CA2907570A1 - Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens - Google Patents

Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens

Info

Publication number
CA2907570A1
CA2907570A1 CA2907570A CA2907570A CA2907570A1 CA 2907570 A1 CA2907570 A1 CA 2907570A1 CA 2907570 A CA2907570 A CA 2907570A CA 2907570 A CA2907570 A CA 2907570A CA 2907570 A1 CA2907570 A1 CA 2907570A1
Authority
CA
Canada
Prior art keywords
cells
antigen
specific
cell
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2907570A
Other languages
English (en)
French (fr)
Inventor
Daniel S. Allison
Benjamin H. Dutzar
Leon F. Garcia-Martinez
Katie ANDERSON
Ethan W. Ojala
John A. Latham
Jens Billgren
Anne Elisabeth Carvalho Jensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
H Lundbeck AS
Original Assignee
Alder Biopharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alder Biopharmaceuticals Inc filed Critical Alder Biopharmaceuticals Inc
Publication of CA2907570A1 publication Critical patent/CA2907570A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/5052—Cells of the immune system involving B-cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
CA2907570A 2013-03-15 2014-03-18 Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens Abandoned CA2907570A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361791755P 2013-03-15 2013-03-15
US61/791,755 2013-03-15
PCT/US2014/030994 WO2014146074A2 (en) 2013-03-15 2014-03-18 Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens

Publications (1)

Publication Number Publication Date
CA2907570A1 true CA2907570A1 (en) 2014-09-18

Family

ID=51538609

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2907570A Abandoned CA2907570A1 (en) 2013-03-15 2014-03-18 Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens

Country Status (8)

Country Link
US (2) US20160033504A1 (https=)
EP (1) EP2972330A4 (https=)
JP (1) JP6466397B2 (https=)
KR (1) KR20150140679A (https=)
AU (1) AU2014232225B2 (https=)
CA (1) CA2907570A1 (https=)
IL (1) IL241433B (https=)
WO (1) WO2014146074A2 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9255154B2 (en) 2012-05-08 2016-02-09 Alderbio Holdings, Llc Anti-PCSK9 antibodies and use thereof
GB201316644D0 (en) 2013-09-19 2013-11-06 Kymab Ltd Expression vector production & High-Throughput cell screening
US10556945B2 (en) 2014-03-21 2020-02-11 Teva Pharmaceuticals International Gmbh Antagonist antibodies directed against calcitonin gene-related peptide and methods using same
AU2015230933B2 (en) 2014-03-21 2020-08-13 Teva Pharmaceuticals International Gmbh Antagonist antibodies directed against calcitonin gene-related peptide and methods using same
EP4691556A3 (en) 2014-12-19 2026-04-29 H. Lundbeck A/S Humanized anti-acth antibodies and use thereof
WO2016149137A1 (en) * 2015-03-18 2016-09-22 Epitomics, Inc. High throughput monoclonal antibody generation by b cell panning and proliferation
US20190056398A1 (en) * 2016-02-26 2019-02-21 Sri International Identification and isolation of antibodies from white blood cells
KR102444717B1 (ko) 2016-04-15 2022-09-16 하. 룬드벡 아크티에셀스카브 인간화 항-pacap 항체 및 그의 용도
CN109952314A (zh) 2016-09-23 2019-06-28 泰瓦制药国际有限公司 治疗难治性偏头痛
US11793833B2 (en) 2016-12-02 2023-10-24 Juno Therapeutics, Inc. Engineered B cells and related compositions and methods
ES2799175T3 (es) * 2017-01-20 2020-12-15 Shenzhen New Ind Biomedical Engineering Co Ltd Método y kit para preparar un par de anticuerpos y uso del kit, y sistema para preparar un par de anticuerpos
US11125757B2 (en) * 2017-05-26 2021-09-21 Emory University Methods of culturing and characterizing antibody secreting cells
CN107058235B (zh) * 2017-06-05 2020-08-11 深圳大学 一种b细胞筛选方法及其在单克隆抗体制备中的应用
WO2020242856A1 (en) * 2019-05-31 2020-12-03 The Penn State Research Foundation Specific selection of immune cells using versatile display scaffolds
CN115298323A (zh) * 2020-01-17 2022-11-04 嘉普科德基因组学公司 靶向测序方法
CN114441752B (zh) * 2020-11-03 2024-11-26 中国科学院苏州纳米技术与纳米仿生研究所 一种筛选方法
CA3210091A1 (en) * 2021-02-05 2022-08-11 Amgen Inc. Non-terminal antibody discovery methods and single cell assays
CN115166241B (zh) * 2022-08-22 2023-03-24 广东忠信生物科技有限公司 一种同时筛选记忆b细胞和浆细胞的高效筛选技术及应用
WO2025240335A1 (en) 2024-05-13 2025-11-20 Regeneron Pharmaceuticals, Inc. Fgfr3 binding molecules and methods of use thereof

