JP2015501647A - Active small molecule glue mixture, preparation method and use thereof - Google Patents

Abstract

Active small molecule glue mixture, and its preparation and use. The active small molecule glue mixture is prepared by enzymatic hydrolysis of glue liquor to which no auxiliary material has been added, using a complex protease containing proline protease. The active small molecule glue mixture has a weight average molecular weight in the range of 580 Da to 1300 Da, a peptide portion distributed in 200 Da to 3000 Da, a high dissolution rate in cold water, a low free amino acid content, and the activity The small molecule glue mixture may be used in the production of small molecule peptide foods and insurance foods for glue. [Selection] Figure 1

Description

  The present invention relates to an active small molecule mixture, and a preparation method and use thereof. Specifically, the present invention relates to an active small molecule peptide mixture obtained by enzymatic hydrolysis of glue liquid, and an application method and use thereof. The present invention belongs to the field of food biotechnology.

  Ag is an effective drug that supplements the blood and nurtures it. As early as in the later Han Dynasty, Akagi was described as “elegant” in the prestigious drug book “Shinnohonhon Sukei”, and “If you take it for a long time, you will feel better and feel lighter”. has been written. The characteristics of Aji are “sweet” and “flat”, and its main function is to supplement the blood, nourish the shade, improve the dry state, and stop bleeding. Ag is skin yellowing due to lack of blood, dizziness, palpitation, muscle atrophy and weakness, emotional hypersensitivity, insomnia, internal movement of the wind, cough due to dryness of the lungs, labor cough, hemoptysis, vomiting and hematuria, bloody stool, imminent abortion, etc. Used to treat. According to the 2010 Chinese Pharmacy, Aji is a solid gelatin made by boiling dry or raw skin of equine donkeys and then concentrating the decoction. However, the molecular weight of collagen protein is very large (tens of thousands to hundreds of thousands), and patients with weak spleen and stomach cannot be absorbed, and as a result, the function of glue is not fully exhibited. At the same time, the number of donkeys has been bred in recent years, which inevitably leads to a shortage of donkey skin supply and increases in the price of glue. Due to the growing supply of donkey skin material, the market demand cannot be met, but small molecule glue obtained by enzymatic hydrolysis of glue fluid can greatly increase the bioavailability of glue. It can be a good way to close the gap between supply and demand.

  In recent years, some researches on the enzymatic degradation of glue have made some progress: (1) Chinese Patent Application No. 1237421A “Method of preparing liquid glue by enzymatic degradation”, (2) Chinese Patent Application Publication No. 1807653A “Active collagen collagen Numerous patents have been published, including "Peptide Products", (3) Chinese Patent Application No. 1012669090A "Method for preparing low-molecular weight glue hydrolyzate". All three inventions report the production of collagen peptides by protease digestion of glue. Based on the existing technology, the present invention proposes to enzymatically digest Ag with proline protease for the first time, utilizing the characteristically high proline and hydroxyproline content in Ag, and as a result, the past patents The hydrolysis effect is significantly higher than that reported in. In the three patents mentioned above, the molecular weight of most products was distributed in the range of less than 5000 Da and even less than 10,000 Da. In addition, in any of the above patents, accurate measurement results of molecular weight distribution and free amino acid content are not reported, but in the present invention, the peptide molecular weight is in the range of 200 to 3000 Da, mainly in the range of 200 to 1000 Da. Distributed. In both Chinese Patent Application Publication No. 1237421A and Chinese Patent Application Publication No. 1807653A, the lump of glue is used together with the auxiliary material added as a digestion raw material, but in the present invention, the process is simplified and the cost is reduced. In order to obtain a product having good solubility and high bioavailability, glue solution is used as a raw material for enzymatic degradation, and the DPPH radical and ABTS radical elimination rates are close to 100%.

  In many reports in the literature (Adivi AS S. America Nutrition, 1984, 25: 1114-1122, DBA Silk Gut, 1974, 15: 494-501), peptides with molecular weights in the range of 200-1000 Da are Absorption and efficacy have been shown to be superior to size peptides and free amino acids. The present invention proposes the use of glue liquid without auxiliary materials as raw materials in order to accurately determine the content of free amino acids in the product and the molecular weight distribution of the product, and the small peptide contained in the product is Since it is generally distributed in the range of 200 to 1000 Da, the absorbency and efficacy are high.

