JP2016135158A5 - - Google Patents
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- Publication number
- JP2016135158A5 JP2016135158A5 JP2016093169A JP2016093169A JP2016135158A5 JP 2016135158 A5 JP2016135158 A5 JP 2016135158A5 JP 2016093169 A JP2016093169 A JP 2016093169A JP 2016093169 A JP2016093169 A JP 2016093169A JP 2016135158 A5 JP2016135158 A5 JP 2016135158A5
- Authority
- JP
- Japan
- Prior art keywords
- homologous recombination
- nucleic acid
- sequence
- acid molecule
- stranded oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 16
- 230000006801 homologous recombination Effects 0.000 claims 11
- 238000002744 homologous recombination Methods 0.000 claims 11
- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 108091034117 Oligonucleotide Proteins 0.000 claims 7
- 150000001413 amino acids Chemical class 0.000 claims 6
- 108060002716 Exonuclease Proteins 0.000 claims 4
- 102000013165 exonuclease Human genes 0.000 claims 4
- 108020004414 DNA Proteins 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 3
- 101100316841 Escherichia phage lambda bet gene Proteins 0.000 claims 3
- 101100226347 Escherichia phage lambda exo gene Proteins 0.000 claims 3
- 108010042407 Endonucleases Proteins 0.000 claims 2
- 102000004533 Endonucleases Human genes 0.000 claims 2
- 238000010459 TALEN Methods 0.000 claims 2
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 238000001727 in vivo Methods 0.000 claims 2
- 230000001404 mediated effect Effects 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 230000007018 DNA scission Effects 0.000 claims 1
- 101000889905 Enterobacteria phage RB3 Intron-associated endonuclease 3 Proteins 0.000 claims 1
- 101000889904 Enterobacteria phage T4 Defective intron-associated endonuclease 3 Proteins 0.000 claims 1
- 101000889900 Enterobacteria phage T4 Intron-associated endonuclease 1 Proteins 0.000 claims 1
- 101000889899 Enterobacteria phage T4 Intron-associated endonuclease 2 Proteins 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 102000001218 Rec A Recombinases Human genes 0.000 claims 1
- 108010055016 Rec A Recombinases Proteins 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 238000010367 cloning Methods 0.000 claims 1
- 238000004520 electroporation Methods 0.000 claims 1
- 108010050663 endodeoxyribonuclease CreI Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000013598 vector Substances 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1009732.7A GB201009732D0 (en) | 2010-06-10 | 2010-06-10 | Direct cloning |
| GB1009732.7 | 2010-06-10 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013513806A Division JP5934196B2 (ja) | 2010-06-10 | 2011-06-10 | 直接クローニング |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016135158A JP2016135158A (ja) | 2016-07-28 |
| JP2016135158A5 true JP2016135158A5 (https=) | 2016-09-08 |
Family
ID=42471456
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013513806A Active JP5934196B2 (ja) | 2010-06-10 | 2011-06-10 | 直接クローニング |
| JP2016093169A Pending JP2016135158A (ja) | 2010-06-10 | 2016-05-06 | 直接クローニング |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013513806A Active JP5934196B2 (ja) | 2010-06-10 | 2011-06-10 | 直接クローニング |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10443051B2 (https=) |
| EP (2) | EP3138921A1 (https=) |
| JP (2) | JP5934196B2 (https=) |
| CN (2) | CN109825486A (https=) |
| CA (1) | CA2802167C (https=) |
| GB (1) | GB201009732D0 (https=) |
| SG (1) | SG186219A1 (https=) |
| WO (1) | WO2011154927A2 (https=) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201009732D0 (en) * | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| EP2612918A1 (en) * | 2012-01-06 | 2013-07-10 | BASF Plant Science Company GmbH | In planta recombination |
| CN102558309B (zh) * | 2012-02-10 | 2014-09-17 | 浙江大学 | 一对转录激活子样效应因子核酸酶及其编码基因与应用 |
| CN102627691B (zh) * | 2012-04-05 | 2014-09-17 | 浙江大学 | 一对转录激活子样效应因子核酸酶及其编码基因与应用 |
| SG11201406547YA (en) * | 2012-04-25 | 2014-11-27 | Regeneron Pharma | Nuclease-mediated targeting with large targeting vectors |
| CN102702335B (zh) * | 2012-05-23 | 2014-07-09 | 上海斯丹赛生物技术有限公司 | 重组转录激活子样效应因子、转录激活子样效应因子核酸酶及其编码基因及应用 |
| GB2520903A (en) | 2012-10-16 | 2015-06-03 | Gene Bridges Gmbh | Electrocompetent cells and preparation thereof |
| DK3456831T3 (da) | 2013-04-16 | 2021-09-06 | Regeneron Pharma | Målrettet modifikation af rottegenom |
| KR102170502B1 (ko) | 2013-12-11 | 2020-10-28 | 리제너론 파마슈티칼스 인코포레이티드 | 게놈의 표적화된 변형을 위한 방법 및 조성물 |