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990000625A1 (en) * 1988-07-14 1990-01-25 Monoclonetics International, Inc. Pre-screening for depletion and enrichment of specific b-cells
CA2090126C (en) 1990-08-02 2002-10-22 John W. Schrader Methods for the production of proteins with a desired function
DK0488470T3 (da) * 1990-11-26 1997-12-22 Akzo Nobel Nv Fremgangsmåde til fremstilling af antistoffer
US5213960A (en) * 1992-03-09 1993-05-25 Tanox Biosystems, Inc. Methods for selecting low frequency antigen-specific single B lymphocytes
US5256542A (en) * 1992-03-09 1993-10-26 Tanox Biosystems, Inc. Selecting low frequency antigen-specific single B lymphocytes with correction for background noise
US20050287607A1 (en) * 2004-01-07 2005-12-29 Diamond Betty A Methods and compositions for identification of antigen-specific B cell populations
US20060051348A1 (en) 2004-09-09 2006-03-09 Jorn Gorlach Method of producing a plurality of isolated antibodies to a plurality of cognate antigens
CN101048426A (zh) * 2004-10-22 2007-10-03 金克克国际有限公司 分离人抗体
EP1951337B1 (en) * 2005-10-17 2018-08-01 Gambro Lundia AB Extracorporeal blood cleaning
EP2021463B1 (en) * 2006-05-19 2016-11-23 Alder Biopharmaceuticals, Inc. Culture method for obtaining a clonal population of antigen-specific b cells
WO2008073166A2 (en) * 2006-08-23 2008-06-19 Xcellerex, Inc. Multi-variant cell indication technique
DK2121920T3 (da) * 2007-03-01 2011-11-21 Symphogen As Fremgangsmåde til kloning af sammenhørende antistoffer
US20090203037A1 (en) * 2007-12-27 2009-08-13 Abbott Laboratories Anti-T. Cruzi Antibodies and Methods of Use
SI2400298T1 (sl) * 2010-05-28 2013-11-29 F. Hoffmann-La Roche Ag Postopek kultivacije posamezne B-celice in izdelava specifičnih protiteles
BR112013022052A2 (pt) * 2011-03-01 2016-11-22 Novo Nordisk As ligantes antagonísticos de dr3
ES2617488T3 (es) * 2011-11-23 2017-06-19 F. Hoffmann-La Roche Ag Células de mamífero que expresan CD40L y su uso
US20150110802A1 (en) * 2012-02-29 2015-04-23 Medlmmune, Llc Antibody production methods

Also Published As

Publication number Publication date
AU2014232225A1 (en) 2015-09-03
EP2972330A4 (en) 2016-10-26
EP2972330A2 (en) 2016-01-20
WO2014146074A2 (en) 2014-09-18
WO2014146074A3 (en) 2014-11-06
AU2014232225B2 (en) 2020-03-19
KR20150140679A (ko) 2015-12-16
IL241433A0 (en) 2015-11-30
US20140287952A1 (en) 2014-09-25
JP2016512044A (ja) 2016-04-25
US20160033504A1 (en) 2016-02-04
JP6466397B2 (ja) 2019-02-06
IL241433B (en) 2019-12-31

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20190315

FZDE Discontinued

Effective date: 20220601