CN107429246B - 用于crispr的大规模并行组合遗传学 - Google Patents

用于crispr的大规模并行组合遗传学 Download PDF

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CN107429246B
CN107429246B CN201580065962.8A CN201580065962A CN107429246B CN 107429246 B CN107429246 B CN 107429246B CN 201580065962 A CN201580065962 A CN 201580065962A CN 107429246 B CN107429246 B CN 107429246B
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CN107429246A (zh
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卢冠达
黄兆麟
蔡正姿
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Massachusetts Institute of Technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/4709—Non-condensed quinolines and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
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    • A61K31/00—Medicinal preparations containing organic active ingredients
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/55—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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CN201580065962.8A 2014-10-31 2015-10-30 用于crispr的大规模并行组合遗传学 Active CN107429246B (zh)

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US201462073126P 2014-10-31 2014-10-31
US62/073,126 2014-10-31
US201562166302P 2015-05-26 2015-05-26
US62/166,302 2015-05-26
PCT/US2015/058304 WO2016070037A2 (en) 2014-10-31 2015-10-30 Massively parallel combinatorial genetics for crispr

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TWI716367B (zh) 2021-01-21
CN107429246A (zh) 2017-12-01
EP3212789B1 (en) 2020-04-22
WO2016070037A3 (en) 2016-06-23
EP3708155A1 (en) 2020-09-16
JP2017534285A (ja) 2017-11-24
JP6788584B2 (ja) 2020-11-25
EP3212789A2 (en) 2017-09-06
US20210310022A1 (en) 2021-10-07
WO2016070037A8 (en) 2017-03-02
US20190100769A1 (en) 2019-04-04
DK3212789T3 (da) 2020-07-27
WO2016070037A2 (en) 2016-05-06
HK1243456A1 (en) 2018-07-13
TW201631155A (zh) 2016-09-01

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