JP2017530718A5 - - Google Patents
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- Publication number
- JP2017530718A5 JP2017530718A5 JP2017520418A JP2017520418A JP2017530718A5 JP 2017530718 A5 JP2017530718 A5 JP 2017530718A5 JP 2017520418 A JP2017520418 A JP 2017520418A JP 2017520418 A JP2017520418 A JP 2017520418A JP 2017530718 A5 JP2017530718 A5 JP 2017530718A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- recombinant expression
- expression vector
- nucleic acid
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000003259 recombinant expression Methods 0.000 claims 10
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 9
- 150000007523 nucleic acids Chemical group 0.000 claims 7
- 230000001105 regulatory Effects 0.000 claims 6
- 101710037135 GAPC2 Proteins 0.000 claims 3
- 101710037116 GAPC3 Proteins 0.000 claims 3
- 101710025049 GAPDG Proteins 0.000 claims 3
- 101710008404 GAPDH Proteins 0.000 claims 3
- 102100006425 GAPDH Human genes 0.000 claims 3
- 101710010461 Gapdh1 Proteins 0.000 claims 3
- 101710025050 MK0970 Proteins 0.000 claims 3
- 101710025091 cbbGC Proteins 0.000 claims 3
- 101710025070 gapdh-2 Proteins 0.000 claims 3
- 241000699800 Cricetinae Species 0.000 claims 1
- 125000002485 formyl group Chemical group [H]C(*)=O 0.000 claims 1
Claims (10)
- 外因性目的遺伝子に機能的に連結されたハムスターＧＡＰＤＨプロモーターを含む発現カセットを含む、組換え発現ベクターであって、
（ａ）配列番号３５に記載の核酸配列または配列番号３８に記載の核酸配列に少なくとも９５％同一である核酸配列を含み、及び
（ｂ）前記プロモーターに機能的に連結された
調節エレメントをさらに含む、組換え発現ベクター。 - 前記ＧＡＰＤＨプロモーターが配列番号５０のヌクレオチド配列を含む、請求項１に記載の組換え発現ベクター。
- 前記ＧＡＰＤＨプロモーターの３’側かつ前記目的遺伝子の５’側に、配列番号５２のヌクレオチド配列を含む、請求項１または２に記載の組換え発現ベクター。
- 前記調節エレメントが、配列番号３５または配列番号３８と少なくとも９８％同一である核酸配列を含む、請求項１〜３のいずれか一項に記載の組換え発現ベクター。
- 前記調節エレメントが、配列番号３５または配列番号３８と少なくとも９９％同一である核酸配列を含む、請求項１〜４のいずれか一項に記載の組換え発現ベクター。
- 前記調節エレメントが、配列番号３５の核酸配列を含む、請求項１〜５のいずれか一項に記載の組換え発現ベクター。
- 前記調節エレメントが、配列番号３８の核酸配列を含む、請求項１〜５のいずれか一項に記載の組換え発現ベクター。
- 前記調節エレメントがプラス方向である、請求項１〜７のいずれか一項に記載の組換え発現ベクター。
- 請求項１〜８のいずれか一項に記載の組換え発現ベクターを含む、哺乳動物宿主細胞。
- 前記細胞がＣＨＯ細胞である、請求項９に記載の哺乳動物宿主細胞。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462064335P | 2014-10-15 | 2014-10-15 | |
| US62/064,335 | 2014-10-15 | ||
| PCT/US2015/055549 WO2016061240A1 (en) | 2014-10-15 | 2015-10-14 | Promoter and regulatory elements for improved expression of heterologous genes in host cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017530718A JP2017530718A (ja) | 2017-10-19 |
| JP2017530718A5 true JP2017530718A5 (ja) | 2018-11-22 |
| JP6749898B2 JP6749898B2 (ja) | 2020-09-02 |
Family
ID=54541176
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017520418A Active JP6749898B2 (ja) | 2014-10-15 | 2015-10-14 | 宿主細胞における異種由来遺伝子の発現を改善するためのプロモーター及び調節エレメント |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US10202616B2 (ja) |
| EP (2) | EP3722433B1 (ja) |
| JP (1) | JP6749898B2 (ja) |
| AU (1) | AU2015332577B2 (ja) |
| CA (1) | CA2964313C (ja) |
| ES (2) | ES2962385T3 (ja) |
| MX (1) | MX2017004769A (ja) |
| WO (1) | WO2016061240A1 (ja) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4218799A1 (en) | 2009-07-15 | 2023-08-02 | GlaxoSmithKline Biologicals S.A. | Rsv f protein compositions and methods for making same |
| TW202233660A (zh) | 2020-10-30 | 2022-09-01 | 美商安進公司 | 過表現胰島素樣生長因子受體突變體以調節ｉｇｆ補充 |
| CN114196703B (zh) * | 2021-12-22 | 2023-02-03 | 西北农林科技大学 | 一种提高牦牛克隆胚胎发育率的载体、细胞和方法 |
Family Cites Families (44)
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|---|---|---|---|---|
| US30985A (en) | 1860-12-18 | Thomas l | ||
| USRE30985E (en) | 1978-01-01 | 1982-06-29 | Serum-free cell culture media | |
| US4560655A (en) | 1982-12-16 | 1985-12-24 | Immunex Corporation | Serum-free cell culture medium and process for making same |
| US4657866A (en) | 1982-12-21 | 1987-04-14 | Sudhir Kumar | Serum-free, synthetic, completely chemically defined tissue culture media |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US4767704A (en) | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
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| US4927762A (en) | 1986-04-01 | 1990-05-22 | Cell Enterprises, Inc. | Cell culture medium with antioxidant |
| US5567610A (en) | 1986-09-04 | 1996-10-22 | Bioinvent International Ab | Method of producing human monoclonal antibodies and kit therefor |