  In order to solve the problems in existing techniques, one of the objects of the present invention is to provide active small molecule glue mixtures.

  The second object of the present invention is to provide a method for preparing active small molecule glue mixtures.

  The third object of the present invention is to provide application of the active small molecule glue mixture to the production of small molecule peptide foods and health foods.

  In order to achieve the above object, the present invention employs the following technical means.

  An active small molecule glue mixture proposed in the present invention, wherein the product has a weight average molecular weight of 580 to 1300 Da, and peptides are distributed in 200 to 3000 Da, mainly 200 to 1000 Da.

  In the present invention, an active small molecule glue mixture is prepared by enzymatic hydrolysis of glue liquid using a complex protease. Preferably, the complex protease includes papain and proline protease, and the complex proteinase further includes one, a plurality, or all of a protease selected from the group consisting of bromelain protease, neutral protease, pepsin, and flavorzyme. .

  In the present invention, the active small molecule glue mixture can be processed into a powder or liquid preparation.

  The characteristics of the active small molecule glue mixture proposed in the present invention are as follows: (1) it can be processed into a powder or liquid preparation for oral administration; (2) it has good solubility and a high dissolution rate in cold water; 3) Small peptides with low free amino acid content (only 4%) and molecular weights in the range of 200-1000 Da accounted for 81% of the total, 1000-3000 Da peptides accounted for 14% (see FIG. 1), (4) Regarding bioavailability, the bioavailability of small molecule glue is 3.5 times that of normal glue, and 2.2 of biodigested glue. (5) In terms of antioxidant effect, small molecule glue has an excellent effect of removing radicals including DPPH and ABTS, and has an erasure rate of 10 mg / ml. Can be close to 100% A (see FIGS. 3 and 4).

The present invention is a method of making an active small molecule glue mixture comprising:
(1) Cool the glue solution not containing auxiliary materials to 30-55 ° C. at room temperature, adjust the pH to 5-8, add complex protease to this solution, and perform the hydrolysis reaction for 0.5-2 hours. To obtain a hydrolyzate of agar liquid,
(2) A step of obtaining an inactivated decoction by incubating the hydrolyzate of the glue liquid obtained in step (1) for 10 to 30 minutes to inactivate the enzyme,
(3) obtaining a liquid formulation of active small molecule glue mixture by centrifuging the inactivated decoction to remove impurities;
A method is also provided.

  The method for preparing the active small molecule glue mixture proposed in the present invention may further include the step of spray-drying the liquid preparation to produce a powder formulation of the active small molecule glue mixture.

  In the method proposed in the present invention, the auxiliary material includes yellow liquor, rock sugar, and soybean oil, and the gelatin liquid not including the auxiliary material is a glue liquid that does not contain yellow wine, rock sugar, or soybean oil.

  In the embodiment of the present invention, the concentration of the glue liquid in the step (1) is 10% to 50% (w / w).

  In an embodiment of the present invention, the complex protease includes papain and proline protease, and the complex proteinase further includes one or more proteases selected from the group consisting of bromelain protease, neutral protease, pepsin, and flavorzyme. Or all.

  In the embodiment of the present invention in which the amount of the complex protease added in the step (1) is 5% by weight to 3% by weight (w / w) of the glue solution, the order of addition of the complex protease is constant for the enzymes described above. Either at intervals or at the initial stage of the hydrolysis reaction, the above enzymes are added simultaneously.

  In certain embodiments of the invention, by weight, each addition ratio of complex proteases is papain: proline protease = 2: 1, or papain: proline protease: bromelain, neutral protease, pepsin, or flavorzyme. Any one = 5: 2: 1. Other ratios are not described in detail. A person skilled in the art can make a wide variety of choices based on the actual situation at the time of application without any creative work.

  The present invention further provides the application of active small molecule glue mixtures to the preparation of small molecule peptide foods and health foods. Small molecule active peptide mixture improves absorption, shows very good solubility and very good anti-oxidant effect, so foods in powder or liquid formulation and health with anti-oxidation and aging delay Useful for food development.

  The advantages of the present invention over existing techniques are as follows.

  1: The proposed method uses a glue liquid that does not contain any auxiliary material as a raw material, thereby simplifying the process, saving energy, reducing costs, highly efficient hydrolysis, and the structure and properties of the spray-dried product. It will ensure stability.

