JP2017520580A5 - - Google Patents
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- JP2017520580A5 JP2017520580A5 JP2016575734A JP2016575734A JP2017520580A5 JP 2017520580 A5 JP2017520580 A5 JP 2017520580A5 JP 2016575734 A JP2016575734 A JP 2016575734A JP 2016575734 A JP2016575734 A JP 2016575734A JP 2017520580 A5 JP2017520580 A5 JP 2017520580A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- transposon
- target nucleic
- target
- transposome
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 150000007523 nucleic acids Chemical class 0.000 claims 77
- 108020004707 nucleic acids Proteins 0.000 claims 74
- 102000039446 nucleic acids Human genes 0.000 claims 74
- 238000000034 method Methods 0.000 claims 38
- 102000008579 Transposases Human genes 0.000 claims 14
- 108010020764 Transposases Proteins 0.000 claims 14
- 238000012163 sequencing technique Methods 0.000 claims 10
- 108020004414 DNA Proteins 0.000 claims 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 6
- 239000000523 sample Substances 0.000 claims 6
- 239000000178 monomer Substances 0.000 claims 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 3
- 238000011176 pooling Methods 0.000 claims 3
- 108010034143 Inflammasomes Proteins 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- 108090001008 Avidin Proteins 0.000 claims 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 125000000217 alkyl group Chemical group 0.000 claims 1
- 125000003277 amino group Chemical group 0.000 claims 1
- 125000003118 aryl group Chemical group 0.000 claims 1
- IVRMZWNICZWHMI-UHFFFAOYSA-N azide group Chemical group [N-]=[N+]=[N-] IVRMZWNICZWHMI-UHFFFAOYSA-N 0.000 claims 1
- 239000002299 complementary DNA Substances 0.000 claims 1
- 238000005034 decoration Methods 0.000 claims 1
- -1 different sequences Chemical class 0.000 claims 1
- 239000000539 dimer Substances 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 102000054766 genetic haplotypes Human genes 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 125000006850 spacer group Chemical group 0.000 claims 1
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 1
- 125000003396 thiol group Chemical group [H]S* 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462019209P | 2014-06-30 | 2014-06-30 | |
| US62/019,209 | 2014-06-30 | ||
| PCT/US2015/038050 WO2016003814A1 (en) | 2014-06-30 | 2015-06-26 | Methods and compositions using one-sided transposition |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017520580A JP2017520580A (ja) | 2017-07-27 |
| JP2017520580A5 true JP2017520580A5 (OSRAM) | 2018-08-02 |
| JP6652512B2 JP6652512B2 (ja) | 2020-02-26 |
Family
ID=53514440
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016575734A Active JP6652512B2 (ja) | 2014-06-30 | 2015-06-26 | 片側転移を用いた方法および組成物 |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US10577603B2 (OSRAM) |
| EP (1) | EP3161152B1 (OSRAM) |
| JP (1) | JP6652512B2 (OSRAM) |
| CN (2) | CN106661561B (OSRAM) |
| AU (1) | AU2015284464B2 (OSRAM) |
| CA (1) | CA2953791A1 (OSRAM) |
| DK (1) | DK3161152T3 (OSRAM) |
| ES (1) | ES2713153T3 (OSRAM) |
| SG (1) | SG11201610910QA (OSRAM) |
| WO (1) | WO2016003814A1 (OSRAM) |
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