CN101269090B - Method of preparing low molecular weight colla carapaeis corii asini hydrolysate - Google Patents
Method of preparing low molecular weight colla carapaeis corii asini hydrolysate Download PDFInfo
- Publication number
- CN101269090B CN101269090B CN2008100972845A CN200810097284A CN101269090B CN 101269090 B CN101269090 B CN 101269090B CN 2008100972845 A CN2008100972845 A CN 2008100972845A CN 200810097284 A CN200810097284 A CN 200810097284A CN 101269090 B CN101269090 B CN 101269090B
- Authority
- CN
- China
- Prior art keywords
- donkey
- enzyme
- hide gelatin
- corii asini
- molecular weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a preparation method of a low-molecular-weight colla corii asini hydrolyzate and belongs to the field of medical chemistry. The preparation method includes the following steps: firstly, pure colla corii asini without any auxiliary ingredients is adjusted to a system pH value between 5 and 9 under a constant temperature condition of 30 to 45 DEG C, and then enzyme is added in to have a hydrolysis reaction for 1 to 2 hours; secondly, enzyme is killed through heating; thirdly, colla corii asini hydrolyzate is obtained after cooling or a powder product is obtained through vacuum freeze drying; wherein, the enzyme is one, two or the compound of more than two of pepsin, trypsinase, papain, AS1.398 neutral protease and ficin. The preparation method of a low-molecular-weight colla corii asini hydrolyzate adopts pure colla corii asini as the ingredient and the generated product has high purity and is easier to be absorbed by the human body.
Description
Technical field:
The invention belongs to the medical chemistry field, the donkey-hide gelatin that particularly a kind of warp does not add any auxiliary material is the method for the low-molecular-weight donkey-hide gelatin hydrolyzate of feedstock production.
Background technology:
Donkey-hide gelatin is commonly called as Colla Corii Asini, is to boil the black Gelatinous plate that forms with clear water behind the donkey hide unhairing.The donkey-hide gelatin that traditional method is prepared is a molecular weight several ten thousand, and human body is difficult for absorbing, particularly for the patient who is under the morbid state since digestive function own a little less than, influenced giving full play to of donkey-hide gelatin drug effect to a certain extent; In addition, prepare in the process at donkey-hide gelatin, for the ease of final moulding, generally can in donkey-hide gelatin, add some auxiliary materials, the net result of doing like this is that this donkey-hide gelatin is bigger in viscosity after molten, and the part composition does not even dissolve, thereby makes the loss of part drug effect.
The patent No. is that the Chinese patent of CN1237421A discloses and a kind ofly utilizes enzyme that donkey-hide gelatin is hydrolyzed to the small molecules product to process liquid donkey-hide gelatin, takes and absorbs thereby be beneficial to human body.The patent No. is that the Chinese patent of CN1807653A then adopts specific prozyme, the control hydrolysis degree, thus obtained the substruction of donkey-hide gelatin collagen and the small molecules donkey-hide gelatin collagen peptide product that character is not damaged.Yet though above-mentioned patent has all obtained certain achievement, its preparation raw material has all used the donkey-hide gelatin finished product of handling through auxiliary material, when preparation hydrolysis donkey-hide gelatin, need these donkey-hide gelatin products be dissolved, owing to wherein contain auxiliary material, thereby be unfavorable for hydrolysis reaction.
Summary of the invention:
The purpose of this invention is to provide a kind of method that adopts the pure donkey-hide gelatin production small molecular weight donkey-hide gelatin hydrolyzate of no any auxiliary material, the donkey-hide gelatin hydrolyzate that obtains through this method is more conducive to absorption of human body.
Method of the present invention comprises following steps: (1) will not have the pure donkey-hide gelatin of any auxiliary material under 30～50 ℃ of constant temperatures; Regulation system pH value is to suitable value; Add enzyme, the reaction 1～2 hour that is hydrolyzed, (2) add the heat kill enzyme; (3) obtain the donkey-hide gelatin hydrolyzed solution through cold filtration; Or get powder-like product through vacuum lyophilization, wherein, described enzyme is meant one or both or the two or more mixtures in stomach en-, trypsinase, papoid, AS1.398 neutral protease, the ficin.
