JP2015508705A5 - - Google Patents

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JP2015508705A5
JP2015508705A5 JP2014556753A JP2014556753A JP2015508705A5 JP 2015508705 A5 JP2015508705 A5 JP 2015508705A5 JP 2014556753 A JP2014556753 A JP 2014556753A JP 2014556753 A JP2014556753 A JP 2014556753A JP 2015508705 A5 JP2015508705 A5 JP 2015508705A5
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fluid
fluid line
pulses emitted
pulsed laser
opening
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JP6367717B2 (ja
JP2015508705A (ja
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JP2014556753A 2012-02-09 2013-02-08 誘発されたキャビテーションによって駆動される高速オンデマンド型液滴生成および単一細胞封入 Expired - Fee Related JP6367717B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/370,196 US9176504B2 (en) 2011-02-11 2012-02-09 High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation
US13/370,196 2012-02-09
PCT/US2013/025434 WO2013120016A2 (en) 2012-02-09 2013-02-08 High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation

Related Child Applications (1)

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JP2017172951A Division JP2018034154A (ja) 2012-02-09 2017-09-08 誘発されたキャビテーションによって駆動される高速オンデマンド型液滴生成および単一細胞封入

Publications (3)

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JP2015508705A JP2015508705A (ja) 2015-03-23
JP2015508705A5 true JP2015508705A5 (https=) 2016-03-31
JP6367717B2 JP6367717B2 (ja) 2018-08-01

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JP2014556753A Expired - Fee Related JP6367717B2 (ja) 2012-02-09 2013-02-08 誘発されたキャビテーションによって駆動される高速オンデマンド型液滴生成および単一細胞封入
JP2017172951A Pending JP2018034154A (ja) 2012-02-09 2017-09-08 誘発されたキャビテーションによって駆動される高速オンデマンド型液滴生成および単一細胞封入

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JP2017172951A Pending JP2018034154A (ja) 2012-02-09 2017-09-08 誘発されたキャビテーションによって駆動される高速オンデマンド型液滴生成および単一細胞封入

Country Status (9)

