JP2016512044A5 - - Google Patents
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- JP2016512044A5 JP2016512044A5 JP2016502597A JP2016502597A JP2016512044A5 JP 2016512044 A5 JP2016512044 A5 JP 2016512044A5 JP 2016502597 A JP2016502597 A JP 2016502597A JP 2016502597 A JP2016502597 A JP 2016502597A JP 2016512044 A5 JP2016512044 A5 JP 2016512044A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- antigen
- specific
- optionally
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 210000003719 b-lymphocyte Anatomy 0.000 claims 74
- 239000000427 antigen Substances 0.000 claims 71
- 102000036639 antigens Human genes 0.000 claims 71
- 108091007433 antigens Proteins 0.000 claims 71
- 210000004027 cell Anatomy 0.000 claims 25
- 238000000034 method Methods 0.000 claims 19
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 16
- 239000006228 supernatant Substances 0.000 claims 14
- 238000010186 staining Methods 0.000 claims 11
- 239000011324 bead Substances 0.000 claims 8
- 229960002685 biotin Drugs 0.000 claims 8
- 235000020958 biotin Nutrition 0.000 claims 8
- 239000011616 biotin Substances 0.000 claims 8
- 238000012258 culturing Methods 0.000 claims 8
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 claims 8
- 230000005764 inhibitory process Effects 0.000 claims 7
- 108010090804 Streptavidin Proteins 0.000 claims 6
- 230000006698 induction Effects 0.000 claims 6
- 238000002965 ELISA Methods 0.000 claims 5
- 238000000684 flow cytometry Methods 0.000 claims 5
- 239000011159 matrix material Substances 0.000 claims 5
- 229920001184 polypeptide Polymers 0.000 claims 5
- 108090000765 processed proteins & peptides Proteins 0.000 claims 5
- 102000004196 processed proteins & peptides Human genes 0.000 claims 5
- 238000012216 screening Methods 0.000 claims 5
- 241000894007 species Species 0.000 claims 5
- 241000283973 Oryctolagus cuniculus Species 0.000 claims 4
- 238000004519 manufacturing process Methods 0.000 claims 4
- 238000010187 selection method Methods 0.000 claims 4
- 239000007787 solid Substances 0.000 claims 4
- 210000001744 T-lymphocyte Anatomy 0.000 claims 3
- 239000011248 coating agent Substances 0.000 claims 3
- 238000000576 coating method Methods 0.000 claims 3
- 230000008014 freezing Effects 0.000 claims 3
- 238000007710 freezing Methods 0.000 claims 3
- 230000035755 proliferation Effects 0.000 claims 3
- 238000003757 reverse transcription PCR Methods 0.000 claims 3
- 238000005406 washing Methods 0.000 claims 3
- 101001098868 Homo sapiens Proprotein convertase subtilisin/kexin type 9 Proteins 0.000 claims 2
- 102100038955 Proprotein convertase subtilisin/kexin type 9 Human genes 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 210000004369 blood Anatomy 0.000 claims 2
- 239000008280 blood Substances 0.000 claims 2
- 210000001185 bone marrow Anatomy 0.000 claims 2
- 239000003636 conditioned culture medium Substances 0.000 claims 2
- 230000005714 functional activity Effects 0.000 claims 2
- 230000003053 immunization Effects 0.000 claims 2
- 238000002649 immunization Methods 0.000 claims 2
- 210000001165 lymph node Anatomy 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 claims 2
- 230000000704 physical effect Effects 0.000 claims 2
- 238000011176 pooling Methods 0.000 claims 2
- 238000003127 radioimmunoassay Methods 0.000 claims 2
- 239000012429 reaction media Substances 0.000 claims 2
- 210000000952 spleen Anatomy 0.000 claims 2
- 238000003860 storage Methods 0.000 claims 2
- 108090001008 Avidin Proteins 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- 108090000932 Calcitonin Gene-Related Peptide Proteins 0.000 claims 1
- 102100025588 Calcitonin gene-related peptide 1 Human genes 0.000 claims 1
- 241000700199 Cavia porcellus Species 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 101001051093 Homo sapiens Low-density lipoprotein receptor Proteins 0.000 claims 1
