JP2017505127A5 - - Google Patents
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- JP2017505127A5 JP2017505127A5 JP2016548101A JP2016548101A JP2017505127A5 JP 2017505127 A5 JP2017505127 A5 JP 2017505127A5 JP 2016548101 A JP2016548101 A JP 2016548101A JP 2016548101 A JP2016548101 A JP 2016548101A JP 2017505127 A5 JP2017505127 A5 JP 2017505127A5
- Authority
- JP
- Japan
- Prior art keywords
- segment
- cell
- locus
- cells
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 210000004027 cell Anatomy 0.000 claims 31
- 241000699666 Mus <mouse, genus> Species 0.000 claims 12
- 108010091086 Recombinases Proteins 0.000 claims 8
- 102000018120 Recombinases Human genes 0.000 claims 8
- 210000003719 b-lymphocyte Anatomy 0.000 claims 7
- 241000699670 Mus sp. Species 0.000 claims 6
- 210000001671 embryonic stem cell Anatomy 0.000 claims 5
- 239000000427 antigen Substances 0.000 claims 4
- 108091007433 antigens Proteins 0.000 claims 4
- 102000036639 antigens Human genes 0.000 claims 4
- 210000002459 blastocyst Anatomy 0.000 claims 4
- 241000124008 Mammalia Species 0.000 claims 3
- 108091033409 CRISPR Proteins 0.000 claims 2
- 108010051219 Cre recombinase Proteins 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 108700029233 VDJ Exons Proteins 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 230000035800 maturation Effects 0.000 claims 2
- 150000007523 nucleic acids Chemical group 0.000 claims 2
- 230000002093 peripheral effect Effects 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 210000000130 stem cell Anatomy 0.000 claims 2
- 230000003844 B-cell-activation Effects 0.000 claims 1
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- 102100032768 Complement receptor type 2 Human genes 0.000 claims 1
- 101000941929 Homo sapiens Complement receptor type 2 Proteins 0.000 claims 1
- 230000003213 activating effect Effects 0.000 claims 1
- 230000004913 activation Effects 0.000 claims 1
- 239000012190 activator Substances 0.000 claims 1
- 230000007547 defect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 210000003297 immature b lymphocyte Anatomy 0.000 claims 1
- 230000003053 immunization Effects 0.000 claims 1
- 230000000415 inactivating effect Effects 0.000 claims 1
- 210000003519 mature b lymphocyte Anatomy 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 238000005215 recombination Methods 0.000 claims 1
- 230000006798 recombination Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461931074P | 2014-01-24 | 2014-01-24 | |
| US61/931,074 | 2014-01-24 | ||
| PCT/US2015/012577 WO2015112790A2 (en) | 2014-01-24 | 2015-01-23 | High-throughput mouse model for optimizing antibody affinities |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017505127A JP2017505127A (ja) | 2017-02-16 |
| JP2017505127A5 true JP2017505127A5 (cg-RX-API-DMAC7.html) | 2018-03-01 |
| JP6479024B2 JP6479024B2 (ja) | 2019-03-06 |
Family
ID=53682124
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016548101A Active JP6479024B2 (ja) | 2014-01-24 | 2015-01-23 | 抗体親和性の最適化のための高スループットマウスモデル |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US10034463B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3096609B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6479024B2 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2015112790A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2597945T3 (da) | 2010-07-26 | 2020-09-21 | Trianni Inc | Transgene dyr og fremgangsmåder til anvendelse deraf |
| US10793829B2 (en) | 2010-07-26 | 2020-10-06 | Trianni, Inc. | Transgenic mammals and methods of use thereof |
| US10662256B2 (en) | 2010-07-26 | 2020-05-26 | Trianni, Inc. | Transgenic mammals and methods of use thereof |
| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| KR20200136508A (ko) | 2013-04-16 | 2020-12-07 | 리제너론 파마슈티칼스 인코포레이티드 | 랫트 게놈의 표적화된 변형 |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| CN111218447B (zh) | 2013-11-07 | 2024-10-11 | 爱迪塔斯医药有限公司 | 使用统治型gRNA的CRISPR相关方法和组合物 |