  2: The proposed method removes fats and impurities in the product by centrifugation, so that the product is highly soluble, and the product helps the development of related foods and health foods To.

  3: In the proposed method, the powder raw material is obtained using spray drying to ensure low storage costs and ease of development of capsules and granule products.

  4: The proposed method advances product testing techniques so that the free amino acid content and peptide distribution can be accurately determined.

  5: In the proposed method, the enzymatic degradation technology has progressed, the molecular weight of peptides in the product is mainly distributed in the range of 200-1000 Da, this molecular weight range is lower than that obtained by published processes This ensures higher absorbency and functional effects.

  6: The production technique proposed in the present invention is advanced and practical, and a high-molecular-weight mixture with high yield is secured from the glue. For example, using 1 L of 40% glue solution as a raw material, the powder product obtained after the enzymatic decomposition and the subsequent process was about 360 g, and the yield was 90%.

  7: Preliminary determination of the bioavailability and antioxidant effect of the obtained substance showed very good results.

Molecular weight distribution of active small molecule glue mixtures measured using high performance gel chromatography in combination with amino acid analysis techniques. Results of comparing bioavailability by comparing the hydroxylproline content in the blood of mice after giving the product according to the invention and the product by different techniques, Bio-digested glue is a normal untreated glue sample, which is obtained by enzymatic digestion of glue using pepsin and intestinal trypsin, mimicking digestion in the human gastrointestinal tract. An active small molecule glue mixture obtained by using the method proposed in the present invention. The active small molecule glue mixture obtained according to the present invention has the effect of removing DPPH radicals. The active small molecule glue mixture obtained according to the present invention has the effect of removing ABTS radicals.

  In the following, the present invention will be further explained by embodiments. The advantages and features of the invention will become apparent from this description. It should be understood that these embodiments are illustrative only and do not in any way limit the scope of protection of the present invention. Those skilled in the art will recognize that the details and shape of the invention can be modified or replaced without departing from the spirit and scope of the invention, and all such modifications and substitutions are Within protection range.

  The sources of experimental material relevant to the embodiments of the present invention are as follows.

  1: Astringent fluid without yellow liquor, rock sugar, and soybean oil was obtained from Shandong Dong-E Donkey-hide gelatin Co. , Ltd., Ltd. Purchased from.

2: Various enzymes Flavorzyme, neutral protease, and bromelain were obtained from Pangbo Bioengineering Co. , Ltd., Ltd. Papain and pepsin were purchased from Keyuan Industrial Co., Ltd., Nanning, China. , Ltd., Ltd. Proline protease was purchased from Shanhai Yuanda Business Ltd. Purchased from.

  3: ABTS radical and DPPH radical are available from Sigma Aldrich Co. , Ltd., Ltd. Purchased from.

Example 1
1 L of glue liquid (40% (w / w)) without added auxiliary material was cooled to 35 ° C. at room temperature, and its pH was adjusted to 6.5. To this solution, 5% (w / w) complex protease (papain: proline protease = 2: 1 (w / w)) was added, a hydrolysis reaction was carried out for 2 hours, and this solution was heated to boil, Boiled for a minute to inactivate the above enzyme and then centrifuged to remove insoluble impurities and fat to obtain a liquid preparation of active small molecule glue mixture. When the molecular weight of this product was determined, most of the peptides ranged from 200 to 3000 Da, accounting for 83% of the total.

Example 2
1 L of glue liquid (40% (w / w)) without added auxiliary material was cooled to 35 ° C. at room temperature, and its pH was adjusted to 6.5. To this solution, 5% (w / w) complex protease (papain: proline protease = 2: 1 (w / w)) was added, the hydrolysis reaction was performed for 2 hours, and this solution was boiled for 20 minutes, The enzyme was inactivated and then centrifuged to remove insoluble impurities and fat, and finally the liquid was spray dried to give 360 g of powdered active small molecule glue mixture. When the molecular weight of this product was determined, most of the peptides ranged from 200 to 3000 Da, accounting for 83% of the total.

