PT3167065T - Sistema de expressão bacteriana dual cistrónica - Google Patents

Sistema de expressão bacteriana dual cistrónica

Info

Publication number
PT3167065T
PT3167065T PT157452475T PT15745247T PT3167065T PT 3167065 T PT3167065 T PT 3167065T PT 157452475 T PT157452475 T PT 157452475T PT 15745247 T PT15745247 T PT 15745247T PT 3167065 T PT3167065 T PT 3167065T
Authority
PT
Portugal
Prior art keywords
expression system
bacterial expression
dual cistronic
cistronic
dual
Prior art date
Application number
PT157452475T
Other languages
English (en)
Portuguese (pt)
Original Assignee
Lupin Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lupin Ltd filed Critical Lupin Ltd
Publication of PT3167065T publication Critical patent/PT3167065T/pt

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/34Cucurbitaceae, e.g. bitter melon, cucumber or watermelon 
    • A01H6/342Citrullus lanatus [watermelon]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/27Erwinia (G)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/605Glucagons
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24Metalloendopeptidases (3.4.24)
    • C12Y304/24029Aureolysin (3.4.24.29)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51Complete heavy chain or Fd fragment, i.e. VH + CH1
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515Complete light chain, i.e. VL + CL
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
PT157452475T 2014-07-09 2015-07-09 Sistema de expressão bacteriana dual cistrónica PT3167065T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN2245MU2014 2014-07-09

Publications (1)

Publication Number Publication Date
PT3167065T true PT3167065T (pt) 2020-07-01

Family

ID=53773474

Family Applications (1)

Application Number Title Priority Date Filing Date
PT157452475T PT3167065T (pt) 2014-07-09 2015-07-09 Sistema de expressão bacteriana dual cistrónica

Country Status (20)

