JP2016521541A5 - - Google Patents
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- JP2016521541A5 JP2016521541A5 JP2016514171A JP2016514171A JP2016521541A5 JP 2016521541 A5 JP2016521541 A5 JP 2016521541A5 JP 2016514171 A JP2016514171 A JP 2016514171A JP 2016514171 A JP2016514171 A JP 2016514171A JP 2016521541 A5 JP2016521541 A5 JP 2016521541A5
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- Prior art keywords
- cell
- cells
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- mrna
- oct4
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- 210000004027 cell Anatomy 0.000 claims description 79
- 238000000034 method Methods 0.000 claims description 66
- 108020004999 messenger RNA Proteins 0.000 claims description 24
- 230000008672 reprogramming Effects 0.000 claims description 14
- 210000001082 somatic cell Anatomy 0.000 claims description 12
- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 claims description 11
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 6
- 101000687905 Homo sapiens Transcription factor SOX-2 Proteins 0.000 claims description 6
- 102100024270 Transcription factor SOX-2 Human genes 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims description 4
- 101001139134 Homo sapiens Krueppel-like factor 4 Proteins 0.000 claims description 4
- 101001030211 Homo sapiens Myc proto-oncogene protein Proteins 0.000 claims description 4
- 101000984042 Homo sapiens Protein lin-28 homolog A Proteins 0.000 claims description 4
- 102100020677 Krueppel-like factor 4 Human genes 0.000 claims description 4
- 102100038895 Myc proto-oncogene protein Human genes 0.000 claims description 4
- 101710126211 POU domain, class 5, transcription factor 1 Proteins 0.000 claims description 4
- 102100025460 Protein lin-28 homolog A Human genes 0.000 claims description 4
- 101001023030 Toxoplasma gondii Myosin-D Proteins 0.000 claims description 4
- 210000004962 mammalian cell Anatomy 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 3
- 230000004927 fusion Effects 0.000 claims description 3
- 210000002894 multi-fate stem cell Anatomy 0.000 claims description 3
- 238000013518 transcription Methods 0.000 claims description 3
- 230000035897 transcription Effects 0.000 claims description 3
- 241000283690 Bos taurus Species 0.000 claims description 2
- 241000282465 Canis Species 0.000 claims description 2
- 101100239628 Danio rerio myca gene Proteins 0.000 claims description 2
- 101100411708 Danio rerio rarga gene Proteins 0.000 claims description 2
- 241000283073 Equus caballus Species 0.000 claims description 2
- 108010068250 Herpes Simplex Virus Protein Vmw65 Proteins 0.000 claims description 2
- 108700021430 Kruppel-Like Factor 4 Proteins 0.000 claims description 2
- 241000282567 Macaca fascicularis Species 0.000 claims description 2
- 241000282560 Macaca mulatta Species 0.000 claims description 2
- 102100035423 POU domain, class 5, transcription factor 1 Human genes 0.000 claims description 2
- 241001504519 Papio ursinus Species 0.000 claims description 2
- 241001494479 Pecora Species 0.000 claims description 2
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 claims description 2
- 108020004459 Small interfering RNA Proteins 0.000 claims description 2
- 230000032459 dedifferentiation Effects 0.000 claims description 2
- 208000035475 disorder Diseases 0.000 claims description 2
- 210000005260 human cell Anatomy 0.000 claims description 2
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 2
- 230000001939 inductive effect Effects 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 claims description 2
- 101150111214 lin-28 gene Proteins 0.000 claims description 2
- 108091070501 miRNA Proteins 0.000 claims description 2
- 239000002679 microRNA Substances 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 208000024891 symptom Diseases 0.000 claims description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 5
- 108091023045 Untranslated Region Proteins 0.000 description 4