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| ATE135397T1 (de) | 1988-09-23 | 1996-03-15 | Cetus Oncology Corp | Zellenzuchtmedium für erhöhtes zellenwachstum, zur erhöhung der langlebigkeit und expression der produkte |
| GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| US5175384A (en) | 1988-12-05 | 1992-12-29 | Genpharm International | Transgenic mice depleted in mature t-cells and methods for making transgenic mice |
| SG48759A1 (en) | 1990-01-12 | 2002-07-23 | Abgenix Inc | Generation of xenogenic antibodies |
| US5229275A (en) | 1990-04-26 | 1993-07-20 | Akzo N.V. | In-vitro method for producing antigen-specific human monoclonal antibodies |
| US5427908A (en) | 1990-05-01 | 1995-06-27 | Affymax Technologies N.V. | Recombinant library screening methods |
| GB9014932D0 (en) | 1990-07-05 | 1990-08-22 | Celltech Ltd | Recombinant dna product and method |
| GB9015198D0 (en) | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| GB9022545D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| GB9022543D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Antibody production |
| US5565332A (en) | 1991-09-23 | 1996-10-15 | Medical Research Council | Production of chimeric antibodies - a combinatorial approach |
| WO1993016177A1 (en) | 1992-02-11 | 1993-08-19 | Cell Genesys, Inc. | Homogenotization of gene-targeting events |
| US5573905A (en) | 1992-03-30 | 1996-11-12 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
| AU675661B2 (en) | 1992-07-24 | 1997-02-13 | Abgenix, Inc. | Generation of xenogeneic antibodies |
| US6054287A (en) | 1994-05-27 | 2000-04-25 | Methodist Hospital Of Indiana, Inc. | Cell-type-specific methods and devices for the low temperature preservation of the cells of an animal species |
| US6130364A (en) | 1995-03-29 | 2000-10-10 | Abgenix, Inc. | Production of antibodies using Cre-mediated site-specific recombination |
| EP1978033A3 (en) | 1995-04-27 | 2008-12-24 | Amgen Fremont Inc. | Human antibodies derived from immunized xenomice |
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| ES2301183T3 (es) | 1996-12-03 | 2008-06-16 | Amgen Fremont Inc. | Anticuerpo completamente humano que se une al receptor del egfr. |
| US20020032315A1 (en) | 1997-08-06 | 2002-03-14 | Manuel Baca | Anti-vegf antibodies |
| US20030044772A1 (en) | 1997-08-04 | 2003-03-06 | Applied Molecular Evolution [Formerly Ixsys] | Methods for identifying ligand specific binding molecules |
| US6022952A (en) | 1998-04-01 | 2000-02-08 | University Of Alberta | Compositions and methods for protein secretion |
| US20020029391A1 (en) | 1998-04-15 | 2002-03-07 | Claude Geoffrey Davis | Epitope-driven human antibody production and gene expression profiling |
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| JP2002538841A (ja) | 1999-03-18 | 2002-11-19 | ヒューマン ジノーム サイエンシーズ， インコーポレイテッド | ２７個のヒト分泌タンパク質 |
| CA2430013C (en) | 2000-11-30 | 2011-11-22 | Medarex, Inc. | Transgenic transchromosomal rodents for making human antibodies |
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| GB0509965D0 (en) * | 2005-05-17 | 2005-06-22 | Ml Lab Plc | Improved expression elements |
| TW200722436A (en) | 2005-10-21 | 2007-06-16 | Hoffmann La Roche | A peptide-immunoglobulin-conjugate |
| EP2118270A2 (en) * | 2007-01-08 | 2009-11-18 | Millipore Corporation | Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants |
| EA037273B1 (ru) * | 2011-12-07 | 2021-03-02 | Икнос Сайенсиз С.А. | Экспрессионная кассета |
-
2015
- 2015-10-14 ES ES20173389T patent/ES2962385T3/es active Active
- 2015-10-14 MX MX2017004769A patent/MX2017004769A/es active IP Right Grant
- 2015-10-14 EP EP20173389.6A patent/EP3722433B1/en active Active
- 2015-10-14 JP JP2017520418A patent/JP6749898B2/ja active Active
- 2015-10-14 WO PCT/US2015/055549 patent/WO2016061240A1/en active Application Filing
- 2015-10-14 AU AU2015332577A patent/AU2015332577B2/en active Active
- 2015-10-14 CA CA2964313A patent/CA2964313C/en active Active
- 2015-10-14 US US15/519,820 patent/US10202616B2/en active Active
- 2015-10-14 EP EP15794369.7A patent/EP3207146B1/en active Active
- 2015-10-14 ES ES15794369T patent/ES2900331T3/es active Active
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