AU2015284464B2 - Methods and compositions using one-sided transposition - Google Patents
Methods and compositions using one-sided transposition Download PDFInfo
- Publication number
- AU2015284464B2 AU2015284464B2 AU2015284464A AU2015284464A AU2015284464B2 AU 2015284464 B2 AU2015284464 B2 AU 2015284464B2 AU 2015284464 A AU2015284464 A AU 2015284464A AU 2015284464 A AU2015284464 A AU 2015284464A AU 2015284464 B2 AU2015284464 B2 AU 2015284464B2
- Authority
- AU
- Australia
- Prior art keywords
- nucleic acid
- nucleic acids
- transposon
- target nucleic
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462019209P | 2014-06-30 | 2014-06-30 | |
| US62/019,209 | 2014-06-30 | ||
| PCT/US2015/038050 WO2016003814A1 (en) | 2014-06-30 | 2015-06-26 | Methods and compositions using one-sided transposition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2015284464A1 AU2015284464A1 (en) | 2017-02-02 |
| AU2015284464B2 true AU2015284464B2 (en) | 2021-06-17 |
Family
ID=53514440
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2015284464A Active AU2015284464B2 (en) | 2014-06-30 | 2015-06-26 | Methods and compositions using one-sided transposition |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US10577603B2 (OSRAM) |
| EP (1) | EP3161152B1 (OSRAM) |
| JP (1) | JP6652512B2 (OSRAM) |
| CN (2) | CN106661561B (OSRAM) |
| AU (1) | AU2015284464B2 (OSRAM) |
| CA (1) | CA2953791A1 (OSRAM) |
| DK (1) | DK3161152T3 (OSRAM) |
| ES (1) | ES2713153T3 (OSRAM) |
| SG (1) | SG11201610910QA (OSRAM) |
| WO (1) | WO2016003814A1 (OSRAM) |
Families Citing this family (77)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010086622A1 (en) | 2009-01-30 | 2010-08-05 | Oxford Nanopore Technologies Limited | Adaptors for nucleic acid constructs in transmembrane sequencing |
| US9371598B2 (en) | 2010-04-05 | 2016-06-21 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| WO2013014451A1 (en) | 2011-07-25 | 2013-01-31 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
| WO2014013259A1 (en) | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Ssb method |
| EP3511423B2 (en) | 2012-10-17 | 2024-05-29 | Spatial Transcriptomics AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| SG11201507138RA (en) | 2013-03-08 | 2015-10-29 | Oxford Nanopore Tech Ltd | Enzyme stalling method |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| LT3013983T (lt) | 2013-06-25 | 2023-05-10 | Prognosys Biosciences, Inc. | Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| SG11201707515SA (en) | 2015-04-10 | 2017-10-30 | Spatial Transcriptomics Ab | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| US20170283864A1 (en) | 2016-03-31 | 2017-10-05 | Agilent Technologies, Inc. | Use of transposase and y adapters to fragment and tag dna |
| WO2017184691A1 (en) * | 2016-04-19 | 2017-10-26 | Kapa Biosystems, Inc. | Transposase competitor control system |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| US10240196B2 (en) | 2016-05-27 | 2019-03-26 | Agilent Technologies, Inc. | Transposase-random priming DNA sample preparation |
| AU2017299803B2 (en) * | 2016-07-22 | 2023-06-29 | Illumina, Inc. | Single cell whole genome libraries and combinatorial indexing methods of making thereof |
| SG11201810639RA (en) | 2017-02-21 | 2018-12-28 | Illumina Inc | Tagmentation using immobilized transposomes with linkers |
| AU2018262331A1 (en) | 2017-05-05 | 2019-11-21 | Scipio Bioscience | Methods for trapping and barcoding discrete biological units in hydrogel |
| WO2018217625A1 (en) * | 2017-05-23 | 2018-11-29 | Bio-Rad Laboratories, Inc. | Molecular barcoding |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| IL271454B2 (en) | 2018-05-17 | 2025-04-01 | Illumina Inc | Rapid single-cell genetic sequencing with low Aggregation bias |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| CN112739829A (zh) * | 2018-09-27 | 2021-04-30 | 深圳华大生命科学研究院 | 测序文库的构建方法和得到的测序文库及测序方法 |
