CN105767451A - Extraction method of honey solid small molecular peptides - Google Patents
Extraction method of honey solid small molecular peptides Download PDFInfo
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- CN105767451A CN105767451A CN201610190973.5A CN201610190973A CN105767451A CN 105767451 A CN105767451 A CN 105767451A CN 201610190973 A CN201610190973 A CN 201610190973A CN 105767451 A CN105767451 A CN 105767451A
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- mel
- molecular peptides
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- extracting method
- small molecular
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 38
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 36
- 235000012907 honey Nutrition 0.000 title claims abstract description 19
- 238000000605 extraction Methods 0.000 title abstract description 8
- 239000007787 solid Substances 0.000 title abstract 3
- 239000000243 solution Substances 0.000 claims abstract description 40
- 102000004190 Enzymes Human genes 0.000 claims abstract description 23
- 108090000790 Enzymes Proteins 0.000 claims abstract description 23
- 229940088598 enzyme Drugs 0.000 claims abstract description 22
- 239000000706 filtrate Substances 0.000 claims abstract description 19
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 16
- 230000007935 neutral effect Effects 0.000 claims abstract description 13
- 102000057297 Pepsin A Human genes 0.000 claims abstract description 11
- 108090000284 Pepsin A Proteins 0.000 claims abstract description 11
- 239000012670 alkaline solution Substances 0.000 claims abstract description 11
- 229940111202 pepsin Drugs 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 11
- 108090000145 Bacillolysin Proteins 0.000 claims abstract description 10
- 108091005507 Neutral proteases Proteins 0.000 claims abstract description 10
- 102000035092 Neutral proteases Human genes 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 19
- 230000000694 effects Effects 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 15
- 108091005658 Basic proteases Proteins 0.000 claims description 13
- 239000002253 acid Substances 0.000 claims description 10
- 229910052799 carbon Inorganic materials 0.000 claims description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 abstract description 4
- 239000004365 Protease Substances 0.000 abstract description 4
- 108010016626 Dipeptides Proteins 0.000 abstract description 3
- 150000003384 small molecules Chemical group 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 239000003929 acidic solution Substances 0.000 abstract 3
- 238000010438 heat treatment Methods 0.000 abstract 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 239000003513 alkali Substances 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
- 239000011874 heated mixture Substances 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 108010038807 Oligopeptides Proteins 0.000 description 3
- 102000015636 Oligopeptides Human genes 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 241000256836 Apis Species 0.000 description 1
- 241000256844 Apis mellifera Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000004206 stomach function Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
The present invention relates to an extraction method, in particular to an extraction method of honey solid small molecular peptides. The extraction method comprises the following steps: mixing and stirring honey with water, heating the mixture, cooling the heated mixture, adding alkali protease into the cooled mixture, and adjusting pH to obtain alkaline solution; adding neutral protease into the alkaline solution, and adjusting the pH to obtain neutral solution; adding pepsin into the neutral solution, and adjusting the pH to obtain acidic solution; heating the acidic solution, and filtering the acidic solution to obtain filtrate; and adding activated carbon into the filtrate to be decolorized, and performing filtration, concentration and drying to obtain the honey solid small molecular peptide powder. Beneficial effects of the extraction method are that: the extraction method uses compound biological enzyme enzymatic hydrolysis technology, molecular weights of the obtained small molecular peptides are mainly distributed in 180-480 dalton, most of the obtained small molecular peptides are dipeptides, tripeptides, tetrapeptides, etc., which belong to small molecule structure, and the obtained small molecular peptides have the characteristic that the small molecular peptides can easily be absorbed by the human body.
Description
Technical field
The present invention relates to a kind of extracting method, be specifically related to the extracting method of a kind of Mel entity small-molecular peptides.
Background technology
Nectar honey of brew in Nidus Vespae that Mel is insecticide Apis to be adopted from the spending of flowering plant.Mel
Composition in addition to glucose, fructose possibly together with various vitamin, mineral, amino acid and protein.Honeybee
Honey is a kind of nutritious food, also has certain curative effect to some chronic disease, and containing other multiple people
The trace element that body is indispensable.
General 2-6 aminoacid can form oligopeptide, the molecular weight section of oligopeptide typically below 1000 dalton,
And small-molecular peptides is Ultra-low molecular weight oligopeptide, only by the high activity peptide of 2-4 Amino acid profile, molecular weight one
As 180-480 dalton.It is not required to digestion at human body, can directly absorb, be not required to when there is absorption consume
Body energy, will not increase human intestines and stomach's function burden, can preferential absorption and with self-energy promote human body
The advantages such as absorption.