| JP6174811B2 (ja) | 2013-12-11 | 2017-08-02 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | ゲノムの標的改変のための方法及び組成物 |
| US20150166982A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting pi3k point mutations |
| JP6479024B2 (ja) * | 2014-01-24 | 2019-03-06 | ザ チルドレンズ メディカル センター コーポレーション | 抗体親和性の最適化のための高スループットマウスモデル |
| EP3177718B1 (en) | 2014-07-30 | 2022-03-16 | President and Fellows of Harvard College | Cas9 proteins including ligand-dependent inteins |
| EP3521437B1 (en) | 2014-11-21 | 2025-01-15 | Regeneron Pharmaceuticals, Inc. | Methods for targeted genetic modification using paired guide rnas |
| SG11201803173VA (en) | 2015-10-23 | 2018-05-30 | Harvard College | Nucleobase editors and uses thereof |
| US10813346B2 (en) | 2015-12-03 | 2020-10-27 | Trianni, Inc. | Enhanced immunoglobulin diversity |
| EP3411476B1 (en) | 2016-02-04 | 2024-04-24 | Trianni, Inc. | Enhanced production of immunoglobulins |
| EP3440194A1 (en) * | 2016-04-04 | 2019-02-13 | ETH Zurich | Mammalian cell line for protein production and library generation |
| JP7231935B2 (ja) | 2016-08-03 | 2023-03-08 | プレジデント アンド フェローズ オブ ハーバード カレッジ | アデノシン核酸塩基編集因子およびそれらの使用 |
| CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| WO2018071868A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| CN110637083A (zh) * | 2017-03-20 | 2019-12-31 | 华盛顿大学 | 细胞及其使用和制备方法 |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| CN110809582B (zh) * | 2017-05-01 | 2023-12-22 | 儿童医疗中心有限公司 | 涉及抗pd1抗体试剂的方法以及组合物 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| EP3658573A1 (en) | 2017-07-28 | 2020-06-03 | President and Fellows of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace) |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| AU2018352592C1 (en) | 2017-10-16 | 2025-09-25 | Beam Therapeutics, Inc. | Uses of adenosine base editors |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US20210238312A1 (en) * | 2018-06-13 | 2021-08-05 | The Children's Medical Center Corporation | Methods and compositions relating to high-throughput models for antibody discovery and/or optimization |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| GB2601617B (en) | 2019-03-19 | 2024-02-21 | Broad Inst Inc | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
| WO2022256739A2 (en) * | 2021-06-04 | 2022-12-08 | Nonagen Therapeutics Corporation | Antibody specific for bcl-6 and methods of use |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6130364A (en) | 1995-03-29 | 2000-10-10 | Abgenix, Inc. | Production of antibodies using Cre-mediated site-specific recombination |
| EP2669298A3 (en) * | 2008-05-23 | 2014-02-26 | Ablexis, LLC | Single variable immunoglobulin domain comprising VL-DH-JL |
| US20110314563A1 (en) | 2008-12-18 | 2011-12-22 | Kingdon Craig R | Antibody production |
| IL273982B2 (en) | 2011-08-05 | 2023-03-01 | Regeneron Pharma | Humanized mice possess a universal light chain |
| EP2758535B1 (en) * | 2011-09-19 | 2016-11-09 | Kymab Limited | Antibodies, variable domains&chains tailored for human use |
| KR20250021626A (ko) * | 2011-10-17 | 2025-02-13 | 리제너론 파마슈티칼스 인코포레이티드 | 제한된 면역글로불린 중쇄 마우스 |
| MX364449B (es) * | 2012-06-12 | 2019-04-26 | Regeneron Pharma | Animales no humanos humanizados con loci de cadena pesada de inmunoglobulina restringidos. |
| JP6479024B2 (ja) * | 2014-01-24 | 2019-03-06 | ザ チルドレンズ メディカル センター コーポレーション | 抗体親和性の最適化のための高スループットマウスモデル |
-
2015
- 2015-01-23 JP JP2016548101A patent/JP6479024B2/ja active Active
- 2015-01-23 WO PCT/US2015/012577 patent/WO2015112790A2/en not_active Ceased
- 2015-01-23 EP EP15739843.9A patent/EP3096609B1/en active Active
- 2015-01-23 US US15/112,557 patent/US10034463B2/en active Active
-
2018
- 2018-06-25 US US16/017,221 patent/US11974553B2/en active Active
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