-RX-API-DMAC7.html) 2019-01-31

Family

ID=56127582

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017532800A Pending JP2018500905A (ja) 2014-12-18 2015-12-17 ナノチューブを使用した核酸ポリメラーゼ立体構造変化の検出

Country Status (10)

Country Link
US (1) US20180051316A1 (cg-RX-API-DMAC7.html)
EP (1) EP3234178A4 (cg-RX-API-DMAC7.html)
JP (1) JP2018500905A (cg-RX-API-DMAC7.html)
KR (1) KR20170091158A (cg-RX-API-DMAC7.html)
CN (2) CN109517878A (cg-RX-API-DMAC7.html)
AU (1) AU2015364602B2 (cg-RX-API-DMAC7.html)
CA (1) CA2971268A1 (cg-RX-API-DMAC7.html)
RU (1) RU2721965C2 (cg-RX-API-DMAC7.html)
SG (1) SG11201704862UA (cg-RX-API-DMAC7.html)
WO (2) WO2016100635A1 (cg-RX-API-DMAC7.html)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021524281A (ja) * 2018-05-17 2021-09-13 リンジー，スチュアート 酵素活性の直接電気測定用のデバイス、システムおよび方法
US12351855B2 (en) 2019-01-30 2025-07-08 Arizona Board Of Regents On Behalf Of Arizona State University Bioelectronic circuits, systems and methods for preparing and using them
US12509720B2 (en) 2021-04-30 2025-12-30 Arizona Board Of Regents On Behalf Of Arizona State University Methods for sequencing biopolymers