Along with the development of human society, health is increasingly paid close attention to by people, and the value of small-molecular peptides have also been obtained
The attention of people, but but it is rarely mentioned about the extraction of entity small-molecular peptides in Mel.
Summary of the invention
In order to solve the problems referred to above that prior art exists, the invention provides a kind of Mel entity small-molecular peptides
Extracting method.
The technical solution adopted in the present invention is:
The extracting method of a kind of Mel entity small-molecular peptides, comprises the steps:
Step 1, Mel is mixed with water, is heated with stirring to 50-80 DEG C, be incubated 15min, then cool to 40 DEG C,
Adding alkaline protease, regulate pH to 8-9,40 DEG C are incubated 2-3 hour, obtain alkaline solution；
Step 2, in described alkaline solution add neutral protease, regulate pH to 6-7,40 DEG C insulation 2-3
Hour, obtain neutral solution；
Step 3, addition pepsin, regulation pH to 2.5-3.7,40 DEG C of insulations in described neutral solution
2-3 hour, obtain acid solution；
Step 4, described acid solution is heated, be warming up to 80-90 DEG C, be incubated 10-20min, be filtrated to get
Filtrate；
Step 5, in described filtrate, add activated carbon decolour, afterwards through filtering, concentrating, dry,
Obtain Mel entity small-molecular peptides powder.
Further, in step 1, it is that 1:8-13 mix with water according to mass ratio by described Mel.Step 2
In, adding the alkaline protease that enzyme activity is 500,000 u/g, the addition of described alkaline protease is Mel matter
The 1% of amount.Described alkaline protease refers in the basic conditions can the enzyme of aminosal peptide bond.
Further, in step 2, add the neutral protease that enzyme activity is 150,000 u/g, described neutral egg
Addition is honey quality the 1% of white enzyme.Described neutral protease is by the fermented extraction of bacillus subtilis
Obtained by, belong to a kind of restriction endonuclease, can be used for various proteolysis and process.In step 3, add enzyme and live
Power is the pepsin of 50,000 u/g, and described pepsic addition is the 1% of honey quality.In step 1
The NaOH solution regulation pH using mass fraction to be 10%；Using mass fraction in step 2 is 10%
HC1 solution regulation pH；The HC1 solution regulation pH using mass fraction to be 10% in step 3.
Further, step 4 use 300-400 mesh gauze or filter paper filter.
Further, in step 5, in filtrate, add the activated carbon of filtrate weight 1-2%, at 60-70 DEG C
Stirring decolouring 20-30min；Filter with diatomite filter afterwards, obtain clear liquid；And
55 DEG C of 15-20% that described clear liquid vacuum is concentrated into primary liquid weight；Finally it is spray-dried
Or lyophilization obtains described Mel entity small-molecular peptides powder.
The invention have the benefit that the extracting method of the Mel entity small-molecular peptides that the present invention provides utilizes multiple
Symphysis thing enzyme resolving tech, the small-molecular peptides molecular weight obtained is mainly distributed between 100-1000D, mostly is
The small-molecular peptides such as dipeptides, tripeptides, tetrapeptide, belong to small molecule structure, having the utilization that is easily absorbed by the body
Feature.
Additionally, Mel can also be improved looks, facilitating digestion, the small-molecular peptides in Mel can improve human body
Immunity, protection cardiovascular, and can quickly be absorbed by the body, there is the effectiveness that well keeps healthy.
Detailed description of the invention
Embodiment 1
The extracting method of a kind of Mel entity small-molecular peptides, comprises the steps:
Step 1, it is that 1:8 mix with water according to mass ratio by Mel, is heated with stirring to 50 DEG C, is incubated 15min,
Cool to 40 DEG C again, add the alkaline protease that enzyme activity is 500,000 u/g, the interpolation of described alkaline protease
Amount is the 1% of honey quality；The NaOH solution regulation pH to 8 using mass fraction to be 10% afterwards, 40 DEG C
It is incubated 2 hours, obtains alkaline solution；
Step 2, to add enzyme activity in described alkaline solution be the neutral protease of 150,000 u/g, described in
Addition is honey quality the 1% of property protease；The HC1 solution using mass fraction to be 10% afterwards is adjusted
Joint pH to 6,40 DEG C are incubated 2 hours, obtain neutral solution；
Step 3, to add enzyme activity in described neutral solution be the pepsin of 50,000 u/g, described pepsin
The addition of enzyme is the 1% of honey quality；The HC1 solution regulation pH using mass fraction to be 10% afterwards
To 2.5,40 DEG C are incubated 2 hours, obtain acid solution；
Step 4, described acid solution is heated, be warming up to 80 DEG C, be incubated 10min, use 300 mesh yarns
Cloth filters, and obtains filtrate；
Step 5, in described filtrate add filtrate weight 1% activated carbon, 60 DEG C stirring decolouring 20min；
Filter with diatomite filter afterwards, obtain clear liquid；And at 55 DEG C by described clear
Liquid vacuum is concentrated into the 15% of primary liquid weight；Finally carry out spray drying and obtain little point of described Mel entity
Sub-peptide powder.