- 108090001005 Interleukin-6 Proteins 0.000 claims 1
- 241000235058 Komagataella pastoris Species 0.000 claims 1
- 102100024640 Low-density lipoprotein receptor Human genes 0.000 claims 1
- 241000699666 Mus <mouse, genus> Species 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 claims 1
- 102000015336 Nerve Growth Factor Human genes 0.000 claims 1
- 108010025020 Nerve Growth Factor Proteins 0.000 claims 1
- 241000235648 Pichia Species 0.000 claims 1
- 241000700159 Rattus Species 0.000 claims 1
- 238000001261 affinity purification Methods 0.000 claims 1
- 230000001270 agonistic effect Effects 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 230000003042 antagnostic effect Effects 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 230000008236 biological pathway Effects 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000003491 cAMP production Effects 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 238000010367 cloning Methods 0.000 claims 1
- 230000009089 cytolysis Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 238000003306 harvesting Methods 0.000 claims 1
- 238000003018 immunoassay Methods 0.000 claims 1
- 238000001114 immunoprecipitation Methods 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 238000002826 magnetic-activated cell sorting Methods 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000002858 neurotransmitter agent Substances 0.000 claims 1
- 230000003472 neutralizing effect Effects 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 claims 1
- 230000035899 viability Effects 0.000 claims 1
- 238000001262 western blot Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361791755P | 2013-03-15 | 2013-03-15 | |
| US61/791,755 | 2013-03-15 | ||
| PCT/US2014/030994 WO2014146074A2 (en) | 2013-03-15 | 2014-03-18 | Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016512044A JP2016512044A (ja) | 2016-04-25 |
| JP2016512044A5 true JP2016512044A5 (https=) | 2017-04-20 |
| JP6466397B2 JP6466397B2 (ja) | 2019-02-06 |
Family
ID=51538609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016502597A Expired - Fee Related JP6466397B2 (ja) | 2013-03-15 | 2014-03-18 | 抗原特異的ｂ細胞の同定及び単離ならびに所望の抗原に対する抗体作製のプロトコール |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20160033504A1 (https=) |
| EP (1) | EP2972330A4 (https=) |
| JP (1) | JP6466397B2 (https=) |
| KR (1) | KR20150140679A (https=) |
| AU (1) | AU2014232225B2 (https=) |
| CA (1) | CA2907570A1 (https=) |
| IL (1) | IL241433B (https=) |
| WO (1) | WO2014146074A2 (https=) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9255154B2 (en) | 2012-05-08 | 2016-02-09 | Alderbio Holdings, Llc | Anti-PCSK9 antibodies and use thereof |
| GB201316644D0 (en) | 2013-09-19 | 2013-11-06 | Kymab Ltd | Expression vector production & High-Throughput cell screening |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| AU2015230933B2 (en) | 2014-03-21 | 2020-08-13 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| EP4691556A3 (en) | 2014-12-19 | 2026-04-29 | H. Lundbeck A/S | Humanized anti-acth antibodies and use thereof |
| WO2016149137A1 (en) * | 2015-03-18 | 2016-09-22 | Epitomics, Inc. | High throughput monoclonal antibody generation by b cell panning and proliferation |
| US20190056398A1 (en) * | 2016-02-26 | 2019-02-21 | Sri International | Identification and isolation of antibodies from white blood cells |
| KR102444717B1 (ko) | 2016-04-15 | 2022-09-16 | 하. 룬드벡 아크티에셀스카브 | 인간화 항-pacap 항체 및 그의 용도 |
| CN109952314A (zh) | 2016-09-23 | 2019-06-28 | 泰瓦制药国际有限公司 | 治疗难治性偏头痛 |
| US11793833B2 (en) | 2016-12-02 | 2023-10-24 | Juno Therapeutics, Inc. | Engineered B cells and related compositions and methods |
| ES2799175T3 (es) * | 2017-01-20 | 2020-12-15 | Shenzhen New Ind Biomedical Engineering Co Ltd | Método y kit para preparar un par de anticuerpos y uso del kit, y sistema para preparar un par de anticuerpos |