Example 3
1 L of glue liquid (40% (w / w)) without added auxiliary material was cooled to 40 ° C. at room temperature, and its pH was adjusted to 5.5. 1% (w / w) complex protease (papain: proline protease: pepsin = 5: 2: 1 (w / w)) was added to this solution, and the hydrolysis reaction was carried out for 2 hours, and this solution was boiled for 20 minutes. Subsequently, the enzyme was inactivated, and then centrifuged to remove insoluble impurities and fat. Finally, the liquid was spray-dried to obtain 360 g of powdered active small molecule glue mixture. When the molecular weight of this product was determined, the majority of peptides ranged from 200 to 3000 Da, accounting for 86% of the total.

Example 4
1 L of glue liquid (10% (w / w)) without added auxiliary material was cooled to 35 ° C. at room temperature, and its pH was adjusted to 6. 2% (w / w) of complex protease (papain: proline protease: neutral protease: pepsin: flavorzyme = 6: 3: 1: 1: 1 (w / w)) was added to this solution to conduct hydrolysis reaction. Incubate for 2 hours and boil this solution for 20 minutes to inactivate the enzymes, then centrifuge to remove insoluble impurities and fat, and finally spray dry the liquid to give 360 g of powdered small active molecule An adhesive mixture was obtained. When the molecular weight of this product was determined, most of the peptides ranged from 200 to 3000 Da, accounting for 75% of the total.

Example 5
1 L of glue liquid (40% (w / w)) without added auxiliary material was cooled to 50 ° C. at room temperature, and its pH was adjusted to 6.5. 3% (w / w) complex protease (papain: proline protease: pepsin: neutral protease: flavorzyme = 6: 2: 2: 1: 1 (w / w)) was added to this solution, and the hydrolysis reaction was performed. Incubate for 2 hours and boil this solution for 20 minutes to inactivate the enzymes, then centrifuge to remove insoluble impurities and fat, and finally spray dry the liquid to give 360 g of powdered small active molecule An adhesive mixture was obtained. When the molecular weight of this product was determined, most of the peptides ranged from 200 to 3000 Da, accounting for 95% of the total.

Example 6
1 L of glue liquid (40% (w / w)) without added auxiliary material was cooled to 40 ° C. at room temperature, and its pH was adjusted to 6.5. 3% (w / w) complex protease (papain: proline protease: pepsin: neutral protease: flavorzyme = 6: 2: 2: 1: 1 (w / w)) was added to this solution, and the hydrolysis reaction was performed. Incubate for 0.5 hour, heat to boil, continue to boil for 20 minutes to inactivate the enzyme, centrifuge to remove insoluble impurities and fat, and finally spray dry the liquid. 360 g of powdered active small molecule glue mixture was obtained. When the molecular weight of this product was determined, most of the peptides were 200-3000 Da, accounting for 83% of the total.

Example 7
1 L of glue liquid (40% (w / w)) without added auxiliary material was cooled to 40 ° C. at room temperature, and its pH was adjusted to 6.5. 5% (w / w) complex protease (papain: proline protease: pepsin: neutral protease: flavorzyme = 6: 2: 2: 1: 1 (w / w)) was added to this solution, and the hydrolysis reaction was performed. Heated to boil this liquid for 2 hours, boiled for 20 minutes to inactivate the above enzymes, then centrifuged to remove insoluble impurities and fat, and finally the liquid was spray dried to 360 g A powdery active small molecule glue mixture was obtained. When the molecular weight of this product was determined, most of the peptides were 200-3000 Da, accounting for 71% of the total.

Example 8
1 L of glue liquid (40% (w / w)) without added auxiliary material was cooled to 40 ° C. at room temperature, and its pH was adjusted to 6.5. To this solution, 1.5% (w / w) of complex protease (papain: proline protease = 2: 1 (w / w)) was added and the hydrolysis reaction was carried out for 0.5 hour, and then 5% (w / w). by adding w) pepsin and continuing the hydrolysis for 0.5 h followed by 5% (w / w) complex protease (neutral protease: flavorzyme = 1: 1 (w / w)) Hydrolysis is continued for 1 hour, heated to boil this liquid, boiled for 20 minutes to inactivate the above enzymes, then centrifuged to remove insoluble impurities and fat, and finally the liquid is spray dried As a result, 360 g of powdered active small molecule glue mixture was obtained. When the molecular weight of this product was determined, most of the peptides ranged from 200 to 3000 Da, accounting for 87% of the total.