| US12104281B2 (en) * | 2018-11-30 | 2024-10-01 | Illumina, Inc. | Analysis of multiple analytes using a single assay |
| WO2020123316A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| SG11202102424QA (en) | 2018-12-17 | 2021-04-29 | Illumina Inc | Methods and means for preparing a library for sequencing |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| AU2020266530A1 (en) | 2019-04-29 | 2021-04-08 | Illumina, Inc. | Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment |
| WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| AU2020312787A1 (en) | 2019-07-12 | 2021-06-17 | Illumina Cambridge Limited | Nucleic acid library preparation using electrophoresis |
| WO2021011803A1 (en) | 2019-07-16 | 2021-01-21 | Omniome, Inc. | Synthetic nucleic acids having non-natural structures |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| CN121022991A (zh) | 2019-12-23 | 2025-11-28 | 10X基因组学有限公司 | 使用rna模板化连接进行空间分析的方法 |
| CN115038794A (zh) | 2019-12-23 | 2022-09-09 | 10X基因组学有限公司 | 在基于分区的测定中使用固定生物样品的组合物和方法 |
| CN115667507A (zh) | 2019-12-31 | 2023-01-31 | 奇异基因组学系统公司 | 用于长读出测序的多核苷酸条形码 |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| WO2021163637A1 (en) | 2020-02-13 | 2021-08-19 | Zymergen Inc. | Metagenomic library and natural product discovery platform |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| ES2965354T3 (es) | 2020-04-22 | 2024-04-12 | 10X Genomics Inc | Métodos para análisis espacial que usan eliminación de ARN elegido como diana |
| WO2021237087A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| ES2989052T3 (es) | 2020-05-22 | 2024-11-25 | 10X Genomics Inc | Medición espacio-temporal simultánea de la expresión génica y la actividad celular |
| WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| ES2981265T3 (es) | 2020-06-08 | 2024-10-08 | 10X Genomics Inc | Métodos para determinar un margen quirúrgico y métodos de uso del mismo |
| WO2021252591A1 (en) | 2020-06-10 | 2021-12-16 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
| EP4172362B1 (en) | 2020-06-25 | 2024-09-18 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| EP4121555A1 (en) | 2020-12-21 | 2023-01-25 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| CN117222747A (zh) * | 2021-03-22 | 2023-12-12 | 伊鲁米纳剑桥有限公司 | 用于改善核酸簇克隆性的方法 |
| EP4428246B1 (en) | 2021-04-14 | 2025-12-24 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| WO2022233821A1 (en) * | 2021-05-03 | 2022-11-10 | European Molecular Biology Laboratory | Methods for isolating and/or obtaining captured polynucleotide fragments |
| WO2022236054A1 (en) | 2021-05-06 | 2022-11-10 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| EP4582555A3 (en) | 2021-06-03 | 2025-10-22 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| ES3011462T3 (en) | 2021-09-01 | 2025-04-07 | 10X Genomics Inc | Methods for blocking a capture probe on a spatial array |
| EP4419707A1 (en) | 2021-11-10 | 2024-08-28 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| EP4305195A2 (en) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| EP4453242A2 (en) * | 2021-12-23 | 2024-10-30 | Illumina, Inc. | Flow cells and methods |
| EP4272764A1 (en) | 2022-05-03 | 2023-11-08 | Scipio Bioscience | Method of complexing biological units with particles |
| WO2023225095A1 (en) * | 2022-05-18 | 2023-11-23 | Illumina Cambridge Limited | Preparation of size-controlled nucleic acid fragments |
