JP2017520261A5 - - Google Patents
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- Publication number
- JP2017520261A5 JP2017520261A5 JP2017500849A JP2017500849A JP2017520261A5 JP 2017520261 A5 JP2017520261 A5 JP 2017520261A5 JP 2017500849 A JP2017500849 A JP 2017500849A JP 2017500849 A JP2017500849 A JP 2017500849A JP 2017520261 A5 JP2017520261 A5 JP 2017520261A5
- Authority
- JP
- Japan
- Prior art keywords
- cistron
- promoter
- heavy
- ranibizumab
- fragments
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 claims 25
- 102000004169 proteins and genes Human genes 0.000 claims 25
- 238000000034 method Methods 0.000 claims 14
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims 6
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims 6
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims 6
- 239000012634 fragment Substances 0.000 claims 6
- 210000003000 inclusion body Anatomy 0.000 claims 6
- 229960003876 ranibizumab Drugs 0.000 claims 6
- 241000588724 Escherichia coli Species 0.000 claims 5
- 239000002157 polynucleotide Substances 0.000 claims 5
- 102000040430 polynucleotide Human genes 0.000 claims 5
- 108091033319 polynucleotide Proteins 0.000 claims 5
- 230000009977 dual effect Effects 0.000 claims 4
- 210000004027 cell Anatomy 0.000 claims 3
- 238000012258 culturing Methods 0.000 claims 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 claims 2
- 230000003381 solubilizing effect Effects 0.000 claims 2
- 230000001131 transforming effect Effects 0.000 claims 2
- 101100084022 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) lapA gene Proteins 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 101150009573 phoA gene Proteins 0.000 claims 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN2245MU2014 | 2014-07-09 | ||
| IN2245/MUM/2014 | 2014-07-09 | ||
| PCT/IB2015/055189 WO2016005931A1 (en) | 2014-07-09 | 2015-07-09 | Dual cistronic bacterial expression system |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017520261A JP2017520261A (ja) | 2017-07-27 |
| JP2017520261A5 true JP2017520261A5 (enExample) | 2018-08-09 |
| JP6748061B2 JP6748061B2 (ja) | 2020-08-26 |
Family
ID=53773474
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017500849A Active JP6748061B2 (ja) | 2014-07-09 | 2015-07-09 | 二重シストロン細菌発現システム |
Country Status (20)
| Country | Link |
|---|---|
| US (2) | US10329571B2 (enExample) |
| EP (1) | EP3167065B1 (enExample) |
| JP (1) | JP6748061B2 (enExample) |
| CN (1) | CN106661588B (enExample) |
| AU (1) | AU2015287296A1 (enExample) |
| BR (1) | BR112017000389A2 (enExample) |
| CA (1) | CA2954594A1 (enExample) |
| CY (1) | CY1123303T1 (enExample) |
| DK (1) | DK3167065T3 (enExample) |
| ES (1) | ES2808906T3 (enExample) |
| HR (1) | HRP20200996T1 (enExample) |
| HU (1) | HUE050300T2 (enExample) |
| LT (1) | LT3167065T (enExample) |
| MX (1) | MX378585B (enExample) |
| PH (1) | PH12017500047A1 (enExample) |
| PL (1) | PL3167065T3 (enExample) |
| PT (1) | PT3167065T (enExample) |
| RU (1) | RU2732148C2 (enExample) |
| SI (1) | SI3167065T1 (enExample) |
| WO (1) | WO2016005931A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6435770B2 (ja) * | 2014-01-21 | 2018-12-12 | 東ソー株式会社 | シグナルペプチドをコードするポリヌクレオチドおよびそれを用いたタンパク質の製造方法 |
| WO2017021819A1 (en) * | 2015-07-31 | 2017-02-09 | Dr. Reddy’S Laboratories Limited | Process for preparation of protein or peptide |
| KR102607655B1 (ko) | 2017-05-19 | 2023-11-28 | 카운슬 오브 사이언티픽 앤드 인더스트리얼 리서치 | 리폴딩된 재조합 인간화 라니비주맙의 제조 방법 |
| ES2965485T3 (es) * | 2017-08-23 | 2024-04-15 | Novartis Ag | Sistema de expresión de proteínas con múltiples copias génicas |