Example 9
1 L of glue liquid (40% (w / w)) without added auxiliary material was cooled to 50 ° C. at room temperature, and its pH was adjusted to 6.5. 3% (w / w) of complex protease (papain: pepsin: neutral protease: flavorzyme: bromelain = 6: 2: 2: 1: 1 (w / w)) was added to this solution, and the hydrolysis reaction was carried out to 2 Heat for simmering this liquid, continue simmering for 20 minutes to inactivate the above enzyme, then centrifuge to remove insoluble impurities and fat, and finally spray dry this liquid to 360 g A powdery active small molecule glue mixture was obtained. When the molecular weight of this product was determined, the majority of peptides ranged from 200 to 3000 Da, accounting for 65% of the total.

  A powdered active small molecule glue mixture prepared according to Example 5 of an embodiment of the present invention was used in the following test analysis.

Example 10: Testing the distribution of peptides in active small molecule glue mixtures 1.1: Testing using high speed gel chromatography Test equipment:
Liquid system: Agilent 1100, efficient gel column: TSK-G2000 SKwl (300 mm × 7.8 mm, average pore diameter 500 mm)
Test procedure:
A 1 ml hydrolyzed sample was weighed and centrifuged at 10,000 g for 2 minutes, and the supernatant was filtered through a 0.45 μm disposable filter vial before introducing the sample into the chromatograph. The column temperature was 27 ° C., the mobile phase was 0.05 mol / L phosphate buffer (pH 7.2), the flow rate was 0.5 ml / min, the detection wavelength was 280 nm, and the introduction volume was 20 μl. It was.

1.2: Test for free amino acid content Test equipment:
MS: 3200 Q Trap, LC system, Shimadzu UFLC system, column: AB Sceix dedicated to amino acid analysis, guard column: C18 (4.0 × 3.0 mm, 5 mm), Phenomenex Co.
procedure:
Add 5 μL of precipitant to a uniformly mixed 20 μL liquid sample, mix uniformly, centrifuge at 10,000 g for 2 minutes, add 20 μL of labeling buffer to 5 μL of supernatant, and mix until uniform. Add 5 μL of supernatant with 2.5 μL of labeling reagent, mix until uniform, react at room temperature for 30 minutes, add 2.5 μL of hydroxylamine after reaction, mix until uniform, 40 ° C. Dry by blowing with nitrogen, reconstitute by adding 16 μL of internal standard solution, mix until uniform, weigh 16 μL into a sample vial, introduce 5 μL into the chromatograph and adjust the amino acid concentration. It was measured.

1.3: Results The results are shown in FIG. As can be seen from FIG. 1, the sample contains a high content (81%) of peptides with a molecular weight in the range of 200-1000 Da, the portion of the 1000-3000 Da peptide accounts for 14% of the total and contains free amino acids. The amount was very small, only 4%. The weight average molecular weight was 765 Da. As described in the background section of the present invention, peptides having a molecular weight of 200-1000 Da are more absorbable and more effective than other sized peptides and free amino acids. In comparison, the product according to the invention can be considered excellent in terms of absorption rate and functional efficacy.

Example 11 Bioavailability Test Materials:
Normal glue: Normal untreated glue sample Biologically digested glue: A model of digestion of human glue in the gastrointestinal tract, using pepsin and intestinal trypsin. Small molecule glue: In the present invention Active small molecule glue mixture obtained by using the proposed method Twenty-four healthy mice (12 males, 12 females, 200-250 g body weight) were randomly divided into groups A, B, and C. The drug was administered after fasting for 12 hours. The next morning, group A was given a normal glue solution, group B was biodigested glue solution, and group C was given a small molecule glue intragastrically (each sample was 1 mg / body weight 10 g), and feeding resumed 4 hours after administration. 0 hours, 0.25 hours, 0.5 hours, 1 hour, 2 hours, 3 hours, 4 hours, 6 hours, 8 hours, 10 hours, 12 hours after administration Orbital blood samples were collected after 18 and 24 hours. After pretreatment, blood samples were analyzed using amino acid analysis to obtain blood hydroxylproline content and to standardize sample bioavailability (hydroxylproline is a characteristic amino acid of collagen).

  The results are shown in FIG. 2. From FIG. 2, the bioavailability of the active small molecule glue mixture proposed in the present invention is 3.5 times that of ordinary glue, 2 of the product obtained from biodigestion. It turns out that it is 2 times. These data indicated that the active small molecule glue mixture proposed in the present invention has small molecule characteristics and high absorption rate.

