SG11201509368PA - Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna - Google Patents

Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna

Info

Publication number
SG11201509368PA
SG11201509368PA SG11201509368PA SG11201509368PA SG11201509368PA SG 11201509368P A SG11201509368P A SG 11201509368PA SG 11201509368P A SG11201509368P A SG 11201509368PA SG 11201509368P A SG11201509368P A SG 11201509368PA SG 11201509368P A SG11201509368P A SG 11201509368PA
Authority
SG
Singapore
Prior art keywords
feeder
human
stem cells
pluripotent stem
messenger rna
Prior art date
Application number
SG11201509368PA
Other languages
English (en)
Inventor
Jiwu Wang
Original Assignee
Allele Biotechnology & Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Allele Biotechnology & Pharmaceuticals Inc filed Critical Allele Biotechnology & Pharmaceuticals Inc
Publication of SG11201509368PA publication Critical patent/SG11201509368PA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/603—Oct-3/4
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/605—Nanog
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/608—Lin28
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Neurosurgery (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Neurology (AREA)
  • Transplantation (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Hospice & Palliative Care (AREA)
  • Dermatology (AREA)
  • Psychiatry (AREA)
  • Psychology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
SG11201509368PA 2013-05-13 2014-05-30 Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna SG11201509368PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/893,166 US10155929B2 (en) 2012-05-13 2013-05-13 Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
PCT/US2014/040362 WO2014190361A2 (en) 2013-05-13 2014-05-30 Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna

Publications (1)

Publication Number Publication Date
SG11201509368PA true SG11201509368PA (en) 2015-12-30

Family

ID=51939013

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201509368PA SG11201509368PA (en) 2013-05-13 2014-05-30 Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna
SG10202006695TA SG10202006695TA (en) 2013-05-13 2014-05-30 Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10202006695TA SG10202006695TA (en) 2013-05-13 2014-05-30 Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna

Country Status (8)

Country Link
US (1) US10155929B2 (https=)
EP (2) EP2997130A4 (https=)
JP (2) JP6971574B2 (https=)
CN (1) CN105555949A (https=)
HK (2) HK1224335A1 (https=)
MX (2) MX2015015840A (https=)
SG (2) SG11201509368PA (https=)
WO (1) WO2014190361A2 (https=)

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US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
AU2012347919B2 (en) 2011-12-05 2017-02-02 Factor Bioscience Inc. Methods and products for transfecting cells
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
US10119150B2 (en) * 2012-05-13 2018-11-06 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
EP3561054B1 (en) * 2012-05-13 2023-07-05 Allele Biotechnology And Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna
JP2015522257A (ja) * 2012-06-13 2015-08-06 ステムジェント， インコーポレイテッドＳｔｅｍｇｅｎｔ， Ｉｎｃ． 多能性幹細胞を調製する方法
KR102121086B1 (ko) 2012-11-01 2020-06-09 팩터 바이오사이언스 인크. 세포에서 단백질을 발현시키는 방법들과 생성물들
EP3099801B1 (en) 2014-01-31 2020-03-18 Factor Bioscience Inc. Synthetic rna for use in the treatment of dystrophic epidermolysis bullosa
CA2950582C (en) * 2014-05-30 2020-09-22 Allele Biotechnology And Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna
CA2976376A1 (en) 2015-02-13 2016-08-18 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
WO2019241569A1 (en) * 2018-06-16 2019-12-19 Luigi Warren Reprogramming cells with synthetic messenger rna
CN105567642B (zh) * 2016-02-01 2019-07-12 中国科学院生物物理研究所 一种着色性干皮病人多能干细胞的制备方法
IL308824A (en) 2016-08-17 2024-01-01 Factor Bioscience Inc Nucleic acid products and methods of administration thereof
US12338462B2 (en) 2016-11-16 2025-06-24 Allele Biotechnology And Pharmaceuticals, Inc. Induction of pancreatic beta cells by stem cell differentiation with RNA
KR102586507B1 (ko) 2016-11-16 2023-10-11 알렐 바이오테크놀로지 앤 파마슈티칼스, 인크. Ｒｎａ를 사용한 줄기 세포 분화에 의한 간세포의 유도
EP3564362A4 (en) * 2016-12-28 2020-07-08 Kyoto Prefectural Public University Corporation SKELETAL MUSCLE CELLS AND THEIR INDUCTION METHOD
CN107142244B (zh) * 2017-07-03 2019-12-20 广州润虹医药科技股份有限公司 培养基及其用途与诱导多能干细胞向间充质干细胞转化的方法
US12104171B2 (en) 2017-07-13 2024-10-01 Allele Biotechnology And Pharmaceuticals, Inc. Induction of neural progenitor cells, oligodendrocyte progenitor cells, and oligodendrocytes by stem cell differentiation using landmark transcription factors
KR20200131301A (ko) * 2018-03-13 2020-11-23 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 세포 노화의 역전을 위한 일시적 세포 재프로그래밍
EP3969571A4 (en) 2019-05-14 2023-05-24 Aleph Farms Ltd. PLURIPOTENTE CELL AGGREGATES AND THEIR USE
CA3145425A1 (en) 2019-07-03 2021-01-07 Factor Bioscience Inc. Cationic lipids and uses thereof
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
AU2021300378A1 (en) * 2020-07-01 2023-01-19 Elevatebio Technologies, Inc. Compositions and methods for cellular reprogramming using circular RNA
JP2022104813A (ja) 2020-12-29 2022-07-11 アイ ピース，インコーポレイテッド リプログラミング因子を導入された細胞の培養方法

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EP3561054B1 (en) 2012-05-13 2023-07-05 Allele Biotechnology And Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna
US10155929B2 (en) 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA

Also Published As

Publication number Publication date
WO2014190361A2 (en) 2014-11-27
US10155929B2 (en) 2018-12-18
HK1224335A1 (zh) 2017-08-18
MX2015015840A (es) 2016-08-11
EP2997130A4 (en) 2017-03-01
EP2997130A2 (en) 2016-03-23
HK1222675A1 (zh) 2017-07-07
JP2016521541A (ja) 2016-07-25
EP3904504A1 (en) 2021-11-03
WO2014190361A3 (en) 2015-07-30
JP2021166553A (ja) 2021-10-21
JP6971574B2 (ja) 2021-11-24
SG10202006695TA (en) 2020-08-28
US20130302295A1 (en) 2013-11-14
CN105555949A (zh) 2016-05-04
MX2021005715A (es) 2021-07-21

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