JP2016067292A - 細胞培養観察装置および方法 - Google Patents
細胞培養観察装置および方法 Download PDFInfo
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- 238000004114 suspension culture Methods 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 description 113
- 239000002609 medium Substances 0.000 description 23
- 230000035945 sensitivity Effects 0.000 description 14
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- 239000001963 growth medium Substances 0.000 description 9
- 230000009087 cell motility Effects 0.000 description 5
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
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- 239000001569 carbon dioxide Substances 0.000 description 1
- 210000004413 cardiac myocyte Anatomy 0.000 description 1
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- 239000004065 semiconductor Substances 0.000 description 1
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Abstract
Description
2 入力装置
3 表示装置
10 培養部
11 ステージ
12 搬送部
13 培養制御部
20 操作部
21 回転テーブル
22 培地交換部
23 薬剤添加部
24 細胞移動部
25 回転テーブル駆動部
26 容器搬送制御部
30 細胞観察部
31 撮像部
32 光学系機構部
33 撮像制御部
34 細胞観察用ステージ
40 全体制御部
41 操作内容判定部
42 表示制御部
Claims (13)
- 細胞を培養する複数の培養容器内の前記細胞を培養容器毎に観察する細胞観察部と、
前記各培養容器に対して複数の操作を行う操作部と、
前記複数の操作のうち、観察対象とする前記培養容器の観察中に該観察対象とする培養容器以外の培養容器に対して実施可能な操作の内容を判定する操作内容判定部とを備えたことを特徴とする細胞培養観察装置。 - 前記操作内容判定部が、前記観察対象とする培養容器の観察条件に基づいて、前記実施可能な操作の内容を判定する請求項1記載の細胞培養観察装置。
- 前記観察条件に、前記細胞を撮像する撮像素子の露光時間、前記細胞を観察する光学系の倍率、前記細胞の撮像間隔、前記細胞の撮像回数および前記細胞の観察手法の少なくともうち1つが含まれる請求項2記載の細胞培養観察装置。
- 前記観察手法に、明視野観察、位相差観察、微分干渉観察および蛍光観察のうち少なくとも1つが含まれる請求項3記載の細胞培養観察装置。
- 前記操作内容判定部が、さらに前記細胞の種類に基づいて、前記実施可能な操作の内容を判定する請求項2から4いずれか1項記載の細胞培養観察装置。
- 前記操作内容判定部が、さらに前記細胞の培養条件に基づいて、前記実施可能な操作の内容を判定する請求項2から5いずれか1項記載の細胞培養観察装置。
- 前記培養条件に、平面培養、浮遊培養、立体培養、足場の種類、培地の種類、培地に添加する薬剤の種類の少なくとも1つが含まれる請求項6記載の細胞培養観察装置。
- 前記操作内容判定部が、前記観察条件に基づいて判定値を算出し、該判定値に基づいて前記実施可能な操作の内容を判定する請求項2から7いずれか1項記載の細胞培養観察装置。
- 前記操作の内容に、培地交換、培地への薬剤添加、培養容器の搬送、細胞の継代操作および細胞の剥離操作の少なくとも1つが含まれている請求項1から8いずれか1項記載の細胞培養観察装置。
- 前記細胞を培養する培養部と前記観察部との間において前記培養容器を搬送する搬送部を備えた請求項1から9いずれか1項記載の細胞培養観察装置。
- 前記実施可能な操作の内容を表示させる表示制御部を備えた請求項1から10いずれか1項記載の細胞培養観察装置。
- 前記操作内容判定部において判定された操作の内容に基づいて、前記観察対象の培養容器の観察タイミングと前記観察対象の培養容器以外の培養容器に対する操作タイミングとを決定する制御部を備えた請求項1から11いずれか1項記載の細胞培養観察装置。
- 細胞を培養する複数の培養容器内の前記細胞を培養容器毎に観察し、かつ前記各培養容器に対して複数の操作を行う細胞培養観察方法において、
前記複数の操作のうち、観察対象とする前記培養容器の観察中に該観察対象とする培養容器以外の培養容器に対して実施可能な操作の内容を判定することを特徴とする細胞培養観察方法。
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JP2014200942A JP6277103B2 (ja) | 2014-09-30 | 2014-09-30 | 細胞培養観察装置および方法 |
EP15847723.2A EP3202888B1 (en) | 2014-09-30 | 2015-09-17 | Cell culture observation device and method |
PCT/JP2015/076554 WO2016052238A1 (ja) | 2014-09-30 | 2015-09-17 | 細胞培養観察装置および方法 |
US15/439,318 US10344255B2 (en) | 2014-09-30 | 2017-02-22 | Cell culture observation device and method to determine a content operation |
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Citations (6)
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JP2006011415A (ja) * | 2004-05-26 | 2006-01-12 | Olympus Corp | 培養顕微鏡、及び、培養顕微鏡を制御するコンピュータプログラム |
JP2006171227A (ja) * | 2004-12-14 | 2006-06-29 | Olympus Corp | 観察装置 |
JP2006314214A (ja) * | 2005-05-10 | 2006-11-24 | Olympus Corp | 細胞観察装置、細胞観察方法、及び細胞観察プログラム |
JP2009291103A (ja) * | 2008-06-04 | 2009-12-17 | Kawasaki Heavy Ind Ltd | 自動細胞培養装置 |
JP2010220511A (ja) * | 2009-03-23 | 2010-10-07 | Nikon Corp | 培養観察装置及び情報管理方法 |
JP2012200181A (ja) * | 2011-03-24 | 2012-10-22 | Nikon Corp | 培養装置、培養管理方法およびプログラム |
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WO2009031283A1 (ja) * | 2007-09-03 | 2009-03-12 | Nikon Corporation | 培養装置、培養情報管理方法およびプログラム |
EP2213722B1 (en) * | 2007-10-19 | 2018-01-10 | Nikon Corporation | Program, computer, and culture state analyzing method |
WO2012050645A2 (en) * | 2010-06-25 | 2012-04-19 | Purdue Research Foundation | Pathogen detection |
WO2012127879A1 (ja) * | 2011-03-24 | 2012-09-27 | 株式会社ニコン | 培養装置、培養装置システム、培養操作管理方法およびプログラム |
US20140363838A1 (en) * | 2013-06-11 | 2014-12-11 | William Marsh Rice University | Microperfusion imaging platform |
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JP2006011415A (ja) * | 2004-05-26 | 2006-01-12 | Olympus Corp | 培養顕微鏡、及び、培養顕微鏡を制御するコンピュータプログラム |
JP2006171227A (ja) * | 2004-12-14 | 2006-06-29 | Olympus Corp | 観察装置 |
JP2006314214A (ja) * | 2005-05-10 | 2006-11-24 | Olympus Corp | 細胞観察装置、細胞観察方法、及び細胞観察プログラム |
JP2009291103A (ja) * | 2008-06-04 | 2009-12-17 | Kawasaki Heavy Ind Ltd | 自動細胞培養装置 |
JP2010220511A (ja) * | 2009-03-23 | 2010-10-07 | Nikon Corp | 培養観察装置及び情報管理方法 |
JP2012200181A (ja) * | 2011-03-24 | 2012-10-22 | Nikon Corp | 培養装置、培養管理方法およびプログラム |
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EP3202888B1 (en) | 2018-12-12 |
US10344255B2 (en) | 2019-07-09 |
WO2016052238A1 (ja) | 2016-04-07 |
EP3202888A4 (en) | 2017-10-18 |
EP3202888A1 (en) | 2017-08-09 |
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