JP2015521469A - ヒト人工多能性幹細胞からの運動ニューロン前駆細胞（ｍｎｐ）のインビトロ分化法およびｍｎｐの凍結保存法 - Google Patents

ヒト人工多能性幹細胞からの運動ニューロン前駆細胞（ｍｎｐ）のインビトロ分化法およびｍｎｐの凍結保存法 Download PDF

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JP2015521469A
JP2015521469A JP2015517358A JP2015517358A JP2015521469A JP 2015521469 A JP2015521469 A JP 2015521469A JP 2015517358 A JP2015517358 A JP 2015517358A JP 2015517358 A JP2015517358 A JP 2015517358A JP 2015521469 A JP2015521469 A JP 2015521469A
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ヤン　ファン
ファン ヤン
ヒュアン トラン
ヒュアン トラン
トーマス フェルナー
トーマス フェルナー
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ロンザ ウォカーズビル インコーポレーティッド
ロンザ ウォカーズビル インコーポレーティッド
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Animal Behavior & Ethology (AREA)
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JP2015517358A 2012-06-11 2013-06-11 ヒト人工多能性幹細胞からの運動ニューロン前駆細胞（ｍｎｐ）のインビトロ分化法およびｍｎｐの凍結保存法 Ceased JP2015521469A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261658061P 2012-06-11 2012-06-11
US61/658,061 2012-06-11
PCT/US2013/045184 WO2013188407A2 (en) 2012-06-11 2013-06-11 Method of in vitro differentiation of motor neuron progenitors (mnps) from human induced pluripotent stem cells and cryopreservation of mnps

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JP2015521469A true JP2015521469A (ja) 2015-07-30
JP2015521469A5 JP2015521469A5 (https=) 2016-07-14

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JP2015517358A Ceased JP2015521469A (ja) 2012-06-11 2013-06-11 ヒト人工多能性幹細胞からの運動ニューロン前駆細胞（ｍｎｐ）のインビトロ分化法およびｍｎｐの凍結保存法

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US (1) US20150159133A1 (https=)
EP (1) EP2858581A4 (https=)
JP (1) JP2015521469A (https=)
WO (1) WO2013188407A2 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220104764A (ko) 2019-11-20 2022-07-26 스미토모 파마 가부시키가이샤 신경계 세포의 동결 방법
JP2024138368A (ja) * 2019-01-24 2024-10-08 ノースウエスタン ユニバーシティ 超分子ｉｋｖａｖマトリックス内の動力学は、ヒトｉｐｓｃ由来のニューロンの機能的成熟と再生を増強する

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160130561A1 (en) * 2013-06-13 2016-05-12 The New York Stem Cell Foundation Inc. Pluripotent stem cells derived from non-cryoprotected frozen tissue and methods for making and using the same
EP3491134B1 (en) 2016-08-01 2023-10-11 University of Pittsburgh - of The Commonwealth System of Higher Education Human induced pluripotent stem cells for high efficiency genetic engineering
CN119432743A (zh) * 2023-07-28 2025-02-14 士泽生物医药（苏州）有限公司 一种诱导干细胞分化为运动神经前体细胞和/或运动神经元的试剂盒及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008118421A1 (en) * 2007-03-23 2008-10-02 California Stem Cell, Inc. Human late stage motor neuron progenitor cells and methods of making and using same
JP2010536357A (ja) * 2007-08-20 2010-12-02 ユニヴァルシテ リブレ デ ブリュッセル 多能性幹細胞からのニューロン細胞の生成
WO2011047289A1 (en) * 2009-10-16 2011-04-21 University Of Medicine And Dentistry Of New Jersey Closed system separation of adherent bone marrow stem cells for regenerative medicine applications

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3660601B2 (ja) * 2001-03-30 2005-06-15 独立行政法人科学技術振興機構 胚性幹細胞からの神経幹細胞、運動ニューロン及びｇａｂａ作動性ニューロンの製造法
US8153424B2 (en) * 2001-10-03 2012-04-10 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
US7285415B2 (en) * 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US8263406B2 (en) * 2003-06-11 2012-09-11 Cornell Research Foundation, Inc. Enriched or purified population of motor neurons and its preparation from a population of embryonic stem cells
WO2006044204A2 (en) * 2004-10-05 2006-04-27 University Of Georgia Research Foundation, Inc. Neuronal progenitors from feeder-free human embryonic stem cell culture
WO2009122413A1 (en) * 2008-03-31 2009-10-08 Hadasit Medical Research Services & Development Limited Motor neurons developed from stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008118421A1 (en) * 2007-03-23 2008-10-02 California Stem Cell, Inc. Human late stage motor neuron progenitor cells and methods of making and using same
JP2010536357A (ja) * 2007-08-20 2010-12-02 ユニヴァルシテ リブレ デ ブリュッセル 多能性幹細胞からのニューロン細胞の生成
WO2011047289A1 (en) * 2009-10-16 2011-04-21 University Of Medicine And Dentistry Of New Jersey Closed system separation of adherent bone marrow stem cells for regenerative medicine applications

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024138368A (ja) * 2019-01-24 2024-10-08 ノースウエスタン ユニバーシティ 超分子ｉｋｖａｖマトリックス内の動力学は、ヒトｉｐｓｃ由来のニューロンの機能的成熟と再生を増強する
KR20220104764A (ko) 2019-11-20 2022-07-26 스미토모 파마 가부시키가이샤 신경계 세포의 동결 방법

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Publication number Publication date
WO2013188407A2 (en) 2013-12-19
WO2013188407A3 (en) 2015-05-07
EP2858581A4 (en) 2016-07-27
US20150159133A1 (en) 2015-06-11
EP2858581A2 (en) 2015-04-15

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