| EP3083958B1 (en) * | 2013-12-19 | 2019-04-17 | Amyris, Inc. | Methods for genomic integration |
| HRP20200529T1 (hr) | 2014-06-06 | 2020-09-04 | Regeneron Pharmaceuticals, Inc. | Postupci i pripravci za modificiranje ciljnog lokusa |
| ES2781323T3 (es) | 2014-06-23 | 2020-09-01 | Regeneron Pharma | Montaje de ADN mediado por nucleasas |
| SI3161128T1 (sl) | 2014-06-26 | 2019-02-28 | Regeneron Pharmaceuticals, Inc. | Postopki in sestavki za ciljano genetsko spremembo in postopki uporabe |
| KR102531016B1 (ko) | 2014-11-21 | 2023-05-10 | 리제너론 파마슈티칼스 인코포레이티드 | 쌍 형성된 가이드 rna를 사용하는 표적화된 유전자 변형을 위한 방법 및 조성물 |
| CN105713885B (zh) * | 2014-12-04 | 2019-06-11 | 清华大学 | 特异识别并修复β地中海贫血症beta-globin基因的嵌合核酸酶 |
| ES2947714T3 (es) * | 2014-12-19 | 2023-08-17 | Regeneron Pharma | Métodos y composiciones para la modificación genética dirigida a través de múltiples direccionamientos en un solo paso |
| CN105734061B (zh) * | 2016-03-25 | 2019-07-23 | 中国农业科学院生物技术研究所 | 从毕赤酵母中分离的鼠李糖诱导型启动子及其应用 |
| EP3440213A1 (en) * | 2016-04-06 | 2019-02-13 | Gene Bridges GmbH | Adenoviral vectors |
| CN106995813B (zh) * | 2017-03-23 | 2020-06-16 | 山东大学 | 基因组大片段直接克隆和dna多分子组装新技术 |
| CN109385417A (zh) * | 2017-08-03 | 2019-02-26 | 华东理工大学 | 体内dna无缝组装方法 |
| KR102931179B1 (ko) * | 2019-04-05 | 2026-02-25 | 다니스코 유에스 인크. | 선형 재조합 dna 작제물 및 이의 조성물을 이용하여 공여 dna 서열을 바실러스 게놈 내에 통합시키기 위한 방법 |
| CN110184287B (zh) * | 2019-05-24 | 2024-01-30 | 华南农业大学 | 一种制备重组病毒的方法及其应用 |
| CN114630910A (zh) | 2019-06-25 | 2022-06-14 | 伊纳瑞农业技术有限公司 | 改善的同源依赖修复基因组编辑 |
| CN115717152A (zh) * | 2022-11-29 | 2023-02-28 | 江南大学 | 一种控制枯草芽孢杆菌基因组同源重组概率的方法 |
| EP4715055A1 (en) | 2024-09-19 | 2026-03-25 | European Molecular Biology Laboratory | Method for high efficiency bacterial in vivo or in vitro cloning |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| US5776744A (en) * | 1995-06-07 | 1998-07-07 | Yale University | Methods and compositions for effecting homologous recombination |
| US6509156B1 (en) | 1997-12-05 | 2003-01-21 | Europaisches Laboratorium Fur Molekularoiologie (Embl) | DNA cloning method relying on the E. coli recE/recT recombination system |
| AUPP684998A0 (en) * | 1998-10-30 | 1998-11-26 | Murdoch Institute for Research into Birth Defects Limited, The | A method of recombination and agents useful for same |
| US6355412B1 (en) * | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
| US6569681B1 (en) * | 2000-03-14 | 2003-05-27 | Transkaryotic Therapies, Inc. | Methods of improving homologous recombination |
| WO2002062988A2 (en) | 2001-02-09 | 2002-08-15 | Gene Bridges Gmbh | Recombination method |
| EP1409702A1 (en) * | 2001-07-20 | 2004-04-21 | Youming Zhang | Improved rect or recet cloning and subcloning method |
| EP3202899B1 (en) * | 2003-01-28 | 2020-10-21 | Cellectis | Custom-made meganuclease and use thereof |
| US20070155014A1 (en) | 2005-07-20 | 2007-07-05 | Invitrogen Corporation | Methods for increasing efficiency of homologous recombination |
| KR100681815B1 (ko) * | 2005-12-02 | 2007-02-12 | 한국생명공학연구원 | 리컴비네이즈 매개 선택표지 유전자 발현유도에 의한 목표유전자 간편 클로닝 및 목표 단백질 신속 발현 방법. |
| US10407672B2 (en) * | 2006-03-27 | 2019-09-10 | Seattle Children's Hospital | Compositions and methods comprising the use of cell surface displayed homing endonucleases |
| GB0803109D0 (en) * | 2008-02-20 | 2008-03-26 | Gene Bridges Gmbh | Method of nucleic acid recombination |
| JP2011217736A (ja) * | 2010-03-26 | 2011-11-04 | Aichi Medical Univ | 組換えクラミジア及びその製造方法 |
| GB201009732D0 (en) * | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
-
2010
- 2010-06-10 GB GBGB1009732.7A patent/GB201009732D0/en not_active Ceased
-
2011
- 2011-06-10 CN CN201810875866.5A patent/CN109825486A/zh active Pending
- 2011-06-10 WO PCT/IB2011/052549 patent/WO2011154927A2/en not_active Ceased
- 2011-06-10 CN CN201180038908.6A patent/CN103068995B/zh active Active
- 2011-06-10 EP EP16192084.8A patent/EP3138921A1/en not_active Withdrawn
- 2011-06-10 SG SG2012090056A patent/SG186219A1/en unknown
- 2011-06-10 EP EP11729474.4A patent/EP2580338B1/en active Active
- 2011-06-10 CA CA2802167A patent/CA2802167C/en active Active
- 2011-06-10 JP JP2013513806A patent/JP5934196B2/ja active Active
- 2011-06-10 US US13/806,086 patent/US10443051B2/en active Active
-
2016
- 2016-05-06 JP JP2016093169A patent/JP2016135158A/ja active Pending
-
2019
- 2019-09-06 US US16/563,274 patent/US12195732B2/en active Active
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