JP2015521469A5 - - Google Patents
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- JP2015521469A5 JP2015521469A5 JP2015517358A JP2015517358A JP2015521469A5 JP 2015521469 A5 JP2015521469 A5 JP 2015521469A5 JP 2015517358 A JP2015517358 A JP 2015517358A JP 2015517358 A JP2015517358 A JP 2015517358A JP 2015521469 A5 JP2015521469 A5 JP 2015521469A5
- Authority
- JP
- Japan
- Prior art keywords
- mnp
- hesc
- culture
- ipsc
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 claims description 55
- 239000002609 medium Substances 0.000 claims description 38
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 28
- 230000006698 induction Effects 0.000 claims description 26
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 17
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 claims description 16
- 230000001464 adherent effect Effects 0.000 claims description 16
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 14
- 108010010803 Gelatin Proteins 0.000 claims description 12
- 229920000159 gelatin Polymers 0.000 claims description 12
- 239000008273 gelatin Substances 0.000 claims description 12
- 235000019322 gelatine Nutrition 0.000 claims description 12
- 235000011852 gelatine desserts Nutrition 0.000 claims description 12
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 8
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims description 8
- 239000006285 cell suspension Substances 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 8
- 239000012595 freezing medium Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- 210000005036 nerve Anatomy 0.000 claims description 8
- 229930002330 retinoic acid Natural products 0.000 claims description 8
- 238000001179 sorption measurement Methods 0.000 claims description 8
- 229960001727 tretinoin Drugs 0.000 claims description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 6
- 102000004877 Insulin Human genes 0.000 claims description 6
- 108090001061 Insulin Proteins 0.000 claims description 6
- 102000004338 Transferrin Human genes 0.000 claims description 6
- 108090000901 Transferrin Proteins 0.000 claims description 6
- 102000004142 Trypsin Human genes 0.000 claims description 6
- 108090000631 Trypsin Proteins 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 210000002950 fibroblast Anatomy 0.000 claims description 6
- 229940125396 insulin Drugs 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 6
- 239000012581 transferrin Substances 0.000 claims description 6
- 239000012588 trypsin Substances 0.000 claims description 6
- ZOOGRGPOEVQQDX-UUOKFMHZSA-N 3',5'-cyclic GMP Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-UUOKFMHZSA-N 0.000 claims description 5
- 238000003306 harvesting Methods 0.000 claims description 5
- 238000010899 nucleation Methods 0.000 claims description 5
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 4
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 claims description 4
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 4
- 230000002255 enzymatic effect Effects 0.000 claims description 4
- 238000007710 freezing Methods 0.000 claims description 4
- 230000008014 freezing Effects 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 4
- 239000003102 growth factor Substances 0.000 claims description 4
- 238000000338 in vitro Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 210000002161 motor neuron Anatomy 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 229910052711 selenium Inorganic materials 0.000 claims description 4
- 239000011669 selenium Substances 0.000 claims description 4
- 210000000130 stem cell Anatomy 0.000 claims description 4
- 102000009027 Albumins Human genes 0.000 claims description 2
- 108010088751 Albumins Proteins 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 claims description 2
- 238000005138 cryopreservation Methods 0.000 claims description 2
- 230000002500 effect on skin Effects 0.000 claims description 2
- 239000003797 essential amino acid Substances 0.000 claims description 2
- 235000020776 essential amino acid Nutrition 0.000 claims description 2
