JP2016509835A5 - - Google Patents
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- Publication number
- JP2016509835A5 JP2016509835A5 JP2015558979A JP2015558979A JP2016509835A5 JP 2016509835 A5 JP2016509835 A5 JP 2016509835A5 JP 2015558979 A JP2015558979 A JP 2015558979A JP 2015558979 A JP2015558979 A JP 2015558979A JP 2016509835 A5 JP2016509835 A5 JP 2016509835A5
- Authority
- JP
- Japan
- Prior art keywords
- luciferin
- luciferase
- cell
- composition
- fluoroluciferin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 claims 25
- 210000004027 cell Anatomy 0.000 claims 19
- 108060001084 Luciferase Proteins 0.000 claims 17
- 239000005089 Luciferase Substances 0.000 claims 17
- 238000000034 method Methods 0.000 claims 16
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims 14
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 6
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 claims 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims 4
- 239000002518 antifoaming agent Substances 0.000 claims 4
- 210000003527 eukaryotic cell Anatomy 0.000 claims 4
- 238000003556 assay Methods 0.000 claims 3
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims 3
- 235000011180 diphosphates Nutrition 0.000 claims 3
- VICYBMUVWHJEFT-UHFFFAOYSA-N dodecyltrimethylammonium ion Chemical compound CCCCCCCCCCCC[N+](C)(C)C VICYBMUVWHJEFT-UHFFFAOYSA-N 0.000 claims 3
- 238000004020 luminiscence type Methods 0.000 claims 3
- 229910000160 potassium phosphate Inorganic materials 0.000 claims 3
- 235000011009 potassium phosphates Nutrition 0.000 claims 3
- 235000013024 sodium fluoride Nutrition 0.000 claims 3
- 239000011775 sodium fluoride Substances 0.000 claims 3
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 claims 2
- FCKYPQBAHLOOJQ-UHFFFAOYSA-N Cyclohexane-1,2-diaminetetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)C1CCCCC1N(CC(O)=O)CC(O)=O FCKYPQBAHLOOJQ-UHFFFAOYSA-N 0.000 claims 2
- 239000007987 MES buffer Substances 0.000 claims 2
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- 239000007979 citrate buffer Substances 0.000 claims 2
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 claims 2
- 239000005516 coenzyme A Substances 0.000 claims 2
- 229940093530 coenzyme a Drugs 0.000 claims 2
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 claims 2
- 239000003599 detergent Substances 0.000 claims 2
- 229910001425 magnesium ion Inorganic materials 0.000 claims 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims 2
- 210000001236 prokaryotic cell Anatomy 0.000 claims 2
- 229940048084 pyrophosphate Drugs 0.000 claims 2
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 claims 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims 1
- FCKYPQBAHLOOJQ-UWVGGRQHSA-N 2-[[(1s,2s)-2-[bis(carboxymethyl)amino]cyclohexyl]-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)[C@H]1CCCC[C@@H]1N(CC(O)=O)CC(O)=O FCKYPQBAHLOOJQ-UWVGGRQHSA-N 0.000 claims 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 claims 1
- 108091026890 Coding region Proteins 0.000 claims 1
- 241000254173 Coleoptera Species 0.000 claims 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims 1
- 239000007995 HEPES buffer Substances 0.000 claims 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 claims 1
- 239000013592 cell lysate Substances 0.000 claims 1
- 239000002738 chelating agent Substances 0.000 claims 1
- 239000003638 chemical reducing agent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 claims 1
- 230000002934 lysing effect Effects 0.000 claims 1
- 229910052751 metal Inorganic materials 0.000 claims 1
- 239000002184 metal Substances 0.000 claims 1
- 229940124272 protein stabilizer Drugs 0.000 claims 1
- 229940048086 sodium pyrophosphate Drugs 0.000 claims 1
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 claims 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 claims 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361767875P | 2013-02-22 | 2013-02-22 | |
| US61/767,875 | 2013-02-22 | ||
| US201361783726P | 2013-03-14 | 2013-03-14 | |
| US61/783,726 | 2013-03-14 | ||
| PCT/US2014/017562 WO2014130760A1 (en) | 2013-02-22 | 2014-02-21 | Stabilized formulation for luminescent detection of luciferase and nucleoside phosphates |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019033815A Division JP7128134B2 (ja) | 2013-02-22 | 2019-02-27 | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
| JP2020093067A Division JP2020156493A (ja) | 2013-02-22 | 2020-05-28 | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016509835A JP2016509835A (ja) | 2016-04-04 |
| JP2016509835A5 true JP2016509835A5 (enExample) | 2017-03-30 |
Family
ID=50241539
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015558979A Pending JP2016509835A (ja) | 2013-02-22 | 2014-02-21 | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