Example 12 Test on Antioxidant Effect 1.1: Investigation on Effect of Removing DPPH Radical Procedure Add 1.5 mL sample solution and 1.5 mL DPPH solution to a stoppered test tube and shake for 30 minutes under light shielding. The absorbance at 517 nm (A _S ) was measured, and at the same time, the absorbance (A _C ) of a mixture of 1.5 mL of 0.1 mmol / L DPPH solution and 1.5 mL of ethanol, and sample solution 1 The absorbance (A _B ) of a mixture of 5 mL and 1.5 mL of ethanol was determined. The erasure rate was calculated according to the following formula.

Wherein the absorbance of the reactant between A _S sample solution, A _C is the absorbance of the mixture of DPPH solution and ethanol, A _B is the absorbance of the mixture of the sample solution and ethanol.

1.2: Investigation on the effect of removing ABTS radicals Test procedure After reacting 88 μL of potassium persulfate solution with 5 mL of ABTS solution for 16 hours under shading, until the absorbance becomes 0.7 ± 0.002. The reaction mass is diluted with ethanol. 200 μL (2 mL) of the ABTS reaction solution was reacted with 100 μL (1 mL) of the sample solution for 10 minutes in a 96-well plate, and the absorbance at 734 nm was measured. The erasure rate was calculated according to the following formula.

In the formula, A ₀ is the absorbance of the ABTS ^{· +} solution, and A is the absorbance of the ABTS ^{· +} solution after adding the sample solution.

1.3: Results The results are shown in FIG. 3 and FIG. As can be seen from FIGS. 3 and 4, the small molecules of various concentrations of small molecule glue solution all show the effect of removing ABTS radicals and DPPH radicals, and the small molecule glue solution with a concentration of 10 mg / ml It also showed an erasing rate close to 100% for radicals, indicating that the product according to the invention has a certain antioxidant effect.

Claims (11)

  An active small molecule glue mixture, wherein the mixture has a weight average molecular weight of 580 to 1300 Da, a peptide molecular weight of 200 to 3000 Da, mainly distributed to 200 to 1000 Da An active small molecule glue mixture in which the mixture is prepared by hydrolysis and the complex protease comprises papain and proline protease.   The active small molecule glue mixture according to claim 1, wherein the complex protease further comprises one, a plurality, or all of a protease selected from the group consisting of bromelain protease, neutral protease, pepsin, and flavorzyme.   The active small molecule glue mixture according to claim 1 or 2, wherein the preparation of the mixture is a powder or a liquid. A method of making the active small molecule glue mixture according to any one of claims 1, 2, or 3.
(1) Cool the glue solution not containing auxiliary materials to 30-55 ° C. at room temperature, adjust the pH to 5-8, add complex protease to this solution, and perform the hydrolysis reaction for 0.5-2 hours. By the step of obtaining a hydrolyzate of glue liquid, wherein the complex protease comprises papain and proline protease;
(2) A step of obtaining an inactivated decoction by inactivating the enzyme by continuing to boil the hydrolyzate of the glue liquid obtained in step (1) for 10 to 30 minutes;
(3) obtaining a liquid formulation of an active small molecule glue mixture by centrifuging the inactivated decoction to remove impurities;
Including methods.   5. The method of claim 4, wherein the resulting liquid formulation of active small molecule glue mixture is further processed by spray drying to a powder formulation.   The method of claim 4, wherein the auxiliary material comprises yellow liquor, rock sugar, and soybean oil.   The method according to claim 4, wherein the concentration of the glue liquid in step (1) is 10% to 50%.   5. The method of claim 4, wherein the complex protease further comprises one, a plurality, or all of a protease selected from the group consisting of bromelain protease, neutral protease, pepsin, and flavorzyme.   The weight of the complex protease to be added is 5% to 3% of the weight of the glue solution, and the order of adding the complex protease is to add a plurality of enzymes in order at regular intervals, or at the initial stage of the hydrolysis reaction. The method according to claim 4, wherein the enzymes are added simultaneously.   The weight ratio of the constituent enzymes in the complex protease to be added is papain: proline protease = 2: 1, or any one of papain: proline protease: bromelain, neutral protease, pepsin, or flavorzyme = 5: 2: 1. The method of claim 4, wherein   Use of the active small molecule glue mixture according to any one of claims 1 to 3 in the preparation of small molecule peptide foods or health foods.

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