JP2017534285A5 - - Google Patents

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JP2017534285A5
JP2017534285A5 JP2017523864A JP2017523864A JP2017534285A5 JP 2017534285 A5 JP2017534285 A5 JP 2017534285A5 JP 2017523864 A JP2017523864 A JP 2017523864A JP 2017523864 A JP2017523864 A JP 2017523864A JP 2017534285 A5 JP2017534285 A5 JP 2017534285A5
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Japan
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scaffold
compatible end
end element
vector
crispr guide
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JP2017523864A
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JP2017534285A (ja
JP6788584B2 (ja
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Priority claimed from PCT/US2015/058304 external-priority patent/WO2016070037A2/en
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JP2017523864A 2014-10-31 2015-10-30 Ｃｒｉｓｐｒについての超並列コンビナトリアル遺伝学 Active JP6788584B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462073126P 2014-10-31 2014-10-31
US62/073,126 2014-10-31
US201562166302P 2015-05-26 2015-05-26
US62/166,302 2015-05-26
PCT/US2015/058304 WO2016070037A2 (en) 2014-10-31 2015-10-30 Massively parallel combinatorial genetics for crispr

Publications (3)

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JP2017534285A JP2017534285A (ja) 2017-11-24
JP2017534285A5 true JP2017534285A5 (enExample) 2018-12-13
JP6788584B2 JP6788584B2 (ja) 2020-11-25

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JP2017523864A Active JP6788584B2 (ja) 2014-10-31 2015-10-30 Ｃｒｉｓｐｒについての超並列コンビナトリアル遺伝学