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102796872B1 (ko) 2016-01-28 2025-04-15 로스웰 엠이 아이엔씨. 대규모 분자 전자소자 센서 어레이들을 이용하여 분석물들을 측정하는 방법들 및 장치
JP7080489B2 (ja) 2016-01-28 2022-06-06 ロズウェル バイオテクノロジーズ，インコーポレイテッド 超パラレルｄｎａ配列決定装置
JP6854532B2 (ja) 2016-02-09 2021-04-07 ロズウェル バイオテクノロジーズ，インコーポレイテッド 電子的、標識フリーのｄｎａおよびゲノムシークエンシング
US10597767B2 (en) 2016-02-22 2020-03-24 Roswell Biotechnologies, Inc. Nanoparticle fabrication
US9829456B1 (en) 2016-07-26 2017-11-28 Roswell Biotechnologies, Inc. Method of making a multi-electrode structure usable in molecular sensing devices
CN106244712B (zh) 2016-08-31 2019-11-19 北京大学 Dna测序方法
US20190376925A1 (en) * 2016-11-22 2019-12-12 Roswell Biotechnologies, Inc. Nucleic acid sequencing device containing graphene
KR102622275B1 (ko) 2017-01-10 2024-01-05 로스웰 바이오테크놀로지스 인코포레이티드 Dna 데이터 저장을 위한 방법들 및 시스템들
KR102601324B1 (ko) * 2017-01-19 2023-11-10 로스웰 바이오테크놀로지스 인코포레이티드 2차원 레이어 재료를 포함하는 솔리드 스테이트 시퀀싱 디바이스들
US10508296B2 (en) 2017-04-25 2019-12-17 Roswell Biotechnologies, Inc. Enzymatic circuits for molecular sensors
WO2018200687A1 (en) * 2017-04-25 2018-11-01 Roswell Biotechnologies, Inc. Enzymatic circuits for molecular sensors
EP4023764A3 (en) 2017-05-09 2022-09-21 Roswell Biotechnologies, Inc. Binding probe circuits for molecular sensors
EP3676389A4 (en) 2017-08-30 2021-06-02 Roswell Biotechnologies, Inc PROCESSIVE ENZYMATIC MOLECULAR ELECTRONIC SENSORS FOR STORING DNA DATA
KR20210135344A (ko) * 2017-09-15 2021-11-12 일루미나, 인코포레이티드 서열-검출 시스템의 조율 및 교정 특징
WO2019055867A1 (en) * 2017-09-15 2019-03-21 Illumina, Inc. SEQUENCE DETECTION SYSTEM
KR102717872B1 (ko) * 2017-10-10 2024-10-14 로스웰 엠이 아이엔씨. 무증폭 dna 데이터 저장을 위한 방법, 장치 및 시스템
KR20230169413A (ko) 2017-10-13 2023-12-15 노보 노르디스크 헬스 케어 악티엔게젤샤프트 Ldha의 발현을 억제하기 위한 방법 및 조성물
WO2020101795A1 (en) 2018-11-15 2020-05-22 Omniome, Inc. Electronic detection of nucleic acid structure
GB2585608B (en) 2018-12-04 2021-11-03 Omniome Inc Mixed-phase fluids for nucleic acid sequencing and other analytical assays
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
US20220145377A1 (en) * 2019-03-01 2022-05-12 Universal Sequencing Technology Corporation Device and Method for Biopolymer Identification
KR20220012920A (ko) * 2019-05-27 2022-02-04 유니버셜 시퀀싱 테크놀로지 코퍼레이션 핵산 서열 내의 구성요소를 확인하는 방법
CN114728996B (zh) 2019-09-10 2022-11-29 加利福尼亚太平洋生物科学股份有限公司 核苷酸的可逆修饰
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
EP4100161B1 (en) 2020-02-04 2024-07-10 Pacific Biosciences of California, Inc. Flow cells and methods for their use
IL295828A (en) * 2020-02-28 2022-10-01 Univ Arizona State Methods for biopolymer flooring
US12298300B2 (en) 2020-04-17 2025-05-13 Arizona Board Of Regents On Behalf Of Arizona State University Single-molecule electronic sequence detector and methods of use
EP4158063A4 (en) 2020-05-29 2024-05-29 Arizona Board of Regents on behalf of Arizona State University BIOELECTRONIC DEVICES WITH PROGRAMMABLE ADAPTERS
EP4231174A3 (en) 2020-06-11 2023-11-01 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
KR20230118570A (ko) 2020-11-11 2023-08-11 노틸러스 서브시디어리, 인크. 강화된 결합 및 검출 특성을 갖는 친화성 시약
US20220227890A1 (en) 2021-01-21 2022-07-21 Nautilus Biotechnology, Inc. Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
CA3227872A1 (en) 2021-09-09 2023-03-16 James Henry JOLY Characterization and localization of protein modifications
AU2022352593A1 (en) 2021-09-22 2024-02-15 Nautilus Subsidiary, Inc. Methods and systems for determining polypeptide interactions
US20230149883A1 (en) 2021-11-03 2023-05-18 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
AU2023241893A1 (en) 2022-03-29 2024-09-26 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
CN117174183B (zh) * 2022-05-27 2025-11-25 中国科学院大连化学物理研究所 基于多尺度分子动力学模拟的rna结构预测方法
EP4544077A1 (en) 2022-06-21 2025-04-30 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
EP4630816A1 (en) 2022-12-09 2025-10-15 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
EP4634285A1 (en) 2022-12-15 2025-10-22 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
WO2024206122A1 (en) 2023-03-24 2024-10-03 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
WO2025128588A1 (en) 2023-12-11 2025-06-19 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
US20250205706A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130078622A1 (en) * 2011-09-26 2013-03-28 The Regents Of The University Of California Electronic device for monitoring single molecule dynamics
WO2014031575A1 (en) * 2012-08-20 2014-02-27 The Regents Of The University Of California Polynucleotides having bioreversible groups

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8911972B2 (en) * 2009-12-16 2014-12-16 Pacific Biosciences Of California, Inc. Sequencing methods using enzyme conformation
US20110287432A1 (en) * 2010-05-21 2011-11-24 454 Life Science Corporation System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
US8890091B2 (en) * 2010-06-03 2014-11-18 Element Six Limited Diamond tools
WO2012097074A2 (en) * 2011-01-11 2012-07-19 The Trustees Of Columbia University In The City Of New York Systems and methods for single-molecule detection using nanotubes
US9279154B2 (en) * 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130078622A1 (en) * 2011-09-26 2013-03-28 The Regents Of The University Of California Electronic device for monitoring single molecule dynamics
WO2014031575A1 (en) * 2012-08-20 2014-02-27 The Regents Of The University Of California Polynucleotides having bioreversible groups

