JP2015526069A5 - - Google Patents

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Publication number
JP2015526069A5
JP2015526069A5 JP2015520511A JP2015520511A JP2015526069A5 JP 2015526069 A5 JP2015526069 A5 JP 2015526069A5 JP 2015520511 A JP2015520511 A JP 2015520511A JP 2015520511 A JP2015520511 A JP 2015520511A JP 2015526069 A5 JP2015526069 A5 JP 2015526069A5
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JP
Japan
Prior art keywords
inosine
reverse transcriptase
dna
primers
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
JP2015520511A
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English (en)
Japanese (ja)
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JP2015526069A (ja
JP6382189B2 (ja
Filing date
Publication date
Priority claimed from US13/538,955 external-priority patent/US9777319B2/en
Priority claimed from US13/538,962 external-priority patent/US20140004509A1/en
Application filed filed Critical
Priority claimed from PCT/US2013/048229 external-priority patent/WO2014004852A2/en
Publication of JP2015526069A publication Critical patent/JP2015526069A/ja
Publication of JP2015526069A5 publication Critical patent/JP2015526069A5/ja
Application granted granted Critical
Publication of JP6382189B2 publication Critical patent/JP6382189B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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JP2015520511A 2012-06-29 2013-06-27 Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット Active JP6382189B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US13/538,955 2012-06-29
US13/538,955 US9777319B2 (en) 2012-06-29 2012-06-29 Method for isothermal DNA amplification starting from an RNA template
US13/538,962 US20140004509A1 (en) 2012-06-29 2012-06-29 Kit for isothermal dna amplification starting from an rna template
US13/538,962 2012-06-29
PCT/US2013/048229 WO2014004852A2 (en) 2012-06-29 2013-06-27 Kit for isothermal dna amplification starting from an rna template

Publications (3)

Publication Number Publication Date
JP2015526069A JP2015526069A (ja) 2015-09-10
JP2015526069A5 true JP2015526069A5 (https=) 2016-08-12
JP6382189B2 JP6382189B2 (ja) 2018-08-29

Family

ID=49784019

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015520511A Active JP6382189B2 (ja) 2012-06-29 2013-06-27 Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット

Country Status (5)

Country Link
EP (1) EP2867366B1 (https=)
JP (1) JP6382189B2 (https=)
CN (1) CN104583413B (https=)
CA (1) CA2877368C (https=)
WO (1) WO2014004852A2 (https=)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130323795A1 (en) 2007-01-10 2013-12-05 General Electric Company Endonuclase-assisted isothermal amplification using contamination-free reagents
WO2012021493A2 (en) 2010-08-13 2012-02-16 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
RU2601129C2 (ru) 2012-04-09 2016-10-27 Инвайролоджикс Инк. Композиции и способы для количественного определения последовательности нуклеиновой кислоты в образце
EP3033431B1 (en) * 2013-08-13 2019-10-02 General Electric Company Endonuclease-assisted isothermal amplification using contamination-free reagents
HRP20210953T1 (hr) * 2014-02-18 2021-09-03 Illumina, Inc. Postupci i pripravci za određivanje dnk profila
EP3748011B1 (en) 2014-04-22 2024-11-13 Envirologix Inc. Compositions and methods for enhancing and/or predicting dna amplification
CN107208137A (zh) * 2014-10-20 2017-09-26 龙公司 检测rna病毒的组合物和方法
ES2898103T3 (es) 2014-12-31 2022-03-03 Visby Medical Inc Dispositivos para pruebas de diagnóstico molecular
CA3024546A1 (en) * 2016-05-17 2017-11-23 Masayuki SU'ETSUGU Method of amplifying circular dna
CN107400701A (zh) * 2016-05-18 2017-11-28 上海德沛生物科技有限公司 环状rna分子的扩增和测序方法
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
CA3029682A1 (en) * 2016-06-30 2018-01-04 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
KR20200079264A (ko) 2017-11-09 2020-07-02 비스비 메디컬, 인코포레이티드 표적 바이러스 검출을 위한 휴대용 분자 진단 디바이스 및 방법
JP7029138B2 (ja) * 2018-03-05 2022-03-03 国立大学法人埼玉大学 連結核酸断片の製造方法、連結核酸断片、及び連結核酸断片から構成されるライブラリ
CN110819673B (zh) * 2018-08-07 2021-07-13 华中农业大学 一种原核生物cDNA5′末端快速扩增方法
WO2020132714A1 (en) * 2018-12-24 2020-07-02 Deakin University Method for amplification of nucleic acids
MX2023010495A (es) * 2021-03-30 2023-09-18 Illumina Inc Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos.
CN114891860A (zh) * 2022-03-31 2022-08-12 河南省人民医院 一种基于crispr和hrca技术的可视化靶dna检测方法
CN114921528A (zh) * 2022-04-22 2022-08-19 山东见微生物科技有限公司 一种核酸内切酶4介导的rna特异扩增方法
CN116004681B (zh) * 2022-08-15 2023-08-29 南京诺唯赞生物科技股份有限公司 一种提高topo克隆中载体连接效率的方法及试剂盒

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
CA2598508A1 (fr) * 2005-02-25 2006-08-31 Commissariat A L'energie Atomique Procede et dispositif pour separer des cibles moleculaires dans un melange complexe
CA2609218C (en) * 2005-05-26 2016-10-11 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
JP4918409B2 (ja) * 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US8202972B2 (en) * 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
CA2749939A1 (en) * 2009-01-21 2010-07-29 Human Genetic Signatures Pty Ltd Improved isothermal strand displacement amplification
JP2012080871A (ja) * 2009-12-14 2012-04-26 National Agriculture & Food Research Organization Ｒｎａの直接検出法
WO2014164479A1 (en) * 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification

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