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US (2) US9176504B2 (https=)
EP (2) EP2812709B1 (https=)
JP (2) JP6367717B2 (https=)
KR (1) KR20150016929A (https=)
CN (2) CN104321652B (https=)
AU (2) AU2013216804B2 (https=)
CA (1) CA2864138C (https=)
ES (2) ES2832486T3 (https=)
WO (1) WO2013120016A2 (https=)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9364831B2 (en) 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
US9176504B2 (en) 2011-02-11 2015-11-03 The Regents Of The University Of California High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation
US10564147B2 (en) 2012-05-25 2020-02-18 The Regents Of The University Of California Microfluidic systems for particle trapping and separation using cavity acoustic transducers
US9103468B2 (en) * 2012-07-30 2015-08-11 University Of South Carolina Enhanced flow boiling in microchannels by high frequency microbubble-excited and -modulated oscillations
US9731293B2 (en) * 2012-10-03 2017-08-15 The United States Of America, As Represented By The Secretary Of The Navy Paired laser and electrokinetic separation, manipulation, and analysis device
US20140151229A1 (en) 2012-12-05 2014-06-05 Caliper Life Sciences, Inc. Manipulation of objects in microfluidic devices using external electrodes
US9297784B2 (en) * 2012-12-05 2016-03-29 Caliper Life Sciences, Inc. Device and method for extracting target objects from a sample
US9995412B2 (en) * 2013-03-01 2018-06-12 Wave 80 Biosciences, Inc. Long-throw microfluidic actuator
EP2972405B1 (en) * 2013-03-15 2020-12-23 The Regents of The University of California High-speed on demand microfluidic droplet generation and manipulation
EP2848698A1 (en) * 2013-08-26 2015-03-18 F. Hoffmann-La Roche AG System and method for automated nucleic acid amplification
CN103614290B (zh) * 2013-11-11 2015-03-04 北京工业大学 面向荧光pcr微系统的往复式循环单微通道装置
US10960396B2 (en) 2014-05-16 2021-03-30 Cytonome/St, Llc Thermal activated microfluidic switching
CA2890401C (en) 2015-01-21 2015-11-03 Vln Advanced Technologies Inc. Electrodischarge apparatus for generating low-frequency powerful pulsed and cavitating waterjets
WO2016176663A1 (en) 2015-04-29 2016-11-03 Flodesign Sonics, Inc. Acoustophoretic device for angled wave particle deflection
CN104941702B (zh) * 2015-05-25 2017-01-04 重庆大学 一种微通道内产生超小液滴的方法
WO2017004676A1 (en) * 2015-07-09 2017-01-12 Monash University On-demand single cell encapsulation using a localised acoustic field
US9862941B2 (en) 2015-10-14 2018-01-09 Pioneer Hi-Bred International, Inc. Single cell microfluidic device
CA2921675C (en) 2016-02-24 2017-12-05 Vln Advanced Technologies Inc. Electro-discharge system for neutralizing landmines
FR3049348B1 (fr) * 2016-03-23 2023-08-11 Commissariat Energie Atomique Procede de caracterisation d’une particule dans un echantillon
US12345708B2 (en) * 2016-03-25 2025-07-01 President And Fellows Of Harvard College Microfluidic determination of immune and other cells
US10228317B1 (en) * 2016-08-25 2019-03-12 Verily Life Sciences Llc Multiplexed microfluidic cell sorting using laser induced cavitation bubbles
CN106190779B (zh) * 2016-08-29 2018-08-24 苏州汶颢芯片科技有限公司 基于微流控芯片的单细胞分离和包封装置和方法
WO2018071448A1 (en) 2016-10-11 2018-04-19 The Regents Of The University Of California Systems and methods to encapsulate and preserve organic matter for analysis
EP3471865A4 (en) * 2016-10-21 2019-08-14 Hewlett-Packard Development Company, L.P. DROPLET GENERATOR
US10718004B2 (en) 2016-12-20 2020-07-21 Lawrence Livermore National Security, Llc Droplet array for single-cell analysis
EP3558524A1 (en) * 2016-12-22 2019-10-30 IMEC vzw A microfluidic device for sorting out droplets
CN106890683B (zh) * 2017-03-14 2019-03-08 广东工业大学 一种多层液滴包裹的形成方法
CN110945139B (zh) 2017-05-18 2023-09-05 10X基因组学有限公司 用于分选液滴和珠的方法和系统
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
WO2018227204A1 (en) * 2017-06-09 2018-12-13 The Regents Of The University Of California Controlled encapsulation in droplets by liquid-liquid interfacial shearing
