AU2014232225B2 - Protocol for identifying and isolating antigen-specific B cells and producing antibodies to desired antigens - Google Patents

Protocol for identifying and isolating antigen-specific B cells and producing antibodies to desired antigens Download PDF

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Publication number
AU2014232225B2
AU2014232225B2 AU2014232225A AU2014232225A AU2014232225B2 AU 2014232225 B2 AU2014232225 B2 AU 2014232225B2 AU 2014232225 A AU2014232225 A AU 2014232225A AU 2014232225 A AU2014232225 A AU 2014232225A AU 2014232225 B2 AU2014232225 B2 AU 2014232225B2
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Australia
Prior art keywords
cells
antigen
specific
cell
antibody
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AU2014232225A
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AU2014232225A1 (en
Inventor
Daniel S. Allison
Katie ANDERSON
Jens Billgren
Benjamin H. Dutzar
Leon F. Garcia-Martinez
Anne Elisabeth Carvalho Jensen
John A. Latham
Ethan W. Ojala
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H Lundbeck AS
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H Lundbeck AS
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Assigned to H. LUNDBECK A/S. reassignment H. LUNDBECK A/S. Request for Assignment Assignors: LUNDBECK SEATTLE BIOPHARMACEUTICALS, INC
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/5052—Cells of the immune system involving B-cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
AU2014232225A 2013-03-15 2014-03-18 Protocol for identifying and isolating antigen-specific B cells and producing antibodies to desired antigens Ceased AU2014232225B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361791755P 2013-03-15 2013-03-15
US61/791,755 2013-03-15
PCT/US2014/030994 WO2014146074A2 (en) 2013-03-15 2014-03-18 Protocol for identifying and isolating antigen-specific b cells and producing antibodies to desired antigens

Publications (2)

Publication Number Publication Date
AU2014232225A1 AU2014232225A1 (en) 2015-09-03
AU2014232225B2 true AU2014232225B2 (en) 2020-03-19

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Family Applications (1)

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AU2014232225A Ceased AU2014232225B2 (en) 2013-03-15 2014-03-18 Protocol for identifying and isolating antigen-specific B cells and producing antibodies to desired antigens

Country Status (8)

Country Link
US (2) US20160033504A1 (https=)
EP (1) EP2972330A4 (https=)
JP (1) JP6466397B2 (https=)
KR (1) KR20150140679A (https=)
AU (1) AU2014232225B2 (https=)
CA (1) CA2907570A1 (https=)
IL (1) IL241433B (https=)
WO (1) WO2014146074A2 (https=)

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US9255154B2 (en) 2012-05-08 2016-02-09 Alderbio Holdings, Llc Anti-PCSK9 antibodies and use thereof
GB201316644D0 (en) 2013-09-19 2013-11-06 Kymab Ltd Expression vector production & High-Throughput cell screening
US10556945B2 (en) 2014-03-21 2020-02-11 Teva Pharmaceuticals International Gmbh Antagonist antibodies directed against calcitonin gene-related peptide and methods using same
AU2015230933B2 (en) 2014-03-21 2020-08-13 Teva Pharmaceuticals International Gmbh Antagonist antibodies directed against calcitonin gene-related peptide and methods using same
EP4691556A3 (en) 2014-12-19 2026-04-29 H. Lundbeck A/S Humanized anti-acth antibodies and use thereof
WO2016149137A1 (en) * 2015-03-18 2016-09-22 Epitomics, Inc. High throughput monoclonal antibody generation by b cell panning and proliferation
US20190056398A1 (en) * 2016-02-26 2019-02-21 Sri International Identification and isolation of antibodies from white blood cells
KR102444717B1 (ko) 2016-04-15 2022-09-16 하. 룬드벡 아크티에셀스카브 인간화 항-pacap 항체 및 그의 용도
CN109952314A (zh) 2016-09-23 2019-06-28 泰瓦制药国际有限公司 治疗难治性偏头痛
US11793833B2 (en) 2016-12-02 2023-10-24 Juno Therapeutics, Inc. Engineered B cells and related compositions and methods
ES2799175T3 (es) * 2017-01-20 2020-12-15 Shenzhen New Ind Biomedical Engineering Co Ltd Método y kit para preparar un par de anticuerpos y uso del kit, y sistema para preparar un par de anticuerpos
US11125757B2 (en) * 2017-05-26 2021-09-21 Emory University Methods of culturing and characterizing antibody secreting cells
CN107058235B (zh) * 2017-06-05 2020-08-11 深圳大学 一种b细胞筛选方法及其在单克隆抗体制备中的应用
WO2020242856A1 (en) * 2019-05-31 2020-12-03 The Penn State Research Foundation Specific selection of immune cells using versatile display scaffolds
CN115298323A (zh) * 2020-01-17 2022-11-04 嘉普科德基因组学公司 靶向测序方法
CN114441752B (zh) * 2020-11-03 2024-11-26 中国科学院苏州纳米技术与纳米仿生研究所 一种筛选方法
CA3210091A1 (en) * 2021-02-05 2022-08-11 Amgen Inc. Non-terminal antibody discovery methods and single cell assays
CN115166241B (zh) * 2022-08-22 2023-03-24 广东忠信生物科技有限公司 一种同时筛选记忆b细胞和浆细胞的高效筛选技术及应用
WO2025240335A1 (en) 2024-05-13 2025-11-20 Regeneron Pharmaceuticals, Inc. Fgfr3 binding molecules and methods of use thereof

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US20120141982A1 (en) * 2006-05-19 2012-06-07 Anne Elisabeth Carvalho Jensen Culture Method for Obtaining a Clonal Population of Antigen-Specific B Cells

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DK0488470T3 (da) * 1990-11-26 1997-12-22 Akzo Nobel Nv Fremgangsmåde til fremstilling af antistoffer
US5213960A (en) * 1992-03-09 1993-05-25 Tanox Biosystems, Inc. Methods for selecting low frequency antigen-specific single B lymphocytes
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US20060051348A1 (en) 2004-09-09 2006-03-09 Jorn Gorlach Method of producing a plurality of isolated antibodies to a plurality of cognate antigens
CN101048426A (zh) * 2004-10-22 2007-10-03 金克克国际有限公司 分离人抗体
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Also Published As

Publication number Publication date
AU2014232225A1 (en) 2015-09-03
EP2972330A4 (en) 2016-10-26
EP2972330A2 (en) 2016-01-20
WO2014146074A2 (en) 2014-09-18
WO2014146074A3 (en) 2014-11-06
KR20150140679A (ko) 2015-12-16
IL241433A0 (en) 2015-11-30
US20140287952A1 (en) 2014-09-25
CA2907570A1 (en) 2014-09-18
JP2016512044A (ja) 2016-04-25
US20160033504A1 (en) 2016-02-04
JP6466397B2 (ja) 2019-02-06
IL241433B (en) 2019-12-31

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MK14 Patent ceased section 143(a) (annual fees not paid) or expired