| WO2025129074A2 (en) | 2023-12-14 | 2025-06-19 | Illumina, Inc. | Indexing techniques for tagmented dna libraries |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100120098A1 (en) * | 2008-10-24 | 2010-05-13 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| WO2012106546A2 (en) * | 2011-02-02 | 2012-08-09 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
Family Cites Families (91)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU622426B2 (en) | 1987-12-11 | 1992-04-09 | Abbott Laboratories | Assay using template-dependent nucleic acid probe reorganization |
| GB8810400D0 (en) | 1988-05-03 | 1988-06-08 | Southern E | Analysing polynucleotide sequences |
| CA1341584C (en) | 1988-04-06 | 2008-11-18 | Bruce Wallace | Method of amplifying and detecting nucleic acid sequences |
| WO1989009835A1 (en) | 1988-04-08 | 1989-10-19 | The Salk Institute For Biological Studies | Ligase-based amplification method |
| WO1989012696A1 (en) | 1988-06-24 | 1989-12-28 | Amgen Inc. | Method and reagents for detecting nucleic acid sequences |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| DE68926504T2 (de) | 1988-07-20 | 1996-09-12 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| US5573907A (en) | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
| AU635105B2 (en) | 1990-01-26 | 1993-03-11 | Abbott Laboratories | Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| JP3175110B2 (ja) | 1994-02-07 | 2001-06-11 | オーキッド・バイオサイエンシーズ・インコーポレイテッド | リガーゼ／ポリメラーゼ媒体された単一ヌクレオチド多型のジェネティックビットアナリシスおよび遺伝子解析におけるその使用 |
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| CA2185239C (en) | 1994-03-16 | 2002-12-17 | Nanibhushan Dattagupta | Isothermal strand displacement nucleic acid amplification |
| US5552278A (en) | 1994-04-04 | 1996-09-03 | Spectragen, Inc. | DNA sequencing by stepwise ligation and cleavage |
| US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
| DK0796341T3 (da) | 1994-12-09 | 1999-06-14 | Imp College Innovations Ltd | Identifikation af gener, som tillader tilpasning af en mikroorganisme til en særlig omgivelse |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5925545A (en) | 1996-09-09 | 1999-07-20 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| US5965443A (en) | 1996-09-09 | 1999-10-12 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| DE69837913T2 (de) | 1997-04-01 | 2008-02-07 | Solexa Ltd., Saffron Walden | Verfahren zur vervielfältigung von nukleinsäure |
| EP0975802B1 (en) | 1997-04-01 | 2004-06-23 | Manteia S.A. | Method of nucleic acid sequencing |
| FI103809B (fi) | 1997-07-14 | 1999-09-30 | Finnzymes Oy | In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US20050244870A1 (en) | 1999-04-20 | 2005-11-03 | Illumina, Inc. | Nucleic acid sequencing using microsphere arrays |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7611869B2 (en) | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| CN100462433C (zh) | 2000-07-07 | 2009-02-18 | 维西根生物技术公司 | 实时序列测定 |
| EP1354064A2 (en) | 2000-12-01 | 2003-10-22 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| AR031640A1 (es) | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
| US20040110191A1 (en) | 2001-01-31 | 2004-06-10 | Winkler Matthew M. | Comparative analysis of nucleic acids using population tagging |
| US7138267B1 (en) | 2001-04-04 | 2006-11-21 | Epicentre Technologies Corporation | Methods and compositions for amplifying DNA clone copy number |
| US20050130147A1 (en) * | 2001-10-09 | 2005-06-16 | Cpoyrat Pty. Ltd. | Methods of preparing a targeting vector and uses thereof |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US20040002090A1 (en) | 2002-03-05 | 2004-01-01 | Pascal Mayer | Methods for detecting genome-wide sequence variations associated with a phenotype |
| DK3002289T3 (en) | 2002-08-23 | 2018-04-23 | Illumina Cambridge Ltd | MODIFIED NUCLEOTIDES FOR POLYNUCLEOTIDE SEQUENCE |
