SG186219A1 - Direct cloning - Google Patents
Direct cloning Download PDFInfo
- Publication number
- SG186219A1 SG186219A1 SG2012090056A SG2012090056A SG186219A1 SG 186219 A1 SG186219 A1 SG 186219A1 SG 2012090056 A SG2012090056 A SG 2012090056A SG 2012090056 A SG2012090056 A SG 2012090056A SG 186219 A1 SG186219 A1 SG 186219A1
- Authority
- SG
- Singapore
- Prior art keywords
- nucleic acid
- acid molecule
- rece
- linear
- homologous recombination
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/16—Exonucleases active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters (3.16)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1009732.7A GB201009732D0 (en) | 2010-06-10 | 2010-06-10 | Direct cloning |
| PCT/IB2011/052549 WO2011154927A2 (en) | 2010-06-10 | 2011-06-10 | Direct cloning |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG186219A1 true SG186219A1 (en) | 2013-01-30 |
Family
ID=42471456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG2012090056A SG186219A1 (en) | 2010-06-10 | 2011-06-10 | Direct cloning |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10443051B2 (https=) |
| EP (2) | EP3138921A1 (https=) |
| JP (2) | JP5934196B2 (https=) |
| CN (2) | CN109825486A (https=) |
| CA (1) | CA2802167C (https=) |
| GB (1) | GB201009732D0 (https=) |
| SG (1) | SG186219A1 (https=) |
| WO (1) | WO2011154927A2 (https=) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201009732D0 (en) * | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| EP2612918A1 (en) * | 2012-01-06 | 2013-07-10 | BASF Plant Science Company GmbH | In planta recombination |
| CN102558309B (zh) * | 2012-02-10 | 2014-09-17 | 浙江大学 | 一对转录激活子样效应因子核酸酶及其编码基因与应用 |
| CN102627691B (zh) * | 2012-04-05 | 2014-09-17 | 浙江大学 | 一对转录激活子样效应因子核酸酶及其编码基因与应用 |
| SG11201406547YA (en) * | 2012-04-25 | 2014-11-27 | Regeneron Pharma | Nuclease-mediated targeting with large targeting vectors |
| CN102702335B (zh) * | 2012-05-23 | 2014-07-09 | 上海斯丹赛生物技术有限公司 | 重组转录激活子样效应因子、转录激活子样效应因子核酸酶及其编码基因及应用 |
| GB2520903A (en) | 2012-10-16 | 2015-06-03 | Gene Bridges Gmbh | Electrocompetent cells and preparation thereof |
| DK3456831T3 (da) | 2013-04-16 | 2021-09-06 | Regeneron Pharma | Målrettet modifikation af rottegenom |
| KR102170502B1 (ko) | 2013-12-11 | 2020-10-28 | 리제너론 파마슈티칼스 인코포레이티드 | 게놈의 표적화된 변형을 위한 방법 및 조성물 |
| EP3083958B1 (en) * | 2013-12-19 | 2019-04-17 | Amyris, Inc. | Methods for genomic integration |
| HRP20200529T1 (hr) | 2014-06-06 | 2020-09-04 | Regeneron Pharmaceuticals, Inc. | Postupci i pripravci za modificiranje ciljnog lokusa |
| ES2781323T3 (es) | 2014-06-23 | 2020-09-01 | Regeneron Pharma | Montaje de ADN mediado por nucleasas |
| SI3161128T1 (sl) | 2014-06-26 | 2019-02-28 | Regeneron Pharmaceuticals, Inc. | Postopki in sestavki za ciljano genetsko spremembo in postopki uporabe |
| KR102531016B1 (ko) | 2014-11-21 | 2023-05-10 | 리제너론 파마슈티칼스 인코포레이티드 | 쌍 형성된 가이드 rna를 사용하는 표적화된 유전자 변형을 위한 방법 및 조성물 |
| CN105713885B (zh) * | 2014-12-04 | 2019-06-11 | 清华大学 | 特异识别并修复β地中海贫血症beta-globin基因的嵌合核酸酶 |
| ES2947714T3 (es) * | 2014-12-19 | 2023-08-17 | Regeneron Pharma | Métodos y composiciones para la modificación genética dirigida a través de múltiples direccionamientos en un solo paso |
| CN105734061B (zh) * | 2016-03-25 | 2019-07-23 | 中国农业科学院生物技术研究所 | 从毕赤酵母中分离的鼠李糖诱导型启动子及其应用 |
| EP3440213A1 (en) * | 2016-04-06 | 2019-02-13 | Gene Bridges GmbH | Adenoviral vectors |
| CN106995813B (zh) * | 2017-03-23 | 2020-06-16 | 山东大学 | 基因组大片段直接克隆和dna多分子组装新技术 |
| CN109385417A (zh) * | 2017-08-03 | 2019-02-26 | 华东理工大学 | 体内dna无缝组装方法 |
| KR102931179B1 (ko) * | 2019-04-05 | 2026-02-25 | 다니스코 유에스 인크. | 선형 재조합 dna 작제물 및 이의 조성물을 이용하여 공여 dna 서열을 바실러스 게놈 내에 통합시키기 위한 방법 |
| CN110184287B (zh) * | 2019-05-24 | 2024-01-30 | 华南农业大学 | 一种制备重组病毒的方法及其应用 |