Wherein, the add-on of enzyme is 1 ‰～2% of a donkey-hide gelatin quality in the step (1).
Can add a kind of enzyme reaction certain hour earlier in the step (1), carry out the first step hydrolysis and reduce the donkey-hide gelatin molecular weight, add another kind of enzyme reaction certain hour again, molecular weight is further reduced.
The enzyme that step (1) adds also preferably papoid and trypsinase be the compsn that combines at 1: 2 by mass ratio, pH value of reaction system 5～8.5.
The enzyme that adds in the step (1) is the compsn that combines at 1: 1 by mass ratio of papoid and AS1.398 neutral protease preferably, the pH value 5～8 of reaction system.
The method of use of combination enzyme is reacted for carrying out the donkey-hide gelatin hydrolysis after mixing with combination one or more single enzymes in the enzyme or adding another kind of single enzyme with a kind of single enzyme wherein again after with the donkey-hide gelatin hydrolysis earlier.
The present invention adopts the purified donkey-hide gelatin liquid of no any auxiliary material, and hydrolysis becomes the hydrolysis donkey-hide gelatin of molecular weight less than 10KD under the effect of proteolytic enzyme.This product does not influence the pharmaceutical performance of donkey-hide gelatin, and is very easily water-soluble at normal temperatures, because reducing of molecular weight makes donkey-hide gelatin be absorbed by the body more easily, promotes the performance of its drug effect simultaneously.The hydrolysis prods that adopts the present invention to make, no any residue has been avoided the loss of drug effect in dissolution process.
Compare prior art, it is raw material that the present invention adopts pure donkey-hide gelatin liquid, has the following advantages: (1) has avoided the adding of partial supplementary material to influence the performance of donkey-hide gelatin drug effect; (2) avoided in the donkey-hide gelatin hydrolytic process, auxiliary material has improved efficient to the influence of hydrolysis reaction; (3) avoid donkey-hide gelatin dissolved energy expenditure again, reduced cost.
Embodiment:
Below be donkey-hide gelatin hydrolysate exemplary embodiments of the present invention, but those skilled in the art should be appreciated that of the present invention being embodied in accordance with being equal under the situation of substitution principle, are not limited to these embodiment.
Embodiment 1
Get 1000g donkey-hide gelatin liquid and place under 40 ℃ of constant temperatures, system pH is 7.0, adds 3g papoid reaction 2 hours, adds the heat kill enzyme afterwards and keeps 30 minutes, makes the thorough inactivation of proteolytic enzyme.Quench liquid through coarse filtration and smart filter, obtains the donkey-hide gelatin hydrolyzate solution successively.The liquid that obtains is carried out lyophilize obtain buff powder.Its hydrolysate molecular weight is below 10KD.
Embodiment 2
Get 1000g donkey-hide gelatin liquid and place under 45 ℃ of constant temperatures, system pH is 7.5, adds 2g trypsinase reaction 1 hour, adds the heat kill enzyme afterwards and keeps reaction 30 minutes, makes the thorough inactivation of proteolytic enzyme.Quench liquid through coarse filtration and smart filter, obtains the donkey-hide gelatin hydrolyzate solution successively.The liquid that obtains is carried out lyophilize can obtain buff powder.Its hydrolysate molecular weight is below 10KD.
Embodiment 3
Get 1000g donkey-hide gelatin liquid and place under 40 ℃ of constant temperatures, system pH is 5.0, adds the combination enzyme (1: 1) of 5g papoid and AS1.398 neutral protease, reacts 1 hour, and system adds the heat kill enzyme and keeps reaction 30 minutes afterwards, makes the thorough inactivation of proteolytic enzyme.Quench liquid through coarse filtration and smart filter, obtains the donkey-hide gelatin hydrolyzate solution successively.It is carried out spraying drying obtain buff powder.Its hydrolysate molecular weight is below 5KD.