Embodiment 2
The extracting method of a kind of Mel entity small-molecular peptides, comprises the steps:
Step 1, it is that 1:13 mix with water according to mass ratio by Mel, is heated with stirring to 80 DEG C, is incubated 15min,
Cool to 40 DEG C again, add the alkaline protease that enzyme activity is 500,000 u/g, the interpolation of described alkaline protease
Amount is the 1% of honey quality；The NaOH solution regulation pH to 9 using mass fraction to be 10% afterwards, 40 DEG C
It is incubated 3 hours, obtains alkaline solution；
Step 2, to add enzyme activity in described alkaline solution be the neutral protease of 150,000 u/g, described in
Addition is honey quality the 1% of property protease；The HC1 solution using mass fraction to be 10% afterwards is adjusted
Joint pH to 7,40 DEG C are incubated 3 hours, obtain neutral solution；
Step 3, to add enzyme activity in described neutral solution be the pepsin of 50,000 u/g, described pepsin
The addition of enzyme is the 1% of honey quality；The HC1 solution regulation pH using mass fraction to be 10% afterwards
To 3.7,40 DEG C are incubated 3 hours, obtain acid solution；
Step 4, described acid solution is heated, be warming up to 90 DEG C, be incubated 20min, use 400 mesh yarns
Cloth filters, and obtains filtrate；
Step 5, in described filtrate add filtrate weight 2% activated carbon, 70 DEG C stirring decolouring 30min；
Filter with diatomite filter afterwards, obtain clear liquid；And at 55 DEG C by described clear
Liquid vacuum is concentrated into the 20% of primary liquid weight；Finally carry out spray drying and obtain little point of described Mel entity
Sub-peptide powder.
Embodiment 3
The extracting method of a kind of Mel entity small-molecular peptides, comprises the steps:
Step 1, it is that 1:10 mix with water according to mass ratio by Mel, is heated with stirring to 60 DEG C, is incubated 15min,
Cool to 40 DEG C again, add the alkaline protease that enzyme activity is 500,000 u/g, the interpolation of described alkaline protease
Amount is the 1% of honey quality；The NaOH solution regulation pH to 8.7 using mass fraction to be 10% afterwards,
40 DEG C are incubated 2.5 hours, obtain alkaline solution；
Step 2, to add enzyme activity in described alkaline solution be the neutral protease of 150,000 u/g, described in
Addition is honey quality the 1% of property protease；The HC1 solution using mass fraction to be 10% afterwards is adjusted
Joint pH to 6.3,40 DEG C are incubated 2 hours, obtain neutral solution；
Step 3, in described neutral solution, add the pepsin that 5 enzyme activities are ten thousand u/g, described pepsin
The addition of enzyme is the 1% of honey quality；The HC1 solution regulation pH using mass fraction to be 10% afterwards
To 3,40 DEG C are incubated 3 hours, obtain acid solution；
Step 4, described acid solution is heated, be warming up to 85 DEG C, be incubated 15min, use 350 mesh filters
Paper filters, and obtains filtrate；
Step 5, in described filtrate add filtrate weight 2% activated carbon, 65 DEG C stirring decolouring 25min；
Filter with diatomite filter afterwards, obtain clear liquid；And at 55 DEG C by described clear
Liquid vacuum is concentrated into the 18% of primary liquid weight；Finally carry out lyophilization and obtain little point of described Mel entity
Sub-peptide powder.
The extracting method of the Mel entity small-molecular peptides that embodiment of the present invention 1-3 provides utilizes compound biological enzyme enzyme
Solution technology, the small-molecular peptides molecular weight obtained is mainly distributed on 180-480 dalton, mostly be dipeptides, tripeptides,
The small-molecular peptides such as tetrapeptide, belong to small molecule structure, have the feature utilized that is easily absorbed by the body.Mel is also
Can improve looks, facilitating digestion, the small-molecular peptides in Mel can improve body immunity, protection painstaking effort
Pipe, and can quickly be absorbed by the body, there is the effectiveness that well keeps healthy.
The present invention is not limited to above-mentioned preferred forms, and anyone can draw it under the enlightenment of the present invention
His various forms of products, no matter but on its formula or component, make any change, every have and the application
Technical scheme as same or like, within all falling within protection scope of the present invention.