- 239000013612 plasmid Substances 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 108020003589 5' Untranslated Regions Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101000989934 Mus musculus Hemoglobin subunit alpha Proteins 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021121453A JP2021166553A (ja) | 2013-05-13 | 2021-07-26 | 合成メッセンジャーｒｎａによるヒト人工多能性幹細胞のフィーダーフリー誘導 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/893,166 US10155929B2 (en) | 2012-05-13 | 2013-05-13 | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
| PCT/US2014/040362 WO2014190361A2 (en) | 2013-05-13 | 2014-05-30 | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019134561A Division JP2019170405A (ja) | 2019-07-22 | 2019-07-22 | 合成メッセンジャーｒｎａによるヒト人工多能性幹細胞のフィーダーフリー誘導 |
| JP2021121453A Division JP2021166553A (ja) | 2013-05-13 | 2021-07-26 | 合成メッセンジャーｒｎａによるヒト人工多能性幹細胞のフィーダーフリー誘導 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016521541A JP2016521541A (ja) | 2016-07-25 |
| JP2016521541A5 true JP2016521541A5 (https=) | 2017-05-25 |
| JP6971574B2 JP6971574B2 (ja) | 2021-11-24 |
Family
ID=51939013
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016514171A Active JP6971574B2 (ja) | 2013-05-13 | 2014-05-30 | 合成メッセンジャーｒｎａによるヒト人工多能性幹細胞のフィーダーフリー誘導 |
| JP2021121453A Withdrawn JP2021166553A (ja) | 2013-05-13 | 2021-07-26 | 合成メッセンジャーｒｎａによるヒト人工多能性幹細胞のフィーダーフリー誘導 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021121453A Withdrawn JP2021166553A (ja) | 2013-05-13 | 2021-07-26 | 合成メッセンジャーｒｎａによるヒト人工多能性幹細胞のフィーダーフリー誘導 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US10155929B2 (https=) |
| EP (2) | EP2997130A4 (https=) |
| JP (2) | JP6971574B2 (https=) |
| CN (1) | CN105555949A (https=) |
| HK (2) | HK1224335A1 (https=) |
| MX (2) | MX2015015840A (https=) |
| SG (2) | SG11201509368PA (https=) |
| WO (1) | WO2014190361A2 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8497124B2 (en) | 2011-12-05 | 2013-07-30 | Factor Bioscience Inc. | Methods and products for reprogramming cells to a less differentiated state |
| AU2012347919B2 (en) | 2011-12-05 | 2017-02-02 | Factor Bioscience Inc. | Methods and products for transfecting cells |
| US10155929B2 (en) * | 2012-05-13 | 2018-12-18 | Allele Biotechnology & Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
| US10119150B2 (en) * | 2012-05-13 | 2018-11-06 | Allele Biotechnology & Pharmaceuticals, Inc. | Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
| EP3561054B1 (en) * | 2012-05-13 | 2023-07-05 | Allele Biotechnology And Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
| JP2015522257A (ja) * | 2012-06-13 | 2015-08-06 | ステムジェント， インコーポレイテッドＳｔｅｍｇｅｎｔ， Ｉｎｃ． | 多能性幹細胞を調製する方法 |
| KR102121086B1 (ko) | 2012-11-01 | 2020-06-09 | 팩터 바이오사이언스 인크. | 세포에서 단백질을 발현시키는 방법들과 생성물들 |
| EP3099801B1 (en) | 2014-01-31 | 2020-03-18 | Factor Bioscience Inc. | Synthetic rna for use in the treatment of dystrophic epidermolysis bullosa |
| CA2950582C (en) * | 2014-05-30 | 2020-09-22 | Allele Biotechnology And Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
| CA2976376A1 (en) | 2015-02-13 | 2016-08-18 | Factor Bioscience Inc. | Nucleic acid products and methods of administration thereof |
| WO2019241569A1 (en) * | 2018-06-16 | 2019-12-19 | Luigi Warren | Reprogramming cells with synthetic messenger rna |
| CN105567642B (zh) * | 2016-02-01 | 2019-07-12 | 中国科学院生物物理研究所 | 一种着色性干皮病人多能干细胞的制备方法 |
| IL308824A (en) | 2016-08-17 | 2024-01-01 | Factor Bioscience Inc | Nucleic acid products and methods of administration thereof |
| US12338462B2 (en) | 2016-11-16 | 2025-06-24 | Allele Biotechnology And Pharmaceuticals, Inc. | Induction of pancreatic beta cells by stem cell differentiation with RNA |
| KR102586507B1 (ko) | 2016-11-16 | 2023-10-11 | 알렐 바이오테크놀로지 앤 파마슈티칼스, 인크. | Ｒｎａ를 사용한 줄기 세포 분화에 의한 간세포의 유도 |
| EP3564362A4 (en) * | 2016-12-28 | 2020-07-08 | Kyoto Prefectural Public University Corporation | SKELETAL MUSCLE CELLS AND THEIR INDUCTION METHOD |
| CN107142244B (zh) * | 2017-07-03 | 2019-12-20 | 广州润虹医药科技股份有限公司 | 培养基及其用途与诱导多能干细胞向间充质干细胞转化的方法 |
