JP2017536104A5 - - Google Patents
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- Publication number
- JP2017536104A5 JP2017536104A5 JP2017520506A JP2017520506A JP2017536104A5 JP 2017536104 A5 JP2017536104 A5 JP 2017536104A5 JP 2017520506 A JP2017520506 A JP 2017520506A JP 2017520506 A JP2017520506 A JP 2017520506A JP 2017536104 A5 JP2017536104 A5 JP 2017536104A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- fused
- ret
- gpcr
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004169 proteins and genes Human genes 0.000 claims 90
- 108090000623 proteins and genes Proteins 0.000 claims 90
- 238000002165 resonance energy transfer Methods 0.000 claims 77
- 239000012634 fragment Substances 0.000 claims 33
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 claims 24
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 claims 24
- 229940125633 GPCR agonist Drugs 0.000 claims 16
- 210000000170 cell membrane Anatomy 0.000 claims 16
- 230000008685 targeting Effects 0.000 claims 14
- 239000003795 chemical substances by application Substances 0.000 claims 12
- 238000000034 method Methods 0.000 claims 6
- 239000003814 drug Substances 0.000 claims 5
- 229940079593 drug Drugs 0.000 claims 5
- 239000012190 activator Substances 0.000 claims 4
- 239000003112 inhibitor Substances 0.000 claims 4
- 229940125499 GPCR antagonist Drugs 0.000 claims 3
- 108091006027 G proteins Proteins 0.000 claims 2
- 102000030782 GTP binding Human genes 0.000 claims 2
- 108091000058 GTP-Binding Proteins 0.000 claims 2
- 108010047357 Luminescent Proteins Proteins 0.000 claims 2
- 102000006830 Luminescent Proteins Human genes 0.000 claims 2
- 108091006047 fluorescent proteins Proteins 0.000 claims 2
- 102000034287 fluorescent proteins Human genes 0.000 claims 2
- 230000004927 fusion Effects 0.000 claims 2
- 230000004952 protein activity Effects 0.000 claims 2
- 102100021738 Beta-adrenergic receptor kinase 1 Human genes 0.000 claims 1
- 102100037281 Beta-adrenergic receptor kinase 2 Human genes 0.000 claims 1
- 101000751445 Homo sapiens Beta-adrenergic receptor kinase 1 Proteins 0.000 claims 1
- 101000806653 Homo sapiens Beta-adrenergic receptor kinase 2 Proteins 0.000 claims 1
- 108060001084 Luciferase Proteins 0.000 claims 1
- 239000005089 Luciferase Substances 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 239000005557 antagonist Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 108010010909 olfactory G protein subunit alpha olf Proteins 0.000 claims 1
- 230000013823 prenylation Effects 0.000 claims 1
- 210000001938 protoplast Anatomy 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462063622P | 2014-10-14 | 2014-10-14 | |
| US62/063,622 | 2014-10-14 | ||
| PCT/CA2015/051032 WO2016058094A1 (en) | 2014-10-14 | 2015-10-14 | BIOSENSOR BASED ON GβΥ-INTERACTING PROTEINS TO MONITOR G-PROTEIN ACTIVATION |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017536104A JP2017536104A (ja) | 2017-12-07 |
| JP2017536104A5 true JP2017536104A5 (cg-RX-API-DMAC7.html) | 2018-10-18 |
| JP6932636B2 JP6932636B2 (ja) | 2021-09-08 |
Family
ID=55745919
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017520506A Active JP6932636B2 (ja) | 2014-10-14 | 2015-10-14 | Ｇタンパク質活性化を監視するためのＧβγ相互作用タンパク質に基づいたバイオセンサー |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US10877036B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3207052B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6932636B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN107074926B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2015333543B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2963579C (cg-RX-API-DMAC7.html) |