US11517901B2 (en) 2017-06-09 2022-12-06 The Regents Of The University Of California High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting
US20190064173A1 (en) 2017-08-22 2019-02-28 10X Genomics, Inc. Methods of producing droplets including a particle and an analyte
WO2019075409A1 (en) 2017-10-12 2019-04-18 The Regents Of The University Of California ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM)
US11745179B2 (en) 2017-10-20 2023-09-05 The Regents Of The University Of California Microfluidic systems and methods for lipoplex-mediated cell transfection
US11499127B2 (en) 2017-10-20 2022-11-15 The Regents Of The University Of California Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
CN108837778B (zh) * 2018-06-05 2020-09-15 中南大学 一种制备核壳结构载药纳米粒的方法
CN110819523B (zh) * 2018-08-13 2023-08-29 上海新微技术研发中心有限公司 一种数字pcr系统及数字pcr液滴形成方法
EP3613498A1 (en) * 2018-08-24 2020-02-26 Université de Liège Microfluidic module for co-encapsulation in droplets
CN109051819A (zh) * 2018-08-27 2018-12-21 江苏大学 一种利用空泡输运颗粒的装置与方法
CN108993342B (zh) * 2018-09-06 2024-01-23 山东清创化工有限公司 一种管式反应器内光化学反应的微混合系统及方法
CN109269762B (zh) * 2018-10-16 2020-04-28 四川大学 利用同轴平行光源观测激光空泡冲击波的实验装置
WO2020176882A1 (en) 2019-02-28 2020-09-03 10X Genomics, Inc. Devices, systems, and methods for increasing droplet formation efficiency
CN110346074A (zh) * 2019-06-13 2019-10-18 江苏大学 一种测量激光诱导空泡溃灭冲击力的装置及方法
US12186751B2 (en) 2019-06-28 2025-01-07 10X Genomics, Inc. Devices and systems incorporating acoustic ordering and methods of use thereof
US12179199B2 (en) 2019-08-09 2024-12-31 The Regents Of The University Of California Microfluidic single-cell pairing array for studying cell-cell interactions in isolated compartments
CN110743635A (zh) * 2019-10-27 2020-02-04 苏州济研生物医药科技有限公司 一种基于负压且无需流体剪切力的液滴生成装置
US12059679B2 (en) 2019-11-19 2024-08-13 10X Genomics, Inc. Methods and devices for sorting droplets and particles
CN111077487A (zh) * 2019-12-03 2020-04-28 中广核工程有限公司 便携式核仪表系统信号检测及故障定位装置
WO2021162700A1 (en) * 2020-02-14 2021-08-19 Hewlett-Packard Development Company, L.P. Droplet delivery
CN111235029B (zh) * 2020-03-09 2023-01-31 辽宁中医药大学 一种多功能微流控芯片及其制备方法与应用
CN111495450B (zh) * 2020-04-24 2021-04-06 清华大学 基于柱塞-叠片混合流的液-液-液三相流微流体芯片
US12140519B2 (en) * 2020-04-30 2024-11-12 Horiba, Ltd. Particle size distribution measurement device, particle analysis unit, program for particle size distribution measurement device, and bubble removal method
JP7737720B2 (ja) * 2020-06-16 2025-09-11 国立研究開発法人理化学研究所 ラマン散乱分光測定装置およびラマン散乱分光法
KR102491087B1 (ko) * 2020-10-12 2023-01-19 인제대학교 산학협력단 정전용량형 전극을 이용한 액적 길이 측정장치
WO2022148859A1 (en) 2021-01-11 2022-07-14 Vib Vzw Means and methods for time-resolved sampling
EP4301499A4 (en) 2021-03-05 2025-01-22 Enumerix, Inc. SYSTEMS AND METHODS FOR GENERATING DROPLETS AND PERFORMING DIGITAL ANALYSES
US20220280941A1 (en) * 2021-03-05 2022-09-08 Enumerix, Inc. Systems and methods for generating droplets and performing digital analyses
EP4351788A4 (en) 2021-06-04 2025-04-09 Enumerix, Inc. COMPOSITIONS, METHODS AND SYSTEMS FOR SINGLE-CELL ENCODING AND SEQUENCING
WO2022256612A1 (en) * 2021-06-04 2022-12-08 Enumerix, Inc. Compositions, methods, and systems for single cell barcoding and sequencing
CN113654763A (zh) * 2021-09-16 2021-11-16 中国科学院力学研究所 一种基于多种传感器的近壁面射流压力与速度测量的实验系统
GB202113798D0 (en) 2021-09-27 2021-11-10 Univ Tartu Microfluidics method
CN113967487B (zh) * 2021-10-11 2022-12-02 华中科技大学 一种喷嘴、液滴光热操控系统及其应用
US20230339226A1 (en) * 2022-04-22 2023-10-26 Bwxt Nog Technologies, Inc. Drop-on-demand electroprinter with a plunging wire-in-a-nozzle
CN115364779A (zh) * 2022-07-14 2022-11-22 南京信息工程大学 一种用于激光诱导击穿光谱的液态气溶胶发生装置
CN118201982A (zh) 2022-08-18 2024-06-14 10X基因组学有限公司 具有含氟二醇添加剂的液滴形成装置和方法
CN115364916B (zh) * 2022-08-19 2025-07-25 栎真生物科技(上海)有限公司 一种微小反应单元的生成装置及生成方法
CN115554954A (zh) * 2022-10-14 2023-01-03 中国计量大学 一种激光诱导空化溃灭驱动的气泡内液滴封装方法
US20240182849A1 (en) 2022-11-07 2024-06-06 Shennon Biotechnologies Inc. Microfluidic Devices for High Throughput Screening of Cell-Cell Interactions