| JP2019033815A Active JP7128134B2 (ja) | 2013-02-22 | 2019-02-27 | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
| JP2020093067A Pending JP2020156493A (ja) | 2013-02-22 | 2020-05-28 | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
| JP2021086133A Active JP7223057B2 (ja) | 2013-02-22 | 2021-05-21 | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019033815A Active JP7128134B2 (ja) | 2013-02-22 | 2019-02-27 | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
| JP2020093067A Pending JP2020156493A (ja) | 2013-02-22 | 2020-05-28 | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
| JP2021086133A Active JP7223057B2 (ja) | 2013-02-22 | 2021-05-21 | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US9487814B2 (enExample) |
| EP (3) | EP2959010B1 (enExample) |
| JP (4) | JP2016509835A (enExample) |
| CN (3) | CN117701675A (enExample) |
| WO (1) | WO2014130760A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006130551A2 (en) * | 2005-05-31 | 2006-12-07 | Promega Corporation | Luminogenic and fluorogenic compounds and methods to detect molecules or conditions |
| JP2016509835A (ja) | 2013-02-22 | 2016-04-04 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
| WO2019028272A1 (en) | 2017-08-04 | 2019-02-07 | Promega Corporation | COMPOSITIONS AND METHODS FOR STABILIZING LUCIFERIN BENZOTHIAZOLE ANALOGS |
| JP7448993B2 (ja) * | 2019-09-26 | 2024-03-13 | ウィタヤシリメティ インスティチュート オブ サイエンス アンド テクノロジー | ルシフェリン誘導体及びその合成方法 |
| CN112063687B (zh) * | 2020-09-02 | 2021-06-11 | 创芯国际生物科技(广州)有限公司 | 一种3d类器官的atp检测试剂及其制备方法和应用 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8745893B2 (en) | 2011-08-10 | 2014-06-10 | Gavrieli Brands LLC | Split-sole footwear |
| SE428379B (sv) * | 1978-05-31 | 1983-06-27 | Lkb Produkter Ab | Bioluminiscens bestemning av atp och reagens herfor |
| DE2828658C3 (de) * | 1978-06-29 | 1981-10-22 | Lkb-Produkter Ab, Stockholm | Verfahren zur photometrischen Bestimmung der Untereinheit B der Creatinkinase und Reagens hierfür |
| GB9501170D0 (en) | 1994-03-23 | 1995-03-08 | Secr Defence | Luciferases |
| GB9417593D0 (en) | 1994-09-01 | 1994-10-19 | Secr Defence | Luciferase labelling method |
| JP4342608B2 (ja) | 1995-01-20 | 2009-10-14 | スリーエム イノベイティブ プロパティーズ カンパニー | 突然変異体ルシフェラーゼ |
| GB9707486D0 (en) | 1997-04-11 | 1997-05-28 | Secr Defence | Enzyme assays |
| JP4537573B2 (ja) | 1997-09-19 | 2010-09-01 | プロメガ・コーポレイシヨン | 熱安定ルシフェラーゼと生産方法 |
| US6602677B1 (en) * | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
| DE19842901A1 (de) | 1998-09-18 | 2000-03-23 | Lembert Nicolas | Bioluminometrisches Verfahren zum zerstörungsfreien Messen von Zellaktivierungen |
| GB9823468D0 (en) | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
| US7083911B2 (en) | 2001-02-16 | 2006-08-01 | Promega Corporation | Method for detection of ATP |
| EP2218790A3 (en) | 2002-09-06 | 2010-08-25 | Promega Corporation | Kits for detecting transferase enzymatic activity |
| JP4485470B2 (ja) | 2002-12-23 | 2010-06-23 | プロメガ コーポレイション | ルシフェラーゼ酵素活性を保護するための方法及びキット |
| EP1935986B1 (en) | 2005-05-31 | 2014-04-16 | Promega Corporation | Luminogenic and fluorogenic compounds and methods to detect molecules or conditions |
| US7530909B2 (en) | 2005-06-27 | 2009-05-12 | Sop Services, Inc. | Basketball return net mounting system |
| US20090075309A1 (en) | 2007-09-06 | 2009-03-19 | Gambhir Sanjiv S | Bisdeoxycoelenterazine derivatives, methods of use, and BRET2 systems |
| EP2870257B1 (en) | 2012-07-06 | 2017-02-22 | 3M Innovative Properties Company | Apparatus and methods for detecting atp in a liquid sample |
| JP2016509835A (ja) | 2013-02-22 | 2016-04-04 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | ルシフェラーゼ及びヌクレオシドホスフェートの発光検出のための安定化された配合物 |
-
2014
- 2014-02-21 JP JP2015558979A patent/JP2016509835A/ja active Pending
- 2014-02-21 CN CN202311707362.XA patent/CN117701675A/zh active Pending
- 2014-02-21 CN CN201911407470.9A patent/CN111004835B/zh active Active
- 2014-02-21 US US14/186,065 patent/US9487814B2/en active Active
- 2014-02-21 EP EP14709480.9A patent/EP2959010B1/en active Active
- 2014-02-21 EP EP20166813.4A patent/EP3693471B1/en active Active
- 2014-02-21 CN CN201480010140.5A patent/CN105121653B/zh active Active
- 2014-02-21 EP EP23213374.4A patent/EP4306649A3/en active Pending
- 2014-02-21 WO PCT/US2014/017562 patent/WO2014130760A1/en not_active Ceased
-
2016
- 2016-07-20 US US15/215,307 patent/US10501774B2/en active Active
-
2019
- 2019-02-27 JP JP2019033815A patent/JP7128134B2/ja active Active
- 2019-11-01 US US16/672,090 patent/US11214822B2/en active Active
-
2020
- 2020-05-28 JP JP2020093067A patent/JP2020156493A/ja active Pending
-
2021
- 2021-05-21 JP JP2021086133A patent/JP7223057B2/ja active Active
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