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US (2) US20190100769A1 (enExample)
EP (2) EP3708155A1 (enExample)
JP (1) JP6788584B2 (enExample)
CN (1) CN107429246B (enExample)
DK (1) DK3212789T3 (enExample)
TW (1) TWI716367B (enExample)
WO (1) WO2016070037A2 (enExample)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
EP4063503A1 (en) 2014-02-11 2022-09-28 The Regents of the University of Colorado, a body corporate Crispr enabled multiplexed genome engineering
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
WO2016089433A1 (en) 2014-12-03 2016-06-09 Agilent Technologies, Inc. Guide rna with chemical modifications
KR102888521B1 (ko) 2015-04-06 2025-11-19 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 Crispr/cas-매개 유전자 조절을 위한 화학적으로 변형된 가이드 rna
SG10202104041PA (en) 2015-10-23 2021-06-29 Harvard College Nucleobase editors and uses thereof
IL304088A (en) 2016-01-11 2023-08-01 Univ Leland Stanford Junior Systems containing chimeric proteins and their uses for controlling gene expression
MX2018008345A (es) 2016-01-11 2018-12-06 Univ Leland Stanford Junior Proteínas quiméricas y métodos de inmunoterapia.
JP2019514377A (ja) * 2016-04-26 2019-06-06 マサチューセッツ インスティテュート オブ テクノロジー 拡張可能なリコンビナーゼカスケード
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
ES2915562T3 (es) * 2016-06-24 2022-06-23 Univ Colorado Regents Métodos para generar bibliotecas combinatorias con código de barras
US11913017B2 (en) * 2016-06-29 2024-02-27 The Regents Of The University Of California Efficient genetic screening method
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
CN109863246A (zh) 2016-08-19 2019-06-07 怀特黑德生物医学研究所 编辑dna甲基化的方法
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
WO2018049075A1 (en) * 2016-09-07 2018-03-15 Flagship Pioneering, Inc. Methods and compositions for modulating gene expression
SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
WO2018111944A1 (en) 2016-12-12 2018-06-21 Whitehead Institute For Biomedical Research Regulation of transcription through ctcf loop anchors
US10815525B2 (en) * 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
AU2017385611B2 (en) * 2016-12-29 2023-11-02 Johann Wolfgang Goethe-Universität Frankfurt am Main Method for generating higher order genome editing libraries
FI3565891T3 (fi) 2017-01-09 2023-07-04 Whitehead Inst Biomedical Res Menetelmiä geeniekspression muuttamiseksi häiritsemällä säätelysilmukoita muodostavia transkriptiotekijämultimeerejä
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
WO2018145041A1 (en) * 2017-02-06 2018-08-09 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
WO2018176009A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
CA3068072A1 (en) 2017-07-31 2019-02-07 Regeneron Pharmaceuticals, Inc. Methods and compositions for assessing crispr/cas-mediated disruption or excision and crispr/cas-induced recombination with an exogenous donor nucleic acid in vivo
CN110891420B (zh) 2017-07-31 2022-06-03 瑞泽恩制药公司 Cas转基因小鼠胚胎干细胞和小鼠及其应用
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
WO2019089803A1 (en) * 2017-10-31 2019-05-09 The Broad Institute, Inc. Methods and compositions for studying cell evolution
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
WO2020033585A1 (en) * 2018-08-07 2020-02-13 The Broad Institute, Inc. Methods for combinatorial screening and use of therapeutic targets thereof
US20230193251A1 (en) * 2018-09-19 2023-06-22 The University Of Hong Kong Improved high-throughput combinatorial genetic modification system and optimized cas9 enzyme variants
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
WO2020125762A1 (en) * 2018-12-20 2020-06-25 Peking University Compositions and methods for highly efficient genetic screening using barcoded guide rna constructs
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
EP3942043A2 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
AU2020352552A1 (en) 2019-09-23 2022-03-17 Omega Therapeutics, Inc. Compositions and methods for modulating hepatocyte nuclear factor 4-alpha (HNF4α) gene expression
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
CN110652589A (zh) * 2019-10-12 2020-01-07 中国人民解放军陆军军医大学 Gasc1抑制剂在制备治疗肝癌的药物中的应用
WO2021119435A1 (en) * 2019-12-12 2021-06-17 The Trustees Of The University Of Pennsylvania High performance platform for combinatorial genetic screening
US20230227814A1 (en) 2020-04-16 2023-07-20 The University Of Hong Kong System for three-way combinatorial crispr screens for analysing target interactions and methods thereof
BR112022022603A2 (pt) 2020-05-08 2023-01-17 Broad Inst Inc Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla
US20240110175A1 (en) * 2021-01-19 2024-04-04 The Board Of Trustees Of The Leland Stanford Junior University Composition and method for high-multiplexed genome engineering using synthetic crispr arrays
WO2022195582A1 (en) * 2021-03-15 2022-09-22 G.T.A.I Innovation Ltd. A method and apparatus for lab tests
CN113599522B (zh) * 2021-08-03 2022-09-20 深圳市北科生物科技有限公司 Kdm6作为靶标在制备用于提高早期神经外胚层分化效率的药物中的应用
WO2023039586A1 (en) 2021-09-10 2023-03-16 Agilent Technologies, Inc. Guide rnas with chemical modification for prime editing
CN116019811B (zh) * 2023-02-07 2024-04-12 华中科技大学协和深圳医院 Gsk-j4用于制备抗革兰氏阳性菌感染药物中的应用
CN118425525B (zh) * 2024-03-13 2025-08-22 郑州大学第一附属医院 Kdm5b在肾纤维化治疗中的应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2564039A1 (en) * 2004-05-10 2005-11-17 Basf Plant Science Gmbh Methods for assembling multiple expression constructs
US8476260B2 (en) * 2007-12-28 2013-07-02 Mitsubishi Tanabe Pharma Corporation Antitumor agent
MX373121B (es) * 2010-05-14 2020-04-30 Dana Farber Cancer Inst Inc Composiciones y metodos para el tratamiento de leucemia.
AR084070A1 (es) * 2010-12-02 2013-04-17 Constellation Pharmaceuticals Inc Inhibidores del bromodominio y usos de los mismos
LT3401400T (lt) 2012-05-25 2019-06-10 The Regents Of The University Of California Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui
CN103516916B (zh) 2012-06-28 2018-03-27 中兴通讯股份有限公司 拨号方法及装置
CN104508130B (zh) * 2012-06-29 2016-09-28 麻省理工学院 大规模并行组合遗传学
BR112015007083A2 (pt) * 2012-10-02 2017-07-04 Epitherapeutics Aps inibidores de histona demetilases
US9073878B2 (en) * 2012-11-21 2015-07-07 Zenith Epigenetics Corp. Cyclic amines as bromodomain inhibitors
CN103833671B (zh) * 2012-11-27 2016-12-21 中国科学院上海药物研究所 一类二氢噻唑酮类化合物及其药物组合物和用途
EP3135765A1 (en) * 2012-12-06 2017-03-01 Sigma-Aldrich Co. LLC Crispr-based genome modification and regulation
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
IL239317B (en) * 2012-12-12 2022-07-01 Broad Inst Inc Providing, engineering and optimizing systems, methods and compositions for sequence manipulation and therapeutic applications
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
SG11201507378UA (en) * 2013-03-14 2015-10-29 Caribou Biosciences Inc Compositions and methods of nucleic acid-targeting nucleic acids
EP2978757B1 (en) 2013-03-27 2017-02-08 Boehringer Ingelheim International GmbH Indolinone analogues as brd4 inhibitors
JP6738729B2 (ja) * 2013-06-17 2020-08-12 ザ・ブロード・インスティテュート・インコーポレイテッド 分裂終了細胞の疾患および障害をターゲティングおよびモデリングするための系、方法および組成物の送達、エンジニアリングおよび最適化
RU2016128077A (ru) * 2013-12-12 2018-12-06 Те Брод Инститьют Инк. Доставка, применение и применения в терапии систем и композиций crispr-cas для лечения обусловленных hbv и вирусных заболеваний и нарушений
CN111206032B (zh) * 2013-12-12 2024-07-19 布罗德研究所有限公司 用于基因组编辑的crispr-cas系统和组合物的递送、用途和治疗应用
BR112016013213A2 (pt) * 2013-12-12 2017-12-05 Massachusetts Inst Technology administração, uso e aplicações terapêuticas dos sistemas crispr-cas e composições para visar distúrbios e doenças usando componentes de administração de partículas
WO2015095501A1 (en) * 2013-12-18 2015-06-25 Onn Brandman Pooled method for high throughput screening of trans factors affecting rna levels
EP3126503A1 (en) * 2014-04-03 2017-02-08 Massachusetts Institute Of Technology Methods and compositions for the production of guide rna
EP3137079A1 (en) * 2014-04-28 2017-03-08 The J. David Gladstone Institutes Inhibitors of jmjd2c as anticancer agents
CA2956962A1 (en) * 2014-08-13 2016-02-18 Epizyme, Inc. Combination therapy for treating cancer
WO2016049024A2 (en) * 2014-09-24 2016-03-31 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling competition of multiple cancer mutations in vivo

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