Embodiment 4
Get 1000g donkey-hide gelatin liquid and place under 30 ℃ of constant temperatures, system pH is 7.0, adds 3g AS1.398 neutral protein enzyme reaction 1.5 hours, adds the heat kill enzyme afterwards and keeps reaction 30 minutes, makes the thorough inactivation of proteolytic enzyme.Quench liquid through coarse filtration and smart filter, obtains the donkey-hide gelatin hydrolyzate solution successively.It is carried out spraying drying obtain golden yellow powder.Its hydrolysate molecular weight is below 10KD.
Embodiment 5
Get 1000g donkey-hide gelatin liquid and place under 45 ℃ of constant temperatures, system pH is 8.0, adds 3g papoid and trypsinase mixture (1: 2) reaction 2 hours, adds the heat kill enzyme afterwards and keeps reaction 30 minutes, makes the thorough inactivation of proteolytic enzyme.Quench liquid through coarse filtration and smart filter, obtains the donkey-hide gelatin hydrolyzate solution successively.It is carried out spraying drying obtain buff powder.Its hydrolysate molecular weight is below 5KD.
Embodiment 6
Get 1000g donkey-hide gelatin liquid and place under 40 ℃ of constant temperatures, system pH is 5.0, adds the 2.5g papoid, reacts 1 hour, and system adds the heat kill enzyme and keeps reaction 30 minutes afterwards, makes the thorough inactivation of proteolytic enzyme.Quench liquid obtains hydrolyzate solution through coarse filtration; Under 35 ℃ of constant temperatures, system pH is 7.0 again, adds 2.5g AS1.398 neutral protease, reacts 1 hour, and system adds the heat kill enzyme and keeps reaction 30 minutes afterwards, makes the thorough inactivation of proteolytic enzyme.Quench liquid through coarse filtration and smart filter, obtains the donkey-hide gelatin hydrolyzate solution successively.It is carried out spraying drying obtain buff powder.Its hydrolysate molecular weight is below 5KD.
Claims (1)
1. the preparation method of a low molecular weight colla carapaeis corii asini hydrolysate, it is characterized in that: get 1000g donkey-hide gelatin liquid and place under 40 ℃ of constant temperatures, system pH is 5.0; Add the 2.5g papoid; Reacted 1 hour, system adds the heat kill enzyme and keeps reaction 30 minutes afterwards, makes the thorough inactivation of proteolytic enzyme; Quench liquid obtains hydrolyzate solution through coarse filtration; Under 35 ℃ of constant temperatures, system pH is 7.0 again, adds 2.5g AS1.398 neutral protease, reacts 1 hour, and system adds the heat kill enzyme and keeps reaction 30 minutes afterwards, makes the thorough inactivation of proteolytic enzyme; Quench liquid through coarse filtration and smart filter, obtains the donkey-hide gelatin hydrolyzate solution successively; It is carried out spraying drying obtain buff powder;
Its hydrolysate molecular weight is below 5KD.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100972845A CN101269090B (en) | 2008-04-21 | 2008-05-09 | Method of preparing low molecular weight colla carapaeis corii asini hydrolysate |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810104550.2 | 2008-04-21 | ||
| CN200810104550 | 2008-04-21 | ||
| CN2008100972845A CN101269090B (en) | 2008-04-21 | 2008-05-09 | Method of preparing low molecular weight colla carapaeis corii asini hydrolysate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101269090A CN101269090A (en) | 2008-09-24 |
| CN101269090B true CN101269090B (en) | 2012-07-04 |
Family
ID=40003482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100972845A Active CN101269090B (en) | 2008-04-21 | 2008-05-09 | Method of preparing low molecular weight colla carapaeis corii asini hydrolysate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101269090B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101851339B (en) * | 2010-05-19 | 2011-10-26 | 成都新际生物活性胶原开发有限公司 | Method for preparing donkey skin collagens |
| CN102090639B (en) * | 2010-09-06 | 2013-09-11 | 山东好当家海洋发展股份有限公司 | Sea cucumber colla corii asini polypeptide nutrient product and preparation method thereof |
| CN102021215B (en) * | 2010-11-11 | 2012-12-12 | 湖北远成药业有限公司 | Method for extracting gelatin peptides |