| US12104171B2 (en) | 2017-07-13 | 2024-10-01 | Allele Biotechnology And Pharmaceuticals, Inc. | Induction of neural progenitor cells, oligodendrocyte progenitor cells, and oligodendrocytes by stem cell differentiation using landmark transcription factors |
| KR20200131301A (ko) * | 2018-03-13 | 2020-11-23 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | 세포 노화의 역전을 위한 일시적 세포 재프로그래밍 |
| EP3969571A4 (en) | 2019-05-14 | 2023-05-24 | Aleph Farms Ltd. | PLURIPOTENTE CELL AGGREGATES AND THEIR USE |
| CA3145425A1 (en) | 2019-07-03 | 2021-01-07 | Factor Bioscience Inc. | Cationic lipids and uses thereof |
| US10501404B1 (en) | 2019-07-30 | 2019-12-10 | Factor Bioscience Inc. | Cationic lipids and transfection methods |
| AU2021300378A1 (en) * | 2020-07-01 | 2023-01-19 | Elevatebio Technologies, Inc. | Compositions and methods for cellular reprogramming using circular RNA |
| JP2022104813A (ja) | 2020-12-29 | 2022-07-11 | アイ ピース，インコーポレイテッド | リプログラミング因子を導入された細胞の培養方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5260432A (en) | 1989-06-22 | 1993-11-09 | Sloan-Kettering Institute For Cancer Research | Human gamma retinoic acid receptor DNA |
| JP2008307007A (ja) * | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| SG160248A1 (en) * | 2008-09-18 | 2010-04-29 | Agency Science Tech & Res | Use of novel monoclonal antibodies targeting human embryonic stem cells to characterize and kill induced pluripotent stem cells |
| EP2881461A1 (en) | 2008-11-21 | 2015-06-10 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Reprogramming cells toward a pluripotent state |
| WO2010108126A2 (en) | 2009-03-19 | 2010-09-23 | Fate Therapeutics, Inc. | Reprogramming compositions and methods of using the same |
| CA2770412C (en) * | 2009-08-07 | 2018-09-11 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| CN102190731B (zh) * | 2010-03-09 | 2016-01-27 | 中国科学院上海生命科学研究院 | 用人工转录因子诱导产生多能干细胞 |
| WO2011140397A2 (en) * | 2010-05-05 | 2011-11-10 | The Regents Of The University Of California Office Of The President | Stem cell defined media for xeno-free and feeder free conditions and uses thereof |
| WO2011142832A2 (en) | 2010-05-13 | 2011-11-17 | Whitehead Institute For Biomedical Research | Stem cells derived under low oxygen conditions |
| US9580689B2 (en) | 2010-07-22 | 2017-02-28 | Regents Of The University Of Minnesota | Induced pluripotent stem cells |
| AU2012246822B2 (en) * | 2011-04-22 | 2017-05-25 | Biomarin Technologies B.V. | New compounds for treating, delaying and/or preventing a human genetic disorder such as myotonic dystrophy type 1 (DM1) |
| US8497124B2 (en) | 2011-12-05 | 2013-07-30 | Factor Bioscience Inc. | Methods and products for reprogramming cells to a less differentiated state |
| AU2013222188A1 (en) | 2012-02-22 | 2014-09-11 | Amgen Inc. | Autologous mammalian models derived from induced pluripotent stem cells and related methods |
| EP3561054B1 (en) | 2012-05-13 | 2023-07-05 | Allele Biotechnology And Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
| US10155929B2 (en) | 2012-05-13 | 2018-12-18 | Allele Biotechnology & Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
-
2013
- 2013-05-13 US US13/893,166 patent/US10155929B2/en active Active
-
2014
- 2014-05-30 JP JP2016514171A patent/JP6971574B2/ja active Active
- 2014-05-30 SG SG11201509368PA patent/SG11201509368PA/en unknown
- 2014-05-30 MX MX2015015840A patent/MX2015015840A/es unknown
- 2014-05-30 EP EP14801588.6A patent/EP2997130A4/en not_active Withdrawn
- 2014-05-30 WO PCT/US2014/040362 patent/WO2014190361A2/en not_active Ceased
- 2014-05-30 EP EP21154853.2A patent/EP3904504A1/en active Pending
- 2014-05-30 HK HK16112614.3A patent/HK1224335A1/zh unknown
- 2014-05-30 SG SG10202006695TA patent/SG10202006695TA/en unknown
- 2014-05-30 HK HK16110689.7A patent/HK1222675A1/zh unknown
- 2014-05-30 CN CN201480033794.XA patent/CN105555949A/zh active Pending
-
2015
- 2015-11-13 MX MX2021005715A patent/MX2021005715A/es unknown
-
2021
- 2021-07-26 JP JP2021121453A patent/JP2021166553A/ja not_active Withdrawn
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