| CN113631567B (zh) * | 2018-07-06 | 2025-02-07 | 瓦克化学股份公司 | 在发酵方法中释放重组蛋白的细菌菌株 |
| JP7762935B2 (ja) * | 2019-01-31 | 2025-10-31 | Ｓｐｉｂｅｒ株式会社 | 組換え細胞、発現構築物、及び組換えタンパク質の製造方法 |
| KR20210148288A (ko) * | 2019-04-02 | 2021-12-07 | 카운슬 오브 사이언티픽 앤드 인더스트리얼 리서치 | 생체내 리폴딩된 항체 단편의 클로닝 및 발현 |
| CN114774453B (zh) * | 2022-03-14 | 2023-04-21 | 湖北大学 | 一种运动发酵单胞菌基因表达严谨调控系统的构建方法和应用 |
| CN116334118A (zh) * | 2023-02-23 | 2023-06-27 | 四川大学 | 一种原核体系中单启动子控制多个颗粒蛋白亚基组装同源等同体在胞内恒定比例表达并共自组装的方法 |
| CN118995554B (zh) * | 2024-09-09 | 2025-07-04 | 江南大学 | 一种大肠杆菌整合表达体系及其在酶高效表达中的应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7018809B1 (en) | 1991-09-19 | 2006-03-28 | Genentech, Inc. | Expression of functional antibody fragments |
| JP4309656B2 (ja) | 2000-12-14 | 2009-08-05 | ジェネンテック・インコーポレーテッド | 原核生物で生成させた抗体とその用途 |
| CA2454731C (en) | 2001-08-27 | 2010-11-02 | Genentech, Inc. | A system for antibody expression and assembly |
| DK2046382T3 (en) * | 2006-07-10 | 2016-12-12 | Esbatech Alcon Biomed Res Unit | scFv Antibodies which passes through the epithelial and / or endothelial layer |
| RU2413769C1 (ru) * | 2009-12-24 | 2011-03-10 | Учреждение Российской академии наук Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН | РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pET-32a, КОДИРУЮЩАЯ ГЕН ЛИГАНД-СВЯЗЫВАЮЩЕГО ДОМЕНА РЕЦЕПТОРА II ТИПА ТРАНСФОРМИРУЮЩЕГО ФАКТОРА РОСТА-β ЧЕЛОВЕКА (TβRII), ШТАММ БАКТЕРИЙ Escherichia coli - ПРОДУЦЕНТ СЛИТНОГО БЕЛКА ТИОРЕДОКСИН/TβRII И СПОСОБ РЕНАТУРАЦИИ И ОЧИСТКИ ЦЕЛЕВОГО БЕЛКА TβRII |
| GB201002730D0 (en) * | 2010-02-18 | 2010-04-07 | Uni I Oslo | Product |
| US8853380B2 (en) | 2010-06-04 | 2014-10-07 | Lupin Limited | Modified SAK gene for the production of recombinant proteins |
| CA2904146C (en) * | 2013-03-08 | 2023-03-21 | Neuclone Biologics Pty Ltd | A cell expression system |
| CA2911087A1 (en) * | 2013-04-30 | 2014-11-06 | Harish Shandilya | Novel cloning, expression & purification method for the preparation of ranibizumab |
| TW201534726A (zh) * | 2013-07-03 | 2015-09-16 | Halozyme Inc | 熱穩定ｐｈ２０玻尿酸酶變異體及其用途 |
-
2015
- 2015-07-09 ES ES15745247T patent/ES2808906T3/es active Active
- 2015-07-09 RU RU2017102578A patent/RU2732148C2/ru active
- 2015-07-09 LT LTEP15745247.5T patent/LT3167065T/lt unknown
- 2015-07-09 WO PCT/IB2015/055189 patent/WO2016005931A1/en not_active Ceased
- 2015-07-09 DK DK15745247.5T patent/DK3167065T3/da active
- 2015-07-09 PT PT157452475T patent/PT3167065T/pt unknown
- 2015-07-09 CA CA2954594A patent/CA2954594A1/en not_active Abandoned
- 2015-07-09 MX MX2017000332A patent/MX378585B/es unknown
- 2015-07-09 PL PL15745247T patent/PL3167065T3/pl unknown
- 2015-07-09 US US15/324,105 patent/US10329571B2/en not_active Expired - Fee Related
- 2015-07-09 HR HRP20200996TT patent/HRP20200996T1/hr unknown
- 2015-07-09 HU HUE15745247A patent/HUE050300T2/hu unknown
- 2015-07-09 JP JP2017500849A patent/JP6748061B2/ja active Active
- 2015-07-09 EP EP15745247.5A patent/EP3167065B1/en active Active
- 2015-07-09 BR BR112017000389A patent/BR112017000389A2/pt not_active Application Discontinuation
- 2015-07-09 SI SI201531263T patent/SI3167065T1/sl unknown
- 2015-07-09 AU AU2015287296A patent/AU2015287296A1/en not_active Abandoned
- 2015-07-09 CN CN201580037078.3A patent/CN106661588B/zh not_active Expired - Fee Related
-
2017
- 2017-01-06 PH PH12017500047A patent/PH12017500047A1/en unknown
-
2019
- 2019-04-24 US US16/393,338 patent/US11365418B2/en active Active
-
2020
- 2020-06-25 CY CY20201100580T patent/CY1123303T1/el unknown
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