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2832117B2 (ja) * 1991-11-29 1998-12-02 キヤノン株式会社 サンプル測定デバイス及びサンプル測定システム
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US6899137B2 (en) 1999-06-28 2005-05-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6524456B1 (en) * 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US20060128006A1 (en) * 1999-11-10 2006-06-15 Gerhardt Antimony L Hydrodynamic capture and release mechanisms for particle manipulation
WO2001035071A2 (en) 1999-11-10 2001-05-17 Massachusetts Institute Of Technology Cell analysis and sorting apparatus for manipulation of cells
JP2001158099A (ja) * 1999-12-02 2001-06-12 Fuji Xerox Co Ltd インクジェット記録ヘッド及びインクジェット記録装置
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
WO2002023163A1 (en) * 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
GB0105831D0 (en) 2001-03-09 2001-04-25 Toumaz Technology Ltd Method for dna sequencing utilising enzyme linked field effect transistors
US6808075B2 (en) 2002-04-17 2004-10-26 Cytonome, Inc. Method and apparatus for sorting particles
US7901939B2 (en) * 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
JP3610349B2 (ja) * 2002-08-06 2005-01-12 キヤノン株式会社 液体搬送装置
US7582482B2 (en) * 2002-09-03 2009-09-01 Dionex Corporation Continuous ion species removal device and method
CN1261767C (zh) * 2003-01-30 2006-06-28 财团法人工业技术研究院 低电压低功率热气泡薄膜式微流体驱动装置
US20050031657A1 (en) 2003-07-18 2005-02-10 Gilson Michael K. Hydrosomes; chemical vessels for laser-guided microfluidics
JP5046645B2 (ja) * 2003-09-04 2012-10-10 アリックス インコーポレイテッド 血液や細胞等の液体混合物を構成成分に分離する方法、装置およびシステム。
FR2873171B1 (fr) * 2004-07-19 2007-12-07 Centre Nat Rech Scient Cnrse Circuit microfluidique a composant actif
US7759111B2 (en) 2004-08-27 2010-07-20 The Regents Of The University Of California Cell encapsulation microfluidic device
JP2008526255A (ja) 2005-01-13 2008-07-24 マイクロニクス， インコーポレイテッド 微量流体希薄細胞検出デバイス
JP4047336B2 (ja) 2005-02-08 2008-02-13 独立行政法人科学技術振興機構 ゲル電極付セルソーターチップ
FR2884437B1 (fr) 2005-04-19 2007-07-20 Commissariat Energie Atomique Dispositif et procede microfluidique de transfert de matiere entre deux phases immiscibles.
US7918244B2 (en) * 2005-05-02 2011-04-05 Massachusetts Institute Of Technology Microfluidic bubble logic devices
JP5114702B2 (ja) * 2005-07-29 2013-01-09 国立大学法人 東京大学 両親媒性単分子膜の接触による二分子膜の形成方法およびその装置
WO2007024800A2 (en) * 2005-08-22 2007-03-01 Applera Corporation Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
FR2901717A1 (fr) * 2006-05-30 2007-12-07 Centre Nat Rech Scient Procede de traitement de gouttes dans un circuit microfluidique.
US8465706B2 (en) 2007-06-20 2013-06-18 Trustees Of Columbia University In The City Of New York On-demand microfluidic droplet or bubble generation
JP4539707B2 (ja) * 2007-10-25 2010-09-08 ソニー株式会社 微小粒子分取装置及び微小粒子分取用基板、並びに微小粒子分取方法
US8062903B2 (en) * 2008-03-03 2011-11-22 University Of Washington Droplet compartmentalization for chemical separation and on-line sampling
US20100059120A1 (en) 2008-09-11 2010-03-11 General Electric Company Microfluidic device and methods for droplet generation and manipulation
WO2010092845A1 (ja) * 2009-02-13 2010-08-19 コニカミノルタホールディングス株式会社 マイクロ流路構造体及びマイクロポンプ
WO2010110843A1 (en) 2009-03-25 2010-09-30 Eastman Kodak Company Droplet generator
JP5841937B2 (ja) 2009-06-26 2016-01-13 プレジデント アンド フェローズ オブ ハーバード カレッジ 流体注入
US9364831B2 (en) 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
WO2011023405A1 (en) 2009-08-28 2011-03-03 Georgia Tech Research Corporation Method and electro-fluidic device to produce emulsions and particle suspensions
US9625454B2 (en) * 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
US8563325B1 (en) * 2009-09-29 2013-10-22 Sandia Corporation Coaxial microreactor for particle synthesis
US20110114190A1 (en) * 2009-11-16 2011-05-19 The Hong Kong University Of Science And Technology Microfluidic droplet generation and/or manipulation with electrorheological fluid
US9772261B2 (en) * 2010-02-12 2017-09-26 Fluidion Sas Passive micro-vessel and sensor
CA2788314C (en) * 2010-02-12 2018-04-10 Dan Angelescu Passive micro-vessel and sensor
WO2012009320A2 (en) 2010-07-15 2012-01-19 Advanced Liquid Logic, Inc. Systems for and methods of promoting cell lysis in droplet actuators
US9176504B2 (en) 2011-02-11 2015-11-03 The Regents Of The University Of California High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation
EP2972405B1 (en) 2013-03-15 2020-12-23 The Regents of The University of California High-speed on demand microfluidic droplet generation and manipulation

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