| US7595883B1 (en) | 2002-09-16 | 2009-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Biological analysis arrangement and approach therefor |
| US7083980B2 (en) | 2003-04-17 | 2006-08-01 | Wisconsin Alumni Research Foundation | Tn5 transposase mutants and the use thereof |
| AU2004253882B2 (en) | 2003-06-20 | 2010-06-10 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| EP1682680B2 (en) | 2003-10-31 | 2018-03-21 | AB Advanced Genetic Analysis Corporation | Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof |
| WO2005065814A1 (en) | 2004-01-07 | 2005-07-21 | Solexa Limited | Modified molecular arrays |
| US7595160B2 (en) | 2004-01-13 | 2009-09-29 | U.S. Genomics, Inc. | Analyte detection using barcoded polymers |
| US7727744B2 (en) | 2004-03-30 | 2010-06-01 | Epicentre Technologies Corporation | Methods for obtaining directionally truncated polypeptides |
| US7608434B2 (en) | 2004-08-04 | 2009-10-27 | Wisconsin Alumni Research Foundation | Mutated Tn5 transposase proteins and the use thereof |
| US7315019B2 (en) | 2004-09-17 | 2008-01-01 | Pacific Biosciences Of California, Inc. | Arrays of optical confinements and uses thereof |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| US20070128610A1 (en) | 2005-12-02 | 2007-06-07 | Buzby Philip R | Sample preparation method and apparatus for nucleic acid sequencing |
| US7537897B2 (en) | 2006-01-23 | 2009-05-26 | Population Genetics Technologies, Ltd. | Molecular counting |
| WO2007107710A1 (en) | 2006-03-17 | 2007-09-27 | Solexa Limited | Isothermal methods for creating clonal single molecule arrays |
| EP3722409A1 (en) | 2006-03-31 | 2020-10-14 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
| WO2008005459A2 (en) | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
| US7754429B2 (en) | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
| US8343746B2 (en) | 2006-10-23 | 2013-01-01 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| WO2008076406A2 (en) | 2006-12-14 | 2008-06-26 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes using large scale fet arrays |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| CN101802223A (zh) | 2007-08-15 | 2010-08-11 | 香港大学 | 用于高通量亚硫酸氢盐dna-测序的方法和组合物及其用途 |
| US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| EP2963709B1 (en) * | 2008-10-24 | 2017-05-24 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| US20110311506A1 (en) | 2009-02-25 | 2011-12-22 | The Johns Hopkins University | Piggybac transposon variants and methods of use |
| US8148515B1 (en) | 2009-06-02 | 2012-04-03 | Biotium, Inc. | Detection using a dye and a dye modifier |
| EP2545183B1 (en) * | 2010-03-10 | 2017-04-19 | Ibis Biosciences, Inc. | Production of single-stranded circular nucleic acid |
| US9029103B2 (en) | 2010-08-27 | 2015-05-12 | Illumina Cambridge Limited | Methods for sequencing polynucleotides |
| EP2632593B1 (en) | 2010-10-27 | 2021-09-29 | Illumina, Inc. | Flow cells for biological or chemical analysis |
| EP2635679B1 (en) | 2010-11-05 | 2017-04-19 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US9074251B2 (en) * | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
| EP3037536B1 (en) * | 2011-01-28 | 2019-11-27 | Illumina, Inc. | Oligonucleotide replacement for di-tagged and directional libraries |
| EP2710172B1 (en) * | 2011-05-20 | 2017-03-29 | Fluidigm Corporation | Nucleic acid encoding reactions |
| US9005935B2 (en) * | 2011-05-23 | 2015-04-14 | Agilent Technologies, Inc. | Methods and compositions for DNA fragmentation and tagging by transposases |
| US20130017978A1 (en) * | 2011-07-11 | 2013-01-17 | Finnzymes Oy | Methods and transposon nucleic acids for generating a dna library |
| NO2694769T3 (OSRAM) * | 2012-03-06 | 2018-03-03 | ||
