JP2015508655A5 - - Google Patents
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- JP2015508655A5 JP2015508655A5 JP2014557776A JP2014557776A JP2015508655A5 JP 2015508655 A5 JP2015508655 A5 JP 2015508655A5 JP 2014557776 A JP2014557776 A JP 2014557776A JP 2014557776 A JP2014557776 A JP 2014557776A JP 2015508655 A5 JP2015508655 A5 JP 2015508655A5
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- Prior art keywords
- oligonucleotide probe
- oligonucleotide
- probe
- target
- sample
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108020005187 Oligonucleotide Probes Proteins 0.000 claims description 236
- 239000002751 oligonucleotide probe Substances 0.000 claims description 236
- 239000000523 sample Substances 0.000 claims description 141
- 108091034117 Oligonucleotide Proteins 0.000 claims description 119
- 125000003729 nucleotide group Chemical group 0.000 claims description 119
- 238000000034 method Methods 0.000 claims description 116
- 239000013615 primer Substances 0.000 claims description 115
- 239000002773 nucleotide Substances 0.000 claims description 110
- 230000000295 complement effect Effects 0.000 claims description 61
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 42
- 238000001514 detection method Methods 0.000 claims description 36
- 238000003752 polymerase chain reaction Methods 0.000 claims description 35
- 108020004707 nucleic acids Proteins 0.000 claims description 33
- 150000007523 nucleic acids Chemical class 0.000 claims description 33
- 102000039446 nucleic acids Human genes 0.000 claims description 33
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 27
- 239000011541 reaction mixture Substances 0.000 claims description 24
- 238000009396 hybridization Methods 0.000 claims description 18
- 230000037430 deletion Effects 0.000 claims description 9
- 238000012217 deletion Methods 0.000 claims description 9
- 230000037431 insertion Effects 0.000 claims description 9
- 238000003780 insertion Methods 0.000 claims description 9
- 230000035772 mutation Effects 0.000 claims description 9
- 230000005945 translocation Effects 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 101710163270 Nuclease Proteins 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 8
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
- 206010028980 Neoplasm Diseases 0.000 claims description 7
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 239000003155 DNA primer Substances 0.000 claims description 5
- 230000003321 amplification Effects 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 238000012163 sequencing technique Methods 0.000 claims description 5
- 102000003960 Ligases Human genes 0.000 claims description 4
- 108090000364 Ligases Proteins 0.000 claims description 4
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 claims description 4
- 230000001605 fetal effect Effects 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000010452 phosphate Substances 0.000 claims description 4
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 claims description 3
- 206010036790 Productive cough Diseases 0.000 claims description 3
- 210000004381 amniotic fluid Anatomy 0.000 claims description 3
- 238000001574 biopsy Methods 0.000 claims description 3
- 210000001124 body fluid Anatomy 0.000 claims description 3
- 239000010839 body fluid Substances 0.000 claims description 3
- 230000029142 excretion Effects 0.000 claims description 3
- 210000001808 exosome Anatomy 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 210000002381 plasma Anatomy 0.000 claims description 3
- 230000028327 secretion Effects 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 claims description 3
- 210000003802 sputum Anatomy 0.000 claims description 3
- 208000024794 sputum Diseases 0.000 claims description 3
- 210000001519 tissue Anatomy 0.000 claims description 3
- 210000002700 urine Anatomy 0.000 claims description 3
- 239000007787 solid Substances 0.000 description 6
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 2
- 125000002015 acyclic group Chemical group 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- -1 microtiter plates Substances 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261598343P | 2012-02-14 | 2012-02-14 | |
| US61/598,343 | 2012-02-14 | ||
| US201261605057P | 2012-02-29 | 2012-02-29 | |
| US61/605,057 | 2012-02-29 | ||
| US201261644405P | 2012-05-08 | 2012-05-08 | |
| US61/644,405 | 2012-05-08 | ||
| PCT/US2013/026180 WO2013123220A1 (en) | 2012-02-14 | 2013-02-14 | Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017075189A Division JP6479083B2 (ja) | 2012-02-14 | 2017-04-05 | 組み合わせたヌクレアーゼ反応、連結反応、およびポリメラーゼ反応を用いて核酸配列、発現、またはコピー変化を相対的に定量するための方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015508655A JP2015508655A (ja) | 2015-03-23 |
| JP2015508655A5 true JP2015508655A5 (enExample) | 2016-03-31 |
Family
ID=48984705
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557776A Pending JP2015508655A (ja) | 2012-02-14 | 2013-02-14 | 組み合わせたヌクレアーゼ反応、連結反応、およびポリメラーゼ反応を用いて核酸配列、発現、またはコピー変化を相対的に定量するための方法 |
| JP2017075189A Active JP6479083B2 (ja) | 2012-02-14 | 2017-04-05 | 組み合わせたヌクレアーゼ反応、連結反応、およびポリメラーゼ反応を用いて核酸配列、発現、またはコピー変化を相対的に定量するための方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017075189A Active JP6479083B2 (ja) | 2012-02-14 | 2017-04-05 | 組み合わせたヌクレアーゼ反応、連結反応、およびポリメラーゼ反応を用いて核酸配列、発現、またはコピー変化を相対的に定量するための方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US9598728B2 (enExample) |
| EP (1) | EP2814986B1 (enExample) |
| JP (2) | JP2015508655A (enExample) |
| CN (1) | CN104204228A (enExample) |
| AU (2) | AU2013221480B2 (enExample) |
| CA (1) | CA2863257C (enExample) |
| ES (1) | ES2655509T3 (enExample) |
| IL (1) | IL233908A0 (enExample) |
| SG (1) | SG11201404899VA (enExample) |
| WO (1) | WO2013123220A1 (enExample) |
Families Citing this family (106)
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| US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
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| PT2556171E (pt) | 2010-04-05 | 2015-12-21 | Prognosys Biosciences Inc | Ensaios biológicos codificados espacialmente |
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