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BIOCHIM BIOPHYS ACTA, 2010 (EPUB 20090729), VOL. 1804, NO. 5, PP. 1151-1166, JPN6020042500, ISSN: 0004607163 *
KAITLIN M. PUGLIESE: "PROCESSIVE INCORPORATION OF DEOXYNUCLEOSIDE TRIPHOSPHATE ANALOGS BY SINGLE-MOLECULE DNA 以下備考", J. AM. CHEM. SOC., vol. V137 N30, JPN5017010398, 17 July 2015 (2015-07-17), pages 9587 - 9594, ISSN: 0004381887 *
PROCEEDINGS OF SPIE, 2013 SEP 24, VOL. 8814, PP. 88140Q-1-9 (9 PAGES), JPN6019044898, ISSN: 0004381886 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021524281A (ja) * 2018-05-17 2021-09-13 リンジー，スチュアート 酵素活性の直接電気測定用のデバイス、システムおよび方法
JP7433300B2 (ja) 2018-05-17 2024-02-19 レコグニション アナリティクス インコーポレイテッド 酵素活性の直接電気測定用のデバイス、システムおよび方法
US12480937B2 (en) 2018-05-17 2025-11-25 Recognition AnalytiX, Inc. Device, system and method for direct electrical measurement of enzyme activity
US12351855B2 (en) 2019-01-30 2025-07-08 Arizona Board Of Regents On Behalf Of Arizona State University Bioelectronic circuits, systems and methods for preparing and using them
US12509720B2 (en) 2021-04-30 2025-12-30 Arizona Board Of Regents On Behalf Of Arizona State University Methods for sequencing biopolymers

Also Published As

Publication number Publication date
SG11201704862UA (en) 2017-07-28
AU2015364602A1 (en) 2017-07-06
RU2721965C2 (ru) 2020-05-25
WO2016100637A1 (en) 2016-06-23
EP3234178A4 (en) 2018-07-11
CA2971268A1 (en) 2016-06-23
RU2017125417A3 (cg-RX-API-DMAC7.html) 2019-05-27
CN107109466A (zh) 2017-08-29
CN109517878A (zh) 2019-03-26
RU2017125417A (ru) 2019-01-23
AU2015364602B2 (en) 2021-12-09
EP3234178A1 (en) 2017-10-25
US20180051316A1 (en) 2018-02-22
KR20170091158A (ko) 2017-08-08
WO2016100635A1 (en) 2016-06-23

Similar Documents

Publication Publication Date Title
JP2018500905A (ja) ナノチューブを使用した核酸ポリメラーゼ立体構造変化の検出
US20250251359A1 (en) Active-electrode integrated biosensor array and methods for use thereof
US20240425916A1 (en) Systems and methods for measurement and sequencing of bio-molecules
US10473639B1 (en) Control of enzyme translocation in nanopore sequencing
JP6818995B2 (ja) 電極及び化学物質の分析のための方法
Branton et al. The potential and challenges of nanopore sequencing
US10612073B2 (en) Method for constructing nucleic acid molecule
RU2679494C1 (ru) Способ безметочного одномолекулярного секвенирования ДНК и устройство для его реализации
AU2014218911A1 (en) Methods and compositions for nanostructure-based nucleic acid sequencing
CN103717752A (zh) 核酸分析法
US20130225416A1 (en) Electronic sequencing
Namani et al. Unraveling RNA conformation dynamics in mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episode syndrome with solid-state nanopores
CN112969914A (zh) 用于使用纳米孔装置检测分子的感测组合物、方法和装置
HK40005350A (en) Detection of nucleic acid polymerase conformational changes using a nanotube
US20240011086A1 (en) Electronic detection of nucleic acid structure
RU2780050C1 (ru) Способ безметочного одномолекулярного секвенирования днк и устройство для его реализации на основе зарядочувствительного сенсора
Ling The potential and challenges of nanopore sequencing
HK40049621B (zh) 对多核苷酸进行测序的方法
HK1243080A1 (en) Method of preparation of nanopore and uses thereof
HK1247253B (zh) 微孔电极及分析化学物质的方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20181214

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20181214

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191119

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200206

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200514

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20201110

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210204

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210507

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20211005

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220125

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20220125

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20220126

C11 Written invitation by the commissioner to file amendments

Free format text: JAPANESE INTERMEDIATE CODE: C11

Effective date: 20220222

C27A Decision to dismiss

Free format text: JAPANESE INTERMEDIATE CODE: C2711

Effective date: 20220412

C609 Written withdrawal of request for trial/appeal

Free format text: JAPANESE INTERMEDIATE CODE: C609

Effective date: 20220418