| DK (1) | DK3207052T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2832331T3 (cg-RX-API-DMAC7.html) |
| IL (1) | IL250949B (cg-RX-API-DMAC7.html) |
| NZ (1) | NZ729735A (cg-RX-API-DMAC7.html) |
| PL (1) | PL3207052T3 (cg-RX-API-DMAC7.html) |
| SG (1) | SG11201701895VA (cg-RX-API-DMAC7.html) |
| WO (1) | WO2016058094A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20200400567A1 (en) * | 2017-09-27 | 2020-12-24 | Peking University | Fusion polypeptide |
| JP2020537531A (ja) * | 2017-10-18 | 2020-12-24 | ユニベルシテ ドゥ モントリオール | Ｇタンパク質活性化の評価のためのシステムおよび方法 |
| WO2019076262A1 (zh) * | 2017-10-18 | 2019-04-25 | 深圳华大生命科学研究院 | 检测目标分子化合物的生物感应器及包含该生物感应器的系统 |
| US12360111B2 (en) * | 2018-12-04 | 2025-07-15 | The University Of North Carolina At Chapel Hill | G protein-coupled receptor screening systems |
| FR3092172B1 (fr) * | 2019-01-30 | 2021-02-12 | Cisbio Bioassays | Méthode pour mesurer la modulation de l’activation d’un récepteur couplé à une protéine G avec des analogues du GTP |
| CN110981943B (zh) * | 2019-12-02 | 2021-08-03 | 清华大学 | 多肽及其在制备药物中的用途和药物 |
| KR102778534B1 (ko) | 2022-10-07 | 2025-03-07 | 성균관대학교산학협력단 | G 단백질 알파 서브유닛의 알파-헬리컬 도메인의 실시간 구조 변화 확인 방법 및 시스템 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1324047C (zh) * | 2002-04-12 | 2007-07-04 | 先灵公司 | G蛋白偶联受体的配体与方法 |
| US20050042720A1 (en) | 2003-04-24 | 2005-02-24 | 7Tm Pharma A/S | Novel promiscuous G alpha protein mutants and their use |
| US7488583B2 (en) | 2003-09-25 | 2009-02-10 | Odyssey Thera, Inc. | Fragment complementation assays for G-protein-coupled receptors and their signaling pathways |
| WO2005121755A1 (en) * | 2004-06-14 | 2005-12-22 | Commonwealth Scientific And Industrial Research Organisation | Cell free g-protein coupled receptor and ligand assay |
| GB0822259D0 (en) * | 2008-12-05 | 2009-01-14 | Ge Healthcare Uk Ltd | Methods for testing binding of a ligand to a G protein- coupled receptor |
| KR101834574B1 (ko) * | 2009-01-29 | 2018-03-05 | 커먼웰쓰 사이언티픽 앤드 인더스트리얼 리서치 오가니제이션 | Ｇ 단백질 커플링 수용체 활성화 측정 |
| GB0905419D0 (en) * | 2009-03-30 | 2009-05-13 | Ge Healthcare Uk Ltd | Methods for testing ligand binding to G protein-coupled receptors |
| KR20140040674A (ko) | 2010-11-11 | 2014-04-03 | 우베 고산 가부시키가이샤 | 비수 전해액용 용기, 용기에 든 비수 전해액 및 비수 전해액의 보존 방법 |
| US20140031525A1 (en) * | 2011-01-21 | 2014-01-30 | Heptares Therapeutics Limited | Mutant g-protein coupled receptor proteins and methods for producing them |
| CA2970012C (en) * | 2013-12-23 | 2022-07-19 | Universite De Montreal | Systems and methods for the monitoring of protein complex formation in cells |
-
2015
- 2015-10-14 WO PCT/CA2015/051032 patent/WO2016058094A1/en not_active Ceased
- 2015-10-14 ES ES15851395T patent/ES2832331T3/es active Active
- 2015-10-14 CN CN201580055471.5A patent/CN107074926B/zh active Active
- 2015-10-14 DK DK15851395.2T patent/DK3207052T3/da active
- 2015-10-14 US US15/518,888 patent/US10877036B2/en active Active
- 2015-10-14 CA CA2963579A patent/CA2963579C/en active Active
- 2015-10-14 JP JP2017520506A patent/JP6932636B2/ja active Active
- 2015-10-14 PL PL15851395T patent/PL3207052T3/pl unknown
- 2015-10-14 SG SG11201701895VA patent/SG11201701895VA/en unknown
- 2015-10-14 AU AU2015333543A patent/AU2015333543B2/en active Active
- 2015-10-14 NZ NZ729735A patent/NZ729735A/en unknown
- 2015-10-14 EP EP15851395.2A patent/EP3207052B1/en active Active
-
2017
- 2017-03-05 IL IL250949A patent/IL250949B/en unknown
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