| US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
| EP3494997B1 (en) | 2012-07-25 | 2019-09-18 | The Broad Institute, Inc. | Inducible dna binding proteins and genome perturbation tools and applications thereof |
| US9644199B2 (en) | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| EP3919617A1 (en) | 2013-03-13 | 2021-12-08 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
| AU2014364180B2 (en) * | 2013-12-09 | 2021-03-04 | Illumina, Inc. | Methods and compositions for targeted nucleic acid sequencing |
| EP3845640A3 (en) | 2014-04-15 | 2021-09-01 | Illumina, Inc. | Modified tranposases for improved insertion sequence bias and increased dna input tolerence |
-
2015
- 2015-06-26 ES ES15734543T patent/ES2713153T3/es active Active
- 2015-06-26 AU AU2015284464A patent/AU2015284464B2/en active Active
- 2015-06-26 CA CA2953791A patent/CA2953791A1/en active Pending
- 2015-06-26 DK DK15734543.0T patent/DK3161152T3/en active
- 2015-06-26 JP JP2016575734A patent/JP6652512B2/ja active Active
- 2015-06-26 SG SG11201610910QA patent/SG11201610910QA/en unknown
- 2015-06-26 CN CN201580036230.6A patent/CN106661561B/zh active Active
- 2015-06-26 EP EP15734543.0A patent/EP3161152B1/en active Active
- 2015-06-26 CN CN202011083287.0A patent/CN112430641A/zh active Pending
- 2015-06-26 US US15/322,432 patent/US10577603B2/en active Active
- 2015-06-26 WO PCT/US2015/038050 patent/WO2016003814A1/en not_active Ceased
-
2020
- 2020-01-07 US US16/736,043 patent/US10968448B2/en active Active
-
2021
- 2021-03-02 US US17/189,916 patent/US11965158B2/en active Active
-
2024
- 2024-03-20 US US18/610,717 patent/US12371690B2/en active Active
-
2025
- 2025-06-24 US US19/247,175 patent/US20250382609A1/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100120098A1 (en) * | 2008-10-24 | 2010-05-13 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| WO2012106546A2 (en) * | 2011-02-02 | 2012-08-09 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
Non-Patent Citations (1)
| Title |
|---|
| LESCHZINER A E ET AL, "Tn552 transposase catalyzes concerted strand transfer in vitro", PNAS, US, (1998-06-01), vol. 95, doi:10.1073/PNAS.95.13.7345, ISSN 0027-8424, pages 7345 - 7350 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP6652512B2 (ja) | 2020-02-26 |
| US20250382609A1 (en) | 2025-12-18 |
| US11965158B2 (en) | 2024-04-23 |
| WO2016003814A1 (en) | 2016-01-07 |
| US20180201925A1 (en) | 2018-07-19 |
| DK3161152T3 (en) | 2019-03-25 |
| JP2017520580A (ja) | 2017-07-27 |
| EP3161152B1 (en) | 2018-12-26 |
| US12371690B2 (en) | 2025-07-29 |
| ES2713153T3 (es) | 2019-05-20 |
| CN106661561A (zh) | 2017-05-10 |
| US20200208144A1 (en) | 2020-07-02 |
| SG11201610910QA (en) | 2017-01-27 |
| CA2953791A1 (en) | 2016-01-07 |
| US10968448B2 (en) | 2021-04-06 |
| EP3161152A1 (en) | 2017-05-03 |
| US20240294904A1 (en) | 2024-09-05 |
| AU2015284464A1 (en) | 2017-02-02 |
| US20210277388A1 (en) | 2021-09-09 |
| US10577603B2 (en) | 2020-03-03 |
| CN112430641A (zh) | 2021-03-02 |
| CN106661561B (zh) | 2020-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12371690B2 (en) | Methods and compositions using one-sided transposition | |
| AU2021257967B2 (en) | Methods and compositions for preparing sequencing libraries | |
| AU2022204365B2 (en) | Preserving genomic connectivity information in fragmented genomic DNA samples | |
| HK40047492A (en) | Methods and compositions using one-sided transposition | |
| HK1235086B (en) | Methods and compositions using one-sided transposition | |
| HK1235086A1 (en) | Methods and compositions using one-sided transposition | |
| HK40036457A (en) | Methods and compositions for preparing sequencing libraries |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |