JP2016534727A5 - - Google Patents
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- JP2016534727A5 JP2016534727A5 JP2016526812A JP2016526812A JP2016534727A5 JP 2016534727 A5 JP2016534727 A5 JP 2016534727A5 JP 2016526812 A JP2016526812 A JP 2016526812A JP 2016526812 A JP2016526812 A JP 2016526812A JP 2016534727 A5 JP2016534727 A5 JP 2016534727A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- polynucleotide
- seq
- plant
- optimal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108091033319 polynucleotide Proteins 0.000 claims description 75
- 102000040430 polynucleotide Human genes 0.000 claims description 75
- 239000002157 polynucleotide Substances 0.000 claims description 75
- 241000196324 Embryophyta Species 0.000 claims description 44
- 230000027455 binding Effects 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 19
- 230000009261 transgenic effect Effects 0.000 claims description 19
- 108090000623 proteins and genes Proteins 0.000 claims description 16
- 101710163270 Nuclease Proteins 0.000 claims description 15
- 230000004568 DNA-binding Effects 0.000 claims description 10
- 108091008324 binding proteins Proteins 0.000 claims description 8
- 102000023732 binding proteins Human genes 0.000 claims description 8
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 6
- 239000013612 plasmid Substances 0.000 claims description 6
- 239000011701 zinc Substances 0.000 claims description 6
- 229910052725 zinc Inorganic materials 0.000 claims description 6
- 108091008146 restriction endonucleases Proteins 0.000 claims description 5
- 240000000385 Brassica napus var. napus Species 0.000 claims description 4
- 108091033409 CRISPR Proteins 0.000 claims description 4
- 238000010354 CRISPR gene editing Methods 0.000 claims description 4
- 241001057636 Dracaena deremensis Species 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 4
- 240000002024 Gossypium herbaceum Species 0.000 claims description 4
- 235000004341 Gossypium herbaceum Nutrition 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 4
- 238000010459 TALEN Methods 0.000 claims description 4
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims description 4
- 241000209140 Triticum Species 0.000 claims description 4
- 230000014509 gene expression Effects 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 230000008263 repair mechanism Effects 0.000 claims description 3
- 108091026890 Coding region Proteins 0.000 claims description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
- 230000010354 integration Effects 0.000 claims description 2
- 230000011987 methylation Effects 0.000 claims description 2
- 238000007069 methylation reaction Methods 0.000 claims description 2
- 230000006780 non-homologous end joining Effects 0.000 claims description 2
- 150000007523 nucleic acids Chemical group 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 230000006798 recombination Effects 0.000 claims description 2
- 238000005215 recombination Methods 0.000 claims description 2
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 claims 1
- 230000007246 mechanism Effects 0.000 claims 1
- 239000003550 marker Substances 0.000 description 7
- 230000010076 replication Effects 0.000 description 3
- 108700008625 Reporter Genes Proteins 0.000 description 2
- 108700019146 Transgenes Proteins 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 101100442689 Caenorhabditis elegans hdl-1 gene Proteins 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 101000632261 Homo sapiens Semaphorin-3A Proteins 0.000 description 1
- 102100027974 Semaphorin-3A Human genes 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 101150054900 gus gene Proteins 0.000 description 1
- 101150113864 pat gene Proteins 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 1
- 229960001225 rifampicin Drugs 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361899543P | 2013-11-04 | 2013-11-04 | |
| US61/899,543 | 2013-11-04 | ||
| PCT/US2014/063732 WO2015066637A1 (en) | 2013-11-04 | 2014-11-03 | A universal donor system for gene targeting |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016534727A JP2016534727A (ja) | 2016-11-10 |
| JP2016534727A5 true JP2016534727A5 (enExample) | 2017-11-30 |
| JP6560203B2 JP6560203B2 (ja) | 2019-08-14 |
Family
ID=53005271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016526812A Expired - Fee Related JP6560203B2 (ja) | 2013-11-04 | 2014-11-03 | 遺伝子ターゲッティングのためのユニバーサルドナー系 |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US10077449B2 (enExample) |
| EP (1) | EP3066192B1 (enExample) |
| JP (1) | JP6560203B2 (enExample) |
| KR (1) | KR102248730B1 (enExample) |
| CN (1) | CN105683357B (enExample) |
| AR (1) | AR098283A1 (enExample) |
| AU (1) | AU2014341928B2 (enExample) |
| BR (1) | BR102014027448A8 (enExample) |
| CA (1) | CA2926534A1 (enExample) |
| IL (1) | IL245313B (enExample) |
| RU (1) | RU2687369C2 (enExample) |
| TW (1) | TWI669395B (enExample) |
| WO (1) | WO2015066637A1 (enExample) |
| ZA (1) | ZA201601929B (enExample) |
Families Citing this family (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112013024337A2 (pt) | 2011-03-23 | 2017-09-26 | Du Pont | locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão |
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| KR20230054509A (ko) | 2013-11-07 | 2023-04-24 | 에디타스 메디신, 인코포레이티드 | 지배적인 gRNA를 이용하는 CRISPR-관련 방법 및 조성물 |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| WO2016040030A1 (en) | 2014-09-12 | 2016-03-17 | E. I. Du Pont De Nemours And Company | Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use |
| CN107250148B (zh) | 2014-12-03 | 2021-04-16 | 安捷伦科技有限公司 | 具有化学修饰的指导rna |
| KR102046450B1 (ko) | 2015-01-27 | 2019-11-19 | 인스티튜트 오브 제네틱스 앤드 디벨롭멘털 바이오롤지, 차이니즈 아카데미 오브 사이언시스 | 일시적인 유전자 발현을 통해 완전한 식물에서 부위-특이적인 변형을 수행하는 방법 |
| ES2884838T3 (es) | 2015-04-06 | 2021-12-13 | Univ Leland Stanford Junior | ARN guía químicamente modificados para la regulación génica mediada por CRISPR/CAS |
| EP3095870A1 (en) | 2015-05-19 | 2016-11-23 | Kws Saat Se | Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom |
| CA2994883A1 (en) * | 2015-08-14 | 2017-02-23 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Scnces | Method for obtaining glyphosate-resistant rice by site-directed nucleotide substitution |
| WO2017040967A1 (en) * | 2015-09-04 | 2017-03-09 | Octavo Systems Llc | Improved system using system in package components |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| US11306308B2 (en) | 2015-11-13 | 2022-04-19 | Massachusetts Institute Of Technology | High-throughput CRISPR-based library screening |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| CN106591293A (zh) * | 2016-12-28 | 2017-04-26 | 贵州省草业研究所 | 基于酶切连接从未知基因组中分离已知序列侧翼序列的方法 |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| CN107545153B (zh) * | 2017-10-25 | 2021-06-11 | 桂林电子科技大学 | 一种基于卷积神经网络的核小体分类预测方法 |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| GB2611929B (en) | 2018-10-16 | 2023-11-22 | Blueallele Corp | Methods for targeted insertion of DNA in genes |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001306T5 (de) | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
| CN114317588B (zh) * | 2020-09-29 | 2023-06-27 | 中国农业科学院生物技术研究所 | 一种作物高温响应型基因组精准甲基化调控回路 |
| US11884915B2 (en) | 2021-09-10 | 2024-01-30 | Agilent Technologies, Inc. | Guide RNAs with chemical modification for prime editing |
| WO2023049728A1 (en) * | 2021-09-21 | 2023-03-30 | Pairwise Plants Services, Inc. | Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same |
| CN114369677A (zh) * | 2022-01-04 | 2022-04-19 | 江汉大学 | 一种检测小麦转基因成分和转基因品系的引物组合、试剂盒、检测方法及应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ526507A (en) * | 2001-01-26 | 2005-07-29 | Commw Scient Ind Res Org | Methods and means for producing efficient silencing construct using recombinational cloning |
| US20030232410A1 (en) * | 2002-03-21 | 2003-12-18 | Monika Liljedahl | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
| WO2008021207A2 (en) * | 2006-08-11 | 2008-02-21 | Dow Agrosciences Llc | Zinc finger nuclease-mediated homologous recombination |
| CA2689345C (en) * | 2007-06-05 | 2017-07-11 | Bayer Bioscience N.V. | Methods and means for exact replacement of target dna in eukaryotic organisms |
| CN106995819B (zh) * | 2008-07-01 | 2020-10-20 | 孟山都技术公司 | 用于调控靶基因表达的重组dna构建体和方法 |
| SI2370575T1 (en) * | 2008-12-17 | 2018-07-31 | Dow Agrosciences, Llc | Targeted integration in the Zp15 locus |
| DE112010001772T5 (de) * | 2009-03-20 | 2012-10-18 | Basf Plant Science Company Gmbh | Nematodenresistente transgene pflanzen |
| JP2013517774A (ja) | 2010-01-22 | 2013-05-20 | ダウ アグロサイエンシィズ エルエルシー | 遺伝子改変生物における導入遺伝子の切除 |
| HUE033500T2 (en) * | 2010-01-22 | 2017-12-28 | Dow Agrosciences Llc | Engineered landing pads for gene targeting in plants |
| EP2580336B1 (en) | 2010-06-09 | 2017-05-10 | Bayer CropScience NV | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
| EP2729567B1 (en) | 2011-07-08 | 2016-10-05 | Cellectis | Method for increasing the efficiency of double-strand break-induced mutagenssis |
| AU2014315335B2 (en) * | 2013-09-04 | 2017-08-24 | Corteva Agriscience Llc | Rapid targeting analysis in crops for determining donor insertion |
-
2014
- 2014-11-03 AU AU2014341928A patent/AU2014341928B2/en not_active Ceased
- 2014-11-03 KR KR1020167014602A patent/KR102248730B1/ko not_active Expired - Fee Related
- 2014-11-03 RU RU2016122038A patent/RU2687369C2/ru active
- 2014-11-03 AR ARP140104120A patent/AR098283A1/es unknown
- 2014-11-03 CN CN201480060417.5A patent/CN105683357B/zh not_active Expired - Fee Related
- 2014-11-03 US US14/531,710 patent/US10077449B2/en not_active Expired - Fee Related
- 2014-11-03 TW TW103138081A patent/TWI669395B/zh not_active IP Right Cessation
- 2014-11-03 JP JP2016526812A patent/JP6560203B2/ja not_active Expired - Fee Related
- 2014-11-03 WO PCT/US2014/063732 patent/WO2015066637A1/en not_active Ceased
- 2014-11-03 BR BR102014027448A patent/BR102014027448A8/pt not_active Application Discontinuation
- 2014-11-03 EP EP14858498.0A patent/EP3066192B1/en not_active Not-in-force
- 2014-11-03 CA CA2926534A patent/CA2926534A1/en not_active Abandoned
-
2016
- 2016-03-18 ZA ZA2016/01929A patent/ZA201601929B/en unknown
- 2016-04-25 IL IL245313A patent/IL245313B/en active IP Right Grant
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