Claims (8)
1. the extracting method of a Mel entity small-molecular peptides, it is characterised in that: comprise the steps:
Step 1, Mel is mixed with water, be heated with stirring to 50-80 DEG C, be incubated 15min, then lower the temperature
To 40 DEG C, adding alkaline protease, regulate pH to 8-9,40 DEG C are incubated 2-3 hour, obtain alkalescence
Solution；
Step 2, in described alkaline solution add neutral protease, regulate pH to 6-7,40 DEG C of guarantors
Temperature 2-3 hour, obtains neutral solution；
Step 3, in described neutral solution add pepsin, regulate pH to 2.5-3.7,40 DEG C of guarantors
Temperature 2-3 hour, obtains acid solution；
Step 4, described acid solution is heated, be warming up to 80-90 DEG C, be incubated 10-20min, filter
Obtain filtrate；
Step 5, in described filtrate, add activated carbon decolour, afterwards through filtering, concentrating, dry,
Obtain Mel entity small-molecular peptides powder.
The extracting method of Mel entity small-molecular peptides the most according to claim 1, it is characterised in that:
In step 1, it is that 1:8-13 mix with water according to mass ratio by described Mel.
The extracting method of Mel entity small-molecular peptides the most according to claim 1, it is characterised in that:
In step 1, adding the alkaline protease that enzyme activity is 500,000 u/g, the addition of described alkaline protease is
The 1% of honey quality.
The extracting method of Mel entity small-molecular peptides the most according to claim 1, it is characterised in that:
In step 2, adding the neutral protease that enzyme activity is 150,000 u/g, the addition of described neutral protease is
The 1% of honey quality.
The extracting method of Mel entity small-molecular peptides the most according to claim 1, it is characterised in that:
In step 3, adding the pepsin that enzyme activity is 50,000 u/g, described pepsic addition is Mel
The 1% of quality.
The extracting method of Mel entity small-molecular peptides the most according to claim 1, it is characterised in that:
The NaOH solution regulation pH using mass fraction to be 10% in step 1；In step 2, employing mass fraction is
The HC1 solution regulation pH of 10%；The HC1 solution regulation pH using mass fraction to be 10% in step 3.
The extracting method of Mel entity small-molecular peptides the most according to claim 1, it is characterised in that:
Step 4 use 300-400 mesh gauze or filter paper filter.
The extracting method of Mel entity small-molecular peptides the most according to claim 1, it is characterised in that:
In step 5, in filtrate, add the activated carbon of filtrate weight 1-2%, at 60-70 DEG C of stirring decolouring 20-30min；
Filter with diatomite filter afterwards, obtain clear liquid；And at 55 DEG C by described clear
Liquid vacuum is concentrated into the 15-20% of former clear liquid weight；Finally carry out being spray-dried or lyophilization
Obtain described Mel entity small-molecular peptides powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610190973.5A CN105767451A (en) | 2016-03-30 | 2016-03-30 | Extraction method of honey solid small molecular peptides |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610190973.5A CN105767451A (en) | 2016-03-30 | 2016-03-30 | Extraction method of honey solid small molecular peptides |
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| Publication Number | Publication Date |
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| CN105767451A true CN105767451A (en) | 2016-07-20 |
Family
ID=56392285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201610190973.5A Pending CN105767451A (en) | 2016-03-30 | 2016-03-30 | Extraction method of honey solid small molecular peptides |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371310A (en) * | 2018-01-02 | 2018-08-07 | 六安市叶集区武妹养蜂专业合作社 | A kind of processing method of honey |
| CN109652487A (en) * | 2019-01-21 | 2019-04-19 | 国药肽谷有限公司 | A kind of extracting method with the honey peptide for improving cardiovascular and cerebrovascular disease |
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| CN101418038A (en) * | 2008-11-14 | 2009-04-29 | 华子昂 | Highland barley peptide and preparation method thereof |
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| CN102499376A (en) * | 2011-12-29 | 2012-06-20 | 山东东阿阿胶股份有限公司 | Active small-molecule donkey-hide gelatin mixture and preparation method and application thereof |
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| CN1660417A (en) * | 2004-09-30 | 2005-08-31 | 周长信 | Formula of raw material pollen cream of nourishing face and preparing method |
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| CN108371310A (en) * | 2018-01-02 | 2018-08-07 | 六安市叶集区武妹养蜂专业合作社 | A kind of processing method of honey |
| CN109652487A (en) * | 2019-01-21 | 2019-04-19 | 国药肽谷有限公司 | A kind of extracting method with the honey peptide for improving cardiovascular and cerebrovascular disease |
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Application publication date: 20160720 |