Country Link
US (2) US10329571B2 (enExample)
EP (1) EP3167065B1 (enExample)
JP (1) JP6748061B2 (enExample)
CN (1) CN106661588B (enExample)
AU (1) AU2015287296A1 (enExample)
BR (1) BR112017000389A2 (enExample)
CA (1) CA2954594A1 (enExample)
CY (1) CY1123303T1 (enExample)
DK (1) DK3167065T3 (enExample)
ES (1) ES2808906T3 (enExample)
HR (1) HRP20200996T1 (enExample)
HU (1) HUE050300T2 (enExample)
LT (1) LT3167065T (enExample)
MX (1) MX378585B (enExample)
PH (1) PH12017500047A1 (enExample)
PL (1) PL3167065T3 (enExample)
PT (1) PT3167065T (enExample)
RU (1) RU2732148C2 (enExample)
SI (1) SI3167065T1 (enExample)
WO (1) WO2016005931A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6435770B2 (ja) * 2014-01-21 2018-12-12 東ソー株式会社 シグナルペプチドをコードするポリヌクレオチドおよびそれを用いたタンパク質の製造方法
WO2017021819A1 (en) * 2015-07-31 2017-02-09 Dr. Reddy’S Laboratories Limited Process for preparation of protein or peptide
KR102607655B1 (ko) 2017-05-19 2023-11-28 카운슬 오브 사이언티픽 앤드 인더스트리얼 리서치 리폴딩된 재조합 인간화 라니비주맙의 제조 방법
ES2965485T3 (es) * 2017-08-23 2024-04-15 Novartis Ag Sistema de expresión de proteínas con múltiples copias génicas
CN113631567B (zh) * 2018-07-06 2025-02-07 瓦克化学股份公司 在发酵方法中释放重组蛋白的细菌菌株
JP7762935B2 (ja) * 2019-01-31 2025-10-31 Spiber株式会社 組換え細胞、発現構築物、及び組換えタンパク質の製造方法
KR20210148288A (ko) * 2019-04-02 2021-12-07 카운슬 오브 사이언티픽 앤드 인더스트리얼 리서치 생체내 리폴딩된 항체 단편의 클로닝 및 발현
CN114774453B (zh) * 2022-03-14 2023-04-21 湖北大学 一种运动发酵单胞菌基因表达严谨调控系统的构建方法和应用
CN116334118A (zh) * 2023-02-23 2023-06-27 四川大学 一种原核体系中单启动子控制多个颗粒蛋白亚基组装同源等同体在胞内恒定比例表达并共自组装的方法
CN118995554B (zh) * 2024-09-09 2025-07-04 江南大学 一种大肠杆菌整合表达体系及其在酶高效表达中的应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7018809B1 (en) 1991-09-19 2006-03-28 Genentech, Inc. Expression of functional antibody fragments
JP4309656B2 (ja) 2000-12-14 2009-08-05 ジェネンテック・インコーポレーテッド 原核生物で生成させた抗体とその用途
CA2454731C (en) 2001-08-27 2010-11-02 Genentech, Inc. A system for antibody expression and assembly
DK2046382T3 (en) * 2006-07-10 2016-12-12 Esbatech Alcon Biomed Res Unit scFv Antibodies which passes through the epithelial and / or endothelial layer
RU2413769C1 (ru) * 2009-12-24 2011-03-10 Учреждение Российской академии наук Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pET-32a, КОДИРУЮЩАЯ ГЕН ЛИГАНД-СВЯЗЫВАЮЩЕГО ДОМЕНА РЕЦЕПТОРА II ТИПА ТРАНСФОРМИРУЮЩЕГО ФАКТОРА РОСТА-β ЧЕЛОВЕКА (TβRII), ШТАММ БАКТЕРИЙ Escherichia coli - ПРОДУЦЕНТ СЛИТНОГО БЕЛКА ТИОРЕДОКСИН/TβRII И СПОСОБ РЕНАТУРАЦИИ И ОЧИСТКИ ЦЕЛЕВОГО БЕЛКА TβRII
GB201002730D0 (en) * 2010-02-18 2010-04-07 Uni I Oslo Product
US8853380B2 (en) 2010-06-04 2014-10-07 Lupin Limited Modified SAK gene for the production of recombinant proteins
CA2904146C (en) * 2013-03-08 2023-03-21 Neuclone Biologics Pty Ltd A cell expression system
CA2911087A1 (en) * 2013-04-30 2014-11-06 Harish Shandilya Novel cloning, expression & purification method for the preparation of ranibizumab
TW201534726A (zh) * 2013-07-03 2015-09-16 Halozyme Inc 熱穩定ph20玻尿酸酶變異體及其用途

Also Published As

Publication number Publication date
CY1123303T1 (el) 2021-12-31
EP3167065B1 (en) 2020-03-25
US20190309313A1 (en) 2019-10-10
CN106661588B (zh) 2020-11-06
ES2808906T3 (es) 2021-03-02
CA2954594A1 (en) 2016-01-14
AU2015287296A1 (en) 2017-02-02
WO2016005931A1 (en) 2016-01-14
HRP20200996T1 (hr) 2021-01-08
US11365418B2 (en) 2022-06-21
US20170159060A1 (en) 2017-06-08
BR112017000389A2 (pt) 2018-01-23
US10329571B2 (en) 2019-06-25
DK3167065T3 (da) 2020-07-06
PL3167065T3 (pl) 2020-11-16
CN106661588A (zh) 2017-05-10
EP3167065A1 (en) 2017-05-17
SI3167065T1 (sl) 2020-09-30
HUE050300T2 (hu) 2020-11-30
JP2017520261A (ja) 2017-07-27
LT3167065T (lt) 2020-07-27
RU2732148C2 (ru) 2020-09-11
MX2017000332A (es) 2017-07-28
MX378585B (es) 2025-03-10
PH12017500047A1 (en) 2017-05-22
JP6748061B2 (ja) 2020-08-26

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