JP2017501148A5 - - Google Patents
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- Publication number
- JP2017501148A5 JP2017501148A5 JP2016538783A JP2016538783A JP2017501148A5 JP 2017501148 A5 JP2017501148 A5 JP 2017501148A5 JP 2016538783 A JP2016538783 A JP 2016538783A JP 2016538783 A JP2016538783 A JP 2016538783A JP 2017501148 A5 JP2017501148 A5 JP 2017501148A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- mixture
- conjugation
- chemical moiety
- reduced
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 17
- 239000000203 mixture Substances 0.000 claims 16
- 235000018102 proteins Nutrition 0.000 claims 11
- 102000004169 proteins and genes Human genes 0.000 claims 11
- 108090000623 proteins and genes Proteins 0.000 claims 11
- 230000021615 conjugation Effects 0.000 claims 7
- 239000000126 substance Substances 0.000 claims 5
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 claims 4
- 239000003638 chemical reducing agent Substances 0.000 claims 4
- 238000006722 reduction reaction Methods 0.000 claims 4
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims 3
- 238000002360 preparation method Methods 0.000 claims 3
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims 2
- XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Chemical compound P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 claims 2
- 238000009295 crossflow filtration Methods 0.000 claims 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims 2
- 102000009027 Albumins Human genes 0.000 claims 1
- 108010088751 Albumins Proteins 0.000 claims 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 claims 1
- 108010024636 Glutathione Proteins 0.000 claims 1
- 102000018997 Growth Hormone Human genes 0.000 claims 1
- 108010051696 Growth Hormone Proteins 0.000 claims 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 1
- 235000018417 cysteine Nutrition 0.000 claims 1
- 229960002433 cysteine Drugs 0.000 claims 1
- 238000011026 diafiltration Methods 0.000 claims 1
- 239000012538 diafiltration buffer Substances 0.000 claims 1
- 229960003180 glutathione Drugs 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 229960003151 mercaptamine Drugs 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 229910000073 phosphorus hydride Inorganic materials 0.000 claims 1
- 150000003568 thioethers Chemical class 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13197150.9 | 2013-12-13 | ||
| EP13197150 | 2013-12-13 | ||
| PCT/EP2014/077777 WO2015086853A1 (en) | 2013-12-13 | 2014-12-15 | Method for thioether conjugation of proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017501148A JP2017501148A (ja) | 2017-01-12 |
| JP2017501148A5 true JP2017501148A5 (cg-RX-API-DMAC7.html) | 2018-01-25 |
| JP6603662B2 JP6603662B2 (ja) | 2019-11-06 |
Family
ID=49765887
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016538783A Active JP6603662B2 (ja) | 2013-12-13 | 2014-12-15 | タンパク質のチオエーテルコンジュゲーション方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US11219690B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3079722B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6603662B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN106068127B (cg-RX-API-DMAC7.html) |
| ES (1) | ES2869309T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2015086853A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10294303B2 (en) | 2015-12-23 | 2019-05-21 | Amgen Inc. | Method of treating or ameliorating metabolic disorders using binding proteins for gastric inhibitory peptide receptor (GIPR) in combination with GLP-1 agonists |
| JOP20190177A1 (ar) | 2017-01-17 | 2019-07-16 | Amgen Inc | طريقة لعلاج أو تحسين اضطرابات أيضية باستخدام مساعدات مستقبل glp-1 مقترنة بمناهضات لمستقبل ببتيد مثبط معوي (gipr) |
| KR20200019122A (ko) | 2017-06-20 | 2020-02-21 | 암젠 인크 | Glp-1 작용제와 조합하여 위 저해 펩타이드 수용체(gipr)에 대한 결합 단백질을 이용하여 대사 장애를 치료하거나 또는 개선시키는 방법 |
| CA3066251A1 (en) | 2017-06-21 | 2018-12-27 | Amgen Inc. | Method of treating or ameliorating metabolic disorders using antagonistic binding proteins for gastric inhibitory peptide receptor (gipr)/glp-1 receptor agonist fusion proteins |
| ES2997332T3 (en) * | 2017-08-04 | 2025-02-17 | Amgen Inc | Method of conjugation of cys-mabs |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2296770A1 (en) | 1997-07-14 | 1999-01-28 | Bolder Biotechnology, Inc. | Derivatives of growth hormone and related proteins |
| US6753165B1 (en) | 1999-01-14 | 2004-06-22 | Bolder Biotechnology, Inc. | Methods for making proteins containing free cysteine residues |
| US7306931B2 (en) | 2000-05-16 | 2007-12-11 | Bolder Biotechnology, Inc. | Method for refolding proteins containing free cysteine residues |
| KR100731826B1 (ko) | 1999-01-14 | 2007-06-22 | 볼더 바이오테크놀로지 인코퍼레이티드 | 유리 시스테인 잔기를 함유하는 단백질의 제조 방법 |
| WO2004101597A2 (en) | 2003-05-13 | 2004-11-25 | Frutarom Ltd. | Methods for the reduction of disulfide bonds |
| CA2552043A1 (en) | 2004-01-21 | 2005-08-04 | Novo Nordisk A/S | Transglutaminase mediated conjugation of peptides |
| EP1893632B1 (en) | 2005-06-17 | 2015-08-12 | Novo Nordisk Health Care AG | Selective reduction and derivatization of engineered factor vii proteins comprising at least one non-native cysteine |
| GB0513852D0 (en) * | 2005-07-06 | 2005-08-10 | Celltech R&D Ltd | Biological products |
| WO2007109475A2 (en) * | 2006-03-16 | 2007-09-27 | Bracco Imaging S.P.A. | Chelation of metals to thiol groups using in situ reduction of disulfide-containing compounds by phosphines |
| US8586532B2 (en) | 2007-08-24 | 2013-11-19 | Novo Nordisk Healthcare A/G | Method for selectively modifying a protein via transglutaminase catalyzed reaction |
| RS59459B1 (sr) | 2010-01-22 | 2019-11-29 | Novo Nordisk Healthcare Ag | Hormoni rasta sa produženom in-vivo efikasnošću |
| JP5980689B2 (ja) | 2010-01-22 | 2016-08-31 | ノヴォ・ノルディスク・ヘルス・ケア・アーゲー | 安定な成長ホルモン化合物 |
| WO2011101261A2 (en) | 2010-02-16 | 2011-08-25 | Novo Nordisk A/S | Purification method |
| WO2012010516A1 (en) | 2010-07-22 | 2012-01-26 | Novo Nordisk Health Care Ag | Growth hormone conjugates |
| EP3808378A1 (en) | 2011-05-27 | 2021-04-21 | Baxalta GmbH | Therapeutic proteins with increased half-life and methods of preparing same |
| CN107163101B (zh) * | 2011-11-02 | 2021-08-17 | 弗·哈夫曼-拉罗切有限公司 | 超载和洗脱层析 |
| CN103087183A (zh) | 2013-01-17 | 2013-05-08 | 南京邮电大学 | 一种光激发蛋白质中二硫键还原得到自由巯基的方法 |
-
2014
- 2014-12-15 CN CN201480068335.5A patent/CN106068127B/zh active Active
- 2014-12-15 ES ES14818938T patent/ES2869309T3/es active Active
- 2014-12-15 EP EP14818938.4A patent/EP3079722B1/en active Active
- 2014-12-15 WO PCT/EP2014/077777 patent/WO2015086853A1/en not_active Ceased
- 2014-12-15 JP JP2016538783A patent/JP6603662B2/ja active Active
- 2014-12-15 US US15/103,482 patent/US11219690B2/en active Active
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