| CN102321716B (en) * | 2011-08-18 | 2015-01-14 | 江中药业股份有限公司 | Preparation method of donkey-hide gelatin oligopeptide |
| CN102499376B (en) * | 2011-12-29 | 2014-08-27 | 山东东阿阿胶股份有限公司 | Active small-molecule donkey-hide gelatin mixture and preparation method and application thereof |
| CN103655616B (en) * | 2013-09-25 | 2016-03-23 | 山东东阿阿胶股份有限公司 | The application improved in the medicine of hair quality prepared by Colla Corii Asini |
| CN106399430A (en) * | 2016-08-31 | 2017-02-15 | 哈哈驴（天津）国际贸易有限公司 | Production method for decocting donkey hoof glue by using fresh donkey hoofs |
| CN109984333A (en) * | 2019-01-28 | 2019-07-09 | 天津星宇航天生物科技有限公司 | A kind of civilian nutrient formulation of aerospace for being applicable in qi and blood and raising crowd |
| CN111329988A (en) * | 2019-11-30 | 2020-06-26 | 北京时代名媛生物科技有限责任公司 | Donkey-hide gelatin peptide buccal tablet rich in free arginine and preparation method thereof |
-
2008
- 2008-05-09 CN CN2008100972845A patent/CN101269090B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN101269090A (en) | 2008-09-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101269090B (en) | Method of preparing low molecular weight colla carapaeis corii asini hydrolysate | |
| CN102492760B (en) | Method for producing active Rana dybowskii collagen oligopeptide by using compound enzyme | |
| CN102174627B (en) | Method for preparing rapeseed bioactive peptide | |
| CN102775340B (en) | Method for synthesizing pyroglutamic calcium glutamate with shells serving as calcium sources | |
| CN101724677B (en) | Method for extracting collagen polypeptide and hydroxyapatite in fish scales by cooking hot extrusion | |
| CN101250574A (en) | Preparation method of cod skin collagen peptide with cascaded molecular weight | |
| CN103060406B (en) | Method for Extracting Low Molecular Weight Collagen from Livestock Bone | |
| CN104031963A (en) | Method for preparing hpyerglycemic peptide by using silkworm chrysalis | |
| CN103739663A (en) | Method for rapidly preparing small peptide amino acids by microwave-assisted protein acid hydrolysis | |
| CN103045705A (en) | Method for producing protein peptide composed of fish protein peptide and soybean peptide | |
| JP2737790B2 (en) | Food containing silk protein hydrolyzate and method for producing the same | |
| WO2003066683A1 (en) | Mucopolysaccharides and process for producing the same | |
| WO2004039994A1 (en) | Sodium chondroitin sulfate, chondroitin sulfate-containing material and processes for producing the same | |
| WO2011072596A1 (en) | Soluble collagen and preparation method and use thereof | |
| CN101130801A (en) | Preparation of active antihypertensive peptide product | |
| CN1844406A (en) | Method for preparing antihypertensive peptides by using proteins in shrimps | |
| CN107858390A (en) | A kind of method prepared by corn macromolecular polypeptides based on gastro-intestinal digestion | |
| CN105767451A (en) | Extraction method of honey solid small molecular peptides | |
| CN100571542C (en) | The preparation method of venison enzymolysis oral liquid | |
| CN107338277A (en) | A kind of method that ultrasonication wheat gluten prepares high F value oligopeptide | |
| CN101914210A (en) | Preparation process of succinylated gelatin | |
| JP2000270806A (en) | Sea tangle essence and its production | |
| CN111329771A (en) | Skin-protecting and face-refreshing essence and preparation method thereof | |
| CN111254176A (en) | Preparation method of egg white polypeptide | |
| CN106974108A (en) | A kind of defatted soybean meal prepares the optimize technique of small molecular protein peptide pig feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20080924 Assignee: FOSHAN WIN COSMETIC CO., LTD. Assignor: Beijing University of Chemical Technology Contract record no.: 2015440000010 Denomination of invention: Method of preparing low molecular weight colla carapaeis corii asini hydrolysate Granted publication date: 20120704 License type: Exclusive License Record date: 20150113 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model |