- 210000003953 foreskin Anatomy 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 230000007774 longterm Effects 0.000 claims description 2
- 108010082117 matrigel Proteins 0.000 claims description 2
- 239000013049 sediment Substances 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- 238000010257 thawing Methods 0.000 claims description 2
- 230000035899 viability Effects 0.000 claims description 2
- 229930003231 vitamin Natural products 0.000 claims description 2
- 239000011782 vitamin Substances 0.000 claims description 2
- 229940088594 vitamin Drugs 0.000 claims description 2
- 235000013343 vitamin Nutrition 0.000 claims description 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261658061P | 2012-06-11 | 2012-06-11 | |
| US61/658,061 | 2012-06-11 | ||
| PCT/US2013/045184 WO2013188407A2 (en) | 2012-06-11 | 2013-06-11 | Method of in vitro differentiation of motor neuron progenitors (mnps) from human induced pluripotent stem cells and cryopreservation of mnps |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015521469A JP2015521469A (ja) | 2015-07-30 |
| JP2015521469A5 true JP2015521469A5 (https=) | 2016-07-14 |
Family
ID=49758856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015517358A Ceased JP2015521469A (ja) | 2012-06-11 | 2013-06-11 | ヒト人工多能性幹細胞からの運動ニューロン前駆細胞（ｍｎｐ）のインビトロ分化法およびｍｎｐの凍結保存法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20150159133A1 (https=) |
| EP (1) | EP2858581A4 (https=) |
| JP (1) | JP2015521469A (https=) |
| WO (1) | WO2013188407A2 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160130561A1 (en) * | 2013-06-13 | 2016-05-12 | The New York Stem Cell Foundation Inc. | Pluripotent stem cells derived from non-cryoprotected frozen tissue and methods for making and using the same |
| EP3491134B1 (en) | 2016-08-01 | 2023-10-11 | University of Pittsburgh - of The Commonwealth System of Higher Education | Human induced pluripotent stem cells for high efficiency genetic engineering |
| WO2020154631A1 (en) * | 2019-01-24 | 2020-07-30 | Northwestern University | Dynamics within supramolecular 1kvav matrices enhance functional maturation of human ipscs-der1ved neurons and regeneration |
| AU2020387259A1 (en) | 2019-11-20 | 2022-06-09 | Kyoto University | Method for freezing neural cells |
| CN119432743A (zh) * | 2023-07-28 | 2025-02-14 | 士泽生物医药（苏州）有限公司 | 一种诱导干细胞分化为运动神经前体细胞和/或运动神经元的试剂盒及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3660601B2 (ja) * | 2001-03-30 | 2005-06-15 | 独立行政法人科学技術振興機構 | 胚性幹細胞からの神経幹細胞、運動ニューロン及びｇａｂａ作動性ニューロンの製造法 |
| US8153424B2 (en) * | 2001-10-03 | 2012-04-10 | Wisconsin Alumni Research Foundation | Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells |
| US7285415B2 (en) * | 2002-07-11 | 2007-10-23 | The Regents Of The University Of California | Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury |
| US8263406B2 (en) * | 2003-06-11 | 2012-09-11 | Cornell Research Foundation, Inc. | Enriched or purified population of motor neurons and its preparation from a population of embryonic stem cells |
| WO2006044204A2 (en) * | 2004-10-05 | 2006-04-27 | University Of Georgia Research Foundation, Inc. | Neuronal progenitors from feeder-free human embryonic stem cell culture |
| US8137971B2 (en) * | 2007-03-23 | 2012-03-20 | California Stem Cell, Inc. | Preparing a population of human late stage motor neuron progenitor cells |
| EP2028268A1 (en) * | 2007-08-20 | 2009-02-25 | Université Libre De Bruxelles | Generation of neuronal cells from pluripotent stem cells |
| WO2009122413A1 (en) * | 2008-03-31 | 2009-10-08 | Hadasit Medical Research Services & Development Limited | Motor neurons developed from stem cells |
| JP2013507931A (ja) * | 2009-10-16 | 2013-03-07 | ユニバーシティ オブ メディスン アンド デンティストリー オブ ニュー ジャージー | 再生医療用途のための接着性骨髄幹細胞の閉鎖系統分離システム |
-
2013
- 2013-06-11 US US14/406,665 patent/US20150159133A1/en not_active Abandoned
- 2013-06-11 EP EP13803543.1A patent/EP2858581A4/en not_active Withdrawn
- 2013-06-11 WO PCT/US2013/045184 patent/WO2013188407A2/en not_active Ceased
- 2013-06-11 JP JP2015517358A patent/JP2015521469A/ja not_active Ceased
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