| CN114630910A (zh) | 2019-06-25 | 2022-06-14 | 伊纳瑞农业技术有限公司 | 改善的同源依赖修复基因组编辑 |
| CN115717152A (zh) * | 2022-11-29 | 2023-02-28 | 江南大学 | 一种控制枯草芽孢杆菌基因组同源重组概率的方法 |
| EP4715055A1 (en) | 2024-09-19 | 2026-03-25 | European Molecular Biology Laboratory | Method for high efficiency bacterial in vivo or in vitro cloning |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| US5776744A (en) * | 1995-06-07 | 1998-07-07 | Yale University | Methods and compositions for effecting homologous recombination |
| US6509156B1 (en) | 1997-12-05 | 2003-01-21 | Europaisches Laboratorium Fur Molekularoiologie (Embl) | DNA cloning method relying on the E. coli recE/recT recombination system |
| AUPP684998A0 (en) * | 1998-10-30 | 1998-11-26 | Murdoch Institute for Research into Birth Defects Limited, The | A method of recombination and agents useful for same |
| US6355412B1 (en) * | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
| US6569681B1 (en) * | 2000-03-14 | 2003-05-27 | Transkaryotic Therapies, Inc. | Methods of improving homologous recombination |
| WO2002062988A2 (en) | 2001-02-09 | 2002-08-15 | Gene Bridges Gmbh | Recombination method |
| EP1409702A1 (en) * | 2001-07-20 | 2004-04-21 | Youming Zhang | Improved rect or recet cloning and subcloning method |
| EP3202899B1 (en) * | 2003-01-28 | 2020-10-21 | Cellectis | Custom-made meganuclease and use thereof |
| US20070155014A1 (en) | 2005-07-20 | 2007-07-05 | Invitrogen Corporation | Methods for increasing efficiency of homologous recombination |
| KR100681815B1 (ko) * | 2005-12-02 | 2007-02-12 | 한국생명공학연구원 | 리컴비네이즈 매개 선택표지 유전자 발현유도에 의한 목표유전자 간편 클로닝 및 목표 단백질 신속 발현 방법. |
| US10407672B2 (en) * | 2006-03-27 | 2019-09-10 | Seattle Children's Hospital | Compositions and methods comprising the use of cell surface displayed homing endonucleases |
| GB0803109D0 (en) * | 2008-02-20 | 2008-03-26 | Gene Bridges Gmbh | Method of nucleic acid recombination |
| JP2011217736A (ja) * | 2010-03-26 | 2011-11-04 | Aichi Medical Univ | 組換えクラミジア及びその製造方法 |
| GB201009732D0 (en) * | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
-
2010
- 2010-06-10 GB GBGB1009732.7A patent/GB201009732D0/en not_active Ceased
-
2011
- 2011-06-10 CN CN201810875866.5A patent/CN109825486A/zh active Pending
- 2011-06-10 WO PCT/IB2011/052549 patent/WO2011154927A2/en not_active Ceased
- 2011-06-10 CN CN201180038908.6A patent/CN103068995B/zh active Active
- 2011-06-10 EP EP16192084.8A patent/EP3138921A1/en not_active Withdrawn
- 2011-06-10 SG SG2012090056A patent/SG186219A1/en unknown
- 2011-06-10 EP EP11729474.4A patent/EP2580338B1/en active Active
- 2011-06-10 CA CA2802167A patent/CA2802167C/en active Active
- 2011-06-10 JP JP2013513806A patent/JP5934196B2/ja active Active
- 2011-06-10 US US13/806,086 patent/US10443051B2/en active Active
-
2016
- 2016-05-06 JP JP2016093169A patent/JP2016135158A/ja active Pending
-
2019
- 2019-09-06 US US16/563,274 patent/US12195732B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN109825486A (zh) | 2019-05-31 |
| EP3138921A1 (en) | 2017-03-08 |
| US12195732B2 (en) | 2025-01-14 |
| US20130210681A1 (en) | 2013-08-15 |
| CA2802167A1 (en) | 2011-12-15 |
| WO2011154927A9 (en) | 2013-01-24 |
| WO2011154927A2 (en) | 2011-12-15 |
| CN103068995B (zh) | 2020-10-16 |
| JP2016135158A (ja) | 2016-07-28 |
| JP2013530689A (ja) | 2013-08-01 |
| CA2802167C (en) | 2023-03-07 |
| US20200283759A1 (en) | 2020-09-10 |
| US10443051B2 (en) | 2019-10-15 |
| WO2011154927A3 (en) | 2012-02-16 |
| GB201009732D0 (en) | 2010-07-21 |
| EP2580338A2 (en) | 2013-04-17 |
| JP5934196B2 (ja) | 2016-06-15 |
| EP2580338B1 (en) | 2016-10-05 |
| CN103068995A (zh) | 2013-04-24 |
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