| US11125757B2 (en) * | 2017-05-26 | 2021-09-21 | Emory University | Methods of culturing and characterizing antibody secreting cells |
| CN107058235B (zh) * | 2017-06-05 | 2020-08-11 | 深圳大学 | 一种b细胞筛选方法及其在单克隆抗体制备中的应用 |
| WO2020242856A1 (en) * | 2019-05-31 | 2020-12-03 | The Penn State Research Foundation | Specific selection of immune cells using versatile display scaffolds |
| CN115298323A (zh) * | 2020-01-17 | 2022-11-04 | 嘉普科德基因组学公司 | 靶向测序方法 |
| CN114441752B (zh) * | 2020-11-03 | 2024-11-26 | 中国科学院苏州纳米技术与纳米仿生研究所 | 一种筛选方法 |
| CA3210091A1 (en) * | 2021-02-05 | 2022-08-11 | Amgen Inc. | Non-terminal antibody discovery methods and single cell assays |
| CN115166241B (zh) * | 2022-08-22 | 2023-03-24 | 广东忠信生物科技有限公司 | 一种同时筛选记忆b细胞和浆细胞的高效筛选技术及应用 |
| WO2025240335A1 (en) | 2024-05-13 | 2025-11-20 | Regeneron Pharmaceuticals, Inc. | Fgfr3 binding molecules and methods of use thereof |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990000625A1 (en) * | 1988-07-14 | 1990-01-25 | Monoclonetics International, Inc. | Pre-screening for depletion and enrichment of specific b-cells |
| CA2090126C (en) | 1990-08-02 | 2002-10-22 | John W. Schrader | Methods for the production of proteins with a desired function |
| DK0488470T3 (da) * | 1990-11-26 | 1997-12-22 | Akzo Nobel Nv | Fremgangsmåde til fremstilling af antistoffer |
| US5213960A (en) * | 1992-03-09 | 1993-05-25 | Tanox Biosystems, Inc. | Methods for selecting low frequency antigen-specific single B lymphocytes |
| US5256542A (en) * | 1992-03-09 | 1993-10-26 | Tanox Biosystems, Inc. | Selecting low frequency antigen-specific single B lymphocytes with correction for background noise |
| US20050287607A1 (en) * | 2004-01-07 | 2005-12-29 | Diamond Betty A | Methods and compositions for identification of antigen-specific B cell populations |
| US20060051348A1 (en) | 2004-09-09 | 2006-03-09 | Jorn Gorlach | Method of producing a plurality of isolated antibodies to a plurality of cognate antigens |
| CN101048426A (zh) * | 2004-10-22 | 2007-10-03 | 金克克国际有限公司 | 分离人抗体 |
| EP1951337B1 (en) * | 2005-10-17 | 2018-08-01 | Gambro Lundia AB | Extracorporeal blood cleaning |
| EP2021463B1 (en) * | 2006-05-19 | 2016-11-23 | Alder Biopharmaceuticals, Inc. | Culture method for obtaining a clonal population of antigen-specific b cells |
| WO2008073166A2 (en) * | 2006-08-23 | 2008-06-19 | Xcellerex, Inc. | Multi-variant cell indication technique |
| DK2121920T3 (da) * | 2007-03-01 | 2011-11-21 | Symphogen As | Fremgangsmåde til kloning af sammenhørende antistoffer |
| US20090203037A1 (en) * | 2007-12-27 | 2009-08-13 | Abbott Laboratories | Anti-T. Cruzi Antibodies and Methods of Use |
| SI2400298T1 (sl) * | 2010-05-28 | 2013-11-29 | F. Hoffmann-La Roche Ag | Postopek kultivacije posamezne B-celice in izdelava specifičnih protiteles |
| BR112013022052A2 (pt) * | 2011-03-01 | 2016-11-22 | Novo Nordisk As | ligantes antagonísticos de dr3 |
| ES2617488T3 (es) * | 2011-11-23 | 2017-06-19 | F. Hoffmann-La Roche Ag | Células de mamífero que expresan CD40L y su uso |
| US20150110802A1 (en) * | 2012-02-29 | 2015-04-23 | Medlmmune, Llc | Antibody production methods |
-
2014
- 2014-03-18 US US14/776,945 patent/US20160033504A1/en not_active Abandoned
- 2014-03-18 EP EP14764593.1A patent/EP2972330A4/en not_active Withdrawn
- 2014-03-18 JP JP2016502597A patent/JP6466397B2/ja not_active Expired - Fee Related
- 2014-03-18 KR KR1020157028403A patent/KR20150140679A/ko not_active Abandoned
- 2014-03-18 US US14/217,594 patent/US20140287952A1/en not_active Abandoned
- 2014-03-18 AU AU2014232225A patent/AU2014232225B2/en not_active Ceased
- 2014-03-18 WO PCT/US2014/030994 patent/WO2014146074A2/en not_active Ceased
- 2014-03-18 CA CA2907570A patent/CA2907570A1/en not_active Abandoned
-
2015
- 2015-09-10 IL IL241433A patent/IL241433B/en active IP Right Grant
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